LOCUS       X77617                  8025 bp    DNA     linear   BCT 23-OCT-2008
DEFINITION  E.coli kfiD, kfiC, kfiB and kfiA genes.
ACCESSION   X77617
VERSION     X77617.1
KEYWORDS    glycosyltransferase; kfiA gene; kfiB gene; kfiC gene; kfiD gene;
            UDP-glucose dehydrogenase.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Petit,C., Rigg,G.P., Pazzani,C., Smith,A., Sieberth,V., Stevens,M.,
            Boulnois,G., Jann,K. and Roberts,I.S.
  TITLE     Region 2 of the Escherichia coli K5 capsule gene cluster encoding
            proteins for the biosynthesis of the K5 polysaccharide
  JOURNAL   Mol. Microbiol. 17 (4), 611-620 (1995)
   PUBMED   8801416
REFERENCE   2  (bases 1 to 8025)
  AUTHORS   Roberts,I.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-1994) I.S. Roberts, University of Leicester, Dept
            of Microbiology, PO Box 138, Medical Sciences Building, University
            Road, Leicester LE1 9HN, UK
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="K5"
                     /isolate="2980"
                     /db_xref="taxon:562"
     gene            complement(227..1405)
                     /gene="KfiD"
     CDS             complement(227..1405)
                     /gene="KfiD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase"
                     /protein_id="CAA54708.1"
                     /db_xref="GOA:Q47329"
                     /db_xref="InterPro:IPR001732"
                     /db_xref="InterPro:IPR008927"
                     /db_xref="InterPro:IPR013328"
                     /db_xref="InterPro:IPR014026"
                     /db_xref="InterPro:IPR014027"
                     /db_xref="InterPro:IPR014028"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR017476"
                     /db_xref="UniProtKB/Swiss-Prot:Q47329"
                     /translation="MFGTLKITVSGAGYVGLSNGILMAQNHEVVAFDTHQKKVDLLNDK
                     LSPIEDKEIENYLSTKILNFRATTNKYEAYKNANYVIIATPTNYDPGSNYFDTSSVEAV
                     IRDVTEINPNAIMVVKSTVPVGFTKTIKEHLGINNIIFSPEFLREGRALYDNLHPSRII
                     IGECSERAERLAVLFQEGAIKQNIPVLFTDSTEAEAIKLFSNTYLAMRVAFFNELDSYA
                     ESFGLNTRQIIDGVCLDPRIGNYYNNPSFGYGGYCLPKDTKQLLANYQSVPNKLISAIV
                     DANRTRKDFITNVILKHRPQVVGVYRLIMKSGSDNFRDSSILGIIKRIKKKGVKVIIYE
                     PLISGDTFFNSPLERELAIFKGKADIIITNRMSEELNDVVDKVYSRDLFKCD"
     gene            complement(1447..3009)
                     /gene="KfiC"
     CDS             complement(1447..3009)
                     /gene="KfiC"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="CAA54709.1"
                     /db_xref="GOA:Q47330"
                     /db_xref="HSSP:1QGQ"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="UniProtKB/TrEMBL:Q47330"
                     /translation="MNAEYINLVERKKKLGTNIGALDFLLSIHKEKVDLQHKNSPLKGN
                     DNLIHKRINEYDNVLELSKNVSAQNSGNEFSYLLGYADSLRKVGMLDTYIKIVCYLTIQ
                     SRYFKNGERVKLFEHISNALRYSRSDFLINLIFERYIEYINHLKLSPKQKDFYFCTKFS
                     KFHDYTKNGYKYLAFDNQADAGYGLTLLLNANDDMQDSYNLLPEQELFICNAVIDNMNI
                     YRSQFNKCLRKYDLSEITDIYPNKIILQGIKFDKKKNVYGKDLVSIIMSVFNSEDTIAY
                     SLHSLLNQTYENIEILVCDDCSSDKSLEIIKSIAYSSSRVKVYSSRKNQGPYNIRNELI
                     KKAHGNFITFQDADDLSHPERIQRQVEVLRNNKAVICMANWIRVASNGKIQFFYDDKAT
                     RMSVVSSMIKKDIFATVGGYRQSLIGADTEFYETVIMRYGRESIVRLLQPLILGLWGDS
                     GLTRNKGTEALPDGYISQSRREYSDIAARQRVLGKSIVSDKDVRGLLSRYGLFKDVSGI
                     IEQ"
     gene            complement(3342..3614)
                     /gene="hypothetical ORF"
     CDS             complement(3342..3614)
                     /gene="hypothetical ORF"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA54710.1"
                     /db_xref="UniProtKB/TrEMBL:Q47331"
                     /translation="MNSVFPWGGSHIGISRILSCAAVNILPMVEMLGQTRRCCSLHGFK
                     QTKICSSRLGSSILSRRISYRITRCHCFVRCTSGALLREIGASGP"
     gene            3705..4007
                     /gene="hypothetical ORF"
     CDS             3705..4007
                     /gene="hypothetical ORF"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA54707.1"
                     /db_xref="UniProtKB/TrEMBL:Q47328"
                     /translation="MGELRCYVITMIDEHSNYALAQAVPSLNCDIIKLFFCRADRLFPI
                     DINQGIADNGKEFPGNFDKAPQESASKPYGPTPLRLERTSERFKRTPKEQFIEFK"
     gene            4301..5992
                     /gene="KfiB"
     mRNA            4301..5992
                     /gene="KfiB"
     repeat_region   4711..4795
                     /gene="KfiB"
     repeat_region   4796..4879
                     /gene="KfiB"
     gene            complement(6424..7140)
                     /gene="KfiA"
     CDS             complement(6424..7140)
                     /gene="KfiA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA54711.1"
                     /db_xref="UniProtKB/TrEMBL:Q47332"
                     /translation="MIVANMSSYPPRKKELVHSIQSLHAQVDKINLCLNEFEEIPEELD
                     GFSKLNPVIPDKDYKDVGKFIFPCAKNDMIVLTDDDIIYPPDYVEKMLNFYNSFAIFNC
                     IVGIHGCIYIDAFDGDQSKRKVFSFTQGLLRPRVVNQLGTGTVFLKADQLPSLKYMDGS
                     QRFVDVRFSRYMLENEIGMICVPREKNWLREVSSGSMEGLWNTFTKKWPLDIIKETQAI
                     AGYSKLNLELVYNVEG"
ORIGIN
        1 ctttggatat agcacataat atacatttcc aacgcacaag ccaagtattc taattaacat
       61 tagagttatg aaaaattcga tataaacaca acagcagggc aagaaattta accaacatct
      121 tattttacga tcgaggatta ataaagaaca taccaacgag gcatattttt aacggattaa
      181 ataatgcatt tccttctctt aatagttaat agtatataac aatacattag tcacatttaa
      241 acaaatcgcg actatagact ttgtcgacca catcgttcaa ctcctctgac attcggttag
      301 tgataataat atcagctttc cctttaaaga tcgccagctc ccgttccaaa ggtgagttaa
      361 agaatgtatc tccagaaata agcggctcat aaataattac tttcacgcct tttttcttga
      421 tacgctttat aataccaaga atagaagaat ctctaaaatt atctgaacca cttttcataa
      481 tcaaacgata aacccccaca acttgtggtc tatgtttcaa aataacatta gtgataaagt
      541 ccttacgtgt acggttagca tcaacaattg cagatataag tttattcgga acagactgat
      601 agttggctaa taattgcttg gtatcttttg gcaaacagta gccaccataa ccaaaagaag
      661 gattattgta gtaattacca atgcgcggat ccaaacaaac accgtcaata atctgacgcg
      721 tattcagacc aaaactttct gcgtaactat ccaattcatt aaaaaatgca actcgcatag
      781 ccaaataagt atttgaaaat aacttaatcg cttccgcttc cgtagaatct gtaaataaaa
      841 cgggtatatt ttgtttaatc gctccttcct gaaataacac tgccaaacgt tctgcccgtt
      901 cagaacattc accgataata atgcgagatg gatggagatt atcgtatagg gctcttcctt
      961 ctcgtaaaaa ttctggagag aagataatat tattaatacc taaatgttct ttaattgttt
     1021 ttgtgaaacc tactgggacc gtagatttaa ccaccataat tgcgtttggg ttgatttccg
     1081 ttacgtcacg aatgacagct tcaacgcttg atgtatcaaa gtaatttgaa cctgggtcat
     1141 aattcgttgg tgtagcaata ataacgtaat tggcattttt ataggcttca tatttgttag
     1201 tagttgcgcg aaaattaagt attttagttg aaagataatt ttcaatttcc ttatcctcta
     1261 taggagagag tttatcatta agtaagtcaa cttttttttg atgggtatca aatgcaacca
     1321 cttcatgatt ttgagccatt agaattccat ttgaaagccc aacgtaacca gcgcctgaaa
     1381 cagttatttt tagtgttccg aacattttat atagccccaa aaatttaata tatatagaat
     1441 aacaaactat tgttcaatta ttcctgatac atctttaaac aaaccatagc gagataataa
     1501 accacgtaca tctttatcac ttacgatact tttccctaac actcgttgtc ttgccgcgat
     1561 atcactatat tctcttcgag attgtgatat atatccatca ggtagagctt ctgttccttt
     1621 attcctggta agtccggagt ctccccataa ccccaatatc aatggctgca gtaatcttac
     1681 aatactttct cgcccataac gcattattac tgtttcataa aactccgtat ctgcaccaat
     1741 taaagattgt ctatagccac caactgtcgc aaaaatatct ttttttatca ttgacgatac
     1801 aacagacatt cttgtggctt tatcatcata gaagaattga atttttccat ttgacgcaac
     1861 acggatccag ttagccatac agattacagc cttattattg cgaagaacct caacttgtct
     1921 ttgtattctc tccggatgag aaagatcatc tgcatcttga aaggtgatga aattaccgtg
     1981 tgcttttttt attagctcat ttcttatatt ataagggcct tggttttttc gtgagctata
     2041 tactttcact cttgaactag aataagctat gctcttaatt atttcaaggc ttttgtccga
     2101 tgaacaatca tcgcacacga gaatttcaat attttcatat gtttgattca acaatgaatg
     2161 taatgagtat gcaatagtat cttctgaatt gaatactgac attattatac taacaagatc
     2221 ttttccataa acattttttt tcttatcgaa cttaattcct tgcaatataa ttttatttgg
     2281 gtatatatca gttatttctg ataaatcgta ttttcgtaga catttgttaa attgactcct
     2341 ataaatattc atattatcta ttacagcatt acaaataaaa agttcttgct cagggagtag
     2401 attataacta tcttgcatat catcgtttgc atttaataat aaagtcaggc cataccctgc
     2461 atcggcttga ttatcaaatg ctaaatattt atatccattt ttagtataat catgaaattt
     2521 tgaaaacttc gtacaaaaat aaaaatcttt ttgtttgggc gacaatttta gatggtttat
     2581 atattcgata tatcgttcaa aaataagatt aatgagaaaa tcactccttg aataccgtag
     2641 agcgttactt atatgttcaa aaagcttaac tcgttcgcca tttttaaaat aacgagattg
     2701 aattgttaga taacaaacaa ttttaatata agtatccaac ataccaactt ttctaagaga
     2761 atctgcatat cccaataaat aagaaaactc attgccagaa ttctgagctg atacattctt
     2821 agatagttca agtacattgt cgtattcgtt tattcttttg tgaataaggt tatcgttacc
     2881 ttttaaaggc gagtttttat gttgaagatc aactttctcc ttatgaattg ataataaaaa
     2941 atcaagagca ccaatatttg tccctaattt ctttttacgt tcaactaaat ttatatattc
     3001 tgcgttcata cattcactta atgataaata aaatgagaat aaaactgcat ttactaaata
     3061 ttataaatat aaatctttaa aaacatcact aaaactcaac gaatgtcaca gtgtaagctc
     3121 acagagatat tgcaattatc tccggacaag cgagggaaat gctaagttga tgcaacgtat
     3181 ctgcggcgga tatttgaggc attaccatgc caacatctat gtgatgaaac actttgttat
     3241 acctgagatt acaaaattaa ggatgaaaaa atatacttct gggagaaaca tgagatcaat
     3301 acagttgcgg aagtttttgc tgtatccaca cgaacgctgt attatggccc agaggcacct
     3361 atttcgcgta gcaaagctcc gctagtacat cgcacaaagc aatggcatct cgtaattctg
     3421 taagaaatac gtcgactgag aatagagctt cccaacctag aagaacagat ttttgtttgc
     3481 ttgaagccgt ggagtgaaca gcaccgcctg gtctgtccga gcatttcaac catcggcagg
     3541 atgttcacgg ccgcacacga taaaatgcga ctgataccga tgtgactgcc tccccaagga
     3601 aaaacactgt tcataaaaaa tacaatcctc caacccgcag atcaaaacgc tatcgcccgg
     3661 taaagagcag tgagctcgtt ggtatagatg ctattgagct cagaatgggc gagctgcgct
     3721 gttatgtcat taccatgatc gacgaacaca gcaactacgc tctggcacag gccgtgccgt
     3781 cacttaactg tgatatcatc aaactcttct tttgccgtgc agaccggcta ttccctatcg
     3841 atatcaacca gggcatcgca gataacggga aagagttccc aggaaacttc gacaaagcac
     3901 cgcaggaatc cgctagcaag ccctatggac ctaccccttt acgcctagaa cgcacctctg
     3961 aacgttttaa gcgaacgccg aaagaacagt tcattgaatt taaataaata gactctttaa
     4021 agagctaaac atatttaatc agaaactagc tgaaaaccta gtactatact gcagtaaaag
     4081 acagcatgaa gtgctcgaat tactgacacc agaagaaaat attttacgag agaacaaaat
     4141 ttgcaatatg tggcgaatct atacggtcac ttgcaaaagt ataaattggt attataatgc
     4201 gtgatattat ataaataaaa taactaacta ttttaacgaa agcaaaacac cacaaagaga
     4261 aaaataaaat ttgcaatata tctcattagg gttcacattt ttaacagccc ttgattttag
     4321 ctctcctata tttataaaca acaacaatta acttaaatgg aagggtaatg aatccaaata
     4381 tcgaattagc actggagata atagcctgcc ctaattgata agataaatgt ttcttaactc
     4441 tttcagcctt atcagcatca gcatataaat agagtggcac atcaacgcca taactcttct
     4501 tacgtttttt ttcaaaaaca cactcacgga taagtgcaaa tggcatggta ataatcccct
     4561 tgagggattt agaatacgag actataatat aaccaagtcg ataacctaac tcttgtttta
     4621 tctgctcagc tgctccatat agcacaggtg gaagtttctt tttaatttcc tgattttcta
     4681 taaaatagtg ttccaactgc tcttgcacta tatgcaattg ttttaaaatc tgttcttttt
     4741 ctgatattgc ctgagtgagt tttgattctt tttcgcttat ctcttcttca tatttatgca
     4801 attgttttaa aatctgttct ttttctgata ttgcctgagt gagttttgat tctttttcgc
     4861 ttatctcttc ttcatatttc tgtatgtcat ttattttttt ttcaatatca tctggatatt
     4921 tttcttgtct cacaacatcg ttgtgattta tttttgaaag tttaattacc tcttcctttt
     4981 ctttttttag ctcattaatc ctgttcaata ataaaaattg tcttttctta aatatagatt
     5041 tagtcaattc aatatctgca tccttctttt ttgactcttg caccaaatga gatacaatat
     5101 tgaagataga atttctctct tcgactacct cccataatgc agcctcagcc gaaacgttat
     5161 ctttcgatgt ttctatataa ggtaggtttg cataggcttg taactcatta taaagcttca
     5221 gaacctcggt tctttctttt attaattcag atactaagta ctcactaacc tttgtatcat
     5281 taaatgtaat ttcagtctca tttgcactta aagaaaataa agttgtatat ttttcaagca
     5341 gggcaatttc atttgatttt gtataatcat tattctcaac cacatcaaac aatattgaat
     5401 ttttgtaatg cgcagacatt agattatcta tttttataat attactcaat ggctttataa
     5461 tatttttctt attgctttga aacgcctcaa aattaatcaa cacacatcgt tctttattct
     5521 ccaaaaaaaa atcaagcaat cttttattat atgcaatcca gttatctaca ctattattta
     5581 tatttaaaga gagaggggca ttatttactg aacgcattaa attatattta gggtgatcat
     5641 atatcatgat gaattttatg cttttatcta tatttttcca aaaatcaaga aaaaatatta
     5701 tagatggatc tgcccacccc caaagtttgt tatttagatt gttcatcatt agttcaagaa
     5761 ccatgctatc ccacatggtt gagacttgaa cagctgagaa ttctctctca tctactacat
     5821 cattaacttc tggtgtttga taaaattgac ataatgaagc ggtgatatat tctggggaaa
     5881 ttttattact tgtagaataa tttggtgagt tcatgccaat ttctttcaaa aaatgttcaa
     5941 ctatctgata ctttgagcca ggatgtccga ctagcactaa tttattcatc atatttcctc
     6001 tttattatct acaagattac tttattttaa acactgtcgt ttatcgtaat ggtgatattg
     6061 cagacatata tttcaaatct gtaatgtaat ttaggtggcg ttatgtgata atactaaatg
     6121 aaactatcta tatctcaata gcacatctga tttatttggt atagccattc atcaataaac
     6181 aatatcaata taactcagag cacctcctaa taattgacat attgcgttac caccttcttt
     6241 tagacaaaaa tcttaattat aaactctacc aaactaacgc tgatattttt caaaatcata
     6301 aaacaaatgg tttaaataag tatataacaa aatatcaacg tacaactatt taaaacaagt
     6361 atttatacta taaacttcag aaaccaacac aaaatacagg aatgtgaata aaaaagtaag
     6421 tttttaccct tccacattat acactaattc gaggttaagt tttgaatatc ctgcgattgc
     6481 ttgtgtttct tttatgatgt ctaaaggcca tttttttgta aatgtgttcc aaagtccttc
     6541 cattgaacct gatgagacct ctcttagcca gtttttttct ctgggaacac atatcatacc
     6601 aatttcattc tctaacatat agcgagaaaa tctaacatcg acgaatcgtt gagaaccatc
     6661 catatatttt aaagatggta attgatctgc cttaagaaaa acagtccctg tacctaattg
     6721 atttacaact ctcggtcgca atagcccttg agtaaatgaa aatacttttc ttttagactg
     6781 atctccatca aatgcatcta tgtatataca gccatgaatc ccaacaatgc aattgaatat
     6841 tgcaaaggaa ttataaaaat tgagcatttt ttctacataa tcgggagggt aaataatatc
     6901 atcatctgta agtacgatca tatcattttt agcgcaagga aatataaatt tgcccacatc
     6961 cttataatct ttatctggaa taactggatt taattttgaa aaaccatcta attcctcagg
     7021 aatttcttca aactcattca ggcaaagatt aattttatct acttgagcat gtaaactttg
     7081 tatagaatgc accaactctt tttttcgagg tgggtatgat gacatatttg caacaatcat
     7141 ggggtaatat tttaattaat attgcatgtt aatgaaaact atcaacgtat tgatagtcgt
     7201 ttatttatag gaaatgctta ataaacagaa cacattaagt tgattagaat gactccgcac
     7261 ctcatggacg atatgtttaa ttaaaaataa ctactagggt gtacgtttga tatatttatc
     7321 ttcatctaaa ttattactct taccatatca aaccactctt tgcataaaca tttcctgtaa
     7381 ctatctaaaa ataaattggc agataattaa ttaaagaata acagcaacgt attacgagat
     7441 cacgatcaaa ttcataatta cctagttcaa agtatactgt ccatcctcct catacactaa
     7501 atacaataga gtttatataa ttatatagag tgtgattaca atctccggca atgacactgt
     7561 tagtgcaaca gtaatcaggc ctccctctta gtatcgctta cgctgaaaga ataactggca
     7621 gcctgccaca ccatgttgtc tggcaacgag gaagaccgcc attttcggtt caaggctctg
     7681 ctgaaaaata gcgattttcc tgtgtggcac gctgtctgcg tttctctgct tctaagacac
     7741 caattatcta ttctccaact actagtctaa aagactagta tcactactat cagttattta
     7801 aatgatattg attgcctaga gattcaggga gctagtctag ttattcagag aacggtatct
     7861 taacaatttt gttatgattt taggagatct ggatttctat tcatgatgct tggcgacaaa
     7921 aataaatgaa caaaaagaac ttatatcagc cacagcaaca taatttctta tgtcacttgt
     7981 attctacctg tgtcattaag tttatcgttt caatttcata agata
//
