LOCUS       X78853                  3536 bp    DNA     linear   BCT 18-APR-2005
DEFINITION  S.xylosus malR gene and malA gene.
ACCESSION   X78853
VERSION     X78853.1
KEYWORDS    alpha-D-1,4-glucosidase; alpha-glucosidase; malA gene; MALR gene;
            regulator protein.
SOURCE      Staphylococcus xylosus
  ORGANISM  Staphylococcus xylosus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Egeter,O. and Bruckner,R.
  TITLE     Characterization of a genetic locus essential for
            maltose-maltotriose utilization in Staphylococcus xylosus
  JOURNAL   J. Bacteriol. 177 (9), 2408-2415 (1995)
   PUBMED   7730272
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Egeter,O.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-1994) O. Egeter, Abteilg. f. Mikrobielle Genetik,
            Uiversitaet Tuebingen, Auf der Morgenstelle 28, 72076 Tuebingen, FRG
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Staphylococcus xylosus"
                     /mol_type="genomic DNA"
                     /strain="DSM 20267"
                     /isolate="cured strain C2a"
                     /db_xref="taxon:1288"
                     /clone="pRA5"
                     /clone_lib="genomic in E.coli"
     regulatory      252..289
                     /regulatory_class="terminator"
                     /note="rho independent"
     regulatory      358..363
                     /regulatory_class="minus_35_signal"
     regulatory      381..386
                     /regulatory_class="minus_10_signal"
     regulatory      427..433
                     /regulatory_class="ribosome_binding_site"
     gene            439..1452
                     /gene="malR"
     CDS             439..1452
                     /gene="malR"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|LacI,STP|Peripla_BP_1,STP|Peripla_BP_3"
                     /protein_id="CAA55408.1"
                     /db_xref="GOA:Q56201"
                     /db_xref="InterPro:IPR000843"
                     /db_xref="InterPro:IPR001761"
                     /db_xref="InterPro:IPR010982"
                     /db_xref="UniProtKB/Swiss-Prot:Q56201"
                     /translation="MVTIKDIAQAANVSTSTVSRVISGNPRISMQTREKVKATMKSFNY
                     QPNRAARTLATKQSNTIGIIQKSASIEDSQNPFVLDVLSGIFSKCKNHGYATISTTKGQ
                     SIEIELEVQEMIHYHSVDGFIVLYSKKSDPIIDILKSHAMPYVIIGKPLTDDDIIHIDN
                     DNVSASQSLTRYLIDKGHNKFLFVAETGNYEVVKDRIAGHLNAIEQTDSVTDIVYFAKN
                     RHYIRSFFQDLIEHRTLPTVVITSDTLLNHLILSVFYELKLHIPTDIQTATFNDSYLNA
                     FASPPQTTVDIYPKLLGEGAAESAINIIQGHNILNFYKLIPTTIIERESTTTIQEV"
     regulatory      1442..1447
                     /regulatory_class="ribosome_binding_site"
                     /gene="malR"
     gene            1454..3103
                     /gene="malA"
     CDS             1454..3103
                     /gene="malA"
                     /EC_number="3.2.1.20"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13_31|GH13"
                     /protein_id="CAA55409.1"
                     /db_xref="GOA:Q60015"
                     /db_xref="HSSP:1UOK"
                     /db_xref="InterPro:IPR006046"
                     /db_xref="InterPro:IPR006047"
                     /db_xref="InterPro:IPR006589"
                     /db_xref="InterPro:IPR013780"
                     /db_xref="InterPro:IPR013781"
                     /db_xref="InterPro:IPR017853"
                     /db_xref="UniProtKB/TrEMBL:Q60015"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNNDGIGDLPGVIEKLDYLNELGI
                     DVIWLSPMYKSPNDDNGYDISDYTDIMDEFGTMDDFNLLLESVHQRGMKLILDLVVNHT
                     SDEHPWFIESKSSKDNPKRDWYIWQEPKPDGSEPNNWESIFNGSTWEYDDTTGEYYFHL
                     FSKKQPDLNWGNKEVRHAIFEMMNWWFEKGIDGFRVDAITHIKKSFEAGDLPVPEGKTY
                     APAFDVDMNQPGIQSWLQEMKEKSLSQYDIMTVGEANGVNPENAVEWVGENEGKFNMIF
                     QFEHLGLWNTGDSKFDVKAYKDVLNRWQKQLENIGWNALFIENHDQPRRVSTWGDDQNY
                     WYESATSHAIVYFLQQGTPFIYQGQEIGMTNYPFESVETFNDVAVVNEYNIVKSQNGDV
                     SALLEKHKMENRDNSRTPMQWNNQTNSGFSEHSPWFPVNPNYKTINVADQQQDPNSILN
                     FYKSLITLKKSDDIYTYGTFDLVDKNNEQVFAYTRTLNNKKVLIVANLTNQTATFKYDN
                     SINQDNILLHNYKNEAIDINAMKPFEAFVLST"
     regulatory      3265..3299
                     /regulatory_class="terminator"
                     /note="rho independent"
ORIGIN
        1 gaattatcaa gatgcattac gtgatgtagt tgcaactggt gttcaaaatg tgtaccaaca
       61 cctggtttct ctgcaagtat taactattat gatagttatc gctcagaaaa cttaccagct
      121 aacttgatcc aagctcaacg tgattatttc ggcgcacata cttatgagcg taaagatcgc
      181 gatggcgtat tccatacaca atggattgaa gattaagatt aactgttgat ttaattttat
      241 aaaaaatgaa ctacccgtaa ccaaagacat gtgtctttag ttgcgggtat tttttgtatc
      301 ttataacttt ttctaaattt ttaaaaatga atttgtgcaa acgcttgcac aaattctttg
      361 acatgctgaa tagctttaat tattataaga gttagttaca taaggtttcg tttggaaact
      421 aaacaaagga aggcgtttat ggtcacaatt aaagacattg cccaagcagc aaatgtttca
      481 acttcaactg tttcaagagt tattagtggc aacccaagaa ttagtatgca aacacgagaa
      541 aaagtaaagg caactatgaa atctttcaat tatcaaccaa accgagcagc gagaacgtta
      601 gcaacaaaac aatcaaatac aattggtatt atacaaaagt ctgcttctat tgaagatagt
      661 caaaacccat ttgttttaga tgtactttct ggtattttta gtaaatgtaa aaatcatggg
      721 tatgccacaa tttcaactac aaaaggacag agtattgaga tagaactaga agtccaagaa
      781 atgattcatt atcattctgt tgatggcttt atagtacttt attctaaaaa gagtgatcct
      841 ataattgata tattaaaatc acatgccatg ccatatgtga ttattggtaa acctttaaca
      901 gatgatgaca ttatccatat agataatgac aatgtaagtg catcgcaatc attaacacgg
      961 tatttaattg ataaaggaca taataaattt ttatttgtag cagaaacggg taactatgaa
     1021 gttgtaaaag ataggatagc aggacattta aacgctatag aacaaaccga ttcagtaacc
     1081 gatattgtgt attttgctaa aaatcgccac tatattagat cattttttca agatcttatt
     1141 gaacatcgta cattaccgac tgtcgtaatt acttccgaca cactactgaa tcacctaata
     1201 ttaagtgttt tttatgaatt aaagttgcat atacctacag atattcaaac tgcaacattt
     1261 aatgattctt atttaaatgc atttgcatcc ccgccacaaa caactgtgga tatttatccg
     1321 aaattactcg gagaaggagc agctgaatca gctataaata ttatacaagg tcacaatatc
     1381 ttaaattttt ataaattaat accaacaaca attattgaac gtgaatcaac cacaacaatt
     1441 caggaggtat agtatgaaca aacaatggtg gaaagaagca gtagcgtatc aagtttatcc
     1501 aagaagtttt aatgattcta ataatgatgg tatcggtgac ttacctggag ttattgaaaa
     1561 gttagattac ttaaatgaac taggcattga tgtgatatgg ttaagcccta tgtataaatc
     1621 tcctaatgat gataacggct atgacattag tgattatact gatattatgg atgaatttgg
     1681 aacaatggac gattttaatt tattgttaga aagtgttcat caacgtggca tgaaattaat
     1741 tcttgatcta gttgttaatc atacttctga tgagcatcca tggtttatag aatctaaatc
     1801 cagcaaagat aaccctaaac gtgattggta tatatggcaa gaaccaaaac ctgatggctc
     1861 agaacctaat aattgggaaa gtatatttaa tggttctaca tgggaatatg atgacactac
     1921 aggtgaatat tacttccact tatttagcaa aaagcaacca gacttaaatt ggggcaataa
     1981 agaggtgcgt catgctattt tcgaaatgat gaattggtgg tttgaaaaag gaatcgatgg
     2041 ttttagagta gatgcaatca ctcatattaa gaaatcgttt gaagcaggag atttacctgt
     2101 accagaagga aaaacttatg ctcctgcatt tgatgtagat atgaatcaac ctggaattca
     2161 aagttggtta caagaaatga aagaaaaatc attaagccag tacgatatta tgacagtcgg
     2221 tgaagcaaat ggtgtaaatc cagaaaatgc agtggaatgg gtaggggaaa atgaaggcaa
     2281 gtttaatatg atattccaat ttgagcattt aggtttatgg aatactggtg attctaaatt
     2341 tgacgttaaa gcctataaag atgttcttaa tagatggcag aaacaattag agaatatagg
     2401 atggaacgca ctgtttatag agaaccatga ccaacctcgt cgcgtctcaa catggggaga
     2461 cgaccaaaat tattggtatg aatcagctac aagtcatgct attgtctatt tcttacaaca
     2521 aggtacacct tttatttacc aaggacaaga aatcggaatg acaaactatc cttttgaaag
     2581 tgttgaaaca tttaatgatg ttgcagtagt taacgaatat aatattgtta aatcacaaaa
     2641 tggagatgtt tcagcattat tggaaaaaca taaaatggaa aacagagata attcaaggac
     2701 accaatgcaa tggaacaatc aaacaaatag tggcttttca gaacatagtc cttggttccc
     2761 ggtaaatcca aattataaaa caattaatgt tgctgaccag cagcaagatc caaattcaat
     2821 attaaacttt tataaatcat taattacctt aaaaaaatca gatgatatat acacatacgg
     2881 tacatttgac ttagtagata agaataatga gcaagtattt gcttatacga gaacactaaa
     2941 caacaaaaaa gtactaattg tagctaattt aacaaatcag actgctacgt tcaaatatga
     3001 taattcaata aatcaggata atattttact tcataattat aaaaacgaag caatagatat
     3061 taatgctatg aagccttttg aagcgtttgt tttatcaacg taataattaa cgtttgagaa
     3121 aaatcataga ctagctattg aaagtttttc tataaaaaaa gcataggtaa acgatatcta
     3181 aatttctatt aaagatatcg tcagcctata ctcacaatga tattttatta tattaaatat
     3241 agctttagaa tgtgttttta atcttactgc cagcaaagca tctatttttg ttggcagtat
     3301 ttaattttgt actttaaaat tttgtatttt tcacataaac ataccatcaa acgttagtaa
     3361 tgatactact taaaaatcgt cagttaaata ttgttcctgg tcgacaggca tccataattg
     3421 tactttagtg aaggggtcgt caaatgaaat atcgaaaggg taaatttcta cataaagact
     3481 attgtgttcg tacggtaaag tcatctgtaa gctagtttca atataatacc aagctt
//
