LOCUS       ACXX02000007            5925 bp    DNA     linear   BCT 14-MAR-2011
DEFINITION  Ruminiclostridium papyrosolvens DSM 2782 ctg58, whole genome shotgun
            sequence.
ACCESSION   ACXX02000007
VERSION     ACXX02000007.1
DBLINK      BioProject: PRJNA33587
            BioSample: SAMN00002727
KEYWORDS    WGS; GSC:MIGS:2.1; NONCONTIGUOUS_FINISHED.
SOURCE      Ruminiclostridium papyrosolvens DSM 2782
  ORGANISM  Ruminiclostridium papyrosolvens DSM 2782
            Bacteria; Firmicutes; Clostridia; Clostridiales;
            Hungateiclostridiaceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Mouttaki,H.,
            He,Z., Zhou,J., Hemme,C.L. and Woyke,T.
  TITLE     The Non-contiguous Finished genome of Clostridium papyrosolvens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L.,
            Mouttaki,H., He,Z., Zhou,J. and Hemme,C.L.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 5925)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Mouttaki,H.,
            He,Z., Zhou,J., Hemme,C.L. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type       :: bacteria_archaea
            project_name             :: Clostridium papyrosolvens DSM 2782
            collection_date          :: Missing
            lat_lon                  :: 57.162143 -2.719417
            depth                    :: Missing
            alt_elev                 :: Missing
            country                  :: United Kingdom
            environment              :: Mud
            num_replicons            :: Missing
            ref_biomaterial          :: DSM 2782, ATCC 35413, NCIB 11394
            biotic_relationship      :: Free living
            trophic_level            :: Chemoorganotroph
            rel_to_oxygen            :: Anaerobe
            isol_growth_condt        :: Missing
            sequencing_meth          :: WGS
            assembly                 :: Newbler v. 2.3 (pre-release)
            finishing_strategy       :: Finished
            GOLD Stamp ID            :: Gi02584
            Type Strain              :: Yes
            Greengenes ID            :: 15957
            Isolation Site           :: estuarine sediment, River Don,
            Source of Isolate        :: Christopher L. Hemme (hemmecl@ou.edu)
            Cell Shape               :: Rod-shaped
            Motility                 :: Motile
            Sporulation              :: Sporulating
            Temperature Range        :: Mesophile
            Temperature Optimum      :: 25C
            Gram Staining            :: Gram-
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Noncontiguous Finished
            Current Finishing Status :: Noncontiguous Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454; Illumina
            ##Genome-Assembly-Data-END##
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4085558
            Source DNA and Organism available from Christopher L. Hemme
            (hemmecl@ou.edu)
            Contacts: Christopher L. Hemme (hemmecl@ou.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            Aberdeenshire, Scotland
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Ruminiclostridium papyrosolvens DSM 2782"
                     /mol_type="genomic DNA"
                     /submitter_seqid="ctg58"
                     /strain="DSM 2782"
                     /isolation_source="estuarine sediment from River Don"
                     /type_material="type strain of Ruminiclostridium
                     papyrosolvens"
                     /db_xref="taxon:588581"
                     /country="United Kingdom: Aberdeenshire, Scotland"
     gene            1..2853
                     /locus_tag="Cpap_1693"
     CDS             1..2853
                     /locus_tag="Cpap_1693"
                     /inference="protein motif:PFAM:PF00759"
                     /note="PFAM: glycoside hydrolase family 9; Dockerin type 1;
                     type 3a cellulose-binding domain protein; KEGG:
                     cce:Ccel_1648 glycoside hydrolase family 9"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM3|GH9"
                     /protein_id="EGD47503.1"
                     /db_xref="GO:0003677"
                     /db_xref="GO:0030246"
                     /db_xref="InterPro:IPR001701"
                     /db_xref="InterPro:IPR001956"
                     /db_xref="InterPro:IPR002105"
                     /db_xref="InterPro:IPR018242"
                     /db_xref="InterPro:IPR018247"
                     /translation="MSRVTKGRKKRLSILLAIALLAGQIFSAVSVSAEAPPATFTPAEG
                     WENYNYFNFAEALQKSLYFYDAEKCGEAAGYDKGGKLEWRGSCHEADAKIPLKYTNLSA
                     AFLEKYKGLIDPDGDGTVDVHGGFHDAGDHVRFGLPQSYTAGTLGWGFYEFRDSYKAIG
                     EEEHIIDILKYFTDTFLRCSYLDKDGNLIAFCYMVGEGDVDHCYWGPPELYPEEYQKTR
                     PADFATAETPGSDVCASTAAALCTSYLNFKDSEPEYAEKCLKVAKAMYEFAKKYRGKAA
                     GDGYYTSDYDEDELAWAAVWLYQCTGNMDYIKDIDSVDEKGNYTGYMKRIIPETYNTNT
                     WYNSWTHCWDAVWAGTFLKLNELLPDNELFNFIASWNVEYLSGGDAKHKDPNDHNYYKT
                     SPAGYTMINGWGSARYNTAAQLCALVYMKHHPERTDFGEWAKKEMEYLMGRNPMGYSYI
                     VGYGYEQGLPFAHHPHHRAAHGSKTNSMNDPVEHRHILWGALSGGPDSSDYHIDSTTEY
                     AYNEVAVDYNAAFVGALAGLYKYYGQGQKPIANFPPKEPRTDDYYCEARVARETTDSTQ
                     VVLKIHNESSQPPHYETGMKARYYFNIKELLKSGQDIDDVIFSIEYDEQISMQQDPIKL
                     TGPFKWDEAGTYYYEFDWSGSKIYGDRELQISFRAKQDPNYLTHWDSSNDYSKKNLTTT
                     YAVTKNIPVYLDGTLVYGEEAPKLDPSTDPEDPDNTPPSISVSYKCGTADVMKNTIRSS
                     INIKNTGTVPINLSDIKLRYWFTNDGNQQNSFTCEYAALGSGNVTGNFSNISSPVPGAD
                     TYCEIGFTSAAGKLSPGGSTGAIPFRIEGASDYDKTNDYSYDSKMTELGDNTKMTGYIK
                     GELKYGVEPVVVNIKLGDVDGSGTIDSIDFALMKKHILGSTQLDSESKIRADVNKDNEV
                     NSIDLALLKKYLLGTISSFS"
     sig_peptide     1..102
                     /locus_tag="Cpap_1693"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 0.994) with cleavage site probability 0.823 at
                     residue 34"
     gene            3037..5925
                     /locus_tag="Cpap_1694"
     CDS             3037..5925
                     /locus_tag="Cpap_1694"
                     /inference="protein motif:PFAM:PF00759"
                     /note="PFAM: glycoside hydrolase family 9; type 3a
                     cellulose-binding domain protein; Dockerin type 1; KEGG:
                     cce:Ccel_1648 glycoside hydrolase family 9"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM3|GH9"
                     /protein_id="EGD47504.1"
                     /db_xref="GO:0030246"
                     /db_xref="GO:0048037"
                     /db_xref="InterPro:IPR001701"
                     /db_xref="InterPro:IPR001956"
                     /db_xref="InterPro:IPR002105"
                     /db_xref="InterPro:IPR018242"
                     /db_xref="InterPro:IPR018247"
                     /translation="MNRFIKARKVLNRKSIAAMLLSVSMIAGQFLLSSNALAMENTDSN
                     SGTTSTAESKFTPKEGWEDYNYFNFAEALQKSIYFYDAEKCGPGVSGGKIEWRGDCHVG
                     DCNIPLKNTSLSAEFIEKYRKILDPDGDGTVDVHGGFHDAGDHVRFGLPQSYTAGTLGW
                     SFYEFRDAFKKSGQEEHMIDILKTFTDTFLRCSYLDENGDLIAFCYMVGEGDLDHSYWG
                     PPELYPDNIPRPADFATAETPGSDVCGSTAAALATSYLNFKDDEPDYAKKCLTVAKAMY
                     KFAKENRGMSNGDGYYTSAYDDDEMAWAAVWLYTCTGNMDYINDIMSVSEDGKYYTGYM
                     KKIIPDTFNTNMWFNTWTHCWDAVWGGVFVELNALFPDNKLYDYIARWNTEYLSGGMAK
                     HKEANDNGYTSTSPAGYTMINGWGSARYNTAAQLCALVYMEHHPDRTDFGAWAKNQMEY
                     LMGRNPMGYSYIVGYGYEHGLPSAKHPHHRAAHGSKTLSMNDPPEHRHIIWGALVGGPD
                     KEDYHQDVTTDFVYNEVAVDYNAAFVGACAGLYKYYGEGQQVIPNFPPKEPKTDDYYCE
                     ARIEREIKESSQVVIKLHNESSQPPHYETGMKARYFFNISELLACGQSIKDVEMTIAYD
                     EQISLQQEPITYRGPIKWDDAGTYYYEFDWSGREIYGDRELAFTLTAKQDSNYMTHWNP
                     TNDWSRKNLTSDYVLTKNVPVYMNGVLVYGEEAPKLTPTNPDDNPDTTPPSIKVQYKYG
                     AAHNLTNTLRSTVNIVNNGTVPVNLSDIKLRYWFTCDGSEQNTFACEYAPCGAENVTGN
                     CYKVDNAVGGADTYCEISFKDAAGKLVPGASTGDIPFRIDGSSDYDHTNDYSCNPKMES
                     AYGDNEKITAYIKGELKYGVEPVVVNIKLGDVDGSGTVDAIDFALLKKFLLGSLQLDSE
                     SKIRADVNKDDSVNAIDLALLKKYILGTASEF"
     sig_peptide     3037..3153
                     /locus_tag="Cpap_1694"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 0.994) with cleavage site probability 0.991 at
                     residue 39"
ORIGIN
        1 atgtcaagag ttacaaaagg aaggaaaaag cgactgtcga tcttgctggc tattgcgctt
       61 ttagctggac agattttttc ggcagtttcg gtatcggcgg aagctccgcc agctacgttt
      121 actccggctg agggctggga aaactacaac tatttcaact ttgcagaagc actacagaaa
      181 tcactttatt tttacgatgc agagaaatgc ggcgaagcag ccggttatga caaaggagga
      241 aagcttgaat ggaggggttc atgccatgaa gcagatgcaa agataccctt gaagtacact
      301 aacctgtccg ctgctttttt agaaaagtat aaaggtttaa ttgatcctga cggagacggt
      361 acagtagatg tacacggagg cttccacgat gcaggagacc atgtccgctt cggtcttcca
      421 cagagctata ccgccggtac ccttggctgg ggcttctatg aattcaggga ttcctacaag
      481 gcaataggcg aagaagaaca tataattgat attttgaaat actttaccga taccttcttg
      541 cgctgttcct atcttgataa ggatggaaat ctaattgcat tttgctatat ggtaggagaa
      601 ggtgacgttg accactgtta ctggggcccg ccggaacttt atcccgaaga gtatcagaag
      661 accagacctg ctgattttgc aactgcagaa actccgggaa gtgatgtatg tgccagtact
      721 gccgcagcac tttgtacatc atatctgaac tttaaagatt cagagcctga atatgcagaa
      781 aagtgcttga aggttgcaaa agccatgtat gaatttgcta aaaaatacag aggcaaggcc
      841 gctggtgatg gctactatac ttctgattat gatgaagacg agctggcatg ggctgctgta
      901 tggctttatc agtgtacagg aaatatggat tacataaaag atattgattc agttgatgaa
      961 aagggaaact atacagggta tatgaagaga attatacctg aaacctataa taccaatacc
     1021 tggtataatt catggactca ttgctgggat gcagtatggg caggaacctt cttaaagctc
     1081 aatgaattgt tacccgacaa tgaattattc aactttattg caagctggaa cgtagaatat
     1141 ttgtccggcg gtgatgcaaa gcataaagat ccaaatgacc ataactatta taaaacatca
     1201 cctgccggat ataccatgat aaacggctgg ggttctgctc gttacaatac tgctgcacag
     1261 ttatgtgctc ttgtttatat gaagcatcat cctgaaagaa ctgatttcgg tgaatgggca
     1321 aagaaggaga tggaatacct tatgggaaga aatcctatgg gatattcata tatagtaggc
     1381 tacggctatg aacagggact tccttttgca catcatccac atcacagagc agcacatggc
     1441 tcaaaaacca acagtatgaa tgaccctgtg gaacacagac atatattgtg gggcgctttg
     1501 tcaggggggc cggattcaag tgattatcat atagattcaa ctaccgagta tgcttacaat
     1561 gaagttgcag ttgactataa tgctgctttt gttggcgctt tagccggatt gtataaatac
     1621 tacggacagg gacaaaaacc aatagccaac ttcccgccaa aggaaccaag aactgatgat
     1681 tactactgtg aagctcgtgt agcaagagaa actactgaca gtacacaggt agttttaaaa
     1741 attcataatg aatccagtca accacctcat tacgaaacag gcatgaaggc aagatattac
     1801 tttaacatta aagaattgtt aaaaagtgga caagatatag acgatgtaat attttcaata
     1861 gagtatgatg agcagatttc catgcagcag gatcctatca agctcacagg gcctttcaaa
     1921 tgggatgaag caggaacata ctattatgaa tttgattgga gcggaagtaa aatatatgga
     1981 gacagagaac ttcaaatatc tttccgtgca aagcaggatc caaattatct gacccattgg
     2041 gattcttcaa acgactacag taagaaaaac ctgacaacca cgtatgcagt aacaaagaat
     2101 atacctgtat acttggacgg gactctggta tatggagaag aagctccaaa gcttgatccg
     2161 tctaccgacc ctgaagatcc cgataataca ccgccatcaa taagtgtctc ctacaagtgc
     2221 ggtactgcag atgtgatgaa aaataccata aggtcttcaa tcaatataaa gaataccgga
     2281 acagttccca taaatctgtc tgatataaag cttcgttatt ggtttacaaa tgacgggaac
     2341 cagcagaact cctttacatg tgaatacgca gcgttgggaa gcggcaatgt cacaggtaat
     2401 ttctccaata taagcagtcc tgttccgggt gccgacacct attgtgaaat cggttttaca
     2461 agtgcagccg gaaagctctc accgggagga agtaccgggg ctataccatt cagaatagag
     2521 ggcgcgtcag attatgacaa gacaaacgac tattcatatg attcaaaaat gactgaactt
     2581 ggcgataata caaaaatgac aggttacata aagggtgaac tcaagtacgg agttgaacct
     2641 gtggttgtta atatcaagct cggagatgta gacggcagtg gaactataga ttctatagat
     2701 tttgcattaa tgaaaaaaca tattcttggt tctacgcaat tggactcaga gagcaaaatc
     2761 agagcggacg taaataaaga taatgaagtt aattcaattg accttgcatt actcaaaaag
     2821 tatcttctgg gaacaatatc ttcattctcg tagaagacta acatctgtga taactcaaaa
     2881 ttatttgaat ctaaaataat ctataaagac cgtactctta ttcaaattga atgtatgtaa
     2941 actagagagt acggtctttt gtaaaatcat gtttctgaaa aaattttaca tctttaattt
     3001 ttcttataat aaattctcaa gagggaggta ttaagcttga acagatttat aaaggctaga
     3061 aaagttttaa atcggaaaag tattgcagca atgcttttga gtgttagcat gattgctgga
     3121 caatttctat tatcgagtaa tgcattagca atggaaaata ctgattctaa ttcaggtact
     3181 acttcaacag cagaatcaaa attcactcct aaagaaggat gggaagatta taactacttt
     3241 aattttgctg aagcattgca aaaatcaatt tacttctacg atgcagaaaa atgcggacca
     3301 ggggtttcag gaggtaaaat tgaatggaga ggcgattgcc atgtgggaga ttgcaatatt
     3361 ccccttaaaa acacttctct ttccgctgaa ttcatagaaa agtatagaaa aatactcgac
     3421 cctgatgggg atggaacagt agatgtacat ggtggattcc atgatgctgg agaccatgta
     3481 cggtttggac ttcctcagag ctatacagca ggtactcttg gatggagttt ctatgaattt
     3541 agagatgcct tcaagaaatc agggcaagaa gaacatatga ttgatatttt aaagaccttt
     3601 actgatacct ttttacgttg ctcctatctg gatgaaaatg gagatttgat tgctttctgt
     3661 tacatggttg gtgaagggga tttagatcac tcctattggg gaccacctga attatatccc
     3721 gataatatac caagaccagc agattttgcc actgctgaaa ctcctggtag tgatgtttgt
     3781 ggtagtactg ctgcagcact tgccacatct tatttgaatt ttaaggatga cgagcctgat
     3841 tatgcgaaaa agtgtttgac tgttgcaaag gctatgtata aattcgcaaa ggaaaacaga
     3901 ggaatgtcta atggtgatgg ttactataca tcagcatatg acgatgatga aatggcttgg
     3961 gctgcagtat ggttatatac ttgcactggt aatatggact atataaatga tattatgtca
     4021 gtatcagaag acggtaaata ttatactgga tatatgaaaa aaattatacc agataccttt
     4081 aacacaaata tgtggttcaa cacatggact cattgttggg atgcagtatg gggaggagtg
     4141 ttcgtagagc tgaatgcttt attcccagac aataagttat atgactacat tgctcgttgg
     4201 aatacagagt atttgtctgg tggtatggcg aagcataagg aagccaacga taatggctat
     4261 acttcaacat cacctgcagg atatacgatg ataaatggat ggggatcagc tcgttacaat
     4321 acggctgctc aattatgcgc acttgtatat atggaacacc accctgatag aacagatttt
     4381 ggagcatggg caaagaatca gatggaatac cttatgggaa gaaatccaat ggggtactca
     4441 tacatagttg ggtacggcta tgaacatggc ttgccttctg caaaacatcc gcatcacagg
     4501 gcggctcatg gatcaaaaac attgagtatg aatgacccgc cagaacacag gcatattata
     4561 tggggtgctc ttgttggcgg gcctgataag gaggattacc atcaggatgt aacaactgat
     4621 ttcgtttaca atgaggttgc tgttgactat aatgctgcat ttgtaggtgc ttgtgcggga
     4681 ttatataaat actacggtga gggacagcag gtaataccta attttccacc aaaagaacca
     4741 aagactgacg attactattg cgaggcacgt atagaaagag agattaagga aagctcacag
     4801 gtagttatta aattacacaa tgagtcaagc cagcctccac attatgaaac aggtatgaaa
     4861 gcaagatatt tctttaatat tagtgaactg cttgcatgcg gacagtccat aaaggatgtt
     4921 gaaatgacta ttgcttatga tgaacagatt tcactgcagc aggaacctat tacctataga
     4981 ggtccaataa agtgggatga tgctggaacc tattattatg aatttgattg gagcggcaga
     5041 gaaatatatg gggatagaga gttggcattt acgttaacag caaaacagga ttcaaattat
     5101 atgacacatt ggaacccaac taatgactgg agcagaaaga atcttaccag tgattatgtg
     5161 ttaactaaaa atgttcccgt atacatgaat ggcgttctag tatatgggga ggaagctcca
     5221 aaacttactc caacaaatcc tgatgataac ccagatacta caccgccttc aataaaggtt
     5281 cagtataagt atggcgcagc tcacaaccta acaaatacat taaggtctac agttaatata
     5341 gtaaataatg gaactgttcc tgtaaattta tcagacataa agctacgcta ttggtttaca
     5401 tgtgatggta gtgagcagaa tacatttgca tgtgaatatg caccttgcgg tgctgaaaat
     5461 gtaacgggta attgctacaa agtagacaat gctgtggggg gtgccgatac atattgtgag
     5521 atttcattta aagatgctgc aggcaaactt gtaccaggcg caagcacagg agatatacct
     5581 tttaggatag atggctcgtc cgattatgac catacaaatg attattcatg taatcctaaa
     5641 atggaaagtg cttatgggga taatgaaaaa atcactgctt atatcaaggg tgaactcaag
     5701 tacggagttg aacctgtggt tgttaatatc aagctcggag atgtagatgg tagtggtact
     5761 gtagatgcta ttgactttgc attactgaaa aaatttctct taggttcttt acaattggat
     5821 tcagagagca aaatcagagc agatgtaaat aaggatgatt cagttaatgc aatagacctt
     5881 gcattactca aaaagtatat ccttggaaca gcatctgaat tttag
//
