LOCUS       ABCLSDA                 3410 bp    DNA     linear   BCT 24-JUL-2016
DEFINITION  Gluconacetobacter diazotrophicus levansucrase precursor (lsdA),
            levanase precursor (lsdB), pseudopilin G precursor (lsdG), prepilin
            peptidase and N-methyltransferase O (gspO), kinase E (lsdE), LsdF
            (lsdF), pseudopilin H precursor (lsdH), pseudopilin I precursor
            (lsdI), pseudopilin J precursor (lsdJ), LsdL (lsdL), LsdM (lsdM),
            and LsdN (lsdN) genes, complete cds; secretin D (lsdD) gene, partial
            cds; and unknown gene.
ACCESSION   L41732
VERSION     L41732.5
KEYWORDS    .
SOURCE      Gluconacetobacter diazotrophicus
  ORGANISM  Gluconacetobacter diazotrophicus
            Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
            Acetobacteraceae; Gluconacetobacter.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Arrieta,J., Hernandez,L., Coego,A., Suarez,V., Balmori,E.,
            Menendez,C., Petit-Glatron,M.F., Chambert,R. and Selman-Housein,G.
  TITLE     Molecular characterization of the levansucrase gene from the
            endophytic sugarcane bacterium Acetobacter diazotrophicus SRT4
  JOURNAL   Microbiology (Reading, Engl.) 142 (PT 5), 1077-1085 (1996)
   PUBMED   8704949
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Hernandez,L., Arrieta,J., Betancourt,L., Falcon,V., Madrazo,J.,
            Coego,A. and Menendez,C.
  TITLE     Levansucrase from Acetobacter diazotrophicus SRT4 is secreted via
            periplasm by a signal-peptide-dependent pathway
  JOURNAL   Curr. Microbiol. 39 (3), 146-152 (1999)
   PUBMED   10441728
REFERENCE   3  (bases 1 to 3410)
  AUTHORS   Menendez,C., Hernandez,L., Selman,G., Mendoza,M.F., Hevia,P.,
            Sotolongo,M. and Arrieta,J.G.
  TITLE     Molecular cloning and expression in Escherichia coli of an
            exo-levanase gene from the endophytic bacterium Gluconacetobacter
            diazotrophicus SRT4
  JOURNAL   Curr. Microbiol. 45 (1), 5-12 (2002)
   PUBMED   12029520
REFERENCE   4  (bases 1 to 3410)
  AUTHORS   Arrieta,J.G., Sotolongo,M., Menendez,C., Alfonso,D., Trujillo,L.E.,
            Soto,M., Ramirez,R. and Hernandez,L.
  TITLE     A type II protein secretory pathway required for levansucrase
            secretion by Gluconacetobacter diazotrophicus
  JOURNAL   J. Bacteriol. 186 (15), 5031-5039 (2004)
   PUBMED   15262940
REFERENCE   5  (bases 1 to 3410)
  AUTHORS   Menendez,C., Banguela,A., Caballero-Mellado,J. and Hernandez,L.
  TITLE     Transcriptional regulation and signal-peptide-dependent secretion of
            exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus
  JOURNAL   Appl. Environ. Microbiol. 75 (6), 1782-1785 (2009)
   PUBMED   19139238
REFERENCE   6  (bases 1 to 3410)
  AUTHORS   Hernandez,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-1995) Center for Genetic Engineering and
            Biotechnology, Box 6162, Havana 10600, Cuba
REFERENCE   7  (bases 1 to 3410)
  AUTHORS   Hernandez,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-1999) Center for Genetic Engineering and
            Biotechnology, Box 6162, Havana 10600, Cuba
  REMARK    Sequence update by submitter
REFERENCE   8  (bases 1 to 3410)
  AUTHORS   Arrieta Sosa,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2001) Plant-Microorganism Interactions Lab, Plant
            Division, Center for Genetic Engineering and Biotechnology, Box
            6162, Havana 10600, Cuba
  REMARK    Sequence update by submitter
REFERENCE   9  (bases 1 to 3410)
  AUTHORS   Arrieta Sosa,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2002) Plant-Microorganism Interactions Lab, Plant
            Division, Center for Genetic Engineering and Biotechnology, Box
            6162, Havana 10600, Cuba
  REMARK    Sequence update by submitter
REFERENCE   10  (bases 1 to 3410)
  AUTHORS   Hernandez,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2009) Plant-Microorganism Interactions Lab, Plant
            Division, Center for Genetic Engineering and Biotechnology, Box
            6162, Havana 10600, Cuba
  REMARK    Sequence update by submitter
COMMENT     On Mar 13, 2009 this sequence version replaced L41732.4.
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Gluconacetobacter diazotrophicus"
                     /mol_type="genomic DNA"
                     /strain="SRT4; CBS 550.94"
                     /db_xref="taxon:33996"
                     /clone="pALS10; pALS32; p21R1; pALS119; pALS143; pALS152"
     gene            <1..1797
                     /gene="lsdA"
     CDS             1..1755
                     /gene="lsdA"
                     /EC_number="2.4.1.10"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /codon_start=1
                     /transl_table=11
                     /product="GH68"
                     /protein_id="AAB36606.1"
                     /translation="MAHVRRKVATLNMALAGSLLMVLGAQSALAQGNFSRQEAARMAHR
                     PGVMPRGGPLFPGRSLAGVPGFPLPSIHTQQAYDPQSDFTARWTRADALQIKAHSDATV
                     AAGQNSLPAQLTMPNIPADFPVINPDVWVWDTWTLIDKHADQFSYNGWEVIFCLTADPN
                     AGYGFDDRHVHARIGFFYRRAGIPASRRPVNGGWTYGGHLFPDGASAQVYAGQTYTNQA
                     EWSGSSRLMQIHGNTVSVFYTDVAFNRDANANNITPPQAIITQTLGRIHADFNHVWFTG
                     FTAHTPLLQPDGVLYQNGAQNEFFNFRDPFTFEDPKHPGVNYMVFEGNTAGQRGVANCT
                     EADLGFRPNDPNAETLQEVLDSGAYYQKANIGLAIATDSTLSKWKFLSPLISANCVNDQ
                     TERPQVYLHNGKYYIFTISHRTTFAAGVDGPDGVYGFVGDGIRSDFQPMNYGSGLTMGN
                     PTDLNTAAGTDFDPSPDQNPRAFQSYSHYVMPGGLVESFIDTVENRRGGTLAPTVRVRI
                     AQNASAVDLRYGNGGLGGYGDIPANRADVNIAGFIQDLFGQPTSGLAAQASTNNAQVLA
                     QVRQFLNQ"
     sig_peptide     1..90
                     /gene="lsdA"
     mat_peptide     91..1752
                     /gene="lsdA"
                     /product="levansucrase"
     regulatory      1767..1797
                     /regulatory_class="terminator"
                     /gene="lsdA"
     gene            1806..3410
                     /gene="lsdB"
     CDS             1806..3410
                     /gene="lsdB"
                     /EC_number="3.2.1.65"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /codon_start=1
                     /transl_table=11
                     /product="GH32"
                     /protein_id="AAF16405.1"
                     /translation="MTMTGTVPRSAGWRRLSRRCLPAAFALLFPLGAPHAADTPQWRPV
                     LHFSPAAYWMNDPNGPILLDGVYHLFYQYAPGSMTWGHPSWGHATSTDLLHWTEHGVAI
                     AATPGEEIFSGSLVPDPLNRSGLGSTDAPPLLAFHTSVFHDNPAHPDGTQAQSVSVSHD
                     GGFTWRPYAHNPVLTLHPDSRQFRDPSVFWYQDGGCWIMTTVVGDAQLVKLYRSTDLLH
                     WSFLSDFQPSGYRKPGMLWEMPTLVPLKLDGNPRATRWVMIVSVNPWSIAGGSGVQYFV
                     GRFDGVTFTPDALPPPGSDPSRYDWLDHGADQYATTLFANTGSGAPVLIGWMDNWDYAT
                     DLPTAPWRGQMTLPVDIALKTVDGHPTVIQTPTRAYEDMVRKKGVVTYGDRNLPQDGRV
                     TIPSRGEVLDIRLVLRRGGARRAGIVVRETPDGRTGTSVSYDFVDGTLTVDRGRSGLVG
                     FSPRFSTRHIAYLAAPGGEVALHLVVDRASVELFANDGVLRMTDLIFPPAGSDRISLFA
                     EGGGATIHGLRVAVLAR"
     sig_peptide     1806..1913
                     /gene="lsdB"
     mat_peptide     1914..3407
                     /gene="lsdB"
                     /product="levanase"
ORIGIN
        1 atggcgcatg tacgccgaaa agtagccacg ctgaatatgg cgttggccgg gtccctgctc
       61 atggtgctgg gcgcgcaaag tgcgctggcg caagggaatt tcagccggca ggaagccgcg
      121 cgcatggcgc accgtccggg tgtgatgcct cgtggcggcc cgctcttccc cgggcggtcg
      181 ctggccgggg tgccgggctt cccgctgccc agcattcata cgcagcaggc gtatgacccg
      241 cagtcggact ttaccgcccg ctggacacgt gccgacgcat tgcagatcaa ggcgcattcg
      301 gatgcgacgg tcgcggccgg gcagaattcc ctgccggcgc aactgaccat gccgaacatc
      361 ccggcggact tcccggtgat caatccggat gtctgggtct gggatacctg gaccctgatc
      421 gacaagcacg ccgatcagtt cagctataac ggctgggaag tcattttctg cctgacggcc
      481 gaccccaatg ccggatacgg tttcgacgac cgccacgtgc atgcccgcat cggcttcttc
      541 tatcgtcgcg cgggtattcc cgccagccgg cggccggtga atggcggctg gacctatggc
      601 ggccatctct tccccgacgg agccagcgcg caggtctacg ccggccagac ctacacgaac
      661 caggcggaat ggtccggttc gtcgcgtctg atgcagatac atggcaatac cgtatcggtc
      721 ttctataccg acgtggcgtt caaccgtgac gccaacgcca acaacatcac cccgccgcag
      781 gccatcatca cccagaccct ggggcggatc cacgccgact tcaaccatgt ctggttcacg
      841 ggcttcaccg cccacacgcc gctgctgcag cccgacggcg tgctgtatca gaacggtgcg
      901 cagaacgaat tcttcaattt ccgcgatccg ttcaccttcg aggacccgaa gcatcccggc
      961 gtgaactaca tggtgttcga gggcaatacc gcgggccagc gtggcgtcgc caactgcacc
     1021 gaggccgatc tgggcttccg cccgaacgat cccaatgcgg aaaccctgca ggaagtcctg
     1081 gatagcgggg cctattacca gaaggccaat atcggcctgg ccatcgccac ggattcgacc
     1141 ctgtcgaaat ggaagttcct gtcgccgctg atttcggcca actgcgtcaa tgaccagacc
     1201 gaacggccgc aggtgtacct ccataacgga aaatactata tcttcaccat cagccaccgc
     1261 acgaccttcg cggccggtgt cgatggaccg gacggcgtct acggcttcgt gggtgacggc
     1321 atccgcagtg acttccagcc gatgaactat ggcagcggcc tgacgatggg caatccgacc
     1381 gacctcaaca cggcggcagg cacggatttc gatcccagcc cggaccagaa cccgcgggcc
     1441 ttccagtcct attcgcacta cgtcatgccg gggggactgg ttgaatcgtt catcgacacg
     1501 gtggaaaacc gtcgcggggg taccctggcg cccacggtcc gggtgcgcat cgcccagaac
     1561 gcgtccgcgg tcgacctgcg gtacggcaat ggcggcctgg gcggctatgg cgatattccg
     1621 gccaaccgcg cggacgtgaa catcgccggc ttcatccagg atctgttcgg ccagcccacg
     1681 tcgggtctgg cggcgcaggc gtccaccaac aatgcccagg tgctggcgca ggttcgccaa
     1741 ttcctgaacc agtaatgacg gggttcgacc agcccgccct ccggcgggct ggtctttcga
     1801 tattgatgac catgaccggg acagttccgc gatcggccgg atggcgccgc ctgtcgcggc
     1861 gctgcctgcc ggccgcgttc gccctgctgt ttcctctcgg ggcgccgcac gcggccgata
     1921 cgccccaatg gcggcccgtc ctgcatttct cgccggcggc gtactggatg aacgatccga
     1981 acgggcccat ccttctcgat ggcgtctatc acctgttcta ccagtacgct cccggcagca
     2041 tgacatgggg gcatccgtcc tgggggcacg cgaccagcac ggatctgctc cactggacgg
     2101 agcatggcgt cgcgatcgcg gcgacaccgg gcgaggagat cttttccgga agcctggtgc
     2161 cggacccgct gaatcgctcg ggtctgggga gtacggacgc gccgccgctg cttgccttcc
     2221 acacatccgt gttccacgac aatccggccc atcccgacgg tacgcaggca caatcggtgt
     2281 ccgtcagcca tgacggcggt ttcacgtggc ggccgtacgc ccacaacccc gtcctgacgc
     2341 ttcatccgga ctcccggcag ttcagggacc cttcggtctt ctggtaccag gatggcggct
     2401 gctggatcat gacgaccgtc gtgggcgacg cccagctggt caagctctac cggtccaccg
     2461 acctgctgca ctggtcgttt ctcagcgact tccagccctc cggctatcgc aagcccggca
     2521 tgctgtggga aatgcccaca ctggttccgc tgaagctgga cggaaacccg cgggcgacca
     2581 gatgggtcat gatcgtcagc gtcaatccgt ggagtatcgc gggcggatcg ggcgtgcagt
     2641 atttcgtggg ccggttcgac ggcgtgacct tcacgccgga cgcgctgcct ccgccggggt
     2701 ccgatccgtc ccgctatgac tggctcgacc acggcgccga ccagtatgcg acgaccctgt
     2761 tcgccaatac cggctccggc gccccggtgc tgatcgggtg gatggacaac tgggactacg
     2821 cgacggacct tccgaccgcg ccctggcggg ggcagatgac cctgccggtg gacatcgcgc
     2881 tgaagaccgt ggacggacat cccaccgtga tccagacccc gacccgcgcg tacgaggaca
     2941 tggtcaggaa aaagggagtc gtgacctacg gggaccggaa cctgccgcag gacgggcgcg
     3001 tgacgatccc gtcccggggc gaggtcctgg acatccgcct ggtgctgcgg cgcggcggcg
     3061 cccggcgcgc cggcatcgtg gtacgggaaa cgccggacgg caggaccggc acgtcggtca
     3121 gctacgattt cgtggacggt accctgaccg tggatcgcgg ccgttccggc ctggtcggct
     3181 tctcgccccg cttcagcacc cggcatatcg cgtacctggc cgcgccgggc ggcgaagtcg
     3241 cgcttcatct ggtcgtcgat cgcgcttcgg tcgaactgtt cgccaatgat ggggtgctgc
     3301 gcatgaccga cctgatcttc cccccggcgg gaagcgaccg gatctcgctg tttgccgaag
     3361 ggggcggcgc caccattcac ggattgcggg tggcggtgct ggcccgatga
//
