LOCUS       AB812066               14841 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: E110-69.
ACCESSION   AB812066
VERSION     AB812066.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 14841)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..14841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E110-69"
                     /db_xref="taxon:562"
                     /note="serogroup:O160"
     gene            <1..867
                     /gene="wcaM"
     CDS             <1..867
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01806.1"
                     /translation="IVANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGK
                     INWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNI
                     TPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNI
                     QLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIRVMQESSV
                     GPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINF
                     RLPERRE"
     gene            1025..2020
                     /gene="gne"
     CDS             1025..2020
                     /gene="gne"
                     /note="O160_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ01807.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQI
                     GDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNI
                     IFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTV
                     IFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYN
                     YVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRV
                     KKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2263..3156
                     /gene="galF"
     CDS             2263..3156
                     /gene="galF"
                     /note="O160_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01808.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIV
                     AAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQ
                     GEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVL
                     AKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIW
                     PELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNL
                     KEGDKFRMSVKKLLEI"
     gene            3506..4753
                     /gene="wzx"
     CDS             3506..4753
                     /gene="wzx"
                     /note="O160_03"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="BAQ01809.1"
                     /translation="MSLKKNVIYLVLMQAVNYIAPLVLVPYLTRILGVEKYGVLGLAIT
                     VSQYLILLTDFGFNFTASRKIAQFKDSKVRVSQIFWTIISAKFLMMIVSFGLIVPFVVF
                     SEKLNPLKWEIFLVSLSVVASVIIPSWLFQGLEKVTVFSGINIFSKILIVPLVFIFVKS
                     KEDLLIACLLQGGVQVFSGIISILYVKYNKIISFKVVRPKLIFIYLKESLSVFLGNLSI
                     SLYTLSTPLVLALMGTTYQVGLYSATDRIRGAAIGIFIVVGYAIFPRVSYLFKKNPLEA
                     NVLLKKIIFIFSILGCLGGILVYSIADEIVLVAFGNQYLDSAILLKIMAPMFLLIPLSI
                     IMANYLLLPNGFKKEYAKNSVIVCLLHMIYVFPLCKYYGAVGGSYSILISEIISFILLI
                     FWTIKNNLLKKVFYAR"
     CDS             4743..5744
                     /note="O160_04"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01810.1"
                     /translation="MQDNKSIKLSVIIPCWNCSKYITKTLDSIKYSYYMSRKPILEIIL
                     VDDGSTDLTSKIIKAYDFGTRAVNVKYHFQNNAGPSKARNQGIKLAQGRYVTFLDSDDI
                     WSPDYLRIIESIMDKYDSEIIEFNAVRFIEENANLKIHNNYTLVDEEYHGPINERILSE
                     VFIKSEWYVWARVYKKKLLEGESFNENITHHEDAEFLPRIYLTAKSISRITSQLIYYRL
                     TQNSITTKPKISSIKDLTLVCELYYENINGPNDKYYKAAMINCLWGLKRLILDKGEFSF
                     IKKSRILYYCHIARNSSQLFNMLSWKKRLFIYLPRTYIFLNSLKYKLSKRGA"
     CDS             5744..6676
                     /note="O160_05"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01811.1"
                     /translation="MEISIVIPLYNKENYIKRTLLSIIDTFGRVYNEKEYEIVIVDDGS
                     KDKSVSVVESINSNVVKLYKQENGGPSKARNNGVNFSDGKYVIFCDADDIILPNYGDYI
                     KYSIDNYPDHDVFVAFSRVLRNSKENYSIPLFDPVDVSIVDDFFYEWDKRKFFSASSIC
                     IKKHFFIQNDLYFDETISSGEDLLMFYKAAIKTKYVAYSQPAVLYDKTVDSQLSSNPDL
                     KIGAHTYFLIKVYKTENLSVKDKTAINRIIDKQICFVAVDNLLRKKYANSLNIVLFRKR
                     MLLQPKLAIKLIIASISYKLFTKISSLLR"
     gene            6673..7773
                     /gene="wzy"
     CDS             6673..7773
                     /gene="wzy"
                     /note="O160_06"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="BAQ01812.1"
                     /translation="MIIENNKVSSHSYLWVLFQSFIFYYKFMLQSWDGDEQIVNVISII
                     LTLLLLCGTISAFIISNIKEKCIFLIIFIFVALNIIIADNKSVFWMVTAFTFLILFSTL
                     TIKNRIRILVFSFIIAWCFFLPLQIFFSNSYTYIDDRYLRYTFGFLNPNGLGMFLLLLQ
                     TLLYYWIWTSIKATIIVKQMITIILGGSIICIIFLSESRTYILLSFLLLILTVIYGFKK
                     FKFSSRLLFIYLLFVMLLQWLSVKGFENYLIFQDMNAYMSGRVWFSYNLLSQMGEPKFF
                     IGSDISLYQPIDFFFISLLYNNGILASLILLYCNYIFLKKLDNSTKYESILAFIFITVS
                     FTEAVYNIPLLNFFFLLLYKKELRFS"
     CDS             7770..8789
                     /note="O160_07"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01813.1"
                     /translation="MKVDAVILWVDGNDPKWQEEYNKYCKPASRIENGVQRYRDWDTLR
                     YVIRGIEYNLPWIDKIHFVTCGQKPSWMVGYHPKLNFVHHNDIFENDTFLPTFNSSAIE
                     LNLSRIKGLSERFIYFNDDMLVLKNTPLQRFFVNDLPVDFLIEAFPRRGLLYEKIRSNS
                     TWVSMINNCTSLINRVYHKNKYIQDNRNLYYNMNYGRHVIANVLASPFKQFLAFKHYHH
                     PQAYLKKTLQSVEREFPVEFNLTCKSRFREHDNISQALFRYYQLVTGSFYPCYYNDHAC
                     VNVVNKKSASQCIEALHQKRFVCINDEINDDLDDSSILINDIIKELDLILPNKSSFEI"
     CDS             8796..9614
                     /note="O160_08"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01814.1"
                     /translation="MNSFDEFNVLLSLYKNESPDNLDACFQSISTQSLKRFKIILVIDG
                     PISSELNEVVGKWKSLLPIKIINLERNVGLGNALNIGLKYCSCDYVFRMDTDDICHPDR
                     FSIQFSYLRKHPDIDLLGGQIVEFHECIEEPNGMRLVPSKYEEILQYCKLKNPFNHMTV
                     VFKRESVLKVGGYKHHLYMEDYNLWLRMISIGCKVENLDDVIVFARTDVNSLMRRRGWQ
                     YVKSEWKLALLKIKLRINNPIVSLSVFILRSIPRLLPIMLIRRIYAHNRK"
     gene            9763..11169
                     /gene="gnd"
     CDS             9763..11169
                     /gene="gnd"
                     /note="O160_09"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01815.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAE
                     NPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTF
                     FQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVA
                     EDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQTFTEW
                     NNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLIT
                     ESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQL
                     RAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADD
                     YQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDK
                     EGVFHTEWLD"
     gene            12128..13294
                     /gene="ugd"
     CDS             12128..13294
                     /gene="ugd"
                     /note="O160_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01816.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISPI
                     VDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIKDV
                     VEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVIGER
                     SERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLG
                     LNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANR
                     TRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMK
                     EDSFFNSRLERDLATFKQQAEVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            13384..14418
                     /gene="wzz"
     CDS             13384..14418
                     /gene="wzz"
                     /note="O160_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01817.1"
                     /translation="MVIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLVQ
                     LWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAV
                     PKVSDIQTSLIGRYSTAFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETAEGA
                     QKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEA
                     LQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLL
                     DIESLKVDDLDIHAYRYVMKPTLPIRRDSPKNAITLILAVLLGGMVGAGIVLGRNALRN
                     YNAK"
     gene            complement(14515..>14841)
                     /gene="hisI"
     CDS             complement(14515..>14841)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01818.1"
                     /translation="TCHKGTSSCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYA
                     SGTKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLR
                     KRHQ"
ORIGIN
        1 atcgttgcca acatcaccaa ttgtaagttc agcgacttac agggcgacgc cattgaatgg
       61 aacgtggcaa ttaacgacag tgatattttg atctccgacc atgtcatcga gcgcatcaac
      121 tgtactaacg gaaaaatcaa ctggggcatt ggcataggcc ttgcggggag tacttatgat
      181 aataactacc cggaagacca agcagtgaaa aactttgtcg tggcaaatat cacgggatcg
      241 gattgtcggc agttgatcca tgttgaaaat ggcaaacatt ttgttatccg taatatcaaa
      301 gcccgcaata tcacgccgga tttcagtaag aaagcaggca ttgataacgc gacagttgct
      361 atttacggtt gtgacaattt cgtgattgat aatattgaaa tgattaatag cgccgggatg
      421 ttaatcggct atggggtaat taaaggcaaa tatctctcga taccgcaaaa tttccgagtg
      481 aataatattc aactggataa cacccatctt gcttataaat tgcgcggcat ccaaatctcc
      541 gccgggaatg ctgtctcctt tgtggcactg actaacattg agatgaagcg tgcctcgctt
      601 gagctacaca ataaaccgca acatcttttt atgcgaaata tcagagtgat gcaggaatcc
      661 tcagttggac cagcattgag catgaacttt gacatgcgca aagacgttcg cggcgtgttt
      721 atggcgaaaa aagaaacact gctgtctctt gcaaatgttc atgcggtgaa tgaaagaggg
      781 caaagttccg tcgatatcga caggattaat caccatattg ttaatgtgga aaagattaac
      841 tttagattgc cggaacggag ggagtagatt tgcgaccatt cataaactta ctggatataa
      901 tgttctaaaa gattataatc ctcacactaa ctgttaaagc tggggctggg cagcattgac
      961 tactcaggga aggtactcat ctttatcttg tgtttcttac gtaacaacca gactaaagtg
     1021 atttatgaac gataacgttt tgctcatagg agcttccgga ttcgtaggaa cccgactact
     1081 tgaaacggca attgctgact ttaatatcaa gaacctggac aaacagcaga gccactttta
     1141 tccagaaatc acgcagattg gtgatgttcg tgatcaacag gcactcgacc aggcgttagc
     1201 cggttttgac actgttgtac tactggcagc ggaacaccgc gatgacgtca gccctacttc
     1261 tctctattat gatgtcaacg ttcagggtac ccgcaatgtg ctggcggcca tggaaaaaaa
     1321 tggcgttaaa aatatcatct ttaccagttc cgttgctgtt tatggtttga acaaacacaa
     1381 ccctgacgaa aaccatccac acgatccttt caaccactac ggcaaaagta agtggcaggc
     1441 agaggaagtg ctgcgtgaat ggtataacaa agcaccaaca gaacgttcat taaccatcat
     1501 ccgtcctacc gttatcttcg gtgaacggaa ccgcggtaac gtctataact tgctgaaaca
     1561 gatcgctggc ggcaagttta tgatggtggg cgcagggact aactataagt ccatggctta
     1621 tgttggaaac attgttgagt ttatcaagta caaactgaag aatgttgccg caggttatga
     1681 ggtttataac tacgttgata agccagacct gaacatgaac cagttggttg ctgaagttga
     1741 acaaagcctg aacaaaaaga tcccttctat gcacttgcct tacccactag gaatgctggg
     1801 tggatattgt tttgatatcc tgagcaaaat tacgggcaaa aaatacgctg tcagctcagt
     1861 gcgcgtgaaa aaattctgcg caacaacaca gtttgacgca acgaaagtgc attcttcagg
     1921 ttttgtggca ccgtatacgc tgtcgcaagg tctggatcga actctgcagt atgaattcgt
     1981 tcatgccaaa aaagacgaca taacgtttgt ttctgagtaa tgcagacgag tcagagaaaa
     2041 aaggaccagg ttggactacg attattcgga aagaggtact gattaaatta tcagtatgcg
     2101 gaattccgag taatccgagt aacccagatg ctgatggtgt gtagtgttat cctaaggttt
     2161 aagcaaagtt atgcggaact aagctttggt atatagccat aacgtttggc taacctatgg
     2221 caataattta cgcactatca gccacttaga ggtaggaaaa gaatgacgaa tttaaaagca
     2281 gttataccgg tagcaggtct tgggatgcat atgttgcctg ccactaaggc gattcccaaa
     2341 gagatgctac cgatcgtcga caagccaatg attcagtaca ttgttgacga gattgtggct
     2401 gcagggatca aagaaatcct cctggtgact cacgcgtcca agaacgcggt cgaaaaccac
     2461 ttcgacacct cttatgaatt agaatctctc cttgaacagc gcgtgaagcg tcaactgttg
     2521 gcggaagtgc agtctatctg tccgcctggc gtgaccatta tgaacgtgcg tcagggcgaa
     2581 cctttaggtt taggccactc cattttatgt gcacgacccg ccattggcga caacccattt
     2641 gtcgtggtac tgccagacgt agtgatcgac gacgccagcg ctgacccgct gcgctacaac
     2701 cttgctgcca tgattgcgcg cttcaatgaa acgggccgta gccaggtgct ggcaaaacgt
     2761 atgccgggtg acctctctga atactctgtc atccagacca aagaaccgct tgatcgtgaa
     2821 ggtaaagtca gccgcattgt tgaatttatc gaaaaaccgg atcagccgca gacgctggac
     2881 tcagacatca tggctgttgg tcgctatgtg ctttctgccg atatttggcc ggaactagaa
     2941 cgtactcagc ctggtgcatg gggacgtatt cagctgactg atgcaattgc tgagctggcg
     3001 aaaaaacagt ccgttgatgc catgctgatg accggcgaca gttacgactg cggtaaaaaa
     3061 atgggttata tgcaggcgtt tgtgaagtat gggcttagga atttaaagga aggtgataag
     3121 ttcaggatga gtgtaaaaaa attgttagaa atctaactcc gaactattaa cgcgttcctt
     3181 gggcgatagg ggacttaata cgattgtaat ggttaatcgt gtttcccctt ttttagattg
     3241 attggtagtt ggaatggaaa ttttatattg agcaaaatat ggggaagata tattttagtt
     3301 agttattaag agtacaatct gttgaatatt tgtttcgcta aataattgag ttttaggttt
     3361 ttattaaaat ttataactat taatgagcat agtgcactgg tagctataga gccaggggcg
     3421 gtagcttgtt tagtgttagg tattctggtt ttaaaagcat aaaactctcc agtattttca
     3481 ttgcatacat aatattattc attaaatgtc attaaaaaaa aatgttatat atctagtgtt
     3541 gatgcaagcg gttaattata ttgctcctct tgttttagta ccatatttaa cgcgaatcct
     3601 tggagtggaa aaatatggcg ttctaggtct tgctattacg gttagccagt acttaatttt
     3661 gttgacggat tttggtttta attttactgc cagtagaaaa atagctcaat ttaaagatag
     3721 taaagtcagg gttagtcaaa tattttggac tatcatctca gcaaaatttt taatgatgat
     3781 agtatcattc ggtttaattg tcccttttgt tgtgttttca gaaaaattaa atcccttaaa
     3841 atgggaaata tttttagttt cgctatctgt agttgcgagt gtgattattc catcttggtt
     3901 gtttcagggg cttgaaaagg ttacagtttt ttccggcatc aatatttttt ctaagatttt
     3961 gattgtgcct cttgtcttta tttttgtgaa atctaaggag gatttactga ttgcctgctt
     4021 attacaaggt ggagtgcaag tattttctgg aataatatcc atattgtatg ttaagtataa
     4081 taagattatt tcatttaaag ttgtgaggcc aaaattaatc tttatatatt taaaagagtc
     4141 tctatcagta tttttaggta atttatctat ttctttatat actctcagta cgcccttagt
     4201 tttagctctt atggggacaa cgtatcaagt tgggctttat agtgccactg atagaatcag
     4261 aggggctgca attggtatct ttatagttgt tggatatgca attttcccca gagtaagcta
     4321 tttgtttaaa aaaaatccct tagaagctaa tgttttgctt aaaaaaataa tatttatctt
     4381 ctcgattctg ggatgtttag gtggaatact tgtatatagt attgctgatg agattgtttt
     4441 agttgcattt ggtaatcaat atttagattc tgccattctt ttgaaaataa tggcgcctat
     4501 gtttttactc attcctcttt caataattat ggcaaactac ttattattgc caaatggttt
     4561 taaaaaagag tatgcaaaaa attctgtgat agtatgctta ttgcatatga tatatgtttt
     4621 ccctctttgt aaatactatg gtgcagttgg cggtagttat tcgattctta tttcagagat
     4681 aataagcttt attttattaa ttttttggac gataaaaaat aatttattga agaaggtttt
     4741 ttatgcaaga taataaaagt ataaaactaa gcgttataat accatgttgg aactgtagta
     4801 aatatatcac aaaaacattg gattctatca aatattcata ttatatgtct agaaagccaa
     4861 ttttagagat tattttagtg gatgatggct ctacagactt aacaagcaaa ataatcaaag
     4921 cgtatgactt cggaactcga gcggttaatg ttaaatatca ttttcaaaat aacgccggtc
     4981 ctagtaaggc acgaaatcaa ggaattaaat tagcgcaagg taggtatgtg actttcttgg
     5041 attcggatga tatatggagt ccagattacc ttagaatcat agagtctata atggacaaat
     5101 atgattctga aattattgaa tttaatgccg ttaggtttat tgaagaaaat gccaatttaa
     5161 aaattcataa taattataca ttagtggacg aggagtatca tggtcctata aatgaacgta
     5221 ttctttctga ggtttttata aaaagcgagt ggtatgtttg ggctcgtgtt tacaaaaaaa
     5281 aacttttgga aggagagtcc ttcaatgaaa atattacaca tcatgaagat gctgaatttc
     5341 taccacggat ttatcttaca gctaaatcaa tcagcagaat tacttcccag ttaatttatt
     5401 atagattaac gcagaatagt attacaacaa aaccaaaaat tagtagcatc aaagatctca
     5461 ctttagtatg cgaattgtat tatgaaaata tcaatgggcc aaatgacaaa tattataagg
     5521 ctgcaatgat caactgtctc tggggtttaa aaagactaat ccttgataaa ggagaattta
     5581 gttttataaa aaaaagtaga atattatatt actgccatat tgctagaaat agttcacaac
     5641 tttttaatat gttaagttgg aaaaaaagat tatttattta tcttcctcga acctatatat
     5701 ttttaaattc ccttaaatat aagctgtcaa aacgtggtgc ataatggaaa tatcaatagt
     5761 aattccactg tataacaaag agaattatat caagaggacg ttgctttcaa taattgatac
     5821 ttttgggaga gtctataacg agaaagagta tgaaatagtc atcgtggatg atgggtcaaa
     5881 agataaaagt gtaagtgtag tagaaagtat aaatagtaat gttgtaaaac tctacaaaca
     5941 agaaaatggc ggtccttcaa aagcaagaaa taatggtgta aatttcagtg atggaaaata
     6001 tgtcattttt tgtgatgcag atgatattat tttaccaaat tatggtgatt atataaaata
     6061 ttccattgat aattatcctg accatgatgt ttttgttgca tttagccgtg ttttaagaaa
     6121 cagtaaagaa aattatagca tacctctttt tgaccccgtg gatgtttcta ttgttgatga
     6181 ttttttctat gagtgggata agagaaagtt ttttagtgca tcgtcaattt gtataaaaaa
     6241 acatttcttt atacaaaatg acttgtattt tgatgaaacg atttcatcag gtgaagattt
     6301 attgatgttt tataaagctg cgattaaaac taaatatgtt gcctatagtc aaccggcagt
     6361 attatatgat aaaacagtag attcgcagtt aagttctaat cctgatttga aaattggtgc
     6421 acatacttat tttttaataa aagtctacaa aacagagaat ttatcagtca aagataaaac
     6481 tgcaataaat cgaattatcg ataaacaaat atgctttgtt gcagtggata atctcttacg
     6541 taaaaagtat gcaaattcgt taaatatagt attgttccgc aaacgaatgc ttttacaacc
     6601 taaattagct ataaaattaa ttatagccag tatttcgtat aaacttttta ctaaaatatc
     6661 tagtctacta agatgatcat agagaataat aaagtgagtt cacatagcta tttatgggtt
     6721 cttttccaat cctttatatt ttattacaaa ttcatgctcc aaagttggga cggtgatgaa
     6781 cagattgtaa atgtaatatc tatcatctta accttacttc ttttatgtgg tacgattagt
     6841 gcttttataa tctcaaatat taaagagaaa tgtatatttt taataatttt catttttgtt
     6901 gccttgaaca taattatagc agataacaaa tctgtctttt ggatggtgac ggcgttcact
     6961 ttcttgatac tcttttcaac gttaaccatt aaaaatagaa ttaggatatt ggttttctct
     7021 tttataattg catggtgttt ctttttacca ctacaaatat ttttctccaa ttcctataca
     7081 tatattgatg atcgatattt aagatataca tttggatttt taaatcctaa tggattaggg
     7141 atgtttctat tactgttgca gacactttta tattattgga tatggacttc aataaaagca
     7201 acaataatcg tgaagcaaat gattacaata attttgggtg gatctatcat atgcataata
     7261 tttttaagtg aatcaagaac atatatatta ctatcctttt tattactgat tttaactgta
     7321 atatacggat ttaagaaatt taaattctca tcacgacttc tatttatata tttactgttt
     7381 gttatgcttt tacaatggct ttctgtaaaa ggttttgaga attacctcat atttcaagat
     7441 atgaacgcat atatgagtgg gcgagtctgg ttttcctata atttactaag ccaaatggga
     7501 gaaccgaaat tctttattgg tagtgatatt tcattgtatc aaccgataga cttcttcttt
     7561 atatcactat tatataataa tgggatactg gcttcgttaa tattgttata ctgtaactat
     7621 atctttctaa aaaagttaga caactctacg aaatatgaaa gtatattagc ttttatattt
     7681 attacagtaa gtttcacaga agctgtatat aatattcctc tgttgaattt cttctttttg
     7741 ctgttatata aaaaggaatt gaggttttca tgaaagttga tgcagtaatc ttatgggtag
     7801 atggtaatga tccaaaatgg caggaagaat acaataaata ttgtaagccg gcctctcgaa
     7861 ttgagaatgg tgtacaaaga tatagagatt gggatacact acgatatgta attcgtggaa
     7921 tagaatataa ccttccttgg attgacaaaa tacattttgt gacatgtggt caaaagccaa
     7981 gttggatggt tggatatcat ccaaaactca attttgtaca tcataacgat atttttgaga
     8041 atgatacatt tttacccaca tttaattcaa gtgcaataga gttaaatctg tcaaggataa
     8101 agggattaag tgagaggttt atatatttta atgatgatat gcttgtgctt aaaaacactc
     8161 ctcttcagag gttttttgta aatgatttac cggtggattt tttaattgaa gctttcccac
     8221 ggcgtgggtt attgtatgaa aaaatacgtt ccaactcaac ttgggtttca atgataaata
     8281 attgcacttc gctcataaat agagtgtatc acaagaataa atatatacag gataatcgta
     8341 atttatatta taacatgaac tatggacgac atgtcattgc taatgtactg gcgtcccctt
     8401 ttaagcagtt tttggctttt aaacactatc atcatcctca agcatatctt aaaaagacat
     8461 tacaatccgt tgagcgtgag ttcccagtgg agtttaactt aacatgcaaa tctagattcc
     8521 gagaacatga taatatctca caggcgttgt ttagatatta ccaattggta actggtagtt
     8581 tttatccgtg ctattataat gatcatgcct gtgttaatgt agtaaataaa aaatctgcaa
     8641 gccagtgtat tgaagctctt catcaaaaac gatttgtgtg tataaatgat gagattaatg
     8701 acgacttaga tgatagttca attttaataa atgatataat taaagagttg gatttgattc
     8761 ttccaaataa atcaagtttt gagatctaaa ttaatatgaa ttcgtttgat gagtttaatg
     8821 tgctactatc tctttataaa aatgagagcc ccgataatct ggatgcgtgt tttcaaagca
     8881 taagcactca atctttgaaa agatttaaaa taatacttgt catagatggt cctatttctt
     8941 ctgagttaaa tgaggttgtc ggaaagtgga agtcactatt acctattaaa attatcaatc
     9001 tcgaacgtaa tgttggctta ggtaatgctt taaatatagg tttgaaatat tgcagttgtg
     9061 attatgtgtt tcgtatggat acagatgata tttgtcatcc ggataggttt tccatacaat
     9121 ttagttatct gaggaaacac cccgatattg atttgcttgg tggacagatt gtagaattcc
     9181 atgaatgcat agaggagcct aacggtatgc ggttagtacc ttctaaatat gaggaaattc
     9241 ttcagtattg taaacttaaa aacccattta atcatatgac agttgtgttt aaaagagaga
     9301 gtgtacttaa ggtcggtgga tataaacatc atttatatat ggaggattat aatctttggc
     9361 ttcgtatgat atcaattggt tgcaaagtgg aaaatttaga tgatgtgatt gtttttgccc
     9421 gaacagacgt gaattcatta atgagacgta gagggtggca atatgttaaa agtgaatgga
     9481 aattagcact gttaaaaata aagttaagaa taaataatcc tattgtttct ttatctgttt
     9541 ttatcctccg atctatacct cgattattac ctattatgtt gattcgtaga atttatgccc
     9601 acaaccgcaa gtgaattttt ttaagtcata ttgagtaatt gagtaatagt tttcctatgt
     9661 aatcaaaatt aactatcagt tttattatta gttgggtaat atctctctat atctcaacca
     9721 gtgcagtcat tgcatggtga acacccctga caggagcaaa caatgtcaaa gcaacagatt
     9781 ggcgtcgtcg gtatggcagt gatggggcgc aaccttgcgc tcaacatcga aagccgtggt
     9841 tataccgtct ctattttcaa ccgttcccgt gaaaagacgg aagaagtgat tgccgaaaat
     9901 ccaggcaaaa aactggttcc ttactatacg gtgaaagagt ttgttgaatc tctggaaacg
     9961 cctcgtcgca tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc tattgattcc
    10021 ctcaagccat acctcgataa aggtgacatc atcattgatg gtggtaatac tttcttccag
    10081 gacaccattc gtcgtaaccg tgaactttct gccgaaggct ttaacttcat tggtaccggt
    10141 gtttccggtg gtgaagaagg cgcgctgaaa gggccttcca ttatgcctgg tggccagaaa
    10201 gaagcctatg aactggttgc accgatcctc accaaaatcg ccgcagtggc tgaagacggt
    10261 gagccatgcg ttacctatat tggtgccgat ggagcaggtc actatgtgaa gatggttcac
    10321 aacggtattg aatacggcga tatgcagctg attgctgaag cctattctct gcttaaaggt
    10381 ggcctgaatc tttccaacga agaactggcg cagacgttta ccgagtggaa taacggtgaa
    10441 ctgagcagct acctgatcga catcaccaaa gatatcttca ccaaaaaaga tgaagacggt
    10501 aactacctgg ttgatgtgat tctggatgaa gcagcaaaca aaggtacggg caaatggacc
    10561 agccagagtg cgctggatct cggcgaaccg ttgtcgctga ttaccgagtc tgtgtttgct
    10621 cgttatatct cttctctgaa agatcagcgt gttgccgcat ctaaagttct ctctggcccg
    10681 caagcacagc cagcaggcga caaggctgag ttcatcgaaa aagttcgtcg tgcgctgtat
    10741 ctgggcaaaa tcgtttctta cgcccagggc ttctctcagc tgcgtgctgc gtctgaagag
    10801 tacaactggg atctgaacta cggcgaaatc gcgaagattt tccgtgctgg ttgcatcatc
    10861 cgtgcgcagt tcctgcagaa aatcaccgat gcatacgccg aaaatccgca gatcgctaat
    10921 ctgctgctgg ctccgtactt caagcaaatt gccgatgact accagcaggc gctgcgcgat
    10981 gtcgtcgctt atgcggtaca gaacggtatc ccggttccga ccttcgccgc tgcggttgcc
    11041 tattatgaca gctaccgcgc cgcagttctg cctgcgaacc tgatccaggc acagcgtgac
    11101 tatttcggtg cgcatactta taagcgcatt gataaagaag gtgtgttcca taccgaatgg
    11161 ttggattaat ctgatctaac caacaataaa tttaggcccg gcgtatattg cgccgggctt
    11221 tttgtgcaca gaaaaccccc agctaggctg ggggttccgg aaagctatca gctttgagcc
    11281 agttattaaa accccttttg atttgttaaa acaccttgcg gtctggcaac tgcaagtgtc
    11341 aaacaagaaa tcaaaagggg gtcccaatgg ggaacgaaaa gagcttagcg cacacccgat
    11401 ggaactgtaa atatcacata gtttttgcgc caaaatactg aagacaagtg ttctacagag
    11461 agaaacgtag agcaataggc agtattttga gaaagctgtg tgagtggaaa agcgtacgga
    11521 ttcttgaaac tgaatgctgt gcagatcata tccatatgct tgtggagagc ccgcccaaaa
    11581 tgagcgtatc cggctttatg ggatatctga aagggaaaag cagtctgatg ctttacgagc
    11641 agtttgatga tttgaaattc aaatacagga acagggagtt ctggtgcaga gggtactacg
    11701 tcgatacggt gggtaagaac acggcgaaga tacaggatta cataaagcac cagcttgaag
    11761 aggataaaat gggagagcag ttatcgattc cctatccggg cagcccgttt acgggccgta
    11821 agtaacgaag ttggatacaa atgtcagatc gtgtgcgcct gttagggcgc ggctggtaag
    11881 agagccttat aggtgcattt gaaaaacctc cggctatgcc ggaggatatt tatttttgcc
    11941 aattatctta tagactaaat ttcactgctt aatattaact taataaatat cggctattct
    12001 tataaagaaa atctgaattg tttttcgttg cgttgaccat ctgacaacgt agcgttaaaa
    12061 cttttagctc ttatcaggat gctaaaaaca tcatgattca cagttaagtt aattctgaga
    12121 gcatgaaatg aaaatcacca tttccggtac tggctatgta ggcttgtcaa acgggcttct
    12181 aatcgcacaa aatcatgagg ttgtggcatt agatatttta ccgtcacgcg ttgctatgct
    12241 gaatgatcgg atatctccta ttgttgataa ggaaattcag cagtttttgc aatcagataa
    12301 aatacacttt aatgccacat tagataaaaa tgaagcctac cgggatgctg attatgtcat
    12361 catcgccact ccaaccgact atgatcctaa aactaattat ttcaatacat ccagtgtaga
    12421 atcagtaatt aaagacgtag ttgagataaa tccttatgcg gttatggtca tcaaatcaac
    12481 ggttcccgtt ggttttaccg cagcgatgca taagaaatat cgtactgaaa atattatttt
    12541 ctctccggaa ttcctccgcg aaggtaaagc cctttacgat aatctccatc cgtcacgtat
    12601 tgtcatcggt gagcgttcag aacgcgcaga acgttttgct gcgttattac aggaaggcgc
    12661 gattaagcaa aatatcccga ccctgtttac cgattcaacc gaagcagaag cgattaaact
    12721 tttcgctaat acctatctgg cgatgcgcgt agcatacttt aatgaactgg atagctatgc
    12781 agaaagttta ggtctgaata ctcgccagat tatcgaaggc gtttgtctcg atccgcgtat
    12841 tggcaaccat tacaacaatc cgtcgtttgg ttatggtggt tattgtctgc cgaaagatac
    12901 caagcagtta ttggcgaact accagtctgt gccgaataac ctgatttcgg caattgtcga
    12961 tgctaaccgc acgcgtaaag actttattgc cgatgccatt ttgtcacgca aaccgcaagt
    13021 ggtgggtatt tatcgtctga ttatgaagag cgggtcagat aacttccgtg cgtcttccat
    13081 tcaggggatt atgaaacgta tcaaggcgaa aggcgttgaa gtgatcattt acgaaccggt
    13141 gatgaaagaa gactcgttct tcaactctcg gctggaacgt gatctcgcca ctttcaaaca
    13201 acaagccgaa gtcattattt ccaaccgtat ggcagaagag cttaaggatg tggcagacaa
    13261 agtctacacc cgcgatctct ttggcagtga ctaacatcct gttatcaggg cgattttcgc
    13321 cctgattctc ttatgttccc tttgtaataa ttcattattt ttatcattta tcctatagca
    13381 ttcatggtga ttatcgctaa actatggcga cttaaaatta tttcgtccgt tagggtaatg
    13441 atgagagtag aaaataataa tgtttctggg caaaaccatg acccggaaca gattgatttg
    13501 attgatttac tggtgcagtt gtggcgcggc aagatgacaa ttatcatctc tgtcattgtc
    13561 gctattgccc tggctatagg gtatttggcc gttgccaaag aaaagtggac ttccacagct
    13621 atcgttactc aacctgatgt ggggcaaatc gctggttata ccaacgcgat gaatgtgatt
    13681 tacggtccgg ctgtaccgaa agtctccgac attcagactt cacttatcgg acgttacagc
    13741 actgcattct cagcattagc ggaaacgctg gataatcagg aagaacctga aaaactgacc
    13801 attgagccta ccgttaaaaa tcagtcctta cctttagcgg tttcttatgt tggtgaaaca
    13861 gctgaaggtg cgcagaaaca gctggcgcag tatatccagc aagttgacga ccaggtaaac
    13921 gaagaactgg aaaaagacct gaaagacaac atcgcactgc gtatgaaaaa cttgcaggat
    13981 tcgcttaaga cccaggaagt ggttgcgcag gagcagaaag atctgcgtat ccgtcagatt
    14041 caggaagcgt tgcagtatgc gaatcaggcg caggtgacaa aaccacaggt tcagcagact
    14101 gaagatgtga cgcaagatac gttgttcctt ttggggagcg aagcgctgga gtcgatgatt
    14161 aagcatgagg cgacccgtcc gttggtgttc tcatcaaact actatcagac acgtcaaaac
    14221 ctgctggata tcgaaagctt aaaggttgat gatcttgata ttcatgctta ccgctatgta
    14281 atgaaaccga cgttacctat tcgtcgcgat agcccgaaaa acgcaattac cttgattttg
    14341 gcggtgctgc tgggcggcat ggttggcgcg gggattgtgc tggggcgtaa cgctctgcgt
    14401 aattacaacg cgaaataatc tttgcagctt taaagaaaac gggcagggtg gtgacacctt
    14461 gtccgttttt tttgccggat gcgacaacaa catcgcatcc gcttaccccg ccactcactg
    14521 atgccgttta cgcaggttct caattaccgt cgttaaatcc agatcctgat cctgcaacaa
    14581 caccagcagg tgatacatca agtcagatgc ctcgttggtc agctcaaagc ggtcatgtac
    14641 cgttgcagcc agtgcggttt ccacgccttc ttcgcccact ttctgcgcaa tgcgtttggt
    14701 gccgctggca tacagtttgg cggtgtagga ggtttccggg tcggcagatt tgcgttcggc
    14761 gagcagttgt tccagttgat acaggaacag ccactggtga gcggtgtcgc cgaagcagct
    14821 gctggtgcct ttgtggcagg t
//
