LOCUS       CP028460               14878 bp    DNA     linear   BCT 22-OCT-2018
DEFINITION  Bifidobacterium animalis subsp. animalis strain CNCM I-4602
            chromosome, complete genome.
ACCESSION   CP028460
VERSION     CP028460.1
DBLINK      BioProject: PRJNA445301
            BioSample: SAMN08774047
KEYWORDS    .
SOURCE      Bifidobacterium animalis subsp. animalis
  ORGANISM  Bifidobacterium animalis subsp. animalis
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 14878)
  AUTHORS   Egan,M., Bottacini,F., O'Connell Motherway,M., Casey,P.G.,
            Morrissey,R., Melga,S., Faurie,J.-M., Chervaux,C., Smokvina,T. and
            van Sinderen,D.
  TITLE     Staying alive: Growth and survival of Bifidobacterium animalis
            subsp. animalis under in vitro and in vivo conditions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14878)
  AUTHORS   van Sinderen,D., Chervaux,C. and Smokvina,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2018) School of Microbiology, University College
            Cork, Western Road, Cork ND, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SMRT Analysis v. 2.3
            Expected Final Version :: yes
            Genome Coverage        :: 400.0x
            Sequencing Technology  :: Pacbio SMRT sequencing
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14878
                     /organism="Bifidobacterium animalis subsp. animalis"
                     /mol_type="genomic DNA"
                     /strain="CNCM I-4602"
                     /host="Homo sapiens"
                     /sub_species="animalis"
                     /db_xref="taxon:302912"
                     /country="France"
                     /collection_date="01-Oct-2014"
     gene            complement(1..891)
                     /locus_tag="CNCMI4602_1329"
     CDS             complement(1..891)
                     /locus_tag="CNCMI4602_1329"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AYN24174.1"
                     /translation="MKGIILAGGSGTRLYPLTTVTSKQLLPVYDKPMIFYPLSALMMAG
                     IRDILIISTPKDLPNFERLLGDGSRYGISLQYKVQPSPDGLAQAFLLGEDFIGDDACAL
                     VLGDNIFYGNGLGRLLRKAAALEHGATVFGYYVDDPERYGVVEFDKDHKAVSIVEKPEH
                     PASNYAVTGLYFYDNRVVDFAKQVKPSPRGELEITDLNRMYLEDGSLHVQTLGRGYAWL
                     DTGTMDSLFEAGEFVRTVEHAQGLPISVIEEIAYENHWIDRDQLLAAAERYGKSPYGKH
                     LLDVADHRFLSTIDD"
     gene            complement(905..2356)
                     /locus_tag="CNCMI4602_1330"
     CDS             complement(905..2356)
                     /locus_tag="CNCMI4602_1330"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AYN24175.1"
                     /translation="MEFEKELKVTETNIPGLLVFDLPVHGDNRGWFKENWQKAKMTALG
                     LPKDFQPVQNNVSFNAKRGVTRGIHAEPWDKFISLATGEIFGAWVDLRPGDTFGQVYTT
                     RLDPSKAIYVPRGVGNSFQALEDGTAYTYLVNAHWSLEQKKTYTFVNLADPELNIQWPI
                     PLEQAELSKADLHHPMLKDAKPMDPKRTLVTGANGQLGQSIRKYVEEHDLKGFEFTDID
                     EFDFSDPAAYEGYDWSLYGTIINAGAFTAVDKAETPEGRVTAWKANALGPALLTKVAAE
                     HNLTLVHVSSDYVFDGTAKVHDEDEAFSPLGVYGQTKAAGDIAVTNAPKHYIVRSSWVI
                     GNGHNFVKTMMMLSNKVSDPNDALNEVTVVDDQYGRLTFTDDMAAAIFHLLDDEDPYGT
                     YDLTGSGDVVSWAQIAAEVFAMTNGNGDKVRPISTEQYFENAKAPVSPRPTNSTLDLAK
                     IEETGYETTDWHDSLKAYVEAELKK"
     gene            complement(2432..3451)
                     /locus_tag="CNCMI4602_1331"
     CDS             complement(2432..3451)
                     /locus_tag="CNCMI4602_1331"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AYN24176.1"
                     /translation="MTEAFIPKNIIVTGGCGFIGSNFVHYVYNNHPDVHITVLDALTYA
                     GNLENIKPILGDRVEFVHGNICDAQLLDELVPGHDAIVHYAAESHNDNSIANPEPFVKT
                     NVEGTFRLLEAARKYDVRFHHISTDEVYGDLALDDPAKFTEETPYHPSSPYSSTKASSD
                     MLVRAWHRTYGLRTTISNCSNNYGPYQHVEKFIPRQITNIIEGIRPKLYGDGLNVRDWI
                     HTEDHSSAVWTILTTGRLGETYLIGANGERNNLTVLHDILRVMGQPEDAFDWVKDRPGH
                     DRRYAIDSTKLQTELGWRPTHTDFESGLRQTVQWYIDNPQWWEPAKAATEAKYKAQGQ"
     gene            3647..5083
                     /locus_tag="CNCMI4602_1332"
     CDS             3647..5083
                     /locus_tag="CNCMI4602_1332"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8KUK6|2.A.66.2.16"
                     /protein_id="AYN24177.1"
                     /translation="MSKSLVKNSFFNVVYQLLNVLFPLITAPYVARVLLPENVGKVAVA
                     QNWAQYFIVFAALGLPTYAVRETAQHRKNKQELDKAFSELLMINTISTTISVIAYIIML
                     FTFGTFRENIALYLAVGAGILFNYITIDWLYQGIEDYGYIAMRSFIVKLLSLIALLVLV
                     RNRNDFVIYALISTCAVGANNILNVLHLPKIGVHLTFRNLTFKQHVKPIMIFFANSISV
                     RLYTLLNVTFLGIFSTDDAVAFYSNSDKVVRIIITVIAGIGNVLLPRLSIYAKRGEKKR
                     CSNIVSSVFNILFFIFVPTGIGLILMSKEIVLILFGSAYAPSILTLQIMSLLIYTLGFN
                     NLFVSINLAFAKDKEYLICSISGALSCLVLNYVLIPVLQQNGTAIASVLSEGIVTGVAI
                     FFSAQTIKLHFDKKIIAKTILASILMAVVVYCFKLLISNLILCLIASVGFGSLTYIIIS
                     WLLKNPVLFQIINLTLKCRK"
     gene            complement(5090..5656)
                     /locus_tag="CNCMI4602_1333"
                     /note="Glycosyl transferase, frameshifted"
                     /pseudo=""
     gene            complement(5608..5970)
                     /locus_tag="CNCMI4602_1334"
                     /note="Glycosyl transferase, frameshifted"
                     /pseudo=""
     gene            complement(5989..7002)
                     /locus_tag="CNCMI4602_1335"
     CDS             complement(5989..7002)
                     /locus_tag="CNCMI4602_1335"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P75905|4.D.1.1.3"
                     /protein_id="AYN24178.1"
                     /translation="MPEISVIIPVYNQEEYVGACMDSLLDQSFEDFEVLAVNDGSTDAS
                     ASILEGYEQRDGRVQLIDQNNSGVSVARNNGLSHAKGEWVCFIDPDDYVAPDYLETLLK
                     ATERNPDIVMSTCVAFDDQHSARQHFFPESFTARAADEKVELFRQLMDGSYAQPKGFVT
                     AIGVPWGKLYRHAFLQEHGLQFDAALPRMQDNLFNMQAFQLAHEIVYVDYAGYYYRMGG
                     LSARTYKNNAKGLYHPAIDERARLMHEYHLDGDATLLWAWNEEQVNLYFQELKAVIMLA
                     DPQSAKQAARTRAQALGPRLDQIDVSALSPAIRFKYTMMRNAVLRSLLVHALRAKG"
     gene            complement(6995..8341)
                     /locus_tag="CNCMI4602_1336"
     CDS             complement(6995..8341)
                     /locus_tag="CNCMI4602_1336"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AYN24179.1"
                     /translation="MTDTTETVQRQAAYTPRIHEVVNTLMDYALVLAIIYNSNSLWALA
                     DDNFSLSCFVLLAVCLAYMLCCWREARHRLHRVGSAMLVLVLATVIGQLVMAVSHGGSG
                     YTDGIWFQYALIVPALMGCMLMRGHEYVRKVLLNRLVVVAAVFAAISVVLWAASSFLLL
                     PPSHVERLSWVHNVPPIYSYADVYYNVQDAHVLGMTVWRNSSIFNEPPCAAAFYGMVLA
                     IDLYIGKKVHWGADLCIIGALVTSLSTGGALYVLVLLVPLLWKAMLHVQRRGLRYALLS
                     VAALVSIAAVVVGTHIVISKMATSYSGQTHLLDFTEGFRIWWQRPLTGFGFDSDEYIWT
                     HYMSAYRDGMGYTSGLLFLLIHGGLVLALYMIVPFVLLVLGSHDWHVWYFAGFVMIVFI
                     TAPVQNCAFLLLVAVYGYASFIWRYSRRMSNLPLVARTPLTHEGSEGHA"
     gene            complement(8338..9231)
                     /locus_tag="CNCMI4602_1337"
     CDS             complement(8338..9231)
                     /locus_tag="CNCMI4602_1337"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AYN24180.1"
                     /translation="MRCAGIVTYNPDLERLRANLNNTVSQVDAVFIFDNGSENVADIRS
                     LADSEYTTMKVEADPNNGGIAFALNRLLDAAKEHGFKHMLLLDQDSVPTEGMCDELERH
                     LTGSVALVCPFILDRNRMTEEEWQAQKMPPLERLTHAAKHGAITSGSLVDVAAALAVGG
                     FDDNLFIDYVDFDFNERLILNGYEIVKDNTVYLLHEKGKSERTWLKVPRRTSAGVQWQP
                     LYKLGYSPMRCYYQSRNRIVYWKKYHRHTGGEGLTEIPLLMALSLLFENHRIAKLRAYS
                     RGIHDGMKMKVVEYRK"
     gene            complement(9244..10008)
                     /locus_tag="CNCMI4602_1338"
     CDS             complement(9244..10008)
                     /locus_tag="CNCMI4602_1338"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AYN24181.1"
                     /translation="MPVSSETIMYIVSHKDFDAPQLPGYVPILAGAVKNHASIEVKDNT
                     GDNISAKNPGFCELTAQYWVWKNRDDRDANIGFVHYRRYFYTDHRKTQIVPAEQFSRDL
                     EQYDVILPEPWVLKKTIGNQFAQFHNMDDLEVVRSIIQEQCPEYIPALDEMLKNHSLYA
                     YNMFVMSGERFDAYMTWLFRILDEAERRIDVSKYDAYNQRLFGFLSERLLNVWVNARKL
                     QVKEYPVYMPEQSAWKESLRTRVKSLLFEHKL"
     gene            complement(10010..10897)
                     /locus_tag="CNCMI4602_1339"
     CDS             complement(10010..10897)
                     /locus_tag="CNCMI4602_1339"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2_Glycos_transf_2|GT2"
                     /protein_id="AYN24182.1"
                     /translation="MDGAISVIVPTLNAGRQIDTLLARLEQQTRKPDEIIVVDSSSEDI
                     TQEMVQRHLEVKLIVIDRKDFNHGGTRDAMLRESTGDFVLFLTQDAMPSDNDYIAKIVA
                     PFTDDEVALVYGRQLPRKAARPAEVLVRKYSYGDESMVVTKADIPSLGIKAFHSTDVCA
                     AYRRTAYLEVGGFETDVLTNEDMFIAARFLHAGWKIVYEANATVIHSHNFTFKQQYKRN
                     YIQGVEIAKHKDILGDASLSGEGLSLVKYTLGGLLRQGHVIEAMRFTVDCGFRLIGNRN
                     GQRAAAKSHGRKER"
     gene            complement(11050..11982)
                     /locus_tag="CNCMI4602_1340"
     CDS             complement(11050..11982)
                     /locus_tag="CNCMI4602_1340"
                     /codon_start=1
                     /transl_table=11
                     /product="GT32"
                     /protein_id="AYN24183.1"
                     /translation="MGKIRNVRSKLARFGSEFDSSRQLFGSRIAALGYINHVTGKLGEV
                     YFKQLGDLTWQKLMKAIPCHPQKLSDNLQHAECAPIWVMWWQGLDVNTPAIVKACIRSI
                     RAHAGSHPVHIVTKDNVTAFADIDPKVLKELDRGNISLALFSDIVRFALLYTHGGAWID
                     STVYVTEIIPEKVFSAPFYSIPVHRRNPPRNWAAYFIAGVKGNPLFKYMYDALDKLACA
                     GKGVPEYFMIDVMLYEAYVHYPEFQRMIDSVPENNLGRFELSEQLQSTDEKPRLSAGNY
                     INKLTYKINYPTTVNGKETIYQRLLDGEF"
     gene            complement(11997..13244)
                     /locus_tag="CNCMI4602_1341"
     CDS             complement(11997..13244)
                     /locus_tag="CNCMI4602_1341"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate glucose dehydrogenase"
                     /protein_id="AYN24184.1"
                     /translation="MKIAVAGTGYVGLSVALLLSQHNEVHALDIVPEKVELLNNCESPI
                     VDTEITRFLDGKRDGTMDLDFQATLDPAVAYTGADYAVVATPTNYDSKKNYFDTSSVEA
                     AIKAIREYDPDCWIVIKSTIPVGYTAQLREKTGDKKIIFSPEFLREGHALYDNLHPSRI
                     VVGAPTDDAQAVKAAQTFAMLLAEGAAPEENDRLSADGSTGIPELVCGTTEAEAIKLFA
                     NTFLALRVAYFNELDTYAESRGLNTAEIIRGVCLDPRIGTHYNNPSFGYGGYCLPKDSK
                     QLLANYADVPQNLIGAIVESNRTRKDFIADQILEKIDGVKNPVVGIYRLTMKSGSDNFR
                     QSSIQGIMKRIKAKGIDVLVYEPTLGAAEFFGSEVTHDLDAFKKCCDIIVANRWNESDL
                     ADAADKVYTRDLFRRD"
     gene            complement(13343..14878)
                     /locus_tag="CNCMI4602_1342"
     CDS             complement(13343..14878)
                     /locus_tag="CNCMI4602_1342"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|H8E4X1|9.B.18.1.2"
                     /protein_id="AYN24185.1"
                     /translation="MAQAKSDAQLAEEVDVRSSKVLRWRFVMNLALMLFDALVYFATSM
                     IVLVVTHGTNLMTTRLQLNLPIDPLLYSAVCAAIYVYCMHVAGVYHRHVFADGYQLNML
                     LLKGGFIAWVAICALDFIIQCNVFFYMVCFTSLIVWIAEMIVRFIARVILTSKHRKGEY
                     SYPTVIVGSPEGIGQILKFLNQRKQLNYKPIRLCPIRVNSETGLIEGDPDIELLNQEVH
                     ESWNSPIDVIEYGPQLAGRIAETGAQTVMVTDVLDRFSDNLSTFALQMESVNLEIALIT
                     SALDIAEHATQMRNIQGMSIMTISLPQYSPSAQIKKRVFDLVVSTIAIILSSPIMLIVA
                     LAIKIEDGGPVFYKQERIGLRGKPFYIHKFRSMRVDADAHLKEVLAAQGQELGARYKLK
                     HDPRITKVGNFIRKTSLDELPQFFDVFTGSMSVVGPRPQRSFEVAEYDPVYATRLLVKP
                     GITGPWQVSGRNDLNEEESRQLDVSYVQDWSVMGDVVYILRTIGTVLHPSGAY"
ORIGIN
        1 tcaatcgtcg atggtgctga ggaaccgatg gtcggcaacg tcgagcaggt gcttgccgta
       61 cgggctcttg ccataccgtt cggcagcagc gagcagttgg tcacggtcga tccagtggtt
      121 ctcgtaggca atctcctcga tcaccgagat cggcaacccc tgagcgtgct ccactgtgcg
      181 cacgaattcg ccggcttcga atagcgagtc catggtgccg gtgtcgagcc acgcatagcc
      241 tcgaccgagc gtctgcacat gcaatgaacc atcctcaagg tacatacggt tcaggtcggt
      301 gatctccagt tcgcctcgag gagatggttt gacttgcttg gcgaagtcga ccacgcggtt
      361 gtcatagaaa tacaggccgg tcaccgcata gttcgatgca gggtgctcgg gtttctccac
      421 aatgctcact gccttgtggt ccttgtcaaa ctcaacgaca ccgtaccgtt ccgggtcatc
      481 cacgtagtag ccgaacaccg tagcgccatg ttcgagcgcg gctgccttgc gcagtaggcg
      541 gcccaaaccg ttgccgtaga agatgttgtc gccgagcacg agggcacatg catcgtcacc
      601 gatgaagtcc tcaccgagca ggaaggcctg tgcgaggccg tccgggctcg gttgcacctt
      661 gtactgcaac gagatgccat aacgggagcc atcgccaagc aggcgctcga aattcggcaa
      721 atccttcggc gtcgagataa tcaggatgtc tcggattccc gccatcatca gcgcactcaa
      781 tgggtagaag atcatcggct tgtcgtacac cggcaacaac tgcttggagg tgactgttgt
      841 caacgggtac aggcgagtgc cggaaccgcc ggcaagaata atgcctttca tctgggaccc
      901 atcctcactt cttcaattca gcttcgacgt atgccttgag gctgtcgtgc caatcggtgg
      961 tctcgtagcc ggtctcctcg atcttggcca agtcgagcgt gctgttcgtc ggccgtgggc
     1021 tcactggagc cttcgcgttc tcgaagtact gctcggtgga gatcggacgc accttgtcgc
     1081 cattgccatt cgtcatggcg aacacctcgg cggcaatctg tgcccagctc accacgtcac
     1141 cggaaccagt gagatcgtac gtgccatacg gatcctcatc atcaagcaga tggaaaatgg
     1201 cagctgccat gtcatcggtg aacgtcagac ggccatattg gtcatccact accgtcacct
     1261 cattcaatgc atcattcgga tcagacacct tgttcgagag catcatcatc gtcttgacga
     1321 agttgtgccc gttgccaatc acccaactcg agcgcacgat atagtgcttc ggggcgttgg
     1381 tcacagcgat gtcgccggcc gccttcgtct gtccatatac gccgagcggc gagaacgcct
     1441 catcttcgtc atgcaccttg gccgtaccat cgaacacata gtcggagctc acatgcacca
     1501 aggtcaggtt atgctcggcg gccactttcg tcagcaatgc cgggccaagg gcattggcct
     1561 tccatgccgt cacacgcccc tcgggtgtct cggccttatc caccgccgtg aatgccccgg
     1621 cattgatgat cgtgccgtac aggctccagt catagccctc ataggcggca ggatccgaga
     1681 aatcgaattc gtcgatgtcg gtgaattcga atcctttgag atcatgctct tcaacatact
     1741 tgcgaatcga ctgcccgagc tgtccgttcg caccggtgac caatgtacgc ttcggatcca
     1801 ttggcttggc atccttgagc atcgggtgat gcaggtccgc tttggagagt tcggcctgct
     1861 caagcggaat cggccattgg atgttgagtt ccgggtcggc caggttcacg aaggtgtagg
     1921 tcttcttctg ctcgagagac caatgcgcgt tcaccaaata cgtgtatgca gtgccatctt
     1981 cgagcgcctg gaagctgttg cccactccgc gtggcacata gattgccttc gacggatcga
     2041 ggcgcgtcgt atatacctgc ccgaaggtgt cacctggacg cagatccacc caagcaccga
     2101 agatctcgcc tgtggccagc gagatgaact tgtcccacgg ctcggcgtgg atgccacggg
     2161 tcacaccgcg tttcgcattg aaactgacat tgttctgtac cggctggaag tccttcggca
     2221 agccgagtgc cgtcatcttc gccttctgcc agttctcctt gaaccagccg cggttatcgc
     2281 cgtgcaccgg caaatcgaag acaagcaggc ctggaatgtt cgtctctgtg accttgagtt
     2341 ccttttcgaa ttccatattt cacgctttcc acatcactgt tgcatgtctg tacatgatga
     2401 atccgccgta gttgcggcgg attcaaagca ttcactggcc ttgggccttg tatttggcct
     2461 cggtggcggc tttcgccggc tcccaccact gcggattgtc gatgtaccat tgcacggtct
     2521 ggcgcaggcc tgactcgaag tcggtgtgcg ttgggcgcca acccaactcg gtctgcaact
     2581 tcgtcgaatc gatggcatac ctgcggtcgt ggcccggacg gtccttcacc caatcgaagg
     2641 cgtcctccgg ctgacccatc acccgcagaa tgtcgtgcag cacggtcagg ttgttgcgct
     2701 caccattggc gccaatgagg taggtttcgc ccaaacggcc agtggtcaga atcgtccaca
     2761 ccgcgctcga atgatcttcg gtgtgaatcc agtcacgtac attgaggcca tcgccataaa
     2821 gcttcgggcg aatgccttca atgatgttcg tgatctggcg cgggatgaac ttctccacat
     2881 gctggtacgg gccatagttg ttcgagcagt tcgagatcgt ggtgcgcaac ccataggtgc
     2941 ggtgccatgc gcgcacgagc atgtcggagc tcgccttcgt cgatgaatac gggcttgacg
     3001 gatggtacgg cgtttcctcg gtgaacttcg ctggatcatc cagagccagg tcgccataca
     3061 cctcgtcggt gctgatgtga tggaaccgca catcatactt gcgcgccgct tcaagcaggc
     3121 ggaacgtacc ctcgacgttc gtcttcacga acggctccgg attcgcgatt gaattgtcgt
     3181 tatgcgactc ggctgcataa tgcacgatgg catcatgacc tggcaccagt tcgtcaagca
     3241 gctgtgcatc gcagatgttg ccatgcacga actcgacacg atcgccgaga atcggcttga
     3301 tgttctcgag gttgcccgca tatgtaagtg catcaagtac cgtaatgtgc acatccgggt
     3361 gattgttata cacgtaatgc acgaaattcg agccgatgaa accgcagccg ccggtcacaa
     3421 tgatgttctt cggaatgaac gcttctgtca tggctactac tgtagcaatg agacaccatt
     3481 cattcatcca atacccattt tttctctata gacacatgcc ttccatacgc cgcatatcga
     3541 atcctaggca atggaattcg agcatcagga gattccgccg atacgcacga caaactacta
     3601 tgagtatgtc ggttggtttt aatcgaatac ccatggagca gtctcgatga gcaaatccct
     3661 agtcaagaac tcattcttca atgttgtcta tcaattgttg aatgtgctgt ttccactcat
     3721 cacagcaccg tacgtggctc gcgtgctgct accggaaaat gtcggcaaag tggcagtggc
     3781 acagaactgg gcacaatact ttatcgtgtt cgcggcactc ggccttccca cctacgcggt
     3841 gcgggaaacc gcccaacatc ggaagaacaa gcaggaactc gacaaggctt tcagcgagct
     3901 gctgatgatc aacaccatct ccaccacgat cagcgtcatc gcctacataa tcatgctgtt
     3961 caccttcgga accttccgcg agaacatcgc actgtatttg gccgttggcg ctggcatcct
     4021 gttcaactac atcaccatcg actggctcta ccaaggcatt gaggactacg gctacattgc
     4081 catgcgcagc ttcatcgtca aactgctctc gctcatcgca ttgctggtgt tggtgcggaa
     4141 caggaacgat ttcgtcatct acgcgttgat ctccacctgt gcggtcggtg cgaacaacat
     4201 actcaacgtg ctgcacctcc ccaagatcgg tgtgcacctg acattcagga atctcacatt
     4261 caaacagcac gtcaagccaa tcatgatttt cttcgccaac tcgatttcgg taagactcta
     4321 cacactccta aacgtgacat ttcttggcat attctccaca gatgacgctg tcgcgtttta
     4381 ttccaattcc gataaagtcg tccgcattat cattacagta atcgccggca ttgggaatgt
     4441 cctgctccct cgcttgagca tttatgccaa acgtggagaa aaaaaacggt gctcaaatat
     4501 cgtgtcttca gtatttaaca tccttttctt catattcgta cctactggta ttgggctgat
     4561 acttatgtcg aaagaaatag tactcatatt attcgggtct gcttatgccc catctattct
     4621 aacgttgcaa ataatgtcac ttctcatcta tacactagga ttcaataatc tttttgtctc
     4681 tatcaatctt gctttcgcca aagataaaga atatcttata tgctctatct ctggagctct
     4741 ttcctgctta gttttaaatt atgtgctcat ccccgtgctc caacaaaacg gaactgctat
     4801 tgcctctgtg ctgagcgaag gcatagtcac cggagtcgct attttcttct cagcccaaac
     4861 aatcaaacta cattttgaca aaaaaatcat tgccaagact atcttggcct ccattctcat
     4921 ggctgtcgta gtctattgtt tcaaattact gattagtaat ctcattttgt gtcttatcgc
     4981 cagcgtcgga tttggatcgc taacttacat aataatttcc tggctgctga agaaccccgt
     5041 gctcttccag atcatcaacc tcacactgaa atgccgtaaa taaccgagcc taccgtgtgt
     5101 agttacgtag taccttcaat gacttcacgt aattactcag cgtgaagatc atcttcctat
     5161 gctcatcttt cctcatcatc tggtcagtgg agacatcttt catatgatcg acccggatgt
     5221 gcttgtcgta ccacatcgtc aggtgccggg ctcggcatcg catcaacaga atgtcttcct
     5281 caaaatagag gaaggttccg gagtcgaatg cgcagctgaa ctgcttgacg aatgccggtg
     5341 tgaatacaaa gcacgcacca tggagcttgt aaggtctgcc ggtaatggca tctgccgtgg
     5401 cattgacatg ctcaggcaat gcattggcat cggaatgcgt gagttttgcc ttgagtcggg
     5461 tcagcgggcc gaacaggtga aggtgcagca gaagcaggat taccttgaag cgccatatct
     5521 gacgatccac ctccgcgcgg gtgtcgatga tcgaatagat cgggttcgag tgcgaaccgt
     5581 tcttgcctga cacaatgtcc ggaccaatca cccccacatc aggatgcgcc ttgaaatcct
     5641 cctcgcacag ctccatgaaa tcatcgcagg caatggtggc acgttgttca tcgtgacgat
     5701 gaaatcgcaa tgtaggcgat tccgcgcata ttcatacccg gtattgttac cgccggcgaa
     5761 cccttcattc actgcgttat gcagcaatgt cacggtggaa tcagcaccat acttgcgttg
     5821 cagctcttcg tagctaccat tggcagagtt attgtccacc accactatgg cggcttgagg
     5881 gcaatgcgtt ttgatggatt cgatgcaacc tgccgtggca tctcccgcaa gataatgcag
     5941 caccacgaat ccgaatttcg cagcagtcat ggatctcctt cagtggtatc agcccttggc
     6001 acgcaaggca tgcaccagaa gcgaacgcaa cacggcattt cgcatcatcg tgtatttgaa
     6061 tcgaattgca gggctcaatg cggaaacatc aatctgatcc aatcgtggcc ccaatgcctg
     6121 tgcacgagtc cgagccgcct gcttggcgga ttgggggtcg gcgagcatga tgaccgcctt
     6181 cagttcctgg aaatacaggt tcacctgctc ctcgttccaa gcccaaagca acgtcgcatc
     6241 tccatcgaga tggtactcat gcatgagccg ggcacgctcg tcaatggccg gatggtatag
     6301 acccttggca ttgtttttgt aggtgcgtgc actcaatccg cccatacggt agtaataccc
     6361 tgcataatcc acatacacaa tctcgtgtgc caattggaaa gcctgcatat tgaacagatt
     6421 gtcttgcatg cgaggcaatg ctgcgtcgaa ctgcagtcca tgctcctgca ggaaagcgtg
     6481 cctgtacagt ttgccccacg gcactccaat ggccgtgacg aatcctttgg gctgtgcata
     6541 tgaaccatcc atcaattgac ggaacagttc caccttctcg tcagcggcac gggccgtgaa
     6601 ggattcaggg aagaaatgct gcctggcgga atgctggtca tcgaatgcca cgcaggtgct
     6661 catgacgata tccggattac gttcggtggc tttcagcagg gtttcgagat agtcaggagc
     6721 cacataatca tccggatcga tgaagcacac ccattcgcct ttggcatggg acagtccatt
     6781 gttacgggcc acggatacgc cggaattgtt ctgatcaatg agctgaacac gaccatcacg
     6841 ctgctcgtag ccctcgagaa tggaggcgga ggcatcggtg gaaccgtcat tgacggccag
     6901 cacctcgaaa tcctcgaaac tctggtcgag gagcgaatcc atgcatgcgc ctacgtattc
     6961 ttcttggttg tataccggaa tgattaccga gatctcaggc atgcccctca ctcccctcat
     7021 gggtcaatgg cgtacgggcc accaatggca gattgctcat acgtctactg taacgccaaa
     7081 tgaagctggc atagccgtac acggccacga gcaacaggaa cgcgcaattc tgtaccggcg
     7141 cggtgatgaa cacgatcatc acgaatccgg cgaaatacca cacgtgccaa tcatgggaac
     7201 ctagcaccag cagcacaaat ggcacaatca tataaagcgc cagcactaat ccaccatgga
     7261 tgagcaggaa cagcaaacca ctggtatacc ccatgccatc gcggtaggca ctcatataat
     7321 gcgtccaaat gtactcgtcg gaatcgaatc cgaatcccgt cagcgggcgc tgccaccaaa
     7381 tgcggaagcc ttcagtgaaa tcgagcaagt gggtttggcc ggaataggac gtggccattt
     7441 tggagattac gatgtgcgtg cctacgacca cggcagcgat ggacacaaga gccgcaacac
     7501 tgagcaacgc atagcgcaat cctctgcgct gtacgtgaag catggccttc catagcaacg
     7561 gcaccaacag taccaatacg tagagcgcgc ccccagtgga cagggatgtg acaagtgcgc
     7621 cgataatgca gagatcggcg ccccagtgca ctttcttgcc tatatacaag tcgatggcga
     7681 gtaccatacc gtaaaaagcc gcagcgcagg gtggttcatt gaaaatcgac gagtttctcc
     7741 acaccgtcat gcccagtaca tgagcatcct gcacgttgta gtacacatcc gcgtacgaat
     7801 agatcggtgg tacattatgc acccaactca atcgttcgac gtgcgagggt ggcagcagaa
     7861 gaaaggagct ggccgcccat agcaccactg atatagcggc gaacaccgcc gccaccacca
     7921 ccaaacggtt gagcagcacc ttacgcacat attcgtggcc acgcatgagc atgcacccca
     7981 tcaatgcagg cacgatgagc gcatattgga accagatgcc gtccgtgtac cctgatcccc
     8041 catgagagac cgccattacc aactgcccaa tgacggtggc cagcacgagc acgagcatcg
     8101 cagaccccac gcgatgcaga cggtgccgcg cctcgcgcca acaacacagc atgtacgcaa
     8161 ggcacacggc aagcaacacg aagcacgaga gcgagaaatt atcatctgca agtgcccaca
     8221 acgaattcga gttgtagatg atggcgagaa caagcgcgta atccatcaac gtgttcacca
     8281 cctcatgaat tcgcggggta tatgccgcct gccgttgaac ggtttcggtg gtgtctgtca
     8341 cttcctgtac tccacaacct tcatcttcat gccatcgtga atgccccgcg aataggcgcg
     8401 tagttttgca atgcgatggt tctcgaacag caatgacaat gccatgagca acggaatttc
     8461 cgtaagcccc tcgccaccgg tgtgcctatg gtatttcttc caatacacaa tgcggttgcg
     8521 tgactggtag tagcaacgca ttggtgagta gccgagctta tacagcggct gccattgcac
     8581 acccgccgac gtcctgcgag gcaccttcag ccaggtacgc tcggatttgc ccttctcatg
     8641 caacagatag acggtgttgt ctttgacaat ctcatagccg ttcaatatca ggcgctcgtt
     8701 gaaatcgaag tccacgtaat cgatgaacaa gttgtcatcg aagccaccaa ctgctaaggc
     8761 cgctgcaaca tcgaccaagg aacccgaagt gatcgcacca tgcttggcag catgggtcag
     8821 ccgctccaac ggtggcatct tctgcgcttg ccactcctcc tcggtcatgc ggttgcggtc
     8881 gagtatgaat gggcatacca acgccacgga accggtgaga tgtcgttcca actcgtcgca
     8941 catgccttcc gtgggcacac tgtcttgatc gagcagcagc atatgcttga acccgtgctc
     9001 cttggcggca tccaacaacc tgttcaaggc aaaggctatg cccccgttat tcgggtcggc
     9061 ttcgaccttc atggtcgtat attccgagtc cgcaagcgag cggatatccg cgacgttctc
     9121 cgatccattg tcgaagatga acaccgcatc cacttggctg acggtgttat tcaggttcgc
     9181 acgcaaccgt tcgagatcgg gattataggt gactatccct gcgcaacgca tacggtaccc
     9241 tctctacagc ttgtgttcga acagcagcga ttttacacgc gtgcgcagac tctccttcca
     9301 cgcactttgc tcgggcatgt ataccgggta ttccttgacc tgcagcttgc gcgcgttcac
     9361 ccatacgttg agcagacgct cggaaaggaa tccgaagagc cgctgattgt atgcgtcata
     9421 cttcgacaca tcgatgcgac gctcagcctc gtcgagaata cggaacagcc aggtcatata
     9481 tgcatcgaac cgctcacccg acatgacgaa catgttgtag gcatatagcg aatggttttt
     9541 cagcatctca tccaatgccg gtatgtactc cgggcattgc tcttgtatga tcgaccgcac
     9601 gacttccaag tcatccatgt tgtggaactg ggcgaactgg ttccctatgg tcttcttcaa
     9661 cacccacggc tcagggagga tcacatcgta ttgttcaaga tcgcgtgaga attgttcggc
     9721 tggaacaatc tgcgtttttc ggtggtcggt atagaaataa cgacgatagt gcacgaagcc
     9781 gatgttcgca tcacggtcat cacggttctt ccacacccaa tactgcgcgg tgagctcgca
     9841 gaaccccgga ttcttggcag agatgttatc gccagtattg tcttttacct caatggaggc
     9901 atggttcttg accgcccctg caagaatggg aacataccca ggaagctggg gtgcatcaaa
     9961 atccttgtgg cttacgatat acatgatcgt ctcggaggaa acaggcactt tatctctcct
    10021 tcctgccatg agatttggca gcagccctct gaccattgcg attgccgatg aggcggaatc
    10081 cgcaatcaac ggtgaatcgc attgcttcaa tcacatgccc ttgcctgagc aaaccgccaa
    10141 gcgtgtactt gaccaaggag agtccttcgc cagacaatga cgcatcgcca agaatgtctt
    10201 tgtgcttggc aatctccacg ccctgaatgt agttgcgctt gtactgctgc ttgaaggtga
    10261 agttgtggga atgaatcacc gtggcgttcg cttcatacac gatcttccac cctgcatgca
    10321 ggaagcgcgc agcaatgaac atatcttcgt tggtcagcac atccgtttcg aaaccaccga
    10381 cttccagata tgccgtacga cgataggccg cacatacatc ggtagagtgg aaggccttga
    10441 tgcccaagct tggaatatcg gccttggtga ccaccatgga ttcatcgcca tacgagtact
    10501 tacgaacgag tacctctgcc gggcgtgcgg ccttgcgtgg aagttgacgt ccatatacca
    10561 gcgccacttc atcgtctgtg aatggggcca caatcttcgc aatatagtca ttgtcacttg
    10621 gcatggcgtc ctgcgtgagg aacagcacga aatcaccagt gctctcgcga agcatcgcat
    10681 cgcgcgtacc accgtgattg aaatccttgc ggtcgatgac gatcagtttc acctcgagat
    10741 ggcgctggac catctcctgc gtgatgtctt cagacgagga gtccacaaca atgatctcat
    10801 cgggtttacg cgtctgctgc tccaatcgtg caagcaatgt gtcgatttgc ctgcccgcat
    10861 tcagcgtagg tacaataacc gagattgcgc catccaccaa ctcgtttcct cccacgtctc
    10921 atacgccatt acattattca ttccgagctc cgggaaccta caggaatggt aacagcatac
    10981 gctgattacg ttcagctcag ctcaaatcgc agtggtattg cgtaggtttc atacatgaca
    11041 ccttccacct tagaattcac catcaagaag acgttggtag atggtttcct taccattgac
    11101 cgtggttgga taattgattt tgtatgtcaa tttattgatg taattacccg cagataaccg
    11161 tggcttttca tcagtcgact gcagttgctc gctgagttcg aacctgccaa gattattctc
    11221 tggtacggag tcaatcatcc gctggaattc cgggtaatgc acatatgcct catatagcat
    11281 cacatctatc atgaaatact cgggcacacc tttccccgcg caagcaagtt tgtccagagc
    11341 gtcgtacatg tatttgaaaa gagggttgcc tttgactccg gcaatgaaat aagctgccca
    11401 attcctagga ggattcctcc gatgcaccgg aatcgaatag aatggcgcgg agaagacctt
    11461 ctcagggatt atttccgtga catacaccgt tgaatcaatc caagcacccc cgtgcgtgta
    11521 cagtaaagca aaccgaacaa tatccgagaa taatgcaagg ctgatattcc ccctgtccag
    11581 ctctttcaag acctttggat caatgtcggc gaatgcagtc acattgtctt tcgtgactat
    11641 atgcaccgga tgactaccag cgtgagctcg aatgcttcgt atacaggctt ttacgattgc
    11701 tggggtgtta acgtccaaac cttgccacca cataacccaa ataggtgcgc actcagcatg
    11761 ctgtaagttg tcggacagct tctgtggatg gcatggtatc gccttcatga gcttttgcca
    11821 tgtaaggtcg ccaagctgtt tgaagtacac ttctcccaat ttacctgtta catggttgat
    11881 gtaccccaat gcagcgatgc gactcccaaa tagctggcgc gagctatcga actcactccc
    11941 aaaacgcgcc aatttgctcc tcacattgcg aattttgccc atgacgtccc ttgaaatcaa
    12001 tcgcggcgga agagatcgcg cgtatacacc ttgtcggcgg catcggcgag atccgactca
    12061 ttccatcggt tcgcgacgat gatgtcgcag cacttcttga aggcatcgag atcgtgcgtg
    12121 acctcactgc caaagaactc ggcagcgccg agcgtcggct catacacgag cacgtcaatg
    12181 cccttggcct tgatacgctt catgatgcct tggatgctcg actggcggaa gttgtcggaa
    12241 cccgacttca tcgtgagacg atagatgccc acaaccggat tcttcacacc gtcgatcttc
    12301 tcgaggattt gatcggcgat gaaatccttg cgcgtgcggt tcgattcgac aatcgccccg
    12361 atgagattct gcggcacatc ggcgtaattg gccagcagct gcttcgaatc cttcggcaga
    12421 cagtagccgc catacccgaa cgatgggttg ttgtaatgcg tgccgatgcg cgggtcgagg
    12481 cacacgccgc gaatgatctc ggcagtgttc agaccacgcg actctgcata ggtgtcgagc
    12541 tcattgaaat aggccactcg cagtgcgagg aaggtgttgg cgaacaactt gatcgcctcg
    12601 gcctcagtcg tgccgcacac cagttccgga atgcctgtgg aaccatcggc gctgagtcga
    12661 tcgttttcct ctggagccgc accttccgcc agaagcatcg cgaacgtctg cgctgccttc
    12721 accgcctggg catcatcggt aggggctccc acaacaatgc gggaaggatg gaggttgtcg
    12781 tagagcgcat ggccctctcg caggaactcg ggagagaaga taatcttctt atcccccgtc
    12841 ttctcacgca actgagccgt atagcctacc ggaatcgtcg acttgatgac aatccagcaa
    12901 tccgggtcat attcacgaat cgccttgata gcggcctcga cgctggaggt atcgaagtag
    12961 ttcttcttcg agtcatagtt cgtcggagtt gccaccaccg cgtaatcggc accggtatag
    13021 gcgacagccg ggtcgagcgt ggcctggaag tcgagatcca tcgtgccatc gcgcttgcca
    13081 tcgaggaaac gcgtgatctc ggtatcgaca atcgggctct cgcaattgtt gagcagttcg
    13141 accttctcgg gaacgatgtc gagcgcatgc acctcattat gctgcgacag caacaatgca
    13201 accgacagtc ccacgtatcc cgtaccggca acagcaatct tcataacaac tccaatactg
    13261 tatgcgctag ccacatgact aacaatcaac aatcatactg aagattggaa catgatcata
    13321 gtattttcat cttcatcagc gtttagtacg ctccactcgg atgcaatacc gtgccaatcg
    13381 tgcgcagaat gtacacgaca tcacccatga cgctccagtc ctgcacataa ctcacatcga
    13441 gctgacggct ctcctcctca ttgaggtcat tgcggccaga cacctgccag gggccagtga
    13501 tgccgggctt gaccagcagg cgcgtcgcat agaccgggtc gtattcggcc acttcgaacg
    13561 aacgctgtgg gcgcggcccc accacactca tcgaacccgt gaacacgtcg aagaactgcg
    13621 gtagctcgtc gaggctcgtc ttgcgaatga agttacccac cttcgtgatg cgcgggtcgt
    13681 gcttgagctt gtaccgggct cccaattcct gcccttgcgc ggccaacacc tctttcaggt
    13741 gtgcgtcggc gtccacacgc atcgaacgga acttatggat gtagaacggc ttgccacgca
    13801 agccgatacg ctcctgcttg tagaacaccg gaccgccgtc ttcaatcttg atcgccaacg
    13861 ccacaatcag catgatcggc gagctcagaa tgattgcaat cgtcgacacc acgagatcga
    13921 acacgcgttt cttaatctgt gcggaggggg agtactgagg cagactgatc gtcatgatgc
    13981 tcataccctg aatgttgcgc atctgcgtgg catgttcggc aatgtcaagc gcagaggtga
    14041 tcagcgcgat ctcaagattc acagattcca tctgcaaagc aaaagtgctc agattgtcgg
    14101 agaagcggtc gagcacatcg gtgaccatca ccgtctgtgc gccggtttcc gcaatccgtc
    14161 cagccagctg gggtccatat tcaatcacat caatcgggga attccatgac tcgtgcacct
    14221 cttgattgag taattcgatg tcagggtctc cctcaatgag tcccgtctca ctattgaccc
    14281 gaatcgggca caggcgaatc ggtttgtaat tgagctgctt gcgctggttg aggaacttga
    14341 gaatctgccc aatgccctcg ggggaaccga cgatgacagt cggatacgaa tactccccct
    14401 tgcgatgctt gctggtgagg ataacgcgcg cgatgaagcg aacgatcatt tccgcaatcc
    14461 acacaatcaa cgaggtgaag cagaccatgt agaagaacac gttgcactga ataatgaagt
    14521 cgagcgcgca aatcgccacc cacgcaatga aaccgccctt gagcaacagc atattgagct
    14581 ggtacccgtc ggcgaacaca tgtcgatggt atacccctgc cacatgcatg caatagacgt
    14641 agattgccgc acatacagcc gaatagagca gtggatctat aggaagattc aactgcaggc
    14701 gagtcgtcat gaggtttgtg ccatgtgtca cgacgagcac aatcatgctg gtagcgaagt
    14761 aaaccaacgc atcgaacagc atgagtgcca gattcatgac aaaacgccag cgcagtacct
    14821 tgctggaacg gacgtccact tcctcagcga gctgagcatc gcttttcgcc tgtgccat
//
