LOCUS       NC_004368              10661 bp    DNA     linear   CON 13-FEB-2020
DEFINITION  Streptococcus agalactiae NEM316, complete sequence.
ACCESSION   NC_004368
VERSION     NC_004368.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3138330
            Assembly: GCF_000196055.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus agalactiae NEM316
  ORGANISM  Streptococcus agalactiae NEM316
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Glaser,P., Rusniok,C., Buchrieser,C., Chevalier,F., Frangeul,L.,
            Msadek,T., Zouine,M., Couve,E., Lalioui,L., Poyart,C., Trieu-Cuot,P.
            and Kunst,F.
  TITLE     Genome sequence of Streptococcus agalactiae, a pathogen causing
            invasive neonatal disease
  JOURNAL   Mol. Microbiol. 45 (6), 1499-1513 (2002)
   PUBMED   12354221
REFERENCE   2
  AUTHORS   Glaser,P., Rusniok,C. and Frangeul,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2002) Glaser P., Institut Pasteur, Genomique des
            Microorganismes Pathogenes, 25 rue du Docteur Roux, 75724 Paris
            Cedex 15, FRANCE. E-mail: pglaser@pasteur.fr Phone: +33 (0)1 45 68
            89 96, Fax: +33 (0)1 45 68 87 86
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2020 22:08:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 2,217
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,083
            CDSs (with protein)               :: 2,083
            Genes (RNA)                       :: 104
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 19 of 30
            Pseudo Genes (incomplete)         :: 7 of 30
            Pseudo Genes (internal stop)      :: 7 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            AL732656.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Streptococcus agalactiae NEM316"
                     /mol_type="genomic DNA"
                     /strain="NEM316"
                     /serovar="III"
                     /db_xref="taxon:211110"
     gene            complement(1..816)
                     /locus_tag="GBS_RS09830"
                     /old_locus_tag="gbs1886"
     CDS             complement(1..816)
                     /locus_tag="GBS_RS09830"
                     /old_locus_tag="gbs1886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR74|4.A.6.1.14"
                     /protein_id="WP_000147951.1"
                     /translation="MTGSKKLAKSDYTKTVLRAFYLQNGFNYSNYQGLGYANVIYPALK
                     KYYGDDKKALAGALEENVEFYNTNPHFLPFVTSLHLAMLDNERPEEEIRGIKMALMGPL
                     AGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYRLGT
                     SFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTTQKIVTVQGM
                     LDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(803..1669)
                     /locus_tag="GBS_RS09835"
                     /old_locus_tag="gbs1887"
     CDS             complement(803..1669)
                     /locus_tag="GBS_RS09835"
                     /old_locus_tag="gbs1887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR75|4.A.6.1.14"
                     /protein_id="WP_000355267.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGDI
                     QTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALALS
                     TPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLGVLG
                     ALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYVLAVY
                     FGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKETKTEG
                     DNQDDWI"
     gene            complement(1705..2199)
                     /locus_tag="GBS_RS09840"
                     /old_locus_tag="gbs1888"
     CDS             complement(1705..2199)
                     /locus_tag="GBS_RS09840"
                     /old_locus_tag="gbs1888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR76|4.A.6.1.14"
                     /protein_id="WP_000718802.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDHL
                     QQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIKEI
                     NIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNILDY
                     I"
     gene            complement(2254..3450)
                     /locus_tag="GBS_RS09845"
                     /old_locus_tag="gbs1889"
     CDS             complement(2254..3450)
                     /locus_tag="GBS_RS09845"
                     /old_locus_tag="gbs1889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH88"
                     /protein_id="WP_000975716.1"
                     /translation="MMKIKPVKVESIENPKRFLNSRLLTKIEVEEAIEKALKQLYINID
                     YFGEEYPTPATFNNIYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFLNR
                     INNRIALDHHDLGFLYTPSCTAEYRINGDVKALEATIKAADKLMERYQEKGGFIQAWGE
                     LGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAFHTFY
                     FDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMTNYFLN
                     RLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKYLPEVDPDKETYKYAMHTML
                     RSLIEQYSNNELIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDWELYW"
     gene            complement(3453..3887)
                     /locus_tag="GBS_RS09850"
                     /old_locus_tag="gbs1890"
     CDS             complement(3453..3887)
                     /locus_tag="GBS_RS09850"
                     /old_locus_tag="gbs1890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR78|4.A.6.1.14"
                     /protein_id="WP_000590444.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDVR
                     VALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLSRT
                     MFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            4165..4977
                     /gene="dhuD"
                     /locus_tag="GBS_RS09855"
                     /old_locus_tag="gbs1891"
     CDS             4165..4977
                     /gene="dhuD"
                     /locus_tag="GBS_RS09855"
                     /old_locus_tag="gbs1891"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="WP_000139169.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFND
                     IKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVDKISQDVGVIDILVNNAGIIKR
                     TPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETVAAY
                     AAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQFIIA
                     KTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            4994..5632
                     /gene="dhuI"
                     /locus_tag="GBS_RS09860"
                     /old_locus_tag="gbs1892"
     CDS             4994..5632
                     /gene="dhuI"
                     /locus_tag="GBS_RS09860"
                     /old_locus_tag="gbs1892"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="WP_000684791.1"
                     /translation="MKIALINENSQASKNTIIYKELKAVSDEKGFEVFNYGMYGKEEES
                     QLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADPVDAYLFSQ
                     VNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSEIKQ
                     ITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            5658..6665
                     /locus_tag="GBS_RS09865"
                     /old_locus_tag="gbs1893"
     CDS             5658..6665
                     /locus_tag="GBS_RS09865"
                     /old_locus_tag="gbs1893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|PfkB"
                     /protein_id="WP_000034943.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNTARALQG
                     FGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLEDSFACRKGEVVY
                     DRDHSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREITIS
                     LDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDVKERM
                     ISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGSGDAFV
                     AGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDIIR"
     gene            6677..7315
                     /locus_tag="GBS_RS09870"
                     /old_locus_tag="gbs1894"
     CDS             6677..7315
                     /locus_tag="GBS_RS09870"
                     /old_locus_tag="gbs1894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="WP_000597408.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEIA
                     YTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDSETARMAILAGAKFIVSPAFNLQTAKL
                     CNRYAIPYLPGCMTLSEVTTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMVTGG
                     VNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
     gene            8515..9876
                     /locus_tag="GBS_RS09875"
     CDS             8515..9876
                     /locus_tag="GBS_RS09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000440333.1"
                     /translation="MERLKSSGLSMGKPVDYMGVNGIPIYTKTLSIDNKFAFENNSKDS
                     SYSSNINISEDKIKENDQKILDLIVKSGANNQNLTDEEKVIAFTKYIGEITNYDNEAYR
                     ARNVDTEYYRASDLFSVTERKLAMCVGYSVTAARAFNIMGIPSYVVSGKSPQGISHAAV
                     RAYYNRSWHIIDITASTYWKNGNYKTTYSDFIKEYCIDGYDVYDPAKTNNRFKVKYMES
                     NEAFENWIHNNGSKSMLFINESAALKDKKPKDDFVPVTEKEKNELIDKYKKLLSQIPEN
                     TQNPGEKNIRDYLKNEYEEILKKDNLFEHEHAEFKESLNLNESFYLQLKKEEMKPSDNL
                     KKEEKPRENSVKERETPAENNDFVSVTEKNNLIDKYKELLSKIPENTQNPGEKNIRNYL
                     EKEYEELLQKDKLFKHEYTEFTKSLNLNETFYSQLKEGEMKLSENPEKGETNTN"
     gene            complement(10047..10661)
                     /locus_tag="GBS_RS09880"
                     /old_locus_tag="gbs1896"
     CDS             complement(10047..10661)
                     /locus_tag="GBS_RS09880"
                     /old_locus_tag="gbs1896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|Nitroreductase"
                     /protein_id="WP_000008878.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNSQ
                     TSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLFFE
                     DQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVIDASV
                     QAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
ORIGIN
        1 ttaagctaaa atacctaacc agctaccaag gataccaata ataactgtaa ggataactaa
       61 cttataagtt gtccattttt tatttttaat aaggtaaaac ataaggaaag tgtagagtgc
      121 tggaagtaga gctggtgcaa ttttatcaag cataccttgg acagtaacaa tcttttgcgt
      181 tgtactagtt actttttctg gagcaaaagt gtaagggatt gtaagcttaa cttgtgttgc
      241 agctaaactt gagatgaccg ttacaccaac gatattagct gcccgtgaga taactgacat
      301 ttgttcactc aatttatcaa taaaactggt cccaagacga taaccataca tgccagtaac
      361 taatttaatt cccgttaaaa tagtattcat agccacaaag aacagaatcg gtcccatcac
      421 caaaccatct gtcgctaaag aggctgcaat cgttgagaac aagggtgcta agcaaaattg
      481 tgataaagaa tcaccgatac ctgcaagagg ccccataagc gccatcttaa taccgcgaat
      541 ttcttcttct ggccgctcat tatcaagcat tgctaaatgt aagctagtta caaaaggtaa
      601 gaaatgaggg tttgtattgt aaaactccac attttcttct agagctcccg ctaaagcttt
      661 tttgtcatct ccataatatt ttttgagagc tggatatatg acattagcat aaccaagacc
      721 ttgatagtta ctgtaattaa agccattttg taaataaaag gcacgtaaaa ctgttttagt
      781 gtaatcactt tttgctaatt ttttagatcc agtcatcttg gttgtctcct tctgtcttag
      841 tttctttagt aggtgcagct ggttttctga aaatatcaag caaagcaaat attgttgcaa
      901 ttatagctac cccgatagtt ggaaccccaa gcacactgtt agtagccact gatgttaaaa
      961 ctccgtctcc atttgctgtt ggagttagaa ctggcaaacc aaaataaaca gctagaacat
     1021 atccaagtag gatataagga atgagttctt ttttagccat tacagagaga atcatcgcaa
     1081 aaccaattgc aggaagcatt ttcccagcta aggttagacc attaagcaaa acagggggaa
     1141 tgagagcaaa taaatctgtc aaagtttgcg ttgaaagagc acccagtact cctaaaccaa
     1201 atcccaatcc agcgaatgcc caaattgtac catttgctgc aattttaaat cctcggaaat
     1261 tacctgcttg caaagctttt ttagctgttt caggcgcccc tgcaaaagca gtataagttg
     1321 ctgtttgaag aaattgaata ccaactgcaa ttggggttga taatgcaaga gcagcttccg
     1381 gtgtgatttt tcctttagtt ccagctgttg taatagccat caaagttcca aaaatcccag
     1441 gaccgattgg gtttggagga actgtccctc ccgctcccac accaaatccc atgtaagcca
     1501 gttcagatat tgctccaact gccaaagctg tttgaatatc tcctaaaata acaccaacgc
     1561 ctagagagag aacgatacaa cgatttgtat agaggccaag caacatgcca ctaaaacaga
     1621 aagccgtcca cagtccaatt aagacagcct gaagaatact aatatccata tttttctcct
     1681 ttatatgata gtaagtttgt ccaactaaat ataatctaaa atattaactt ccacagcacc
     1741 atcgttacct gttggagttg ttttagtatt aaatgcaatg tgatattctt ggtttaattt
     1801 tctaattatc tctttatcct tcatccctaa gaagatagag cgacttacct gctctttacc
     1861 ttctccatta tgaatatttc caatattaat ctctttaatt gggacgcccc ctgctacaag
     1921 tctatagaca tctttcaaat ctttaacaat tataaaaatc gtctgtgctg ggttggcctt
     1981 gtgaataata tcaataactt tttgaatatc aaaaaatcgc aatgctattg attctggcac
     2041 caccgtcttc attaacgttt gttgaagatg atctttggag acatcatcat ttgccacaat
     2101 aacagtatta caacttaaaa atttcaccca aagttgtcct tgtccatgaa taagacgctc
     2161 atcaacacgt gtcattacaa tatttggtcc tgctgccatt tctgacctcc tcgttttcta
     2221 ttatttattg tttattttgt tagtaaaata ctcttaccaa taaagttccc agtctttata
     2281 gaatcttatt aaggcttcta agtaataata atctccccaa atattacctt catctactcc
     2341 tttacctgaa tgccacgaat acacaccgtg caatagaaga ggacgtcctg ctataagttc
     2401 attattacta tactgttcaa tcagactacg aagcattgta tgcatagcat atttgtatgt
     2461 ctctttatca ggatctactt ctggtaaata tttaagcatc tcatgaattc cacacacagc
     2521 cgttgctgtt gcggatgtat ctctaggctg gcccgagcca tccgtaaaaa taaggtccca
     2581 ataggatacc ttgtcttcag gtaaacgatt tagaaaatag tttgtcatac ctttaaaaag
     2641 gataatctgc tgataatctt tcattttccg gtaactaagc ggaataccgt agatgcccca
     2701 tgcttgccct cttgcccaag atgactcatc actataaccc tgtcgtgtga caccttttaa
     2761 cggttctcca gtttctgggt cgaaataaaa agtatgaaaa gcagaagaat catcacgcac
     2821 cacattgtta gctgaagcgt agaagtgatt caccgcaact tgtctatact tttcatcacc
     2881 tgtctgttca taagcaaaaa ataagagttg gatattaagt aagcaatcga taattaagcg
     2941 atagtgttcc ttatacccga gttctcccca agcctgaata aatccacctt tttcttgata
     3001 gcgctccatc aatttatctg cagcttttat agtggcttct aaagctttaa catcaccatt
     3061 gatacgatat tctgctgtac aagatggtgt gtaaagaaat cctaagtcgt ggtgatctaa
     3121 tgctatacga ttattaatac gatttagaaa tgacaataca tttttgtggg ctatgttttt
     3181 taacttttta tcctgattat actcataagc taaccacaag caccctgtcc aaaaaccatt
     3241 tgtccattct gtgttatcca taactttata aatattattg aatgttgcag gcgttggata
     3301 ctcttcacca aagtaatcaa tattaatata aagttgcttc aaggcttttt caatcgcttc
     3361 ctcaacttca atcttagtta ataatctact gtttaaaaaa cgctttggat tttcaattga
     3421 ctcaaccttg accggtttta ttttcatcat tattaaatgc ctccctcaaa agttgcttct
     3481 gcagtttgtg tagcaaggca agtagaaaaa tcaacaattc cttcatgaga tgatgttata
     3541 actttatcaa caagatcatc tacatgttca aacatcgttc tagataaaac agcttccatt
     3601 aacattgaaa ggttcaagcc cgataaaact tttatctttt tatctgtata ttctactgat
     3661 aaggcggaag aaacattgaa aggagtccca cctagcaaat cagtcaatac taaaatttct
     3721 ttgaaatcga taacctctct ttgcaaagca actctaacat cattagaaga catgcctgca
     3781 ataaagttaa taccaacaac atattcctga tgcccagcaa taagttctaa agagcttaaa
     3841 ataccatctg gaaagttgcc gtgtgctaca ataataattt ttatcatatt ttctcctctt
     3901 gagtttaccg gtttactttt ataatcagat tatagcgctt tcatttttag gtgtcaacat
     3961 cttttttata ttttttgagc agtttacttg attttttata atgatgtctt ttttatcttt
     4021 aagaattata acctagataa atctttgtta tatatgttgc caagttttat agaagaagaa
     4081 ctctatagtt gacaaaaccg gtttactttg ttactattca tgtaaacagg tttacttcct
     4141 gaattttttt acggaggtca tattatgact gaacaattct taaaagacaa cttttcacta
     4201 gaaggtaaag tcgccttaat aacaggcgct tcttacggca ttggtttttc aatagcaact
     4261 gcttttgcta gagctggagc aactattgtc ttcaatgata ttaagcaaga actcgttgac
     4321 aaaggcatct ccgcctataa aaaacttggc attaaggccc atggctatgt ttgtgatgtt
     4381 acagatgaag atggaatcaa cgaaatggtg gacaaaattt ctcaagatgt tggggtaatt
     4441 gatattctag ttaacaacgc aggtattata aaacgcaccc ctatgctaga gatgagcgct
     4501 gcagattttc gacaagttat tgatattgat ttgaatgctc cctttatcgt atcaaaagcg
     4561 gtattacctg gaatgatcca aaaaggacat ggtaaaatca tcaatatttg ctccatgatg
     4621 agtgagcttg gacgcgaaac agttgctgcc tatgctgctg ccaaaggggg acttaaaatg
     4681 ttaaccaaga acatcgcttc tgaatacggt tctgctaaca tccaatgtaa cggtatcggg
     4741 cccggctata ttgccacacc acaaacagct ccccttcgtg aacgtcaaga tgatggttca
     4801 cgtcatccat tcgaccaatt tatcatcgct aaaacacctg ccgctcgctg gggtgaggca
     4861 gaagatttag gtgcaccagc tatcttcctt gctagtgatg catcaaactt tatcaatggt
     4921 cacatactct atgtagatgg cggtatttta gcttacatcg gtaaacaacc tcaataataa
     4981 aggagtaata taaatgaaaa ttgcattaat caacgaaaat agccaagcaa gtaaaaatac
     5041 tattatctac aaagaattga aagcggtttc tgatgaaaaa ggatttgaag ttttcaacta
     5101 cggaatgtat ggcaaagaag aagaaagcca gctaacttat gtacaaaatg gtttactgac
     5161 tgctatttta cttaatagcg gtgctgcaga ttttgttatt acaggctgtg ggacaggaat
     5221 tggagcgatg cttgcttgta atagttttcc tggcgtagtc tgtggattcg ctgctgatcc
     5281 tgtcgacgct tacttattct ctcaagtaaa cggaggaaat gctctttccc ttccttttgc
     5341 taaaggattt ggttggggag ctgaattgaa tctgaggtat ttatttgaac gtcttttcga
     5401 agatgaaaaa ggagggggat accctaaaga gagggctgta cctgagcaac gcaatgcacg
     5461 tatccttagc gaaataaaac agattactta cagagattta ctctctgttt taaaagaaat
     5521 tgatcaagat ttcttaaagg aaactatttc tggggagcat ttccaagaat atttcttcgc
     5581 taattgccaa aatcaaaata tagcagacta cttgaaatct gtcttagact aaagcgtctg
     5641 ttttagaaag gacttttatg tctaagattc tttttttcgg tgaacctctc attcgcatca
     5701 ctccaaaaga aaatgactat tttgcagata gtatctcaac taaactattc tatggtggct
     5761 ctgaagttaa taccgcaaga gctctacaag gctttgggca agatactaaa ctcctctcag
     5821 ccttacccaa taatcccata ggtaatagtt ttcttcaatt tttaaaagca cagggtattg
     5881 atactcattc tattcagtgg gttggagaac gtgttgggtt atatttttta gaagattcct
     5941 ttgcttgcag aaaaggtgag gtggtctacg accgcgatca tagtagttta catgacttta
     6001 ggataaatca aattgatttc gatcaattat ttgaaggagt ttctctcttt cattttagtg
     6061 gtattacact atcattggat gagtctatcc aagagataac actgcttctt ttaaaagaag
     6121 ctaaaaaaag agagataaca atctccttag atctcaattt tcgcagtaaa ctaatttctc
     6181 ctaaaaatgc caaaatcctt ttttctcagt tcgctacctt tgcggatatt tgttttggta
     6241 ttgaacctct tatggttgac tcacaggaca ctaccttctt caatcgcgat gaagctacaa
     6301 tagaggacgt caaagaacga atgatttcgc ttataaatca ctttgacttt caagtcattt
     6361 tccatactaa acgtctacag gatgaatggg gacgaaacca ctaccaagct tacatagcaa
     6421 atcgtaaaca agagtttgtc acttcaaaag aaatcaccac agctgtaaac cagagaattg
     6481 gaagcggaga tgcttttgtg gcgggagcgc tatatcaact acttcagcac tcagacagca
     6541 agacagtcat tgatttcgct gtcgctagtg catctttaaa atgtgctttg gaaggggaca
     6601 atatgtttga gaccgttacc gcagttaaca aagtactaaa tcaatcgaaa gacattattc
     6661 gttagaaggg agaattatga taaaatcaaa gattttgttg caattaatgc aaggaaaaat
     6721 agttgctgtc attcgtggaa atagccaaga agaagctttt caagctgctc aagcatgcat
     6781 aaaaggtggt atttctgcta tcgaaatcgc ctatactaat agcaaagcta gccaagtcat
     6841 tgaacaacta gtcacacaat atactaacca agaacaagtt gtcgttgggg caggaactgt
     6901 tctagactca gaaactgccc gaatggctat tttagcagga gcaaagttta ttgtatctcc
     6961 cgcatttaat ctacagactg ctaaactatg taatcgttat gctattcctt atttaccagg
     7021 ttgcatgacc ttatcagagg taacaacagc acttgaagcc ggttgcgaaa taattaaaat
     7081 attcccagga ggtactctag gaacatcttt catctcatca ctaaaggcac ctttacctca
     7141 agtacaaatt atggtgactg gtggggtaaa tctcacaaat gccaaagact ggtttctatc
     7201 tggagtaaca gccattggta ttggtggaga gttcaataaa ctagcagctt tgggggaatt
     7261 tgataaaatc acagaaatgg ctaaacaata caaaatacag ctaaatctga attaacacaa
     7321 taaaaagaca aggcaccccc acacctcaat catgagacag aagaaaagaa cttttataaa
     7381 gccatttttc tgtagtaccg tttatcttaa tcagactcat tatctcgatt ttcttatcag
     7441 accgagataa tttttagcta atcttcctgt agacataacc gttctacttc cgacaactga
     7501 tccatgtctt caccaaagat atactacttg cccgcagatt gatataagta gtgaagctca
     7561 tcattctcat atcatttcag caaataatag atttgatatc atttttatat ccaaaaactt
     7621 agcaatgacc tatcaggctt acccgctttt cctatctcaa cacagactaa cttagctcct
     7681 atcaaataca ttttttaaac ataattaaaa acatttctga ttatagttta ttagatttca
     7741 acagaagtat tttttctgcg tctcagcttt tgagtgtacc gttattttga agttttgata
     7801 gttgagtgat atcattaata gtcgtttttt agacacgcta aacctccttt ctaatttgac
     7861 tgattttatt ttttataacg actcgtttta ctgttatatc tctttataat cacctattta
     7921 aatagacgca tttaaaatca aaattaaccc aagattttta ttgaaaaaca ttttttaata
     7981 tgttattatt tatttattaa taaattagga gatttttaaa aaatgaagaa aaaaagatta
     8041 aaattaactt gtgtcttatc atttgctgta gcttcaacat tattcaacct atctgattta
     8101 aatatatcat atgctaatca aaataatatt caagaaacta atctagttga aaaaaacagt
     8161 gaagataagt ttattcaaga gttaaatagg tataaaacag agataccaaa tttcaaaggt
     8221 tttaatgttt ggattttagg tgataaaggc tattacaaaa accttatcaa tttagaagaa
     8281 attaaaaata tccaagctac attaaaaaaa gaaagaaatg aagaatatgt ctttgtcaaa
     8341 ttaaatggga aaatcgcaca tgatacaacc gtattcttga tgaataaaaa acataaatta
     8401 cttaaaaata tagaggaatt taagacgata acccaaaaaa ggctaactga gagaggaaag
     8461 ttcccatatg ataccgttca ttctacgttt gaaattaagg atgaaaactt tatcatggaa
     8521 agattgaaat catcagggtt atctatgggt aaaccagttg attatatggg cgtaaatggt
     8581 atccctattt acactaaaac attatctata gataataagt ttgcctttga aaataattca
     8641 aaagattctt catattcttc aaacattaat attagtgaag ataaaataaa agaaaatgac
     8701 caaaaaattc tagatttaat agttaaatct ggagctaata atcaaaactt aacagatgaa
     8761 gaaaaagtaa ttgctttcac taaatatata ggtgaaataa ctaattatga caatgaagct
     8821 tacagagcta gaaatgtcga cacagaatat tacagagctt cagatttatt ttctgtaaca
     8881 gaaagaaaac ttgctatgtg cgttggctat agtgtaacag ccgctagggc atttaatatt
     8941 atggggatac ctagttatgt cgtttctggt aaaagtcctc aaggaatatc tcatgcagct
     9001 gtgagagcgt attataatag aagttggcat atcatagata tcacagccag tacatattgg
     9061 aaaaatggta attacaaaac aacgtattcc gattttatta aagaatactg tatagatggt
     9121 tatgacgttt atgatcccgc aaaaacaaat aatagattta aagttaagta tatggaatca
     9181 aatgaagcgt ttgaaaattg gatacataat aatggaagca aatctatgtt atttattaat
     9241 gaatctgcag ctctaaaaga taaaaaacca aaagatgatt ttgtaccagt aacagaaaaa
     9301 gaaaaaaacg agctgataga taagtataaa aaattattgt cacagattcc tgaaaacaca
     9361 caaaatccag gagaaaaaaa cataagagat tatttgaaaa atgaatatga agaaatatta
     9421 aagaaagata atttatttga gcatgaacat gcagaattta aagaaagtct aaatttaaat
     9481 gaatcattct acctacaatt aaagaaagaa gaaatgaaac cgagtgataa tctcaaaaaa
     9541 gaagaaaaac caagagaaaa ttcagtaaaa gagagggaga ctccagctga aaacaatgat
     9601 tttgtttcag taacagaaaa aaacaacttg attgataaat ataaagaatt attatcaaaa
     9661 atcccagaga atacacaaaa tcctggggaa aagaatatac gaaattatct agagaaagaa
     9721 tatgaagaac tactacaaaa agataaatta ttcaagcatg agtacacaga gtttacaaaa
     9781 agtctaaatt tgaatgaaac attctattca caattaaaag aaggagaaat gaaactaagt
     9841 gaaaatcccg aaaaaggaga aacgaacacg aattaacctt tcaaatatct acatcaaata
     9901 acagaagtct ctgtatcaag cgaattgtag cttttttgaa tatactaaaa acactcctac
     9961 ctctatttta ctagaagtag gagtgttttt tcattttagt tcatagatgc agtgtcattg
    10021 ctatccttgc ctttttttgt acagtattat ccgatgactt taaagcggtc atcatcattc
    10081 atgaattctt tttctcctgt ttcagcttcg attgaaccaa aatttagttg tccacgaagt
    10141 ttccaactag ctggaacacc ataaacagct tgtactgatg catcaataac tggattgtaa
    10201 tgttgtaaat taccaccaag tccaagttct gcagaaaggg ctgtccaagt atttactgag
    10261 gcaatacctg ttgattgctc agaccaaact ggaaagttat ccgcataaag aacgaattgt
    10321 tcttgaagag atttgacaac atcttgatct tcaaagaaga gtactgttcc ctttgaagct
    10381 ccaaaactag ctaatttttc cttagttcca gcaatagctg tctctggagc accttgtact
    10441 ttcattgttt ctaccaaatc attagcaacc aattcatccc agaacttagt aacttcatca
    10501 ttcaataaaa tgacaacacg ggaagtttga ctattaaaag cagatgggga ttggcgaact
    10561 gcctctttaa taatctctac tattttctca tcagaaactt ctgtattgcg accaagagcg
    10621 taaatagagc gacggttttt taattcttcc aaaaatgaca t
//
