LOCUS       Z49241                  2500 bp    DNA     linear   BCT 18-APR-2005
DEFINITION  P.ruminicola xynA gene and xynB gene.
ACCESSION   Z49241
VERSION     Z49241.1
KEYWORDS    endo-beta-1,4-xylanase;
            exo-beta-(1,4)-xylanase/beta-(1,4)-xylosidase; exo-xylanase;
            xylanase; xynA gene; xynB gene.
SOURCE      Prevotella bryantii B14
  ORGANISM  Prevotella bryantii B14
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae;
            Prevotella.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Gasparic,A., Martin,J., Daniel,A.S. and Flint,H.J.
  TITLE     A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4:
            sequence relationships, synergistic interactions, and oxygen
            sensitivity of a novel enzyme with exoxylanase and
            beta-(1,4)-xylosidase activities
  JOURNAL   Appl. Environ. Microbiol. 61 (8), 2958-2964 (1995)
   PUBMED   7487028
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Flint,H.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-1995) Flint H. J., Rowett Research Institute,
            Nutrition Division, Greenburn Road, Bucksburn, Aberdeen, United
            Kingdom, AB2 9SB
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Prevotella bryantii B14"
                     /mol_type="genomic DNA"
                     /strain="B14"
                     /type_material="type strain of Prevotella bryantii"
                     /db_xref="taxon:752555"
                     /clone="5/4a"
                     /clone_lib="subclone in pUC18 derived from lambda EMBL3
                     clone 5"
                     /germline=""
     gene            142..1251
                     /gene="xynA"
     CDS             142..1251
                     /gene="xynA"
                     /function="xylan hydrolysis"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="belongs to family F (10) of glycoside hydrolases"
                     /codon_start=1
                     /transl_table=11
                     /product="GH10"
                     /protein_id="CAA89207.1"
                     /db_xref="GOA:P48789"
                     /db_xref="InterPro:IPR001000"
                     /db_xref="InterPro:IPR013781"
                     /db_xref="InterPro:IPR017853"
                     /db_xref="UniProtKB/Swiss-Prot:P48789"
                     /translation="MRKLTQFCLGLMLLPIAAVAQNQPTMKDVLGKYFLVGTALNSHQI
                     WTHDPKIVHAITDNFNSVVAENCMKGEIIHPEEDYYDWHDADQLVKFAEQHKMTVHGHC
                     LVWHSQAPKWMFTDKEGKEVTREVLIDRMYHHITNVVKRYKGKIKGWDVVNEAILDNGE
                     YRQSPYYKIIGPDFIKLAFIFAHQADPDAELYYNDYSMSIPAKRNAVVKLVKELKAAGC
                     RIDAVGMQSHNGFNYPNLEDYENSIKAFIAAGVDVQFTELDVNMLPNPKSFGGAEISQN
                     YKYNKELNPYVNGLTKAAQKTFDQQYLSFFKIYRKYVDHIKRVTVWGVDDGSSWLNGWP
                     VPGRTNYGLLIDRNYKVKPVVKEIIKLYE"
     gene            1334..2293
                     /gene="xynB"
     CDS             1334..2293
                     /gene="xynB"
                     /function="xylan hydrolysis"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="belongs to a newly identified family of glycoside
                     hydrolases that also includes Bacteroides ovatus xsa (67.8%
                     identical amino acids) and C.stercorarium xylA (30.7%
                     identical amino acids)."
                     /codon_start=1
                     /transl_table=11
                     /product="GH43_1"
                     /protein_id="CAA89208.1"
                     /db_xref="GOA:P48791"
                     /db_xref="InterPro:IPR006710"
                     /db_xref="UniProtKB/Swiss-Prot:P48791"
                     /translation="MKAKYVFPSDYMADPAANVFDGKLYIYPSHDYDSGECFDDDGGHF
                     QMKDYHVLCIDGDPMEQDAKDCGKQFGIEDIPWVEKQLWDNDCVEKDGKYYLIYSAKDY
                     TGVFHLGVAVADKPEGPFVPEADPIRGSYSIDPCVFKDDDGEIYVYFGGIWGGQLQWYK
                     DNKMLKAEHLPEGKEDPLPSRVARMTGDVKQFAEAPRAVIIVDETGKPLPADDPHRFFE
                     ASWMHKYNGKYYFSYSTGDTHLLCYAVGDNPYGPFTYQGVILEPVVGWTTHHSIVEYKG
                     KWYLFHHDCVPSNDTTWLRSLKVAELEYDAEGHIKTVK"
ORIGIN
        1 ctgcagccct acttcttttg gccatgttta ttatttcaaa gtatgaactg acagatgaga
       61 agatagacga aatcaatagg gcgatagaaa tgaaaaatca gcaaaactaa cctatatata
      121 cataaattaa tagtagaaaa aatgagaaag ctaacacaat tttgccttgg ccttatgttg
      181 ctgccaatag cagcagttgc tcaaaatcag cctacgatga aggatgtctt aggtaaatac
      241 ttcttggtcg gaacagcact taatagtcat cagatttgga cgcatgatcc caaaatcgtt
      301 catgctataa ctgataattt taattcggtt gtcgctgaaa attgtatgaa aggtgagatt
      361 attcatccag aagaggatta ttatgattgg catgatgctg accagttggt taaatttgcg
      421 gaacagcata agatgacagt tcatggccac tgtttggttt ggcactcaca ggctccaaaa
      481 tggatgttta ccgataagga aggtaaagaa gttacccgtg aggtgctcat cgaccgtatg
      541 tatcatcaca ttactaatgt cgttaagcga tataaaggta aaatcaaggg ttgggatgtc
      601 gttaacgagg ctatccttga taatggtgaa tatcgtcagt ctccttatta taagatcatt
      661 ggtcctgatt ttatcaagct tgcatttatt tttgctcatc aggcagatcc tgatgcagaa
      721 ttgtattata atgactattc gatgtctatt cctgctaagc gtaatgctgt agtcaaactg
      781 gttaaggagt tgaaagctgc aggatgtcgt attgatgctg taggtatgca gagccataac
      841 ggttttaact atcctaatct tgaggattat gaaaattcta tcaaggcttt cattgctgca
      901 ggtgtagatg ttcagtttac agaactcgat gtcaatatgc tacctaatcc taagagcttt
      961 ggtggtgcag agattagcca gaactataag tataataagg aacttaatcc atatgtaaat
     1021 gggttgacta aagctgctca gaagactttc gatcagcagt atctgtcatt ctttaagatt
     1081 tatcgtaagt atgtagatca tattaagcgt gtaaccgttt ggggtgtgga cgacggaagc
     1141 agctggctga atggttggcc tgtgcctggt cgtaccaact atggtctgct tatcgaccgc
     1201 aactacaagg taaaacctgt ggttaaagaa attatcaaac tttatgagta aagcatggct
     1261 gctctttgaa tagggtgata ttgctatact caaagagcat tccgatttgt taattctaaa
     1321 tatttataag caaatgaagg caaaatacgt gtttccaagt gattatatgg cagaccctgc
     1381 tgccaacgtg tttgatggta aactctatat ctatcctagt cacgactatg atagtggtga
     1441 gtgttttgat gatgatggtg gtcatttcca gatgaaagac tatcatgtac tctgtattga
     1501 tggtgatcca atggaacagg atgccaagga ctgtggtaaa cagtttggta tcgaggatat
     1561 cccatgggta gagaagcagt tgtgggataa cgactgtgta gagaaggatg gtaaatacta
     1621 tcttatctat agtgccaagg attataccgg tgtgtttcat cttggtgtag cagtagccga
     1681 taaacctgag ggaccatttg tgccagaagc agatcctatt cgtggttcgt atagtatcga
     1741 cccatgcgtt ttcaaggatg atgatggtga aatttatgtg tattttggtg gtatctgggg
     1801 tggtcagctc caatggtaca aggataataa gatgctaaag gctgaacact tgcctgaggg
     1861 taaggaagat ccacttcctt ctagagttgc tcgtatgacc ggtgatgtta agcagtttgc
     1921 tgaggctcca cgtgctgtca ttatcgtaga cgagacaggt aaacctttac cagcagacga
     1981 tcctcaccgt ttcttcgagg cttcatggat gcacaaatat aatggaaagt attattttag
     2041 ttatagtact ggagatacac atttactctg ctacgctgta ggcgataatc cttatggtcc
     2101 atttacttac cagggtgtta ttcttgaacc agttgtaggt tggactactc atcattctat
     2161 tgtagaatat aaaggcaagt ggtatctttt ccatcacgac tgtgtacctt ctaatgacac
     2221 tacttggtta cgttctttga aggtagctga gttggaatat gacgctgaag gtcatatcaa
     2281 gacagtaaaa taaagattca tatttcttcc tgaaagtttt tttgaaacct aaaatataat
     2341 aagcaaatct ttaataataa tataaacgca agcgtgcgaa tatgagcgta aaatggggga
     2401 ctcttcacgg agccccccat tattttttcc ctgtttattc tttatttctg atgttctcgg
     2461 atatagtctc taggactcat accgtacata ctcttaaaca
//
