LOCUS       MG739441               15991 bp    DNA     linear   BCT 31-JAN-2018
DEFINITION  Escherichia coli strain K92 K-antigen gene cluster, complete
            sequence.
ACCESSION   MG739441
VERSION     MG739441.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   Yang,S., Xi,D., Jing,F., Kong,D., Wu,J., Feng,L., Cao,B. and Wang,L.
  TITLE     Genetic diversity of K-antigen gene clusters of E. coli and their
            molecular typing using a suspension array
  JOURNAL   Can. J. Microbiol. (2018) In press
   PUBMED   29357266
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15991)
  AUTHORS   Yang,S., Xi,D., Jing,F., Kong,D., Wu,J., Feng,L., Cao,B. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2017) TEDA, Nankai University, Hongda Street 23,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SOAPdenovo v. SOAPdenovo2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="K92"
                     /isolation_source="G5779"
                     /db_xref="taxon:562"
     misc_feature    1..15991
                     /note="K-antigen gene cluster"
     gene            1..984
                     /gene="kpsF"
     CDS             1..984
                     /gene="kpsF"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SIS,STP|CBS,STP|CBS"
                     /protein_id="AUT31362.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQY
                     QRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMITPY
                     DLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANETCPN
                     NLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVP
                     AVQLDASFKTVIQRITNGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQM
                     MTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1056..2204
                     /gene="kpsE"
     CDS             1056..2204
                     /gene="kpsE"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P42501|8.A.4.1.1"
                     /protein_id="AUT31363.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYLA
                     IFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLAAL
                     DKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGFSPE
                     FALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQDNNNV
                     LDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQAQIDEE
                     KSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKVLSVISS
                     PQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2228..3904
                     /gene="kpsD"
     CDS             2228..3904
                     /gene="kpsD"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q03961|1.B.18.1.2"
                     /protein_id="AUT31364.1"
                     /translation="MKLLKSILLIAACQAAQASAAIDINADPNLTGAAPLTGILSGQKS
                     DTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVRLW
                     GAFTFDGALQIDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYASLLQ
                     AQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVRSNVN
                     LYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEALSWAR
                     PKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVRVEGAHS
                     GEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQKLEEASLS
                     AQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGDVINIPEKT
                     SLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGAAVNAEDVDS
                     LKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            3914..4654
                     /gene="kpsU"
     CDS             3914..4654
                     /gene="kpsU"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="AUT31365.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGVA
                     EVWVATDDPRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHTVEADIYINLQGDEPMIR
                     PRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTRQDALYFSRSPIPYPR
                     NAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEVAATG
                     PGVDTPACLEKVCALMAQKLAENA"
     gene            4651..6678
                     /gene="kpsC"
     CDS             4651..6678
                     /gene="kpsC"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P42217|9.A.41.1.2"
                     /protein_id="AUT31366.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGHR
                     PSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSALEK
                     LVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFNDMSV
                     TYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLIAENV
                     SPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALLSARRG
                     SATLEELFAAAYLRYCRYIDPQTGEVSDLFTVLQWLQLQRNHQQQRNGYLWAPGLTLWK
                     SAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRMEDGFLRS
                     SGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQKMRAEKLRQRLVESKL
                     SKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTVRERNPHAYI
                     IYKPHPDVLVGNRKGDIPAEQIAELADYQALDADIIQCIQRADEVHTMTSLSGFEALLH
                     GKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTLADLIYQALIVYPTYIHPTRLQPITVE
                     EAAEYLIKTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            6713..7918
                     /gene="kpsS"
     CDS             6713..7918
                     /gene="kpsS"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P42218|9.A.41.1.3"
                     /protein_id="AUT31367.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVVF
                     NGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAKSK
                     GIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLKPSTMKRI
                     GHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPRLMNE
                     LDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHPMDRGH
                     RLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHNKPLKVM
                     GNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLMKTQVNAVYYGGNTKNRQHN
                     TY"
     gene            complement(8061..9290)
                     /gene="neuS"
     CDS             complement(8061..9290)
                     /gene="neuS"
                     /codon_start=1
                     /transl_table=11
                     /product="GT38"
                     /protein_id="AUT31368.1"
                     /translation="MIFDASLKKLRKLFVNPIGFFRDSWFFNSKNKAEELLSPLKIKSK
                     NIFIVAHLGQLKKAELFIQKFSRRSNFLIVLATKKNTEMPRLILEQMNKKLFSSYKLLF
                     IPTEPNTFSLKKVIWFYNVYKYIVLNSKAKDAYFMSYAQHYAIFIWLFKKNNIRCSLIE
                     EGTGTYKTEKKKPLVNINFYSWIINSIILFHYPDLKFENVYGTFPNLLKEKFDAKKIFE
                     FKTIPLVKSSTRMDNLIHKYRITRDDIIYVSQRYWIDNELYAHLLISTLMRIDKSDNAR
                     VFIKPHPKETKKYINAIQGAINKAKRRDIIIIVEKDFLIESIIKKCKIKHLIGLASSSL
                     VYAPLVYKECKTYSIAPIIIKLCNNEKSQKGINTLRLHFDILKNFDNVKILSDDITSPS
                     LHDKRIFLGE"
     gene            complement(9280..10455)
                     /gene="neuE"
     CDS             complement(9280..10455)
                     /gene="neuE"
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid biosynthesis protein"
                     /protein_id="AUT31369.1"
                     /translation="MTSRNVPPVMTRKKVLCFVFRYDSHFLALKNIFEQIDVDSYDLFF
                     CCLDNSLQEFVKKNLDEKIVVFYPDDFVYFFTFINIEFIFCSTGGKDLHEIVNTLRTKN
                     TIIISCFPGIVLTSQIEAFISKSNSHYLLINSPKDIKTYKKICKIIGVPFNGILFGPPW
                     IKNVNINAKSENSCLIVDQVNEPLTPIKRIEYARFLIRVIQKHPHMNFIFKTRNPLMSP
                     DSIVFDIKEYIERFDLKNITFSDDNIDSLISKVEYCITISSSVAIYCLANKIKVYLING
                     FNHTCNGQCYFSRSGLIVDYNKLNFKHIPRIKKKWMEENLYYSRDIQNKILNDILKMPP
                     NVNVRTFGIKRSTLIILFLIFLNFFFSLGPKKIKTLKKIHKVLLRYKKDDI"
     gene            complement(10421..11596)
                     /gene="neuC"
     CDS             complement(10421..11596)
                     /gene="neuC"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="AUT31370.1"
                     /translation="MKKILYVTGSRAEYGIVRRLLTMLRETPEIQLDLAVTGMHCDNAY
                     GNTIHIIEQDNFNIIKVVDININTTSHTHILHSMSVCLNSFGDFFSNNTYDAVMVLGDR
                     YEIFSVAIAASMHNIPLIHIHGGEKTLANYDEFIRHSITKMSKLHLTSTEEYKKRVIQL
                     GEKPSSVFNIGSLGAENALSLHLPNKQELELKYGSLLKRYFVVVFHPETLSTQSVNDQI
                     DELLSAIYFFKNTHDFIFIGSNADTGSDIIQRKVKYFCKEYKFRYLISIRSEDYLAMIK
                     YSCGLIGNSSSGLIEVPSLKVATINIGDRQKGRVRGASVIDVPVEKNAIVRGINISQDE
                     KFISVVQSSSNPYFKENALINAVRIIKDFIKSKNKDYKDFYDIPECTTSYD"
     gene            complement(11593..12849)
                     /gene="neuA"
     CDS             complement(11593..12849)
                     /gene="neuA"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="AUT31371.1"
                     /translation="MRTKIIAIIPARSGSKGLRNKNALMLIDKPLLAYTIEAALQSEMF
                     EKVIVTTDSEQYGAIAESYGADFLLRPEELATDKASSFEFIKHALSIYTDYENFALLQP
                     TSPFRDSTHIIEAVKLYQTLEKYQCVVSVTRSNKPSQIIRPLDDYSTLSFFDLDYSKYN
                     RNSIVEYHPNGAIFIANKQHYLHTKHFFGRYSLAYIMDKESSLDIDDRMDFELAITIQQ
                     KKNRQKILYQNIHNRINEKRNEFDSVSDITLIGHSLFDYWDVKKINDIEVNNLGIAGIN
                     SKEYYEYIIEKELIVNFGEFVFIFFGTNDIVVSDWKKEDTLWYLKKTCQYIKKKNAASK
                     IYLLSVPPVFGRIDRDNRIINDLNSYLRENVDFAKFISLDHVLKDSYGNLNKMYTYDGL
                     HFNSNGYTVLENEIAEIVK"
     gene            complement(12849..13889)
                     /gene="neuB"
     CDS             complement(12849..13889)
                     /gene="neuB"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl neuramic acid synthetase NeuB"
                     /protein_id="AUT31372.1"
                     /translation="MSNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKADK
                     LISAIAPKAEYQIKNTGELESQLEMTKKLEMKYDDYLHLMEYAVSLNLDVFSTPFDEDS
                     IDFLASLKQKIWKIPSGELLNLPYLEKIAKLPILDKKIIISTGMATIDEIKQSVSIFIN
                     NKVPVDNITILHCNTEYPTPFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPY
                     GITFIEKHFTLDKSMSGPDHLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKI
                     VARKSIIAKTEIKKGEVFSEKNITTKRPGNGISPMEWYNLLGKIAEQDFIPDELIIHSE
                     FKNQGE"
     gene            complement(13886..14509)
                     /gene="neuD"
     CDS             complement(13886..14509)
                     /gene="neuD"
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid synthase NeuD"
                     /protein_id="AUT31373.1"
                     /translation="MSKKLIIFGAGGFSKSIIDSLNHKHYELIGFIDKYKSGYHQSYPI
                     LGNDIADIENKDNYYYFIGIGKPSTRKHYLNIIRKHNLRLINIIDKTAILSPNIILGDG
                     IFIGKMCILNRDTRIHDAVVINTRSLIEHGNEIGCCSNISTNVVLNGDVSVGEETFVGS
                     CTVVNGQLKLGSKSIIGSGSVVIRNIPSNVVVAGTPTRLIRGNE"
     gene            complement(14559..15218)
                     /gene="kpsT"
     CDS             complement(14559..15218)
                     /gene="kpsT"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P24586|3.A.1.101.1"
                     /protein_id="AUT31374.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGKS
                     TLLRIIGGIDRPDSGNIITEHKISWPVGLAGGFQGSLTGRENVKFVARLYAKRDELNER
                     VDFVKEFSELGKYFDMPIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITSVGDAKFKKKC
                     SDIFDKIREKSHLIMVSHSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKDL"
     gene            complement(15215..15991)
                     /gene="kpsM"
     CDS             complement(15215..15991)
                     /gene="kpsM"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P24584|3.A.1.101.1"
                     /protein_id="AUT31375.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLLI
                     LLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISKRSIGAIEANQGLFNYRPVKPID
                     TIIARALLETLIYVAVYILLMLIVWMAGEYFEITNFLQLVLTWSLLIILSCGVGLIFMV
                     VGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREAVMPG
                     YISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
ORIGIN
        1 atgtctgaaa gacatttacc tgatgaccag agcagtacta tcgatccata tctaattacc
       61 tctgttcgcc agactctggc agaacaaggc gcagcattac aaaacttgtc taaacaactg
      121 gattccgggc agtaccagcg tgtccttaat ttgataatga actgtaaagg gcacgttatt
      181 ctttcgggaa tgggtaaatc agggcatgtc ggtcgtaaaa tgtcagcgac gctggcctct
      241 acgggtacgc ctagtttctt tattcatcct gcagaagctt tccatggcga tctgggcatg
      301 attacgcctt acgatcttct gatccttatt tctgccagcg gtgaaacgga tgaaatcctc
      361 aagctagttc cttcactgaa aaatttcggc aaccgaatta tcgccattac caataatgga
      421 aattccacgc tggcgaaaaa tgctgatgcc gtgctggaac tccacatggc gaatgaaacc
      481 tgcccgaata atcttgcacc aacaacgtct accacgctga cgatggcgat cggcgatgcg
      541 ctggcgattg ccatgatcca ccaacgcaaa tttatgccga atgattttgc gcgctatcac
      601 ccgggcggtt cattaggacg tcgcctgctg acccgcgttg ctgatgtcat gcagcatgat
      661 gttcctgcgg tacagctgga tgcgtcattt aaaaccgtga ttcaacgtat caccaacgga
      721 tgccagggaa tggtgatggt agaagacgca gaaggtgggc tagcgggcat tatcaccgac
      781 ggtgacctgc gtcgctttat ggaaaaagag gattctctga catccgccac ggctgcgcag
      841 atgatgacac gtgaaccgct gacgctaccg gaagacacca tgatcattga agcggaagaa
      901 aaaatgcaaa agcaccgcgt ctcaacgtta ttggtgacca acaaggcaaa taaagtcact
      961 ggccttgtgc gcattttcga ctaattaagc aacggggtgc gagaggttag tctctcattg
     1021 agtttgcgcc ccactgaaac tttgataatc gttacatgtt gataaaagtg aagtctgccg
     1081 tatcctggat gcgtgctcgt ctgtctgcca tctcactggc agatatccaa aaacacctgg
     1141 cgaaaatcat cattctggca ccgatggcgg tgctgctgat ctatctggct atcttcagcc
     1201 agcctcgcta tatgagcgag tcgaaagtcg ccattaaacg ctcggatgat ttaaacagcg
     1261 gcagcctgaa ttttggtctg cttctgggtg cctctaaccc cagttccgca gaagatgcgt
     1321 tgtatctgaa agagtacatc aactcgccgg atatgctggc ggcgctggat aagcaactaa
     1381 attttcgtga agcgtttagc cacagcgggc tcgattttct taatcatctt agcaaggatg
     1441 aaaccgcaga aggcttcctg aagtactaca aggaccgtat caacgtctcg tatgacgata
     1501 aaaccggatt actgaatatt cagacgcagg gctttagccc ggaatttgcg cttaagttta
     1561 accaaaccgt gctgaaagag tcagagcgct ttatcaatga gatgtcacat cgcatcgcgc
     1621 gtgaccagct tgcctttgca gaaacggaga tggaaaaggc acgccagcgt ctggacgcca
     1681 gcaaagcgga attgctctct tatcaagaca acaacaacgt tctggatcca caggcacagg
     1741 cacaggcggc gagcacgtta gtgaatacgc tgatgggcca gaagatccag atggaagcgg
     1801 acctgcggaa cttgctgacg tatctgcgtg aggacgcccc gcaagttgtg agtgcgcgta
     1861 atgcgattca gtcattgcag gcacaaattg acgaagaaaa aagcaaaatc acagcgccgc
     1921 agggtgacaa gctaaaccgt atggcggtgg attttgaaga aatcaaatca aaagtagagt
     1981 tcaacaccga gctgtacaaa ctgaccctga cctccattga aaagacccgt gtagaagcgg
     2041 ctcgtaagct caaggtgctg tcagtgatca gttcgccaca gttgccgcag gaatcgtctt
     2101 ttccaaatat cccttatttg atcgcctgct ggttactggt gtgctgcctg ctgttcggca
     2161 ccctgaaact gttgctggct gttattgaag atcaccgaga ttaacgctgt cgctgaatga
     2221 gtttgtgatg aaattattga aatcaatttt actgattgcc gcctgtcagg ctgcgcaggc
     2281 cagcgcggcc attgatatta acgctgaccc aaacctgaca ggagcagcgc cgcttaccgg
     2341 tattctgagc ggacaaaaat cggatacgca aaacatgagc ggctttgaca ataccccgcc
     2401 gcccgcaccg ccggtggtca tgagccgcat gtttggtgct caacttttca acggcaccag
     2461 cgcggatagc ggtgcgacgg taggattcaa ccctgactat attctgaatc cgggcgatag
     2521 cattcaggtt cgcttgtggg gtgcgttcac ctttgatggt gcgttacaga ttgatcccaa
     2581 aggtaatatt ttcctgccga acgttggtcc ggtgaaagtt gctggcgtca gtaatagtca
     2641 gttaaatgcc ctggtcacat ccaaagtgaa agaagtgtac cagtccaacg tcaacgtcta
     2701 cgcctcctta ttacaggcgc agccagtaaa agtgtacgtg accggatttg tgcgtaatcc
     2761 tggtctgtat ggcggtgtga cgtctgattc gttactcaat tatctgatca aggctggcgg
     2821 cgttgatcca gagcgcggaa gttacgttga tattgtggtc aagcgcggta accgcgtgcg
     2881 ctccaacgtc aacctgtacg acttcctgct gaacggcaaa ctggggcttt cgcagttcgc
     2941 cgatggtgac accatcatcg tcgggccgcg tcagcatact ttcagcgttc agggcgatgt
     3001 ctttaacagc tacgactttg agttccgcga aagcagcatt cccgtaacgg aagcgttgag
     3061 ctgggcgcgc cctaagcctg gtgcgactca cattacgatt atgcgtaaac aggggctgca
     3121 aaaacgtagc gaatactatc cgatcagttc tgcgccaggc cgtatgttgc agaacggcga
     3181 taccttaatc gtcagcactg accgctatgc cggcaccatt caggttcggg ttgaaggcgc
     3241 gcactccggc gaacatgcca tggtattgcc ttatggttcc actatgcgtg cggttctgga
     3301 aaaagtccgc ccgaacagca tgtcgcagat gaacgcggtt cagctatatc gcccatcagt
     3361 ggctcagcgt cagaaagaga tgctgaatct ctcgctgcaa aagctggagg aagcatcact
     3421 ttccgcccag tcctccacca aagaagaagc cagcctgcga atgcaggaag cgcaattgat
     3481 cagccgcttt gtggcgaaag cgcgcaccgt ggttccgaaa ggtgaagtga tcctcaacga
     3541 atccaatatt gattctgttc tgcttgaaga tggcgacgtc atcaatattc cggagaaaac
     3601 atcgctggtt atggttcatg gcgaagtgct gttcccgaac gcggtgagct ggcagaaggg
     3661 tatgaccacc gaggattaca tcgagaaatg tggtggcctg acgcaaaaat cgggtaacgc
     3721 cagaattatc gtcattcgtc agaacggtgc ggcagtcaac gctgaagatg tggattcact
     3781 caagcctggc gatgagatta tggttctgcc gaaatatgaa tcgaaaaaca ttgaagttac
     3841 ccgtggtatt tccaccatcc tctatcagct ggcggtgggt gcaaaagtga ttctgtcttt
     3901 gtaaggggtt gagatgagca aagcagttat tgtcattccg gctcgctatg gctcctcgcg
     3961 cctgccgggt aagccactgc tcgatattgt tggtaaaccg atgatccagc atgtttacga
     4021 gcgtgcgtta caggtggcgg gcgttgcgga agtttgggtg gcaacagacg acccgcgtgt
     4081 tgaaaaggcc gtgcaggcat ttggcgggaa agccatcatg acgcgcaacg atcatgaatc
     4141 cggcaccgat cggctggtcg aggtaatgca tacggtggaa gcagatatct acattaactt
     4201 gcagggcgat gaaccaatga ttcgcccgcg ggatgtagaa acgctgctac aaggaatgcg
     4261 tgacgatccc gcgttgccag tggcaacgct atgccacgcg atttctgccg aagaagcggc
     4321 cgagccaagc acggtaaaag tggttgtgaa tacccgccag gatgcacttt atttcagccg
     4381 ttcgccgatt ccgtatccgc gtaatgctga aaaagcgcgc tacctgaaac acgtcggtat
     4441 ttatgcttat cgtcgtgatg tgctgcaaaa ctacagccag ttaccggagt ccatgccgga
     4501 gcaggcagaa tcactggagc agctgcggtt gatgagcgcg gggatcaaca tccgcacatt
     4561 tgaggttgcc gcaaccggtc cgggcgtcga caccccagca tgcctggaaa aagtgtgcgc
     4621 cctgatggca cagaaactgg ctgaaaacgc atgattggca ttttctcgtc cggtatctgg
     4681 cgtattccgc atctggagaa atttctggcg caaccgtgcc agaaactttc tctgctgcgc
     4741 cctgttccgc aagaagttga tgctatcgcc gtgtggggac atcgtcccag cgcggcgaaa
     4801 ccagtcgcca tcgccaaagc agcgggaaaa cccgtcattc gtctggaaga tggatttgtg
     4861 cgttcgctgg atcttggcgt caatggcgag ccgccgcttt ctctggtggt ggatgattgt
     4921 ggcatttact acgatgccag caagccttca gcactggaga aactggtaca ggataaagcc
     4981 ggaaatacag ctctgataag ccaggccaga gaagcgatgc acaccatcgt gaccggggat
     5041 ttgtcgaaat ataacctggc acctgcgttt gtggctgatg agtcagaacg ttcagacatc
     5101 gttctggttg tcgatcagac atttaatgat atgtcagtga cgtatggcaa tgctggcccg
     5161 catgagtttg ccgccatgct ggaagccgcg atggcggaaa atcctcaagc cgaaatttgg
     5221 gtgaaggtgc acccagatgt actggaagga aagaaaacag gttatttcgc cgatctgcgc
     5281 gccacgcaac gagtacgttt aattgccgag aatgtcagcc cgcagtcgct gttgcgacac
     5341 gtttcccggg tttacgtcgt gacctcccag tacggctttg aagccttgct ggcaggaaaa
     5401 ccagtaacat gtttcggcca gccctggtat gcaggttggg gcttaaccga cgatcgtcat
     5461 ccgcagtccg ctttgttatc tgcccgacgc ggttctgcca cgctggagga actttttgcc
     5521 gctgcatacc tgcgttactg tcgctatatc gatccgcaaa cgggagaagt aagcgatcta
     5581 tttaccgtgc tgcaatggct gcaattacag cgcaaccatc agcagcagcg taatggttat
     5641 ttatgggcgc caggcttaac gctgtggaag tcagcgatcc tgaaaccttt cttgcaaacg
     5701 gcaacaaacc ggctgagttt ttcacgtcgc tgtactgcgg cgagcgcctg cgtggtatgg
     5761 ggtgtaaagg gagaacagca atggcgagcc gaagcgcagc gaaaatcact gccgttatgg
     5821 cgaatggaag atggttttct gcgttcatcc ggacttggct ctgacctgct gccgccgcta
     5881 tcgctggtgc tggataaacg cggtatttac tatgacgcca cgcgccccag cgatctggaa
     5941 gtgctgctga atcatagcca gctaacgctg gcgcagaaga tgcgagctga aaaattacgc
     6001 cagcgactgg ttgaaagcaa actgagtaag tacaacctgg gagcagattt ctctctacca
     6061 gccgaagcca aagataaaaa agttatcctg gtgccaggtc aggtagagga cgatgcctct
     6121 attaaaacag gcactgtctc gattaagagc aaccttgagt tattacgcac agtacgcgag
     6181 cgcaatccgc acgcctacat tatttataaa ccgcacccgg atgtattagt ggggaatcgc
     6241 aagggcgata ttccggcaga acagattgct gaacttgctg attatcaggc actggacgca
     6301 gatattattc aatgcattca gcgcgcagat gaagtgcaca ccatgacatc attatccggg
     6361 tttgaagcgt tattacatgg caagcaagtt cattgttacg gcctgccctt ctatgccggt
     6421 tggggtttaa ccgttgatga acatcactgc ccgcgccgcg agcaaaaatt aacgttagca
     6481 gatttgatct atcaggcgtt gattgtttat ccaacctata tccacccaac acggctacaa
     6541 cctattacgg tagaagaagc tgcggaatat ttaatcaaga cgccgcgcaa gccgatgttt
     6601 attacccgaa aaaaagcggg gcgagtaata cgttattacc gcaaattaat tatgttctgc
     6661 aaggtcagat ttggctaaac aatttcatag ttgactatta cacaaattat ttatgcaagg
     6721 taatgcacta accgttttat tatccggtaa aaaatatctg ctattgcagg ggccgatggg
     6781 accttttttc aatgacgtcg ccgaatggtt agagtcatta ggacgtaacg ctgtgaatgt
     6841 tgtattcaac ggtggggatc gtttttactg ccgccatcga caatacctgg cttactacca
     6901 aacgccgaaa gagtttcccg gatggttacg ggatctccat cggcaatatg actttgatac
     6961 catcctctgc tttggtgact gccgcccatt gcacaaagaa gcaaaacgct gggcaaagtc
     7021 gaaagggatc cgctttctgg catttgagga aggatattta cgcccgcaat ttattaccgt
     7081 tgaagaaggc ggagtgaacg catattcatc gctaccgcgc gatccggatt tttatcgtaa
     7141 gttaccagat atgcctacgc cgcacgttga gaacttaaaa ccttcaacga tgaaacgtat
     7201 aggtcatgcg atgtggtatt acctgatggg ctggcattac cgccatgagt tccctcgcta
     7261 ccgccaccat aaatcgtttt ccccctggta tgaagcacgt tgctgggttc gtgcatactg
     7321 gcgcaagcaa ctttacaagg taacacagcg taaggtatta ccgaggttaa tgaacgagtt
     7381 ggaccagcgt tattatcttg ccgttttgca ggtatataac gatagccaga ttcgtaacca
     7441 cagcaattat aacgatgtgc gtgactatat taatgaagtc atgtactcat tttcgcgtaa
     7501 agcgccgaaa gaaagttatt tggtgatcaa acatcatccg atggatcgcg gtcacagact
     7561 ctatcgacca ttaattaagc ggttgagtaa ggaatatggc ttaggtgagc gcgtcattta
     7621 tgtgcacgat ctcccgatgc cggaactatt acgccatgct aaagcggtgg tgacgattaa
     7681 cagtacggcg gggatctctg cactgattca taacaaacca ctcaaagtga tgggcaatgc
     7741 cctgtacgac atcaagggct tgacgtatca agggcatttg caccagttct ggcaggccga
     7801 ttttaaacca gatatgaaac tgtttaagaa gtttcggggg tatttattga tgaagaccca
     7861 ggttaatgcg gtttattatg gggggaacac aaaaaaccgc caacataata catattaaat
     7921 ttcaagtcaa tattttttga attttaagta gctaaaaaat catttgcatc ctcttcaaag
     7981 aaaagttaca taaaggtaat aaaacatact actttaagat ttaattttac ggctggtact
     8041 gtaatagaat atagctttta ttactccccc aagaaaatcc ttttatcgtg caaagaggga
     8101 gatgttatat catccgataa tattttaaca ttatcaaaat tctttaaaat atcgaaatgg
     8161 agacgcagcg tattaatccc tttttgggat ttttcattat tacacaattt tataataata
     8221 ggtgctattg aatatgtctt acactcttta taaactaaag gtgcgtatac caaagaagat
     8281 gatgctaatc caatcaagtg ttttattttg cattttttta ttattgactc tattaaaaag
     8341 tctttttcta caataataat tatatcacga cgctttgctt tatttattgc accttgaatt
     8401 gcattaatat attttttagt ttctttaggg tgaggtttta taaaaactct tgcgttatca
     8461 gatttatcta ttctcatcaa ggtagatatt aataaatgcg catacaattc gttgtcaatc
     8521 caatatcttt gacttacata tataatatca tctctagtga tacgatattt atgtatgaga
     8581 ttatccattc ttgttgacga tttaactaat ggaatagttt taaactcaaa aatttttttt
     8641 gcatcaaatt tttcttttaa caaatttgga aaggtgccgt atacattttc aaatttcaaa
     8701 tctggataat ggaacaagat aattgaatta atgatccacg aataaaaatt aatatttact
     8761 agtggttttt ttttctctgt tttatacgtc cctgtcccct cttcaattaa tgaacatctt
     8821 atattgtttt ttttgaacaa ccatatgaag attgcataat gttgtgcata gctcataaaa
     8881 taagcatctt tagcttttga atttaaaact atatatttat atacattata aaaccatata
     8941 acttttttaa gcgaaaatgt atttggctct gttggtataa atagtagttt gtatgaagaa
     9001 aacaactttt tattcatttg ctcaagaatt aatcttggca tttcagtgtt ttttttagtt
     9061 gccaagacga taagaaaatt actacgccta ctaaattttt gtataaaaag ctctgctttc
     9121 tttaattgcc ctaaatgagc aacaataaaa atatttttac tttttatttt taacggtgat
     9181 agtagctctt cagccttgtt tttagaatta aaaaaccatg agtcacggaa aaacccaatt
     9241 ggatttacaa ataatttcct caacttcttt aaactagcat caaatatcat ctttcttata
     9301 ccttaataaa actttatgga tttttttcaa tgtttttatt ttttttggcc ctaatgagaa
     9361 aaagaaattt aaaaagatca aaaacaatat aatcaatgtc gatcttttaa ttccaaaagt
     9421 cctaacatta acatttggcg gcatttttaa aatatcattc aaaatcttat tttgaatatc
     9481 cctagagtaa taaaggttct cctccatcca ttttttttta atacgtggaa tgtgtttaaa
     9541 atttaactta ttatagtcaa caataagtcc agatcttgaa aaataacatt gtccattgca
     9601 ggtatgatta aatccattta ttaaataaac cttgatttta ttagccagac aatatattgc
     9661 gaccgaagaa gatattgtaa tacaatattc aactttagaa attaaagaat caatattatc
     9721 atcgctaaat gttatatttt tcaaatcgaa gcgctcaatg tattccttaa tatcaaaaac
     9781 aattgagtct ggtgacataa gaggatttcg agttttaaaa ataaaattca tatgcggatg
     9841 tttctgaatt actctaatca aaaaacgtgc atattctatc ctctttattg gcgtcaaggg
     9901 ctcattaact tgatcaacga taagacaaga attctcactt tttgcattga tattgacatt
     9961 tttaatccat ggtggaccaa aaagaattcc attaaaagga acccctatta ttttacaaat
    10021 ttttttatac gttttaatat ctttagggga gttaataagt aaatagtgac tattagattt
    10081 tgaaataaaa gcttctatct gagaagtaag gacaatgcca ggaaaacaag atataattat
    10141 tgtatttttt gttcttagcg tattaacaat ttcatgaagg tccttccctc ctgttgaaca
    10201 aaaaataaac tcaatattaa taaaagtgaa aaaataaaca aagtcatcag gatagaatac
    10261 aactatcttt tcatctaaat ttttttttac aaactcttgt agagaattat ccaaacagca
    10321 aaaaaataaa tcatatgaat caacatctat ctgctcaaaa atatttttca aagctaaaaa
    10381 atgagaatca taacgaaaga caaaacaaag cacttttttt ctagtcataa ctggtggtac
    10441 attccgggat gtcataaaaa tctttgtaat ctttattttt tgatttaata aaatccttaa
    10501 taattctaac agcattaatt aaagcatttt ctttaaaata aggattacta gatgactgta
    10561 caacactaat aaatttttca tcttgagata tatttatccc tctgacgatt gcattttttt
    10621 caacgggtac atctattaca ctggctccac gaacacggcc tttctgccta tcaccaatgt
    10681 taatcgttgc aacttttaaa gatggaacct caattaaacc agaggaggag ttcccaatta
    10741 gcccacaaga gtatttaatc attgccaaat aatcttctga acgaatagaa atcaaatatc
    10801 tgaacttata ctctttgcaa aaatatttta ctttcctctg aattatatca gaaccagtgt
    10861 cagcgttact gccaataaaa ataaagtcgt gagtattttt aaaaaaataa atcgctgaca
    10921 ataactcatc tatttgatca ttaaccgact gcgtggaaag tgtttcagga tggaatacta
    10981 caacaaagta ccgttttaac agtgaaccat attttagttc caactcctgc ttatttggta
    11041 aatgcaatga aagagcattt tctgcaccaa gagaaccaat attaaacaca ctactaggct
    11101 tttcacctag ctgaattact cgttttttat actcttctgt agaagtaaga tggagtttac
    11161 tcattttagt aattgaatgt ctaataaact catcataatt agctaatgtc ttttcaccac
    11221 catgaatatg aattaatgga atattatgca ttgatgctgc gatagcgact gaaaatattt
    11281 catatctatc gcctaaaacc ataaccgcat catatgtgtt atttgaaaaa aaatcaccaa
    11341 acgaattgag gcaaacactc attgaatgga gaatgtgagt atgtgaagtt gtattgatat
    11401 ttatatccac aaccttgata atattaaaat tatcttgttc tataatatgt attgtatttc
    11461 catacgcatt atcacaatgc attcctgtaa ctgccaaatc aagctgtatt tctggagttt
    11521 ctcttagcat tgtcaaaagt ctccgaacta ttccatattc agctctagat ccagttacgt
    11581 ataatatttt tttcatttaa caatctccgc tatttcgttt tctaataccg tatacccatt
    11641 actattaaaa tgtaagccat cataagtata cattttattt agattgccat aagagtcttt
    11701 taaaacgtga tccaagctaa taaacttcgc aaaatctaca ttctctcgaa gataagaatt
    11761 taaatcatta attattctat tatctcgatc aatacgccca aaaacaggag gaaccgacaa
    11821 taaataaatt tttgatgcag catttttctt ctttatatac tggcatgttt tcttcaaata
    11881 ccacaatgtg tcttcttttt tccaatcact aacaactata tcattagttc caaaaaagat
    11941 gaaaacaaac tctccgaaat taacaatcag ctctttctca ataatatatt catagtactc
    12001 cttcgagttt ataccagcga tacctaagtt attaacttct atatcattta ttttttttac
    12061 gtcccaataa tcaaacagcg agtgtccaat taaagttata tcacttacac tatcaaattc
    12121 atttcgtttc tcattgattc tattatgtat gttttgataa agtatttttt gtctattttt
    12181 tttttgctga atggtaattg caagttcgaa atccattcta tcatctatat ctaaagagct
    12241 ttccttatcc ataatataag ctagtgaata gcgaccaaaa aaatgctttg tatgaagata
    12301 atgctgctta ttagctataa atatagctcc attcggatga tattctacta ttgagtttcg
    12361 attatattta ctataatcaa ggtcaaaaaa agacagtgtc gagtaatcat ctaatggtct
    12421 aattatttgt gatggcttat tgcttctagt aacagaaaca acacattggt atttttctaa
    12481 agtttgatat aactttacag cctcaataat atgggtcgaa tctctaaagg gtgaagttgg
    12541 ttgtaataaa gcaaagttct cataatcagt atatatactt aacgcatgtt ttataaattc
    12601 aaatgatgat gctttatcag ttgctagttc ttccggtctc agcaaaaaat cagcgccata
    12661 tgactctgct attgctccat actgttcgga gtcagttgtc acaattactt tctcaaacat
    12721 ttctgactgc aaggcagctt caattgtata agcaagaaga ggtttatcta tcagcatcaa
    12781 agcattttta tttctcaacc ctttagatcc actacgggct ggaattatcg caataatttt
    12841 tgttctcatt attccccctg atttttgaat tcgctatgaa ttattaattc atctggaata
    12901 aagtcttgct ctgcaatttt acccaataaa ttataccact ccatcggact gataccatta
    12961 ccaggtcttt ttgttgttat atttttttct gaaaaaacct cacctttttt tatctctgtt
    13021 ttagctataa tagactttct tgctacgatt ttattcttcc tttctgaagc tgtaaccact
    13081 ttactatttg aacctaaaga tttttcaaca cacctgaccc caatacatag atgtttcagt
    13141 tcatcaggtt ctattgaggc caaatgatct gggccagaca tagatttatc taaagtgaaa
    13201 tgtttttcaa taaaagttat tccataaggc accgccgcaa tagctgcata aaacccgcta
    13261 gaatgatcag agaagcctat gttattctta gggaagtgtt ttttcaaatc attaatagca
    13321 ttaaggttta catcctcaaa gggcgttgga tattcagtat tgcaatgtaa tattgtaata
    13381 ttatcaaccg gaactttatt atttataaaa atagaaacag actgttttat ctcatcaata
    13441 gttgccattc ctgttgatat gattattttc ttatcaagga tcggaagctt ggctattttt
    13501 tcaagatacg gtaaattcaa taactcacct gaagggattt tccatatttt ttgtttcaaa
    13561 gatgctaaaa aatcaataga gtcttcgtca aaaggggtag aaaaaacatc taaatttaaa
    13621 ctgactgcat attccattag atggagataa tcgtcatact tcatttcaag cttttttgtc
    13681 atttctaact gagattctaa ttctcctgtg ttttttattt gatactctgc cttaggtgca
    13741 atagctgaaa ttaatttatc agctttaaat gtttggaatt ttactgcatt aacaccggcc
    13801 tctttggctt ttaatatcat ttctcttgca atatcaacac taccattatg gttgcaacca
    13861 atttcagcca cgatatatat attactcatt cattccccct aattaatctt gttggagtcc
    13921 cagcaactac aacattactt ggtatatttc taattacaac cgacccagaa ccaataatac
    13981 tctttgagcc tagcttcaac tggccattta caacagtaca gctaccaaca aaagtttctt
    14041 ctccgacaga aacatcacca ttaagtacaa cattagtaga gatattgcta cagcagccta
    14101 tttcattacc atgttcaatt aaactcctag tatttattac aacggcatca tgtattctag
    14161 tatcacggtt aagtatacac attttaccaa taaaaattcc atcacccagt ataatatttg
    14221 gtgatagaat agcagtttta tctataatgt taattaagcg tagattatgt tttcttatga
    14281 tatttaaata gtgcttccta gttgatggtt tgcctatccc aataaaataa taataattat
    14341 ccttattctc gatgtctgca atatcattac ctaatattgg atatgattga tgataaccac
    14401 ttttatattt atcgataaat cctattaact cgtaatgttt atgatttaag ctgtcaatta
    14461 tagattttga aaaaccaccc gcaccaaata ttattaactt tttactcatg atatgtctca
    14521 gttaaatatt tccaaggcat aatattggaa tgtaaccact ataggtcttt tttgtaatca
    14581 gcaatggctt ccgtaacatt tttataaaat ttaccttgcc cctctttgtt aagataaata
    14641 gcaacatcac aatactcctt taaagctcgt tcactatgtg acaccattat taaatgagat
    14701 ttttctctta ttttatcgaa tatatctgaa catttctttt taaactttgc gtctccaaca
    14761 gaagtgattt catcaataag ataatagtcg aatttaaaag ccatacttaa tccaaaagct
    14821 aaccttgacc tcatgccaga agaataagtt ttaatgggca tatcgaaata ttttccgagt
    14881 tcggaaaact ctttaacaaa atcaaccctc tcatttaact catcgcgttt tgcatatagt
    14941 cgggcaacaa atttaacatt ttcacgaccg gttaaacttc cttgaaatcc accagccaat
    15001 ccaacgggcc atgaaatttt atgttctgta ataatgttcc cactatcagg acgatctata
    15061 cctccaatta tccgaagtaa agttgattta ccggctccat tttgaccaat cagggcaatg
    15121 ttatagcctt taggaaaaat aatattcaaa tttttaaaaa cataatgccg cccagtgggc
    15181 gtgcgataag acttcgtcaa attctcaatc ttaatcatga tgtcagcatt gcctcttcac
    15241 gcgttcggta taaagccaga ccgatgaaca gggtgaccag agtgaacatt gcaaggtagt
    15301 tcagactcac gccttcgctg atatagccag gcataactgc ctcgcggctt aactctacga
    15361 catgcactaa tgggttccag agtagatatg accagtattg ctttggaatc gagtgtagag
    15421 ggaacatgat gcaggagatg aaatacagcg gcttaagcag tatcggcagg accttttgca
    15481 tttcagggaa agtcttacca acgaccatga atattaagcc gacgccacat gaaagaatga
    15541 ttaacaaact ccaggtgagc acaagttgta aaaaatttgt aatttcgaaa tattcacctg
    15601 ccatccagac gataagcata agcaatatat aaacagcaac gtaaatcagt gtctcaagca
    15661 gtgctcgtgc aatgatcgta tcgatgggtt ttactggtcg ataattaaac aacccctggt
    15721 tcgcttcaat agcaccaata gaacgtttgc tgatactgct aaagataaaa aagggaatca
    15781 ggccattaag taaaaacacc gggaatgaga tgtctggcat cgtgcggtgc ataatgtaac
    15841 caaaaatgcc caacagtatc agcaaatgcg cagagggttc gagaatcgcc cacagatatc
    15901 ccagacggaa cttaccaaag cgtgttcgta tttctcgtag aaataatgcc tctacggtga
    15961 ctttctggac ttcaaatcca cttcttgcca t
//
