LOCUS       NZ_DS264579             9403 bp    DNA     linear   CON 25-NOV-2019
DEFINITION  Bacteroides ovatus ATCC 8483 Scfld0226, whole genome shotgun
            sequence.
ACCESSION   NZ_DS264579
VERSION     NZ_DS264579.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00627058
            Assembly: GCF_000154125.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacteroides ovatus ATCC 8483
  ORGANISM  Bacteroides ovatus ATCC 8483
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Bacteroides ovatus (ATCC 8483)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Bacteroides ovatus (ATCC 8483)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9403)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   4  (bases 1 to 9403)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   5  (bases 1 to 9403)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2019 16:35:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 4,931
            CDSs (total)                      :: 4,837
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 3, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 1 of 149
            Pseudo Genes (frameshifted)       :: 75 of 149
            Pseudo Genes (incomplete)         :: 69 of 149
            Pseudo Genes (internal stop)      :: 17 of 149
            Pseudo Genes (multiple problems)  :: 14 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            DS264579.
            Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene:
            X83952) is a member of the division Bacteroidetes. In one
            comprehensive 16S rDNA sequence-based enumeration of the colonic
            microbiota of three healthy adult humans, it represents, on
            average, 0.034% of all 16S rDNA sequences and 0.071% of the
            sequences in its division (Eckburg et. al. (2005)). The sequenced
            strain was obtained from ATCC (ATCC 8483T).
            We have collected 6.9X coverage in plasmid end reads and 454 reads.
            We will be performing one round of automated sequence improvement
            (pre-finishing).
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences.  454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs. These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads.  Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene:
            X83952) is a member of the division Bacteroidetes. In one
            comprehensive 16S rDNA sequence-based enumeration of the colonic
            microbiota of three healthy adult humans, it represents, on
            average, 0.034% of all 16S rDNA sequences and 0.071% of the
            sequences in its division (Eckburg et. al. (2005)). The sequenced
            strain was obtained from ATCC (ATCC 8483T).
            We have collected 6.9X coverage in plasmid end reads and 454 reads.
            We will be performing one round of automated sequence improvement
            (pre-finishing).
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences.  454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs. These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads.  Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were
            blasted against NCBI'snon-redundant (NR) database and predictions
            generated based on proteinalignments. RNA genes were determined
            using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the
            contig level and may notnecessarily reflect any known order or
            orientation betweencontigs.
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Annotation was added to the contigs in August 2007, and the CDS
            comments were updated in January 2008.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Product names were updated in August 2012.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Bacteroides ovatus ATCC 8483"
                     /mol_type="genomic DNA"
                     /strain="ATCC 8483"
                     /isolation_source="biological product [ENVO:02000043]"
                     /host="Homo sapiens"
                     /type_material="type strain of Bacteroides ovatus"
                     /db_xref="ATCC:8483"
                     /db_xref="taxon:411476"
                     /note="type strain of Bacteroides ovatus ATCC 8483"
     gene            1..816
                     /locus_tag="BACOVA_RS11930"
                     /old_locus_tag="BACOVA_02741"
     CDS             1..816
                     /locus_tag="BACOVA_RS11930"
                     /old_locus_tag="BACOVA_02741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH16"
                     /protein_id="WP_004298557.1"
                     /translation="MKKVALFLFLSVCFAQQSCSSDSVGTEPEENPQDILFKDDFNFFD
                     EKVWTKETHEPGWTNQELQAYDAAHVSVGKDGDKSVLILTAERKGNKIYSGRINSKGKK
                     SFKYRKIEASIKLPKTNGGLWPAFWMMGDNDKQWPACGEIDIMEMGEQSGMAAGDSEKQ
                     VNTAIHYGPSAAAHEQQYYKANVANSLQDGNYHTYSLDWDENNLTISIDNVKFHTFDIS
                     SNTYFHDNFYILFNLAVGGAFTGITDINKLTGLKDGQKVNMYIDWVKIL"
     gene            837..4025
                     /locus_tag="BACOVA_RS11935"
                     /old_locus_tag="BACOVA_02742"
     CDS             837..4025
                     /locus_tag="BACOVA_RS11935"
                     /old_locus_tag="BACOVA_02742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q93TH9|1.B.14.6.2"
                     /protein_id="WP_004298558.1"
                     /translation="MNMEKCKYLLFCGTLALWMAGSFQQVYAATEPSSQAIQQQSNKVT
                     GKVSDATGPIIGASVVEKGTANGTITDLDGNFSLNVKAGATLVVSYVGYKSEEVKAGRG
                     PLNITLKEDAKALDEVVVTALGIKRERKALGYGIDEVKGEALTKAKETNLINSMAGRVP
                     GLVVSQTAGGPSGSTRVILRGSTEMTGNNQPLYVVDGVPLDNTNFGSAGTNGGFDLGDG
                     ISSINADDVENMSVLKGPAASALYGSRASHGVILITTKRANKDKISVEYNGTLTFDTQL
                     AKWDEVQQIYGMGSNGTYSYDAISNTNKSWGPKADGSNMLKYFDGVERPFLIVPDNTSN
                     FFRTGITATNSAIIGVNSGKTGIRFTYTDMRNKDIVPQTHMSRDIFNLRANTSAGKVDL
                     DFSVNYTREDVKNRPALGDSKSNIGKNLMTLATTYDQEWLQTYQTADGEYSNWNGMDPY
                     NVNPYWDIYKNFNKSKKDLFRMNGKAVWNIDPHLKLQATLGAELNWFTFDDYKAPTTPG
                     FEAGRLQNSAFRNRMYNFEALALYNNHWGDFDFNATLGGNVYKVNNQTTVTTAQDMKIR
                     DVPSLTSFNEISVVPGSYRKQINSVYGAVNVGWKHMLYLDATLRGDQSSTLPTGNNMYV
                     YPSFSGSFVFSELTKLGDLLPYGKVRMSWAQVGSDTDPYQLGLVYTKSKYAYPGYTIGY
                     IDNGTIPNKDLKPTKTNSVEMGLELKFLKNRIGLDFTYYSQISKNQIMGMASSWTTGYN
                     YRLINAGKIENKGIEIALSTRPIQTRDFSWDINLNFSKNSNKVKELDGESDMFELEKAS
                     WLDVQVAAKVGENFGSIVGPDFQRNEKGDILIDPQTGLPQYDKSNHVLGNASWDWTGGL
                     LTNFTYKNLSLVAVFDVKVGADLYSMSARAAYESGKSPETLAGRDAWYRSEEERLAAGI
                     AKGADNWKPTGGFVAPGVIDNGDGTYRPNDIYINPEDYWMSVCRNAPSMFIYDNSYVKC
                     RELTLSYNVPKSWLKNVVSGLTVSFVARNPFIVWKNIPNIDPDSNYNNTTGMGLEYGSL
                     PSRRSYGFNVNVKF"
     gene            4037..5713
                     /locus_tag="BACOVA_RS11940"
                     /old_locus_tag="BACOVA_02743"
     CDS             4037..5713
                     /locus_tag="BACOVA_RS11940"
                     /old_locus_tag="BACOVA_02743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8A0N7|8.A.46.2.2"
                     /protein_id="WP_004298559.1"
                     /translation="MRQSKYITIITMACALFFASCSDEYMENMNTDPSKAATIDPNAQL
                     TTAQLQTYGDLSMMEIYRNYHYAFTQQLMGCWNTTNYGGRHTLDNNEMSRIWTSFYTQS
                     LKNIIDAQYRTAEDAEKVNINSVLRIYRVYLMSIITDTYGDAPFSEAGLGFLEGKFNPK
                     YDKQEDIYNAFFLELEDAVNKIDPTKDKVTGDLIYAGDVTKWQQLANSLRLRFAMRISS
                     VNPTKAQTEFENALAANGGVITDASSDALIKYMTIAFSFGQEAYSDYRGNSLSQLLFGN
                     DPANNPSYLCSTFFNQLYNSGDPRTFKISRCYYDGLMSATSPDNRVDITQEMIEKGIAF
                     SPRDPGAYSWEPWPTGYDSDICAELAVNNPSVTATMAREVEPKLANNFLKSDNPGVVMT
                     SAEVKFLMAEATVKKWNVGSVSAEDLYKQGVRAAIDFLTDNYGCTATTDAEFDAFIQDK
                     GAFGHTDNQKLEAINTQAWILHFTNPAECWANVRRSGYPKLKSPAEYGFGQYLTGGTEI
                     PVRLCYPVLESSYNKKSYNEAIERMGGTDNWHSLLWWDTEN"
     gene            5734..6996
                     /locus_tag="BACOVA_RS11945"
                     /old_locus_tag="BACOVA_02744"
     CDS             5734..6996
                     /locus_tag="BACOVA_RS11945"
                     /old_locus_tag="BACOVA_02744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004298560.1"
                     /translation="MKKIYIALFALLAVGSTFTACTEEEPFATVTENDDPHILAPVFPD
                     RTNGQLATFANISRDANLSIALTVTPKDYTTVTWFIDGQEVESGTDSDKEINRSLKAGT
                     YNLKIEVETVKGKKTSREGLVVVNPLADDPQSKEVAFERIVSPGKTARLYGSNLQNVTA
                     ILLGGNTITDPTYVESADENYLEYTIPTGVSEGDYRIVLQDADGNQYGADMVKVTNASL
                     VISGANRATANVDWTISGINLENIASLTIGGQTVSQFSNQSSTEITLTCPDLSDGSYTM
                     TGKTRSGEAVQFLNDNITTTEQTVTVSTEITLWSGHHYVSWDKPDGDPNKTFGLIPMDV
                     FAGITAGSTLKVVYSIEPTAEYHKMQLATGYWTGLASEMEFTENGEYTLILTQDMLNKI
                     QAEAGFLCVGHGYYVDLVTVK"
     gene            7109..9403
                     /locus_tag="BACOVA_RS11950"
                     /old_locus_tag="BACOVA_02745"
     CDS             7109..9403
                     /locus_tag="BACOVA_RS11950"
                     /old_locus_tag="BACOVA_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3"
                     /protein_id="WP_004298561.1"
                     /translation="MKLRNIILMTASIAMTACSKQAVPTAIPEDAKIEQQVEELLSKMD
                     LDAKIGQMTELAIDVLGETINGEFQLDEAKLHKAIAEYKVGSFLNAPGPVAQSPEKWNE
                     IIGRIQELSMAEIGIPCIYGLDQNHGTTYTLGGTLFPQNINMGAAFNPDLTYEAARVTA
                     YETRASNCPWTYSPTVDMARDPRWPRVWENYGEDCLVNAIMGSTAVRGFQGDDPNHIPA
                     DRIATSVKHYMGYSMPRTGKDRTPAYISVSELREKCFAPFRACVEAGALTIMVNSGSIN
                     GKPVHADRELLTQWLKEDLGWDGMLITDWADINNLYTREHVAANKKEAIEMAINAGIDM
                     AMEPYDLNYCTLLKELVQEKKIPMSRIDDAVRRVLRLKFRLGLFDHPNTLLKDYPLFGS
                     KEHALIALHAAEESEVLLKNKDNILPLPQGKKLLVTGPNANSMRCLNGGWSYSWQGHLT
                     DRFADKYNTIYEAICNKFGADHVRLEQGVTYKPEGAYMEENEPEIEKAVAAARNVDIII
                     ACIGENSYCETPGNLSELAISASQSKLVKALAATGKPIILILNEGRPRIINELEPLADA
                     VIDILLPGNYGGDALANILAGDVNPSAKMPYTYPRHEAALTTYDYRVSEEMDKMEGAYD
                     YNAVVSVQWPFGYGLSYTTFEYSNFQTDKSSFTAGDDLHFSIDVTNTGKYAGKEVVMLF
                     SSDLVASLTPENRRLRAFKKVELQPGETQTITLSIKGSDLAFVNSDGQWVLEQGDFRMQ
                     CGNQTLNIICK"
ORIGIN
        1 atgaaaaaag tagcactatt cctattttta tccgtttgct tcgcgcaaca atcctgttcg
       61 tcggattctg ttggaacgga accggaagaa aatccacaag acattctttt taaagacgat
      121 ttcaactttt tcgacgaaaa agtatggacg aaagaaactc atgaacccgg ttggaccaac
      181 caagagctac aggcatacga tgccgctcat gtatcggtag gaaaagacgg ggataaatcg
      241 gttctgattc ttaccgccga acgcaaaggg aataaaatct attcaggcag gatcaattca
      301 aaaggaaaaa agagtttcaa gtatcgcaag atagaagcaa gcatcaagct tcccaagact
      361 aacggcggac tatggcctgc tttctggatg atgggagaca acgacaaaca gtggccggca
      421 tgcggggaga ttgatattat ggaaatggga gaacagagcg gaatggctgc gggcgattcg
      481 gaaaaacaag tgaacaccgc cattcactat ggccccagcg cagcggctca cgaacagcaa
      541 tactacaaag ccaatgttgc caacagccta caagacggca attatcacac ctactccctg
      601 gattgggatg aaaacaacct gacaatatcc attgacaacg tcaagtttca tacgttcgac
      661 attagctcga acacatactt tcatgacaac ttctacatcc tgttcaacct cgccgtggga
      721 ggtgcgttta caggaattac cgatattaat aaactgacgg gcctgaaaga cggtcagaaa
      781 gtgaatatgt acatcgactg ggtgaaaata ttataggaat cttaaatata catgctatga
      841 atatggaaaa atgtaagtat ctactgtttt gcggaacact cgccctttgg atggcgggaa
      901 gtttccagca agtgtatgca gcaacggaac caagttccca agctattcaa caacaaagca
      961 acaaggtaac cggaaaagtg agcgatgcca caggtcccat cattggggct tccgtagtgg
     1021 agaaaggaac tgccaacgga acgattaccg atctggatgg aaatttcagt ttgaatgtaa
     1081 aagcgggagc tacgctcgtt gtcagctacg tgggttataa atccgaagaa gtaaaagcgg
     1141 gaagaggtcc tttgaacatc accttgaaag aggatgccaa agcattggat gaagtggtag
     1201 ttaccgccct tggcatcaag agagagcgca aagcgttggg ctacggcatt gatgaagtga
     1261 aaggtgaagc cttgaccaaa gcgaaagaga cgaatctcat caactccatg gcaggacgtg
     1321 tgccaggctt ggtagtcagc cagaccgccg gtggtccttc cggttctacg cgcgttattc
     1381 tgcgaggcag taccgaaatg accggcaata accagcctct ttatgtagtg gacggtgtac
     1441 ctttggacaa cactaatttc ggcagtgccg gcacaaacgg aggcttcgac ttgggcgacg
     1501 gtatttcgag catcaatgcc gatgacgtag aaaacatgtc ggtattgaaa ggccctgcgg
     1561 catctgcact ttatggtagc cgtgccagcc acggtgttat cttgattaca accaagcgag
     1621 ccaacaagga taaaatcagc gtggaatata acggaacgct gactttcgac acccaacttg
     1681 ccaaatggga tgaagtgcaa cagatatatg gtatgggaag caacggtact tatagctatg
     1741 atgccatatc caacacaaac aaaagttggg gccccaaagc ggacggttca aacatgttga
     1801 agtatttcga cggcgtagaa cgccctttcc tcattgtccc cgacaatacg tccaacttct
     1861 tccgcacagg tatcaccgcc accaactccg ccatcatagg tgtgaacagc ggaaaaaccg
     1921 gaattcgctt tacctatacg gatatgcgca ataaagacat tgttccccag acgcacatga
     1981 gccgtgacat tttcaacctg cgcgccaata cgtctgcagg aaaggtagac ttggatttca
     2041 gtgtcaacta tacacgggaa gacgtgaaga accgtccggc tctgggcgac agcaagtcca
     2101 atatcggtaa aaacctgatg acccttgcca ccacctacga ccaggaatgg ttgcaaacct
     2161 atcagactgc cgacggcgaa tattccaact ggaacggcat ggatccctac aacgtgaatc
     2221 cgtactggga tatctataag aacttcaaca aatccaagaa agacttgttc cgcatgaacg
     2281 gcaaggccgt atggaacatc gacccacatc tgaaacttca ggcaacgctg ggtgccgaac
     2341 tcaactggtt cacgttcgat gattacaaag cacctaccac tcccggattc gaagccggaa
     2401 gacttcaaaa cagtgctttc cgcaaccgca tgtacaactt tgaagcgttg gctctctaca
     2461 acaaccattg gggtgatttc gatttcaatg ctacattagg cggcaatgta tataaggtga
     2521 acaaccagac caccgttacc accgctcaag acatgaagat acgcgatgtc ccttcattga
     2581 cgagcttcaa cgagatcagt gtagtgcccg gcagttaccg caagcagatt aattcggttt
     2641 acggagcagt gaacgtggga tggaaacaca tgctttacct cgatgctacg ttgcgtggcg
     2701 accaatcgtc tacccttccc actggcaaca acatgtatgt gtatccttct ttctccggta
     2761 gctttgtatt ctccgagctg acaaaactgg gcgacctcct gccttacgga aaggtgcgta
     2821 tgtcatgggc ccaagtaggt agcgataccg atccttatca attgggactc gtctatacga
     2881 aatccaaata tgcttatccc ggatatacca tcggatatat cgataacgga accatcccca
     2941 acaaagactt gaaaccaacc aaaacaaact ccgtggaaat gggattggaa ttgaaattcc
     3001 tgaagaaccg tatcggactg gatttcacct actattctca aatcagtaaa aaccagatta
     3061 tgggaatggc aagttcctgg acaaccggtt acaactatcg cttgattaat gccggcaaga
     3121 tagaaaacaa aggtattgag attgccctta gcacacgacc gattcaaaca cgcgattttt
     3181 cctgggacat taatctgaac ttctccaaaa acagcaacaa agtaaaagaa ctggatggcg
     3241 aatcagatat gtttgaactg gaaaaggcgt cttggctcga cgttcaggtg gccgctaagg
     3301 taggcgagaa ctttggttct attgtaggcc cggacttcca acggaacgaa aagggagata
     3361 ttctgattga cccgcaaacc ggtctgccgc aatatgacaa gagcaaccat gtattaggta
     3421 atgcctcttg ggactggaca ggtggattac tcaccaactt tacctacaag aatctctcgc
     3481 tggtagcagt attcgatgtg aaagtaggcg ccgacctcta ttctatgtct gcccgtgctg
     3541 cttacgaatc gggtaaaagt ccggaaacat tggcaggacg cgacgcctgg tatcgctcgg
     3601 aagaagaaag attggcagcc ggcattgcaa aaggagccga taactggaag ccgacaggcg
     3661 gattcgttgc tccgggtgtc atcgacaacg gtgatggaac atatcgtccc aacgacatct
     3721 acatcaatcc ggaagactac tggatgagcg tttgccgtaa tgcaccttct atgtttattt
     3781 acgacaactc atacgtgaaa tgccgtgagc tgacgttgag ttacaatgta cccaaatcct
     3841 ggctgaagaa tgtagtaagc ggactgaccg tttcgttcgt tgcccgcaat ccgttcatcg
     3901 tatggaagaa tatcccgaat atcgacccgg attccaacta caacaatacc accggtatgg
     3961 gacttgaata cggttcgttg ccttcacgca gaagctatgg ttttaatgta aatgtgaaat
     4021 tctaaaaaga tatatcatga gacaatctaa atacatcaca atcatcacga tggcatgtgc
     4081 cctgtttttc gcttcttgca gcgatgaata catggaaaac atgaataccg acccttccaa
     4141 ggccgctacc atcgacccga atgcgcagct caccactgcc caactgcaaa cgtacggaga
     4201 ccttagtatg atggaaatct accgtaacta ccattatgca ttcacccaac aactgatggg
     4261 atgttggaac accaccaact acggcggacg ccatacgcta gacaataatg aaatgagccg
     4321 tatctggaca tcgttctaca ctcaatccct gaagaatatc attgatgctc aatatcgcac
     4381 agccgaagat gcagagaaag tgaatatcaa ctctgtactc cgcatttatc gggtttatct
     4441 tatgtctatc atcaccgaca cttacggaga cgctcctttc agtgaagccg gcctgggatt
     4501 cctcgaagga aagttcaatc cgaagtatga caaacaggaa gatatttaca acgctttctt
     4561 tctggagctg gaagatgccg tcaataaaat cgatccgacc aaagataaag tgacaggtga
     4621 cctcatctat gccggtgatg taactaaatg gcaacaactg gcaaactcgc ttcgcctacg
     4681 ttttgccatg cgaatatcca gtgtcaatcc tacaaaggca caaacggaat ttgaaaacgc
     4741 tttggctgcc aatgggggtg tgataacgga cgcctcaagc gatgcgctca tcaaatacat
     4801 gacaatcgca tttagtttcg gacaagaagc ttactctgat tatcgcggaa attcattgtc
     4861 acagttgttg ttcggcaacg accccgctaa taatccaagt tacctctgtt ccactttctt
     4921 taatcagtta tacaattccg gtgacccccg tacattcaaa atttcccgtt gttattatga
     4981 cgggctgatg agtgccacca gtcccgacaa tcgtgtcgac atcacccaag agatgattga
     5041 aaaagggatt gctttcagtc cgcgcgaccc aggtgcctac tcgtgggaac catggcctac
     5101 agggtacgac agcgatattt gtgcggaatt agccgttaac aacccttccg ttacagcaac
     5161 catggctcgt gaagtggaac ccaaacttgc caataatttc ctaaaaagcg acaatccggg
     5221 cgtagtgatg acctctgcag aagtgaagtt cctgatggcg gaagctaccg tgaaaaaatg
     5281 gaatgtcggc agtgtgtcag cagaagatct ttacaagcaa ggagtacgtg ctgctattga
     5341 tttcctgaca gacaactacg gttgcacggc tactactgat gcagagtttg atgcattcat
     5401 tcaagataaa ggggcgttcg gacatacgga caatcagaaa cttgaagcta tcaatacaca
     5461 ggcctggatt cttcacttca ccaatcctgc cgaatgttgg gcaaacgtgc gtcgttccgg
     5521 ttatccgaag ttgaaatcac cggcagaata tgggttcgga caatacctca ccggtggaac
     5581 ggaaattccg gtacgtctat gctatcctgt attggaatct tcctataata aaaagagcta
     5641 caatgaagcc atcgagcgca tgggaggaac agacaattgg cacagccttc tgtggtggga
     5701 tactgaaaac taactattaa acaatcatcc attatgaaaa agatatatat tgcactattt
     5761 gccctactcg ctgtaggctc cacatttact gcatgcaccg aggaagaacc gttcgctacg
     5821 gtgacagaaa atgatgatcc gcatatcctt gccccggtat tccctgacag aacgaacggc
     5881 caattggcta cattcgccaa catcagtcgg gatgcgaact tatccatcgc gctgaccgtg
     5941 actccgaaag attacaccac agtaacctgg ttcattgatg ggcaggaagt agaatcgggt
     6001 accgactccg acaaagaaat caaccgtagt ctgaaagccg gtacgtataa tttgaaaata
     6061 gaagtggaaa ctgtgaaagg gaagaagact tcccgtgaag gattggtggt ggtcaatccg
     6121 ttggctgatg accctcaatc gaaagaagta gcctttgaga gaattgtatc tcccggtaag
     6181 actgcccgtt tatatggcag taatttacag aacgtaacag ccatcctctt gggaggaaac
     6241 acgatcaccg atcctactta tgttgaatcg gcagacgaga attatctgga atacaccatt
     6301 ccgacaggcg taagcgaagg cgattaccgt atcgttctgc aagatgccga cggcaatcaa
     6361 tatggagccg acatggtgaa agtaaccaat gcttcccttg tcatttcggg agctaaccgg
     6421 gctactgcca acgtggattg gaccatttcc ggcatcaatc tcgaaaacat cgcttctcta
     6481 accattggcg ggcaaacggt atctcaattc agcaaccaat cttctacaga gattacgctg
     6541 acttgtccgg atttgtctga tggaagctat acgatgacag gcaagacccg cagcggagag
     6601 gctgtgcagt tcttgaacga taatattacg actaccgaac agactgtgac cgtatctacc
     6661 gaaataacgc tttggtcagg acaccattac gtttcgtggg ataagccgga cggtgatccc
     6721 aacaaaactt ttggtttgat tccaatggat gtatttgctg gtatcacagc cgggtcgaca
     6781 ctgaaagtag tctactccat agaacctact gccgaatatc ataaaatgca gcttgccaca
     6841 ggctattgga caggcttggc aagtgagatg gaatttacgg aaaacggtga atatacactg
     6901 atactaacac aagacatgtt gaataagatt caagctgaag ccggattctt atgtgtagga
     6961 catggctatt atgtagattt ggtaaccgtg aaataatgtt cctaagtaag tttaaaaatc
     7021 attcatacca taagtatctg tcattcttcg ggttaaagcc ctctgaatga cagattacat
     7081 aaacgatgct acttaaaaac aaatcataat gaaactaaga aatattatat taatgaccgc
     7141 atctatcgca atgactgcct gttccaaaca ggcagttccc actgccattc ctgaagacgc
     7201 taaaatagaa caacaagtgg aagagcttct atcaaagatg gatcttgacg ccaagatagg
     7261 gcaaatgacc gagcttgcta ttgatgtatt aggagagacg ataaatggag aattccaatt
     7321 ggacgaggca aaactccaca aagcgattgc tgaatacaag gtaggttcgt ttctcaatgc
     7381 tcccggcccg gtagcacaaa gccctgaaaa gtggaacgag attatcggtc gaatacaaga
     7441 actgtcgatg gcggagattg gtattccatg tatctacggc ctggatcaga atcatggtac
     7501 tacctataca ttgggcggca cgctctttcc tcaaaatatc aatatgggtg ctgccttcaa
     7561 tccggatttg acctacgaag cggcacgtgt aacggcttat gaaacaaggg ctagcaattg
     7621 tccctggaca tactccccca ctgtcgatat ggcacgtgat ccccgttggc cgcgtgtatg
     7681 ggaaaattat ggagaagact gtttagtcaa tgccattatg ggcagcactg ctgtccgagg
     7741 cttccaagga gatgatccta accatattcc cgccgaccga atcgccacct ccgtgaaaca
     7801 ttacatggga tacagcatgc cacgtaccgg caaggaccgc acaccggctt atatatctgt
     7861 ttccgaactg cgtgaaaaat gttttgcacc gttcagggct tgtgtggaag caggtgcact
     7921 gactataatg gtcaacagtg gttccatcaa cggaaagcct gtgcacgccg accgcgaact
     7981 ccttacacaa tggctgaaag aagacttagg atgggacgga atgttaatca ccgactgggc
     8041 ggacatcaac aatctttata cccgcgaaca tgtggcagca aacaagaaag aagccatcga
     8101 aatggcaatc aatgcaggta tagacatggc tatggaaccg tatgatctca attattgtac
     8161 actactaaaa gagttagtgc aagagaagaa aatacccatg agccgtattg acgatgccgt
     8221 ccgccgggtg cttagactta aattccgtct gggtctgttc gatcatccga acactttatt
     8281 gaaagattat cctctcttcg gcagcaagga gcatgcactt atcgcacttc atgcagctga
     8341 agaatcggaa gtattgctaa aaaataaaga taatattctt ccgcttcctc aagggaaaaa
     8401 gctactggta accggtccga acgccaactc tatgcgttgc ttgaacggcg gttggagcta
     8461 ttcgtggcaa ggacatctga ccgatagatt tgccgacaaa tataacacca tttacgaagc
     8521 aatctgcaac aaattcggag ccgatcatgt ccgtctggaa cagggcgtaa cctacaaacc
     8581 cgaaggtgct tacatggaag aaaatgaacc ggagattgag aaagctgtgg ccgccgcccg
     8641 caacgtggat attattatcg cctgcatcgg tgagaattca tattgcgaaa cacccggtaa
     8701 tctttccgaa cttgccatct ctgccagcca aagcaaactg gtaaaggcac ttgccgctac
     8761 aggaaaaccg atcatcctga tactcaatga gggtcgtccc cgcattatca acgagttgga
     8821 gccattggct gatgcagtta ttgacattct attacccgga aactatggtg gcgacgcatt
     8881 ggcgaacatt ctggcaggag acgtgaaccc cagtgccaag atgccttaca catatccgcg
     8941 acacgaagca gcactgacta cctacgatta tcgggtgagt gaagaaatgg acaaaatgga
     9001 aggagcttat gactacaatg cagtagtctc cgttcaatgg cctttcggat acggattgag
     9061 ttacaccact ttcgagtaca gcaacttcca aacggacaaa tcttcgttta ccgcaggaga
     9121 tgatttgcac ttttccatag atgtaaccaa taccggtaaa tatgccggaa aagaagttgt
     9181 catgctattc agcagtgatc ttgtggcttc gcttactccc gaaaaccgcc ggttacgagc
     9241 cttcaaaaag gtggaactgc aaccgggcga aacccagact atcactctat ccatcaaagg
     9301 cagcgacttg gcttttgtca attccgacgg gcaatgggta ctggaacaag gggatttccg
     9361 tatgcaatgc ggaaatcaaa cactgaatat tatatgcaag taa
//
