LOCUS       KY574574               15833 bp    DNA     linear   BCT 19-SEP-2017
DEFINITION  Escherichia albertii strain SP140724 O-antigen biosynthesis gene
            cluster, partial sequence.
ACCESSION   KY574574
VERSION     KY574574.1
KEYWORDS    .
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Naumenko,O.I., Zheng,H., Senchenkova,S.N., Wang,H., Li,Q.,
            Shashkov,A.S., Wang,J., Knirel,Y.A. and Xiong,Y.
  TITLE     Structures and gene clusters of the O-antigens of Escherichia
            albertii O3, O4, O6, and O7
  JOURNAL   Carbohydr. Res. 449, 17-22 (2017)
   PUBMED   28672166
REFERENCE   2  (bases 1 to 15833)
  AUTHORS   Zheng,H. and Xiong,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2017) State Key Laboratory for Infectious Disease
            Prevention and Control, National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, P.O.Box 5, Changping, Beijing 102206, P.R. China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SOAPdenovo v. 1.04
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="SP140724"
                     /serotype="O3"
                     /db_xref="taxon:208962"
     misc_feature    <1..>15833
                     /note="O-antigen biosynthesis gene cluster"
     CDS             1..894
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase GalF"
                     /protein_id="ARO73136.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIV
                     AAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNVRQ
                     GEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVL
                     AKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIW
                     PELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNL
                     KEGAKFRKGIEKLLSE"
     CDS             1297..2337
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="ARO73137.1"
                     /translation="MKIAFVGYNPGKIGGIEAFSRNMKKDIFPESEFVYEYDEIGTFKV
                     NDCVPVLKRNILNRVLNRLSNGYFTKRKIKAKLEEKQYDIIILNSPKYLDISPDLSKTV
                     LVQHTTLDNWWKSKYNFGCNKKLAELIRKVGKIVALSEMEGQAIKRFFCVNDSRVRVIN
                     FSSSIPFLRARKESGKKLVMLARFQNEIKRIDLVIEAMDELPDYILNVYGDGKDRDYLL
                     ALAKDRKNVKIHASITDKVSILDQHNIYILSSEFEGYPVSVIEAISRKLPVVVRNTFYS
                     ASELVEGNGVLLGRTWDKKEFCKAIRSCHENYEQYSNNSESHFYKYDPNTIKTKWVNLI
                     QELEHA"
     CDS             2330..3334
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="ARO73138.1"
                     /translation="MPNDIDVIITWVDGNDPDWIKEYISYKGCKPGRFRDIGVLKYIFR
                     GLEQNMPWVRKIHLVTNGQIPVWLNLKHPKIFFHSHDDFFIYKDALPVFNSNAIELNFQ
                     SIPDLAEKFIIFNDDMLVLKPVPEERFFENELPVDYCKLSYPRKGRLYKWLRPQNYLAC
                     ALINNNYLYLKNTHVNQINKKHLYSKNYNLRTNISNFVFSLYRKVKWFEVYHHPQPHLK
                     STWEDKSLESRNVAVRNTIYTKFRSSSDVSHYLYRYQNLLNNKFYPKQYNDHCSIYVKS
                     VDSFINNIDKYFSNVFLCICEDENMENAEFIKLKKTLTKELEIMFPNKCSYEL"
     CDS             3399..4436
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q6MMD5|9.B.18.2.1"
                     /protein_id="ARO73139.1"
                     /translation="MFNSKVLLITGGTGSFGNAVLKRFLKTDIKEIRIFSRDEKKQDDM
                     RKFYRSEKLKFYIGDVRDYQSISNAMRGVDYVYHAAALKQVPSCEFYPLEAVKTNVLGT
                     ENVLEAAINHSVKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKGRNLNKSDTVICCTR
                     YGNVMASRGSVIPLFIRQAVNGQPITVTDPCMTRFMMTLDDAVDLVLHAFKYGENGDTF
                     VQKAPAATIDILVKSLLSFLNKENHPVNIIGSRHGEKQFEALCSREEMLVAEDQGRYYR
                     IPADNRDLNYSKYFEVGEKDLHLVQDYNSNNTEQLDVEGMKNLLRKLDFMREIEAGNIV
                     IPEGV"
     CDS             4439..5317
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="ARO73140.1"
                     /translation="MRILILGASGMLGYSLYANLSDYSAFEVFGTVRDVTGREHFYTRP
                     KGKIIQNVDILDLTKLERIIAKLKPDIVINCVGLIKQLSISKQYTSAIKINALLPHELA
                     EICCSHGAKLIHFSTDCVFDGKKGNYKEDDFPTAIDLYGRAKHMGEVCYGGHITLRTSI
                     IGHELVSSVSLVDWFLSQKQEVKGFSKAIFSGVPTAYIAKLLAEKIIPNTSLRGLYHLS
                     AFPINKYDLLTKISKAYNKDIIIHESDELIIDRSLNSEFLREKIEFIPPNWDELINYMN
                     SDYEKWYKLCK"
     CDS             5296..6447
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="ARO73141.1"
                     /translation="MVQTMQMNNKMKIVTIVGTRPEIIRLSRVISRLDECSEHILVHTG
                     QNYDYELNQIFFNDLNIRMPDVFLECAGNTSAETIANVISKSDQLLGELKPEAVLILGD
                     TNSALSAISAKRRKIPIFHMEAGNRCFDLRVPEEINRKIVDHISDINLPYSAIAREHLL
                     REGLNPDLIIKTGSPMDEVLSYYADKIDSSLSLQQLNVEENKYFLVSVHREENVDYDVN
                     IHKFVVLLNTLAEKYSLPIIVSTHPRTRKKIESLKLNFSPLVQLMRPLGFSDYVKLQKN
                     AKVVLSDSGTITEESSILDFPAINIREAHERPEGFEEASVMFTGMNVERILQVIEILEK
                     QTKGDKRILRKVYDYDAPNVSEKIVRIIYSYTDYVNRVVWKKY"
     CDS             6447..7664
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="ARO73142.1"
                     /translation="MHFALIIDDYLPSSTRVASKMFHELACSFVNSGHRVTVITPYHGN
                     GSRLKKKYLDGVEIWYFKNPRVKDVGKIKRAIAETVLSLNAWMAIRYETEVRTFDAIVY
                     YSPSIFFGPLIRKIKSQSKCFSYLILRDIFPQWSVDAGLIKAGSLIEKYFRFFEKYNYS
                     QADYIGLMSKKNVELFNSKYSGYSTGVLHNWARFERIYYEPKYRQSLGLNNKVIFLYGG
                     NIGHAQDMRNLLRLVRRMLPYDEAHFLFVGQGDEVELVNSLSKQWELKNLTYLPALTQS
                     EFKILLSEIDVGLFSLSKHHTAYNFPGKILGYMVNSIPILGSVNDGNDLMSLINDANAG
                     MIYINGNDEELFQAALALLKDKNLRHNMGECALSLLRKEFDVEGVAVEILRKVTEFYEE
                     NRSENN"
     CDS             7636..8040
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="ARO73143.1"
                     /translation="MKKIDLRIINRLFNQAKDDERKRAHYLLHKSYEEKVQRLLIAFLK
                     DSYVEPHFHRNKNQWEMFIVLNGIFELTKYSEQGTIVEQMIIEAGGDVLAVEIYPGEIH
                     SVKCLSEKGLLMEIKEGPFNVLEAKEYLIK"
     CDS             8141..9388
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ARO73144.1"
                     /translation="MNISLMKNSITFKHYELSSGLYIMITIYSLMVNIYLSIPFLLYGL
                     IKNKKGTSLFIALFFGIMGYKFSPPMEFDLYRHYESYEYYLQYNEFLYRIKDLYLEGLF
                     FIGKSLSLKKEFLYFTSIVIYYTCILTIVLKLRNRTPLKGVYFFFIFTLFLLTNTIIEI
                     TGLRFTTALGFASLFIYYRFIELRKGLSYFFILIAVLSHFSVIILPVVIIVLRLLHKLF
                     NSRFCSIIILLTSVIAGLYFVESVVAFSLLSIEQIFNVYIGAETYTSGLWGADRVTLHG
                     FSQTGIIFEKIKLGISIVIPMIISITFILDRDYGRDELAKLFVACSAIFFIFIPFDTVY
                     MRYQYLLIIIALIIFAKKDYKFKYIDGQFIGISMILLKILMQITVGLLSTYIYYMRGLN
                     INIIDMNLVAVLFNIL"
     CDS             9410..10669
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P0AAA7|2.A.66.2.3"
                     /protein_id="ARO73145.1"
                     /translation="MFKTAILSLISTIFKLLSALIINKVISIFVGPAGIALIGQFQNLI
                     QMVNTLSQGGMLSGVTKYTAEYNKHSPENIEKLWSTAFKIIVLLSIILGFLLIVLSKAL
                     SSYLFGTQDYYIFIRFLGCSTIFTSLNGFVLAVINGLKKIKLYVLINIYQSLISLMLST
                     LLVMLYGLKGAFLALILNQSIIFVTLLFIFNKNGGREKVNKFIGRASTEELKKLFSFSL
                     MAITSAIVGPVSLMLIRNFIQENINLNASGYWQSMWYISSMYLLVITTTLSIYYLPRLS
                     ELVSTDELKVELIKGIKIIVPLVATASFLVYLLKDFIISILFTPEFSNMRDLFRWQVCG
                     DVLKITAWLFSYVLIAKAQSKAFIILEVINFSLFTVLSIVMIHTYGLIGGSYGYFFSNL
                     IYLILVSIVVKVRFFKNYKS"
                     /old_product="Wzx"
     CDS             10688..11815
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="ARO73146.1"
                     /translation="MAKYIFIARNTVPFANPAGLVVYRIAKALSDLGHDVDIFSLNKPT
                     GEQKIPEWLTDGDKIHIKSFDLTKKNNISVYLRFILTLFSKIKKELKENKFIYIVTHTN
                     PLYTHWVGFLCKLFFYKRVKWISSFTDPYVRSPFSGYAKFTVGTGIRILEQKLSFYFSE
                     KIIFVTDTMKNYICRENKSALNKSIVIPFFYLKDWEKRILNCNADRQSRDDQRLIIMHA
                     GSIYGNRDASKFLNALEEHESTILFKNLGVVNQKIQYTSNNILITNPLPYGEMISYLKN
                     TDYILIIDSFFDNIKNPYMPSKVVDAMYLHKPIIGITEKNSELAVFLRKTGNISIPNDR
                     AIISSVLLKIKRKGTCDFSLYADSNLSDKFRELEC"
     CDS             11893..12891
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="ARO73147.1"
                     /translation="MILVTGGLGYIGSHTVVELLSRDYEVIILDNFSNSSPEVVAKIKA
                     ISGVEPLIFEGDIRDVNLLKKIFTDNNISDIIHFAGLKSVAESIKCPLAYYNNNVIGTL
                     TLIDCALNFKIKNFIFSSSATVYGNPETIPLDEHCNTGGTTNPYGTSKYLVERILQDIA
                     KAHSELNITILRYFNPVGAHPSGLIGESPIGVPNNLMPFISRVASGKYEYVSVYGNDYD
                     TPDGTGVRDFIHVMDLATGHVAALENQNREKNLHIYNLGAGVGYSVLDIIRKFEEVNGV
                     KINYKFSERREGDIGECWSNPSLAFKELKWKATKTLDDMVKDVWKWQKNNP"
     CDS             13018..14424
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="ARO73148.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAE
                     NPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLEKGDIIIDGGNTF
                     FQDTIRRNRELSSEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVA
                     EDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQTFTEW
                     NNGELSSYLIDITKDIFTKKDEGGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLIT
                     ESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQL
                     RAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPKIANLLLAPYFKKIADD
                     YQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDK
                     EGVFHTEWLD"
     CDS             14667..15833
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="ARO73149.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISPV
                     VDKEIQNFLQSDEIRFNATLDKKEAYRNADYVIIATPTDYDPKTNYFNTFSVESVIQDV
                     IEINPLAVMVIKSTVPVGFTAAMQTKYHTENIIFSPEFLREGKALYDNLHPSRIVIGER
                     SERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLG
                     LNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANR
                     TRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMK
                     EDSFFNSRLERDLATFKQQADIIISNRMAEELKDVADKVYTRDLFGSD"
ORIGIN
        1 atgacgaatt taaaagcagt tattcctgta gcgggacttg ggatgcatat gttgcctgcc
       61 actaaggcga tacccaaaga gatgctaccg atcgtcgaca agcccatgat ccagtacatt
      121 gttgacgaaa ttgtggctgc agggatcaaa gaaatcctcc tggtgactca tgcgtccaag
      181 aacgcggtcg aaaaccactt cgacacctca tatgaattag aatctctcct tgaactgcgc
      241 gtaaaacgcc agttgctggc agaagttcag tccatctgcc caccgggcgt taccattatg
      301 aacgtgcgtc agggcgaacc tttaggtttg ggccactcca ttctgtgtgc cagacctgcc
      361 attggcgaca atccattcgt tgtggtgctg ccggatgtcg ttattgatga cgccagcgcc
      421 gatccgctgc gttacaacct tgccgccatg attgcgcgat tcaacgaaac gggtcgtagc
      481 caggttctgg caaaacgtat gccgggtgac ctttctgaat actccgtcat ccagactaaa
      541 gaaccgctgg atcgtgaagg caaagttagt cgcattgttg aatttatcga aaaacccgat
      601 cagccgcaga cgctggactc agacattatg gcggttggtc gctatgtgct ttctgcagac
      661 atttggcctg aactggaacg gactcaaccc ggtgcatggg gacgtattca gctaactgac
      721 gcaattgccg aactggcgaa aaaacagtcc gttgatgcga tgttaatgac gggtgacagc
      781 tacgactgcg gtaaaaaaat gggctacatg caggcatttg tgaagtatgg cttacgcaac
      841 ctgaaagaag gggcgaagtt ccgcaaaggg attgagaagc tgttaagcga ataaaaatct
      901 taccgggtgt aacgatcaat aagaaaatca taacggcagt gaagattcgt agcaaaagta
      961 aattgcaacg aatgttcctg ccgttgtttc tttataaaac actataataa caatgagtta
     1021 gcaataagat tttagtcaaa gttttccagg attttccttg tttcctgagt ggattggtaa
     1081 gacaattagc gtttgaattt atgagggctt tgcgggggta ggtgcaaagt tcgtgacatc
     1141 tattgagaat ctacgcagtg cactggtagc tgttaagcca ggggcggtag cgtgctcatc
     1201 agcctcatcg tatgttgtaa cattgttaca gcatactgaa gactgtacaa ataaaaatgc
     1261 tgatactcat cagctataag aataaaggta tcaggaatga aaatagcgtt tgttggatat
     1321 aaccctggaa aaattggtgg tattgaggca ttttcgcgca atatgaagaa agatattttt
     1381 cctgagtctg agtttgtata tgaatatgat gagattggca cctttaaagt caatgactgc
     1441 gtgccagtat taaaacgaaa tatactaaat agagtattaa acaggttatc aaacggatat
     1501 ttcacaaaaa gaaaaataaa agccaaatta gaagaaaaac aatatgatat tattatttta
     1561 aattcaccta aatacttgga tatatcacca gatttaagca aaacagttct agttcaacat
     1621 acaacactag ataactggtg gaaatcaaag tataactttg gatgtaataa aaaattagcg
     1681 gaactaatta gaaaagttgg taaaatagtc gctttatctg aaatggaagg ccaggcaata
     1741 aaaagatttt tctgtgttaa tgatagcaga gttcgcgtga taaacttttc atccagtatt
     1801 cctttcttgc gtgctagaaa ggaatctggc aaaaagcttg ttatgttggc aagatttcaa
     1861 aatgaaataa aaagaatcga tcttgttatt gaagctatgg atgagttacc agattatata
     1921 ttaaatgtat atggagacgg aaaagatcgt gattatctat tagctttagc gaaagataga
     1981 aaaaatgtaa aaatacatgc tagcataacc gataaagttt caatattaga tcaacataat
     2041 atttatatac tgagtagtga gtttgaaggt tatcctgtaa gtgttataga agcgatttct
     2101 agaaaattac cagtggtagt tagaaataca ttttattcag catcagaact tgtggaaggt
     2161 aatggtgtat tactcggaag aacctgggac aaaaaagagt tttgtaaagc aattcgttct
     2221 tgccatgaaa attatgagca atattctaat aattcagaga gccattttta taagtatgat
     2281 ccgaatacaa taaaaactaa atgggttaat ttgattcagg agcttgaaca tgcctaatga
     2341 tatcgatgtg atcattacat gggttgatgg taacgatcct gattggatta aagaatatat
     2401 tagctataag ggatgtaaac cgggcagatt tcgagatata ggagtgttga aatatatatt
     2461 tagagggctc gaacaaaata tgccatgggt acgtaaaatt catttggtca ctaatgggca
     2521 aattccagtc tggctgaatt taaagcatcc gaagatcttt ttccattctc acgatgattt
     2581 ttttatttac aaagatgcac ttcctgtatt taattcaaat gcaattgaat taaattttca
     2641 aagcattcca gatttagctg agaaattcat tatatttaat gatgacatgc tggttttaaa
     2701 accagtccca gaagaacgct tttttgaaaa tgagctacct gttgactatt gtaagttatc
     2761 atatcctcga aaaggtaggc tttataaatg gttgaggcca caaaattatc ttgcatgtgc
     2821 gttgattaat aataattatt tatacttaaa aaatactcat gtaaaccaaa tcaataaaaa
     2881 acatctatat agtaaaaatt ataatttaag aacaaatatc tctaactttg tattctcgtt
     2941 atatagaaaa gtaaagtggt ttgaagttta tcaccatcct caaccgcatt tgaaatcaac
     3001 ttgggaagac aaatcattag aatcgcgcaa tgttgctgta aggaatacaa tatatacgaa
     3061 atttcgatct agttctgatg ttagtcatta tttgtataga tatcaaaatt tattaaacaa
     3121 caaattttat cctaagcaat ataacgacca ctgttccata tatgtcaaaa gtgtcgattc
     3181 ctttattaat aatatagata agtatttttc aaatgttttt ctttgtatat gcgaagatga
     3241 aaatatggaa aatgcagaat ttattaagtt aaaaaaaaca ctaacaaagg aactggaaat
     3301 tatgtttcca aataaatgtt catatgagct ttaatggcta tttatgaata taatgtaaaa
     3361 catctccatt aatatttatt gtcaataata ggtttattat gtttaattcg aaagtcttat
     3421 taataactgg cggtactggt tcttttggaa atgcagtttt aaaacgtttt ctcaaaacag
     3481 atataaaaga aattcgcatt ttttctagag atgagaaaaa acaagatgat atgcgtaagt
     3541 tttatcgttc ggaaaagctc aagttctata ttggcgatgt gagagattat caaagtatct
     3601 caaacgcaat gcgtggagtt gattatgttt atcatgcagc tgcgctaaag caagttccct
     3661 cttgtgaatt ttatccactt gaagcggtta aaacgaatgt attaggaact gaaaatgttt
     3721 tagaagctgc aattaatcat tcagtaaaac gtgttgtatg tttaagtacc gataaagcag
     3781 tctatcctat aaatgcaatg ggtatatcca aggcaatgat ggaaaaagta attgtagcta
     3841 aaggtcgcaa tctaaataaa agtgatacag ttatttgctg tactcgctat ggtaatgtga
     3901 tggcatcgcg cggttctgta attcctttat tcatcagaca agcggttaat ggtcaaccga
     3961 taacagtaac tgatccttgt atgactcgtt ttatgatgac gcttgatgat gcagtggatt
     4021 tagttctaca tgctttcaaa tatggtgaaa atggtgatac atttgtacaa aaagcccctg
     4081 ctgcaactat tgatattttg gttaaatctc tcctttcttt tttaaataaa gaaaatcatc
     4141 ctgttaatat tattggttct cgccatggtg aaaaacagtt tgaagcatta tgcagtaggg
     4201 aagagatgtt ggtcgcggaa gatcaaggcc gttattatcg tatacctgct gacaatcgcg
     4261 atttaaacta ttcaaaatat tttgaagtgg gtgagaaaga ccttcactta gttcaagatt
     4321 ataattcaaa taacacagaa cagcttgatg tcgaaggaat gaaaaatctt ttaagaaagt
     4381 tagactttat gcgcgaaatc gaagctggaa acatcgtaat ccctgaagga gtgtagtaat
     4441 gagaatcctt attttggggg ctagcggaat gttaggatat agcctttatg caaatttgag
     4501 tgattattca gcttttgagg tatttggaac ggtaagagat gttactggaa gggagcattt
     4561 ttacacgaga cccaaaggaa agataataca gaatgttgat atcttagatt taactaagtt
     4621 agaacggatt attgctaaat taaaacctga tattgtgata aactgcgttg gcttaattaa
     4681 acaactatct atatcgaagc agtatacctc agcaataaaa attaacgcat tattgccaca
     4741 tgagttggct gaaatatgtt gttctcatgg ggcaaagctt atccattttt cgacggattg
     4801 cgtatttgat ggtaaaaaag gcaattataa ggaagatgat tttccaacag ccatagatct
     4861 atatggacga gctaaacata tgggagaggt ttgttatgga gggcatatta ctctcaggac
     4921 atctataatt gggcatgaat tagtgtcgtc tgtaagtcta gttgattggt ttttgagcca
     4981 gaaacaagaa gttaaggggt tctcaaaggc tatattttca ggggtaccta cagcatatat
     5041 tgcaaaatta ttagctgaaa aaataattcc gaatacatca ttaagagggt tatatcattt
     5101 atctgcattc ccaattaata aatatgattt gttaactaag atatcaaaag catataacaa
     5161 agatataata atacatgagt cagatgagtt aattattgat aggagtttaa attctgaatt
     5221 tttgagagaa aaaattgaat ttatacctcc taattgggat gaacttatta actacatgaa
     5281 ttctgattat gaaaaatggt acaaactatg caaatgaata ataaaatgaa aattgttact
     5341 attgttggaa cacggcctga aatcattagg ttatctagag taatatctag attagatgag
     5401 tgcagcgaac atatacttgt ccatactggg cagaattatg attatgaatt gaatcaaatc
     5461 ttttttaatg atttgaatat tagaatgcca gatgtttttc ttgaatgtgc aggaaatact
     5521 tcagcagaaa caattgcaaa tgtaataagc aagtccgatc aattgctggg ggaattgaag
     5581 ccggaagccg ttctcatatt aggtgacacc aacagcgcac tatctgctat atcagcaaaa
     5641 agaagaaaaa tccccatttt ccatatggaa gctggaaata gatgttttga tttaagagta
     5701 ccagaggaaa tcaatagaaa aatagtagat catatctctg atatcaattt accatatagc
     5761 gcaattgcga gagaacattt actgcgtgaa ggtctgaatc cagatcttat tatcaaaaca
     5821 ggaagtccga tggatgaagt tttgagctat tatgcagata agattgactc ttctttgtct
     5881 ctacagcaac tgaatgttga agaaaacaaa tattttcttg tgagtgtaca tcgtgaagaa
     5941 aatgttgatt atgatgtgaa tattcataag tttgttgtgc tactaaatac tttagctgag
     6001 aaatattctt taccaataat agtatctacg catccgagaa cgcgcaaaaa aattgagtca
     6061 ttaaagctta atttttcacc tttggtacaa ctaatgagac ccttgggatt tagtgattat
     6121 gttaaattac aaaaaaatgc taaggttgtc ttaagtgata gtggaacaat taccgaagaa
     6181 tcatctattt tggactttcc tgcaatcaac attcgtgaag cccatgaaag acctgaaggt
     6241 tttgaagaag catctgttat gtttactggt atgaatgttg aaagaattct tcaagttatt
     6301 gaaattttag aaaaacaaac aaagggagat aaacgaatct taagaaaagt atacgattat
     6361 gatgctccta atgtttcaga aaagatcgtt cgtataattt attcttatac tgactacgta
     6421 aatcgagttg tgtggaaaaa atattaatgc actttgctct tatcattgat gattatttgc
     6481 ctagcagtac tagagttgct tcgaaaatgt tccatgaact ggcttgttca tttgtgaata
     6541 gtggtcacag ggttacggtc ataactccat atcatggaaa tggaagtcgg cttaaaaaaa
     6601 aatatttaga tggagttgaa atttggtatt ttaaaaaccc tcgggttaaa gatgttggaa
     6661 aaataaagcg tgcaatagct gaaacagttc tctcgttaaa tgcgtggatg gctataaggt
     6721 atgagactga ggttagaact tttgatgcta ttgtttatta ttcgccatca attttttttg
     6781 gtccattaat aagaaaaatc aagtcgcagt cgaaatgttt ttcatatctg attcttagag
     6841 atattttccc tcagtggagt gttgatgcag gtttaattaa agctggatcg ttaatagaaa
     6901 aatattttcg cttctttgaa aaatataatt attcacaggc cgattacatc ggcctgatgt
     6961 ctaaaaaaaa tgttgagcta ttcaatagta aatatagtgg atactcaacg ggggttctgc
     7021 ataattgggc tagatttgaa cgtatatatt atgaaccaaa atatcgacaa tcgttgggat
     7081 taaataataa agttatattt ctctatggag gaaatattgg tcatgcccaa gatatgagaa
     7141 atttgctacg attggttcgt agaatgcttc catatgatga agctcacttt ttatttgttg
     7201 ggcaggggga tgaagttgaa cttgttaatt cattatcaaa gcagtgggaa ctaaaaaatc
     7261 taacttatct gccagcatta actcaatcag agtttaaaat tctgctctct gaaattgatg
     7321 ttgggctttt ttccctttcc aaacatcata ctgcttataa ttttcctgga aaaatattgg
     7381 gttatatggt taattcaata ccgattttag gaagtgtgaa tgatggtaat gatctaatgt
     7441 cattaattaa tgacgccaat gctggcatga tttatataaa tggtaatgat gaggagcttt
     7501 ttcaagctgc tttggccttg ttaaaagata aaaatttaag acataatatg ggagaatgtg
     7561 cgttatctct tcttagaaaa gagtttgatg tagagggggt tgctgtagaa atattaagga
     7621 aggttactga attttatgaa gaaaatagat ctgagaataa ttaatagact ttttaatcaa
     7681 gcaaaagatg atgaaagaaa aagagctcat tatcttttac ataaatctta tgaagaaaaa
     7741 gtgcagcggc tgttaattgc ttttctgaaa gatagctatg tagagcctca ttttcatagg
     7801 aataaaaatc agtgggaaat gtttattgtg cttaacggaa tatttgaatt aactaaatat
     7861 tccgaacaag gaacaatagt ggaacaaatg atcattgagg caggggggga tgttctagcc
     7921 gttgaaattt atccaggaga aatacatagt gtgaaatgtc tttctgaaaa ggggttgctt
     7981 atggaaatta aagaaggccc ttttaacgta ttagaggcaa aagaatattt aataaaataa
     8041 aagtacctga actgtcagtc tgtctatcat acattaataa ttctgataaa agtgaattta
     8101 caattagtta acgagatgca attacacact aagttatttt atgaatatat cactcatgaa
     8161 aaatagtata acatttaaac attatgagct aagttctgga ttatatatta tgattacaat
     8221 atatagtctg atggtgaata tttatcttag tattcctttt ttattatatg gtttgataaa
     8281 aaataaaaaa ggcacttcat tattcatagc tctatttttt ggcatcatgg ggtataaatt
     8341 ctctccacca atggaatttg atttatatag acattatgaa tcatatgagt attatttaca
     8401 gtataatgaa tttctttata ggataaaaga tttgtatctt gagggacttt tttttattgg
     8461 aaagagttta tcattaaaaa aagaattttt atattttacc tcgatcgtaa tatactatac
     8521 atgtatatta acaatagtat tgaaattaag aaatagaaca cctctcaaag gcgtgtactt
     8581 tttttttatc ttcacgctct ttttactgac aaacacaatt attgaaatta ctggccttag
     8641 atttaccact gcactgggat ttgcatcact ttttatatat tatagattta ttgaactgag
     8701 aaaaggatta tcctattttt ttattcttat tgctgtatta agccatttct ctgtaattat
     8761 attacctgtg gtaattattg ttctgcgatt attacacaaa ttatttaatt ctcgattttg
     8821 ctcaataata atacttttga cttcggtaat tgcagggtta tattttgttg agtcggttgt
     8881 tgctttttca cttttaagta ttgagcagat ttttaatgta tatattgggg ctgagaccta
     8941 cactagtgga ttatggggag ctgaccgggt cactttgcat ggttttagcc aaactggcat
     9001 tatatttgag aaaataaagt taggcataag tattgttatt ccaatgatta taagtattac
     9061 atttattcta gatagggatt atggtaggga cgaattagct aaattatttg ttgcatgttc
     9121 tgctattttc tttattttta ttccttttga tactgtttat atgcgctatc agtatctttt
     9181 gataataatc gcattgataa tttttgccaa aaaagactat aaatttaaat atattgatgg
     9241 tcagtttatc ggtatttcta tgattctttt gaaaatacta atgcaaatta cagtaggttt
     9301 actatctaca tacatttatt acatgcgcgg tttaaatatc aatattatcg acatgaatct
     9361 tgtggctgtt ttgtttaata ttttgtaaaa atcgaggtgg ttgttgaata tgttcaaaac
     9421 agcaatactt agtttaatat ctacgatatt taaattatta agtgcattga ttataaataa
     9481 agtcatttcc atttttgttg gacctgctgg tattgctctg attggacaat ttcaaaatct
     9541 tatccaaatg gtaaatactc tctctcaggg aggtatgtta tctggtgtga ctaaatatac
     9601 agctgagtat aataaacatt ctccggaaaa tatcgaaaag ttatggagta cagcatttaa
     9661 aataatagtt ttattatcga taatattagg ttttctttta attgttttat ctaaagcatt
     9721 atcttcttac ttgtttggaa cacaagacta ttacattttt attcggtttc ttggttgttc
     9781 tacaatattt acatcattaa atggttttgt gcttgcggtt attaatggat taaaaaaaat
     9841 aaaactttat gttttaatta atatatatca aagcctcatc tcattaatgt tatcaacact
     9901 gttagttatg ttatatggtc taaaaggggc ctttttagca ttgatactta accagtctat
     9961 aatatttgtt acccttctgt ttatttttaa taaaaatgga gggagagaaa aagtaaataa
    10021 atttattggt agggcatcta cagaagaatt aaagaaatta ttctcatttt ccttgatggc
    10081 tataacatct gcaatagttg gacctgtttc attaatgtta ataagaaatt tcatccaaga
    10141 aaatattaat cttaatgctt cgggttattg gcaatcaatg tggtacattt cttcgatgta
    10201 cctattggtg attactacaa ctctatcaat atattactta ccacgcttgt ctgagcttgt
    10261 tagtactgat gaactaaaag ttgagcttat taagggtatt aaaattatag ttcctcttgt
    10321 ggctactgct tcatttttgg tttatttgct taaggatttt attatatcga tacttttcac
    10381 ccctgagttt tcaaacatga gagatttatt tcgatggcaa gtttgtggtg atgtattgaa
    10441 gatcactgct tggctttttt cctatgtatt aatcgcaaag gctcaatcca aagcatttat
    10501 catattagag gtgataaatt ttagtttatt tacggtgttg tcgattgtta tgattcatac
    10561 ctatggactg ataggaggat catatggtta ttttttctcc aatttgattt atttaatact
    10621 tgtttctatc gttgtaaaag tccgtttttt taagaactat aaaagttaat gtttaaggtg
    10681 ataaattgtg gctaagtata tttttatagc aagaaatacg gtcccttttg caaaccctgc
    10741 cggccttgtt gtttatcgta ttgcaaaagc gctttccgat ttaggtcatg atgttgatat
    10801 cttttcttta aataagccaa caggtgaaca aaaaattcct gagtggttga cggatggtga
    10861 taaaattcat attaagtctt ttgatttaac aaaaaaaaat aacatatctg tttatttgag
    10921 gtttatttta actctttttt ctaaaataaa aaaagagtta aaagaaaata aatttatcta
    10981 tattgtgact catactaatc ctctttatac tcattgggtt ggttttttat gtaaattgtt
    11041 tttttataaa agagtcaagt ggatttcttc ttttaccgat ccatatgtaa gatctccgtt
    11101 tagtgggtat gccaaattta cagtgggaac tggtatcagg atacttgagc aaaagttatc
    11161 gttctatttt agtgaaaaaa ttatctttgt aacggacacg atgaaaaatt atatttgtcg
    11221 tgaaaacaaa agtgccttga ataaatcgat tgttattcct ttcttctatt tgaaagattg
    11281 ggaaaaaaga atacttaatt gcaatgctga tagacaaagt agagatgatc agcgattgat
    11341 tattatgcat gctggttcaa tttatgggaa tagagatgcc tctaaatttc ttaatgcgtt
    11401 agaagagcac gaatcaacaa ttttatttaa aaacttaggc gtagttaatc aaaaaattca
    11461 gtatacgagt aataatatat taattactaa ccctttacct tatggtgaga tgatttctta
    11521 tttaaaaaat actgattaca ttcttattat tgattccttt tttgataaca taaagaatcc
    11581 atatatgccg tccaaagttg tagacgcaat gtacttacat aaaccaatta tcggaataac
    11641 tgaaaaaaat tctgaattgg ctgttttttt acgcaaaact gggaatatat ctattccaaa
    11701 tgatagagca attatatctt cagtgctatt aaaaattaaa cgaaagggta cgtgtgactt
    11761 tagtctgtac gccgattcaa atctttcaga taaatttaga gagcttgaat gttaatcacc
    11821 atgctttcct tcttttctgt gagttaagaa gtaaaatgtt ttatcccata atggcagaag
    11881 cgaggagtat caatgatttt agtaacaggt ggtttaggtt atattggatc tcatactgta
    11941 gttgaattac tatctcgtga ttatgaagta attattttag ataatttttc taattcatct
    12001 cctgaagttg tggctaagat aaaggctata agtggcgttg aacctctgat ttttgaaggt
    12061 gatattagag acgttaattt actaaaaaaa atatttactg acaacaatat atccgatatt
    12121 atccattttg ctggtttaaa atcagttgct gaatctataa aatgccctct ggcatattat
    12181 aataacaatg ttattgggac tttaacatta attgattgtg cgctgaattt taaaattaaa
    12241 aactttattt ttagttcttc agctactgta tacggaaatc cagaaacaat tccattggat
    12301 gagcactgta atactggtgg aacaactaat ccttatggaa catcgaaata tctcgttgaa
    12361 cgaatccttc aagatatagc taaagcacat agtgaattaa acattacgat acttagatat
    12421 tttaatcctg taggggcgca cccatcagga ttaattggtg agtctcctat tggtgttcct
    12481 aataacttaa tgccgtttat atctcgtgta gcgagtggta aatatgaata tgtttctgta
    12541 tatggaaatg attatgatac gccggatggt actggtgtaa gggactttat ccatgtaatg
    12601 gatcttgcta cagggcatgt tgctgcatta gagaaccaga accgagaaaa aaatttgcat
    12661 atttataact taggtgctgg tgtaggatat tcggtattag atattataag aaagtttgag
    12721 gaagttaatg gtgtgaagat taattacaaa tttagtgaga gacgagaagg ggatataggt
    12781 gaatgttggt caaatcctag cttagctttc aaagaattga aatggaaagc aactaaaact
    12841 ttagatgata tggttaaaga tgtttggaaa tggcaaaaaa acaaccctta aatatatatt
    12901 tgatattgaa acataataat attgcttgtt aatgatttta ttgtgcgaac cttaatgaat
    12961 ttattacgtt gatttaatct acttgtaact cgctatagcc cctgacagga gtaaacaatg
    13021 tcaaagcaac agatcggcgt cgtcggtatg gcagtgatgg ggcgcaacct tgcgctcaac
    13081 atcgaaagcc gtggttatac cgtctctatt ttcaaccgct cccgtgaaaa aacggaagaa
    13141 gtgattgctg aaaatcctgg caaaaaactg gttccttact atacggtgaa agagttcgtt
    13201 gaatctctgg aaacgcctcg tcgcatcttg ctaatggtga aagcaggtgc aggcacggat
    13261 gctgctattg attccctaaa gccatacctt gaaaaaggcg acatcatcat tgatggtggt
    13321 aacactttct tccaggacac cattcgtcgt aaccgtgagc tttcttctga aggttttaac
    13381 ttcattggta cgggggtttc cggtggtgaa gagggcgcgc tgaaaggtcc ttctatcatg
    13441 cctggtggtc agaaagaagc ctatgaactg gttgcgccta tcctgaccaa aatcgctgcc
    13501 gtagctgaag atggtgaacc gtgcgttacc tatattggcg cagacggcgc aggtcactat
    13561 gtgaagatgg ttcacaacgg tattgaatac ggtgatatgc agttgattgc tgaagcctat
    13621 tcactgctga aaggtggtct gaatctctcc aatgaagagc tggcacagac ctttaccgag
    13681 tggaataacg gtgaactgag cagctacctg atcgatatca ccaaagatat cttcaccaaa
    13741 aaagatgaag gcggtaacta cttggttgat gtgatcctgg atgaagcggc taataaaggc
    13801 accggtaaat ggaccagtca gagtgcgctg gatctcggcg agccgctgtc gttgattacc
    13861 gagtctgtgt ttgctcgtta tatctcttct ctgaaagagc agcgtgttgc tgcgtctaaa
    13921 gttctgtctg gtcctcaggc acagccagca ggcgacaaag cggagttcat cgagaaggtt
    13981 cgccgtgcgc tgtatcttgg caaaatcgtt tcttatgctc agggcttctc tcaactgcgt
    14041 gccgcgtctg aagagtacaa ctgggatctg aactacggtg aaatcgcgaa gattttccgt
    14101 gctggttgca ttattcgtgc gcagttcttg cagaaaatca ccgatgcgta tgccgaaaat
    14161 ccaaaaatcg ctaacctgct gttggctccg tatttcaaga aaatcgccga cgactaccag
    14221 caggcactgc gtgatgtcgt cgcttatgca gtgcaaaacg gtattccggt tccaaccttc
    14281 tctgcggcgg ttgcttatta cgacagctat cgtgccgcgg tgctgcctgc gaacctgatc
    14341 caggcacaac gtgactattt cggtgcgcat acttataagc gcattgataa agaaggtgtg
    14401 ttccataccg aatggctgga ttaatctgat ttaatcagca ataatttgag gcccagcata
    14461 tattgctggg tttttttttg ccaataatct tatagattaa atttcattgc ttaatattag
    14521 cttaataaat atcagctact cttataaata aagtctgatt ttgttgttac gttgatgctg
    14581 ggatctgcgt agcgctaaaa actttaaccc ctatcaggat gctaaaacat catgattcac
    14641 agttaagtta attctgagag catgaaatga aaattaccat ttccggtacc ggctatgtgg
    14701 gcctgtcgaa tgggcttcta atcgcacaaa accatgaggt tgttgcatta gatattttgc
    14761 cttcacgtgt tgcaatgctg aatgatcgga tatctcctgt cgttgataaa gagattcaaa
    14821 attttttgca gtcagatgaa attcggttta acgccacgtt agataaaaaa gaagcctatc
    14881 ggaatgcaga ttacgtgatc atcgcaacgc ctacagatta cgatccaaaa acgaactatt
    14941 ttaatacgtt tagcgtggaa tctgtcattc aggatgtcat tgaaataaat ccacttgcag
    15001 ttatggttat caagtccact gtcccggttg gttttaccgc ggcaatgcag acgaaatatc
    15061 atactgagaa cattattttt tctccagaat tcctccgcga aggcaaagcc ctttacgata
    15121 accttcatcc atcccgcatt gttattggtg agcgttctga acgtgcagag cgttttgctg
    15181 cgttattaca ggaaggggcc attaagcaaa atatcccgac cctgtttacc gactccactg
    15241 aagcagaagc gattaaactg tttgccaaca cctatctggc aatgcgcgta gcgtacttta
    15301 acgaactgga tagctatgca gaaagtttag gtctcaatac gcgtcagatt atcgaaggtg
    15361 tttgccttga cccgcgtatt ggcaaccatt acaacaatcc gtcgtttggc tatggtggct
    15421 actgcttgcc gaaagatact aagcaactac tggcgaatta tcagtctgtg ccgaataacc
    15481 tgatctcggc aattgtcgat gctaaccgta cgcgtaaaga ctttattgcg gatgccattt
    15541 tgtcacgtaa gccgcaagtg gtggggatct atcgccttat tatgaagagt ggttcagata
    15601 acttccgggc gtcttctatt cagggaatta tgaagcgtat caaggcgaaa ggcgtagaag
    15661 taatcatcta tgaaccagta atgaaagaag actcattctt caactcgcgt ctggagcgcg
    15721 atctcgccac cttcaaacaa caagccgata tcattatctc caaccgtatg gcagaagagc
    15781 ttaaagatgt cgctgataag gtctacaccc gcgatctctt tggtagtgat taa
//
