LOCUS       KY574602               16183 bp    DNA     linear   BCT 19-SEP-2017
DEFINITION  Escherichia albertii strain ZG141049 O-antigen biosynthesis gene
            cluster, partial sequence.
ACCESSION   KY574602
VERSION     KY574602.1
KEYWORDS    .
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16183)
  AUTHORS   Naumenko,O.I., Zheng,H., Senchenkova,S.N., Wang,H., Li,Q.,
            Shashkov,A.S., Wang,J., Knirel,Y.A. and Xiong,Y.
  TITLE     Structures and gene clusters of the O-antigens of Escherichia
            albertii O3, O4, O6, and O7
  JOURNAL   Carbohydr. Res. 449, 17-22 (2017)
   PUBMED   28672166
REFERENCE   2  (bases 1 to 16183)
  AUTHORS   Zheng,H. and Xiong,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2017) State Key Laboratory for Infectious Disease
            Prevention and Control, National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, P.O.Box 5, Changping, Beijing 102206, P.R. China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SOAPdenovo v. 1.04
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16183
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="ZG141049"
                     /serotype="O7"
                     /db_xref="taxon:208962"
     misc_feature    <1..>16183
                     /note="O-antigen biosynthesis gene cluster"
     CDS             1..894
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase GalF"
                     /protein_id="ARO73574.1"
                     /translation="MANLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIV
                     AAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQ
                     GEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVL
                     AKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIW
                     PELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNL
                     KEGAKFRKGIEKLLSE"
     CDS             1251..1871
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="ARO73575.1"
                     /translation="MKKKLIIIGAGGFAKAVIDSLDHDEYVLEGFIDSLKSGMHQGYPI
                     VGHSLSDIRLPTDYYYFIAIGDPDDRALWLRQIRDLKLDTINVIDKTAIVSMRSSIGTC
                     IYIGKMAIVNCDSQLEDGVIINTRALIEHGNYISYCTNVSTNVVLNGDVYIGEKSFIGS
                     CSVVNGQIKIGRSTIVGSGSVVIRDIPDNVVVAGTPTRLIRER"
     CDS             1874..2914
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="ARO73576.1"
                     /translation="MSKVYIVAEIGCNHNGDYFLAKKMIKEAKAAGVDAVKFQTFKADQ
                     LISKYAPKAEYQKKVTGNEESQLEMTRRLELPYEEFIKLEEYAKELGLDVFSTPFDLES
                     IDFLASRNQKMWKIPSGEILNLPYLEKICRLPIKDKKIVLSTGMATLDEIRECLNVFNK
                     NGMSPENITILHCNTEYPTPFEDVNLNSIHGLKKEFKEYNIGFSDHSPGFFAGIAAVPY
                     GITFIEKHFTLDKNFEGPDHKASVTPEELRLLCQGIREVEKSLGSYIKLVTKSERKNKI
                     VARKSIVAKRNIKKGEVFTIDNITIKRPGNGISPMYWYELLGKKAERDFDEDQLIIQSG
                     FVAQEV"
     CDS             2917..4179
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate cytidylyltransferase"
                     /protein_id="ARO73577.1"
                     /translation="MVNRLAIIPARSGSKGLANKNILMLMGKPLIVYTIDAAISSKCFS
                     KVIVSTDSIEYKYIAEKYGAEVVMRDERLASDTATSFMVIEDILKKYRGFDYFVLLQPT
                     SPFRNAKHIQESIDLFESKKGINFLVSVTESNKNSELIKPLDESHRLTNFGGDFSNYRR
                     QNVKEYSPNGAIFIGRNDSYLKKKHFFGSDSVAYIMNKEDSVDIDDRVDFEIAISLATK
                     KMKQKLLIKQIKHRIAEKFNEINPSAPITLIGHSLFDYWGIDTLAGKKVNNYGIAGINS
                     EQYYQFVLKKRLISNLGDIVFLYSGTNDIVLENWTAKYTIYWTTKIIEELKKINSNVKI
                     YLLAVPPVNGRVDRNNYIINELNHLLFHHVKCLNNVYWVPLSESFYDEFGNLKHSFTSD
                     GLHFTKKAYEQLERDLEEVMK"
     CDS             4176..5357
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-glucosamine 2-epimerase"
                     /protein_id="ARO73578.1"
                     /translation="MKNILYVTGSRAEYGIMKRLLLQLDKEDDIKLTIIATGMHCDEKY
                     GLTYKFIENDGLKIEKLINIDIDNSSNAGILKTMAKCQTEFGDYFESNKFDAIIILGDR
                     YEILSVAIAAAIHNLPIIHLHGGEQTLGNYDEFIRHAITKMSNLHLVSTDEYRDRVIQM
                     GEHPDLVHNIGALGAENVIKMTLPTIMELENKFGSLARPYFMVVFHPETLNEVSPITQI
                     EELLGALDLLKACFDFIFIGSNSDTKADEIAIRLKKYCQQNKNRFIPSTEPEEYIALCK
                     YSQGLIGNSSSGLIEIPSLKIPTINIGNRQKGRVRGNSVIDTDCKTYDIYHSILLSQTH
                     DFKNKTSLSGNPYYRDNALSHALSIIKSFIINNKKDVKKFYDIKINADNESNV"
     CDS             5354..6622
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ARO73579.1"
                     /translation="MSIYKNSSIYLISNIFNALIPFMLLPVLTRNLTPYEYGQIAMFQI
                     LVSGLSSLTGLNTVGAANRRYYDVNSKNELAAYNTNCFYILILSCTILLLLSLLLSDLL
                     SSLLTIPKNWIYLSVAVSGATFIIQFRLGQWQIRERAFWFGVLQISQGVVVSVLTIIFL
                     IYMCQGASSRVNSLFIVSIVYALISLYSLHKSKLIIFTGINFSNIRDALYFGVPLIPHV
                     LGIFCLSALDRVLINGVLGVSEAGIYMLAAQLSLGMTVFFDALNKALVPWLFNVLSLND
                     VNKINSLIRFTYLFFIVVAFLGGLSFWVGPFVVELVAGKAYLKAKDIIGWLCLGQAFNG
                     MYLMVTNYLFYAKCTGKLSIVTISSGGANIVLLLILMKFSGITGVAIAFSISMFIRFLA
                     TWWLATKVCGFSWRMPIRRIIWG"
     CDS             6667..7605
                     /codon_start=1
                     /transl_table=11
                     /product="GT52"
                     /protein_id="ARO73580.1"
                     /translation="MNLFICRTIFQAYYARVLIKAKSIKDVMFVYLSEELSDREKNVLS
                     CSGIDYIVIGGSHSYKRLLKQIWLLNYLYRVTKKQSVDFYFSSIDDPFIQTIISRNKFS
                     NIYTFDDGTANYVPNSFFYIEKKKKLSVRIKYYLMGNRIRNLHGVKKLIKTHYSTNHFK
                     NIIDDVEYIPLHSKKNFSDLPVESKKHEVVNIYLCPNFDEVYIDAENVIKKFISTLTKH
                     DIIIPHPRDKLIWGAGENYKIRNDTMAEIVIDEYLSKGCLINLFGIANSTQYHYLQNNK
                     IINHVIDIGKIKPEFEEAILKQIECFKNLTN"
                     /old_product="glucosyltransferase"
     CDS             8045..8680
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ARO73581.1"
                     /translation="MKQYGDFENDIPVHDAIARVVSCISPAKFHECFINWMHDCHSSDD
                     KDVIAINGKTLRHSYDKSRRRGTIHVISVFSTMLSLVIGQIKTDEKSNEITTIHKLLNM
                     LDIKGKIIITDAMGCLKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEH
                     DSYAISEKATVETKSVFILFVMSLMKLSISRLNGKDRRNYAWQSPFGL"
     CDS             9061..10164
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ARO73582.1"
                     /translation="MNFLLFFSICYFLYVTSIGMLFNNYKVLLIFSLIPPTIVSVIRYN
                     VGIDFMSYVDYFNQLKYTDEIYLDDTFKYISYFSYYIGGNEQIVFLIYAIFYSAALYFL
                     IKLALENYITRINNFYVVGLLLSFYSFYFLLSFNQIRSSLSALLLCYGLLKRKKDFSFV
                     MAILLSVLFHSAAMFILPFYVALRKIKVRYLLVIFPFLLVASFYNIFSDVLKFILTFLN
                     SRFLTYFYSEYFVPKTGMEKMYSVISMTIVLGMVVCLFKLLPEKFDLMIKFVILFVLLR
                     AMSIDILIFARISDFLKPITIILVFTAIYFASNKVTPRIILPIYLIMILLLCLFNIKIG
                     SNITKDDHYTYGYNICLFGNKCVEEFY"
     CDS             10178..11242
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="ARO73583.1"
                     /translation="MLLVSMEKEMRNFSCKDPLSLVENQIVLSIILPVYNVSEYLIECL
                     NSLLLDVHECYLNRCEVIVVDDGSTDNSSELMREYSLQYPEIVKIYSKSNGGLSDARNY
                     GLLKSSGKYISFVDSDDVVNRDFIKKIINIIDMYDFDILSFDFIRFFNKRPPPVVSEIG
                     YITQMVERVDPDFYKSKPVFAWNKVYRRYLFDNEIFPKGWYYEDVALIPLLLDRAKRLY
                     HINSVCYFYRQRQGAITFFNDNKYLDILKGVSFLYGRSQSTFIKTVIINQFFTLTLLSL
                     RLPTSNYFMNMRYIVDTYCEKFELDSFEPELILKHIPFLLLKKLKSLCVYPLYLFKPAV
                     YFHKQLKHFRGRLK"
                     /old_product="glycosyltransferase"
     CDS             11239..12348
                     /codon_start=1
                     /transl_table=11
                     /product="STP|Pyr_redox"
                     /protein_id="ARO73584.1"
                     /translation="MKYDYIIVGSGLFGSVFAHELGKINKKIKVIERREHVGGNVYCEN
                     IEGINVHKYGAHIFHTNDKEIWDYVNQFVEFNQYINSPLAFSKGKIFNLPFNMNTFNQL
                     WETITPQQAKEIIKKQSSEISGNKPRNLEEQAISLVGKDIYNCLIKEYTEKQWGRPCTE
                     LPPFIIKRLPVRFTYDNNYFNDRYQGIPVGGYNKLIDALLNGIEVETCTDYFEKKKYWD
                     SVAKKIIYTGPIDKFFDYKFGALDYRSLRFEHELLDVDNFQGNAVVNYIDKKYLFTRII
                     EHKFFEFGDQCNTVITREYPKEFEEGDEYYYPINDEKNMQTYKMYYNLSKQKKNVIFGG
                     RLAEYKYYDMHQVIKSALNAFRKEVGSGE"
     CDS             12341..13255
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="ARO73585.1"
                     /translation="MNKEVIAIIVTYNRKELLLKVIDAVLNQSYPLKKILIIDNNSTDG
                     TEMFISSRLSELVVYENTGDNLGGAGGFYRGFIEAEKYGYDYLWLMDDDLMPTVNCLEI
                     LISNNIDGIVQPVRYNLDESCAELSPLTYDLSNPFKLNPKGTPLKNYLDTIGNKSGKVD
                     IEAIPFEGPLISRKVVEKIGYPEPKFFIFCDDIEYAIKAKRNGIQIQCDLQAKAYRLLV
                     NNQANDLLSWKGYYMLRNVFYLHKTYGTNFFVRQKPIVLGLGYALLCVLKCNFSQLPVI
                     WRAFWDSSSLRNTEKFRPKMRKK"
     CDS             13369..14775
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="ARO73586.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIENRGYTVSIFNRSREKTEEVIAE
                     NPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLEKGDIIIDGGNTF
                     FQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVA
                     EDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQTFTEW
                     NNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLIT
                     ESVFARYISSLKEQRVAASKVLSGPQAQPVGDKAEFIEKVRRALYLGKIVSYAQGFSQL
                     RAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPKIANLLLAPYFKKIADD
                     YQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDK
                     EGVFHTEWLD"
     CDS             15017..16183
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="ARO73587.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISPV
                     VDKEIQNFLQSDEIRFNATLDKKEAYRNADYVIIATPTDYDPKTNYFNTSSVESVIQDV
                     IEINPFAVMVIKSTVPVGFTAAMQTKYHTENIIFSPEFLREGKALYDNLHPSRIVIGER
                     SERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLG
                     LNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANR
                     TRKDFIADAILSRKPQVVGIYRLLMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMK
                     EESFFNSRLESDLVTFKQQADIIISNRMAEELKDVADKVYTRDLFGSD"
ORIGIN
        1 atggcgaatt taaaagcagt tataccggta gcgggacttg gaatgcatat gttgcctgcc
       61 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca aacccatgat tcagtatatt
      121 gttgacgaaa ttgtggctgc tgggatcaaa gaaatcctcc tggtgactca tgcgtccaag
      181 aacgcagtcg aaaaccactt cgatacttct tatgaattag aatcgctcct tgagcagcgt
      241 gttaaacgtc agttgttggc agaagtacag tctatctgcc cgccgggcgt aaccattatg
      301 aacgtgcgtc agggtgaacc tttaggtttg ggtcactcca ttctgtgcgc cagacccgcc
      361 attggcgaca acccgtttgt cgtggtgctg cctgatgttg ttattgatga cgccagcgca
      421 gatccgctgc gttacaacct tgccgccatg attgcgcgat tcaacgaaac gggtcgcagt
      481 caggtcttgg caaaacgtat gccgggtgac ctttctgaat actccgtcat ccagactaaa
      541 gagccgctgg atcgtgaagg caaagtcagt cgcattgttg aatttatcga aaaacccgat
      601 cagccgcaga ctttggattc agacatcatg gcggttggtc gttatgtgct ttctgcagac
      661 atttggcctg aactggaacg gactcaaccc ggtgcgtggg ggcgtattca gctaactgac
      721 gcaattgctg aactggcgaa aaaacagtcc gttgacgcaa tgttaatgac gggtgacagc
      781 tacgactgcg gtaaaaaaat gggctacatg caggcatttg tgaagtacgg actacgcaac
      841 ctgaaagaag gagcgaagtt ccgcaaaggg attgagaagc tgttaagcga ataaagaact
      901 gaccgaatgt aacggttgat aagaaaatta taacgacagt gaagattcgc ggtgagaatg
      961 aatcgcaacg aagactcttg tcgttgttgt tttataaaac accataataa taatgagtta
     1021 ttaataggct tttagttaaa ttttccagga ttttccttgt ttccagaacg aattggtaag
     1081 acaattagaa tagtatcttt tcattatatc aagtaattga gaaggttttt tgattctaaa
     1141 atatattcct gcagtacgct ggtagctgtt aagccagggg cggtagcgtg tcttatggat
     1201 ccttctgtgg aagataaatt caaatactga accacactta tggtgcgtaa atgaagaaaa
     1261 aattaatcat tatcggagct ggtgggtttg ctaaagcggt aatagatagt ttggatcatg
     1321 atgagtacgt tcttgaagga tttattgact ctcttaaatc aggaatgcat caaggatatc
     1381 cgatagttgg tcattcatta agtgatattc gtttgccaac tgattattat tattttattg
     1441 ccattggtga tcctgacgat agagcattgt ggttaagaca aatacgagat ctaaagcttg
     1501 acaccataaa tgtcattgat aaaacggcaa ttgtttcaat gagatcatct atcggaacat
     1561 gtatttacat tggtaaaatg gctatagtaa attgcgattc acaattagaa gatggcgtta
     1621 ttattaatac tagggctttg atagagcatg ggaattatat ttcatattgt acaaatgttt
     1681 caactaatgt agtcctcaat ggtgatgtat atatcgggga gaaaagtttt ataggtagtt
     1741 gtagtgtagt gaatggtcaa ataaaaatag gcaggtcaac tattgtaggc tcagggtcag
     1801 tagttattcg tgatattcct gataatgtgg ttgttgcagg cacaccaaca agattaataa
     1861 gagaaaggtg aaaatgagta aagtatatat tgtggcggag attggttgta atcataatgg
     1921 tgattatttt cttgccaaga aaatgattaa agaggcaaaa gccgcaggtg ttgatgcagt
     1981 taaattccag acattcaaag cggatcaatt aatttcgaag tatgcgccta aagctgaata
     2041 tcagaaaaaa gttacaggga atgaggaatc ccagttagaa atgacaagaa ggcttgaatt
     2101 accttatgaa gagtttataa agcttgaaga gtatgcaaaa gagttaggtc ttgatgtctt
     2161 ttcaacccca tttgatcttg agtcgattga ttttttggca tcgcggaacc aaaaaatgtg
     2221 gaaaattcct tcaggagaaa tccttaattt accgtatcta gaaaagattt gtcgattacc
     2281 aataaaggat aaaaaaattg ttctttcgac aggaatggcg acgcttgatg aaataagaga
     2341 gtgtttaaat gtttttaata aaaatggaat gtctccagaa aatataacga tattacattg
     2401 caatactgaa tatcctacac catttgagga tgtgaatcta aactcaattc atgggttaaa
     2461 gaaagagttt aaagaatata atataggatt ctcagatcat tctcctggat ttttcgctgg
     2521 aatagcggca gtgccctatg ggattacttt tattgaaaaa cattttactc tggataaaaa
     2581 tttcgaaggg ccagatcata aagcatctgt aactcctgaa gaattacgtc tcctgtgtca
     2641 ggggattcgt gaagttgaga aatctcttgg tagttatatt aaattagtta ctaaatctga
     2701 gcggaagaat aaaattgtag cgagaaaatc tatcgtagcc aagcgtaata taaaaaaagg
     2761 agaagttttt acaattgata atattacgat aaagcgacct gggaatggaa taagtcctat
     2821 gtattggtat gagctattgg gtaaaaaagc agagagggat ttcgatgaag atcaactgat
     2881 cattcagtct ggttttgttg ctcaagaggt ataataatgg taaatagatt ggctattata
     2941 ccagcacgtt ctggttcgaa agggttagct aataaaaata tactgatgtt gatggggaaa
     3001 ccattgattg tttatacaat agatgctgcc ataagttcta aatgtttctc taaggtaatt
     3061 gtttcaacag attctataga atataaatat attgccgaga agtatggcgc tgaagttgtg
     3121 atgcgtgatg agagacttgc aagtgatact gcaacatctt ttatggtaat agaagatatt
     3181 ttaaaaaaat atagaggttt tgattatttc gttttgttgc aacctacgtc accatttcga
     3241 aatgccaaac atatacaaga gtctattgat ttatttgagt ccaagaaagg aataaatttt
     3301 ttagtatcgg taacagagag taataagaat tcagaattaa ttaagccatt agatgagtca
     3361 catagattaa caaattttgg aggtgatttc agtaactatc gacgacaaaa tgttaaagaa
     3421 tattcaccta atggagcgat ctttattggt cgcaatgata gttatttaaa gaaaaaacat
     3481 ttttttggtt ctgacagtgt tgcttatata atgaataagg aagattctgt tgatattgat
     3541 gatcgagttg attttgaaat agcgatttcg ttagcaacca aaaaaatgaa gcagaaattg
     3601 ctaataaaac aaattaaaca tcgcattgct gaaaagttta atgagataaa tcctagtgca
     3661 cctattacat taatcggtca ttctctattt gattattggg gaattgatac tttagcaggg
     3721 aaaaaagtta ataattatgg tatcgctgga ataaatagcg agcaatatta tcagtttgtt
     3781 ttaaaaaaaa ggcttatctc aaatctcgga gatattgttt ttttatactc aggtacaaat
     3841 gatatcgtgc ttgagaattg gactgcaaag tacactattt attggacaac caaaataatt
     3901 gaggagttaa agaagataaa ttcgaatgtt aagatttatc tcttagctgt gcctcccgta
     3961 aatggaaggg ttgatcgcaa caattatatt attaatgagt tgaatcattt gttatttcat
     4021 catgttaaat gcttaaataa cgtatattgg gttccgcttt cagagtcatt ttatgatgaa
     4081 tttggtaacc ttaaacattc atttactagt gatggattgc attttacaaa aaaagcttat
     4141 gagcaacttg agcgagattt ggaagaggta atgaaatgaa gaatatatta tatgttacag
     4201 gctcacgtgc tgaatacggt ataatgaaga gattattact acaattagat aaagaagatg
     4261 atattaaact aacaattatt gctacgggaa tgcattgtga tgaaaaatat ggattgacgt
     4321 ataaatttat tgaaaatgat ggtttaaaga tagaaaagtt aattaatatt gatatagata
     4381 atagttctaa tgctggtatt cttaaaacaa tggcaaaatg tcaaacagaa tttggtgatt
     4441 actttgaaag taataagttt gatgctataa ttatattagg tgatcgatat gagatattat
     4501 cagtagctat tgccgccgca attcataatt taccaattat acatttgcat ggaggtgagc
     4561 aaacattagg aaattacgat gaatttatac gccatgctat aacaaaaatg agtaatcttc
     4621 atctggtatc aaccgatgaa taccgtgata gggttatcca aatgggagaa catcctgact
     4681 tggtccataa tataggagcc cttggagctg aaaacgttat taagatgaca ttgccaacta
     4741 taatggagct cgaaaataaa ttcggttctc ttgctcgtcc ctattttatg gtggtttttc
     4801 atcctgaaac attaaatgaa gtatcaccaa ttacacaaat agaggaacta cttggagcat
     4861 tggatttatt aaaagcttgc tttgatttca tttttattgg ttcaaactct gatacaaaag
     4921 ccgacgagat tgctattagg ttaaaaaagt attgccaaca gaataagaat cggtttattc
     4981 catcaactga acctgaagaa tatattgcat tatgtaaata ttctcaaggt ttaattggaa
     5041 attcgtcatc tggactaata gagataccat ctttgaaaat cccgactata aatataggca
     5101 atagacaaaa agggcgagtc cgtggaaatt ctgtaattga tactgattgt aagacatatg
     5161 atatctatca ttccatactt ttaagccaaa cgcatgattt taagaataaa acttctttgt
     5221 ctggaaatcc ttattatcgt gataacgctt taagtcatgc cttaagtata attaagagtt
     5281 ttataataaa taataagaaa gatgtaaaaa aattctatga tataaagata aatgcggata
     5341 atgagtcaaa cgtatgagta tatataaaaa ctcaagtatt tacttgatat caaatatatt
     5401 caatgcatta attccattta tgttgcttcc tgtgctgaca agaaatctaa caccttatga
     5461 atatgggcaa atagccatgt tccaaatatt agtaagtgga ttgtcttctc taacaggttt
     5521 aaatacagtt ggtgctgcaa atcggcgcta ctatgatgta aattcaaaaa atgaactggc
     5581 tgcttacaat acaaactgtt tttatatatt aattttaagt tgtacgatat tacttctttt
     5641 atctctattg ttatctgacc tattgagttc attgttgaca attccaaaaa actggatata
     5701 tctatcagtt gcggtttctg gggcgacatt tataattcaa tttcgtttgg gacaatggca
     5761 gatccgtgag agagcttttt ggtttggtgt attacagatc agccaagggg tcgttgtatc
     5821 cgtgttaact attatttttt taatttacat gtgtcaggga gcatctagca gagtaaattc
     5881 attatttata gtaagtatag tatatgcact gattagtctc tatagtttac ataagagtaa
     5941 attaatcata tttacgggaa ttaatttttc gaatattaga gatgctctat attttggagt
     6001 tcctttgata ccacatgtct taggaatttt ttgtctaagc gcattagata gagttttaat
     6061 taatggtgta ttaggtgtca gtgaggccgg aatatatatg ttggcggctc agttaagttt
     6121 gggaatgacg gttttttttg atgcgttaaa taaagcatta gtgccttggt tgtttaatgt
     6181 tctgtcgtta aatgacgtaa ataaaataaa ttcactgatt aggtttacat atctattttt
     6241 tattgttgtt gcttttttgg gggggctttc tttttgggtt gggcctttcg ttgttgagtt
     6301 agttgccgga aaggcgtatc tgaaagcaaa agatattatt ggctggcttt gcttaggaca
     6361 ggcttttaat ggtatgtacc ttatggttac aaattattta ttttatgcaa agtgtacggg
     6421 gaaattatca attgtaacca ttagcagtgg gggcgcaaat atagttctgt tattgatatt
     6481 gatgaagttt tcaggaataa ctggagtagc tatcgcattc tcaatatcaa tgtttattag
     6541 atttttagct acgtggtggt tagccacgaa agtgtgtggg ttttcatggc ggatgccaat
     6601 taggagaatt atttggggat gaggtttttt gcataagtaa atatataata aggataaggg
     6661 ggggacatga atttatttat atgccgaacc atatttcagg cgtattatgc aagagtgttg
     6721 attaaagcca aaagtattaa agatgttatg tttgtttatt tatctgaaga gttgtctgat
     6781 agagaaaaaa atgtactatc gtgttctggt attgactata tagttattgg aggttcgcat
     6841 tcgtataaac gactcttaaa acagatttgg ttgttgaatt atttgtatag agtgaccaaa
     6901 aaacaatctg tagactttta tttttcaagt attgatgatc catttattca aacaattatt
     6961 tcaaggaata agtttagtaa catatatacg tttgatgatg gaacggctaa ttacgtacca
     7021 aattcctttt tctacatcga aaagaaaaaa aagctatctg taaggattaa atattatctt
     7081 atgggaaata gaataaggaa tttacatggg gttaaaaaac taattaaaac acactattca
     7141 actaatcatt ttaaaaatat catcgatgat gtggagtata ttcctttaca tagtaaaaaa
     7201 aacttttctg atttgcctgt cgagtcaaaa aaacatgaag tggttaatat atatctatgt
     7261 cctaattttg atgaggtcta catcgatgct gaaaatgtaa taaagaaatt tatatctaca
     7321 ctgactaaac atgatattat aatacctcat ccaagagata aactcatatg gggtgctggt
     7381 gagaattata aaataagaaa tgatacgatg gcggaaatag taatagatga atatctgtct
     7441 aagggatgtt tgataaattt atttggaatt gccaattcaa cgcaatatca ttatctccaa
     7501 aataataaaa ttataaatca tgtgattgat attggtaaaa taaagccgga attcgaagag
     7561 gcgattctaa agcaaattga gtgttttaaa aatctgacaa attaaaatga ttgggtcgtg
     7621 tattcaagat attgattact gttattgtgg ataatactcc cgctcaataa atggaccaac
     7681 actttgtgca tttcgaatac cctagagtct ttcgaactag gtgtttcgcc aaggaaagag
     7741 cacgtaacgc tgcttttttg tactaaataa ttcgcactaa atgttcaatg attgagatat
     7801 ttcttattac ctagagctgt ttttcattgc ttatacatga tcaaatactc cttacataat
     7861 taaggaggac aaaatggaac ttaaaaaatt gatggaacat atttctgtta ttccagatta
     7921 cagacaagtc tggaaagtgg aacataaact gtcagatatt ctactgttga ctatttgagc
     7981 cgttatttct ggtgccgaag gttgggaaga tatagaggat ttcggggaaa cacatcccga
     8041 ttttttgaag caatatggtg attttgaaaa tgatattcct gttcacgatg ctattgccag
     8101 agttgtatcc tgtatcagtc ctgcaaaatt tcatgagtgc tttattaact ggatgcatga
     8161 ttgtcattct tcagatgata aagacgttat tgcaattaat ggaaaaacgc tccggcactc
     8221 ttatgacaag agtcgccgca ggggaacgat tcatgtcatt agtgtgttct caacaatgct
     8281 cagtctggtt atcggacaga ttaagacgga tgagaaatct aatgagatta caactatcca
     8341 taaacttctt aacatgctgg atattaaagg gaaaattatc ataactgatg cgatgggttg
     8401 cctgaaagat attgctgaga agatacaaaa acagggaggt gattatttat tcgctgtaaa
     8461 aggaaatcag gggcggctta ataaagcctt tgaggaaaaa tttccactga aagaattaaa
     8521 taatccagag catgacagtt acgcaattag tgaaaaagcc acggtagaga cgaaatccgt
     8581 cttcatatta tttgtgatgt ccctgatgaa attatcgatt tcacgtttga atggaaagga
     8641 ccgaagaaat tatgcgtggc agtctccttt cggtctataa tagcagacaa aagaaagaac
     8701 ccgaataatg gtcagatgtt atatcagttc tgctgattta actgtagaga agttcgccac
     8761 agcgatccga aatcactggc acgtggagaa taagctgcac tggcgtctgg atgtggtaat
     8821 gaatgaagac gactgcaaaa taagaagagg aaacgcagca gaattatttt cagggatacg
     8881 gcacaccgct attaatattt tgacgaataa taatgtattc aagacagggt taagacgtaa
     8941 gatgcgaaaa actgtccatg gacagaaact atctggcatc agtccttacg agaagcgggc
     9001 tttcgtaatc ttgccctgtt gagataggct cttgatgaat aaaggtttga tggtaattaa
     9061 atgaactttc ttttgttttt ttcaatatgt tattttttgt acgtaacctc aatagggatg
     9121 ttatttaata actataaagt ccttttgatt ttttcgctaa tccctccgac aatagtgtca
     9181 gttataagat ataacgtcgg cattgatttt atgtcatatg ttgattattt caatcagctt
     9241 aaatatacag atgagatata tttagacgat acatttaagt atataagtta tttttcttat
     9301 tatataggtg ggaatgaaca gattgtattt ttaatatatg ctatttttta ctctgcagca
     9361 ctttattttt taataaaatt ggcgttagag aattatataa caagaataaa taatttttat
     9421 gtggttgggt tgctattgtc attttattcg ttttatttct tattgtcatt taatcaaatt
     9481 agatcttcat tatcggccct attgctatgt tatgggttgc taaaaagaaa aaaggatttt
     9541 tcttttgtaa tggcgatttt gttaagtgtt ctttttcatt ctgcagccat gtttatattg
     9601 cctttttatg ttgctctaag aaaaataaag gttagatacc tattggttat attccctttt
     9661 cttcttgttg cttcatttta taacattttt tcagatgttt tgaagtttat tcttacattt
     9721 ctaaatagtc gttttttgac atatttttat tctgaatatt ttgttccaaa gacgggaatg
     9781 gaaaaaatgt atagtgtcat ctcaatgact attgtcttgg gaatggttgt ttgtttgttt
     9841 aaattgttac ccgaaaaatt tgatttaatg ataaagtttg ttatactttt cgtcctactt
     9901 cgagcgatgt caattgatat attgattttt gccagaatca gtgattttct taaaccaata
     9961 actataattc tggtatttac tgctatatac tttgcttcaa ataaagtaac acctagaatt
    10021 atccttccta tttatttaat tatgatattg ctcctctgtc tttttaatat aaagattgga
    10081 tcgaatataa ctaaggatga ccattatact tatggctata atatttgcct ttttggtaat
    10141 aaatgtgtag aggagtttta ttaacaatta ttattttatg cttctggtat ctatggaaaa
    10201 agaaatgaga aacttttctt gtaaagaccc cctttcactt gttgaaaatc aaattgtatt
    10261 gtcaattatt cttcctgtat ataacgtatc agaatattta attgagtgcc taaatagttt
    10321 gctgttagac gtccatgagt gttatcttaa tagatgtgaa gttattgttg ttgatgatgg
    10381 ttcaacggat aattcctctg aattaatgag agagtattca ttacaatacc ctgaaatcgt
    10441 taagatttac tcgaaatcta atggtgggct ctcagacgca cgtaactatg ggttattgaa
    10501 atcctccggt aaatacattt cctttgttga ttcagacgat gttgttaata gggattttat
    10561 aaaaaaaata atcaatatca ttgatatgta tgattttgac atattgtcat ttgattttat
    10621 aaggtttttt aataaacgac ccccccctgt tgtttccgaa ataggctata taacccaaat
    10681 ggttgaaaga gtagatcccg atttctataa atctaagccc gtttttgctt ggaataaagt
    10741 ttatcgacgt tacctgtttg acaatgaaat atttcctaag ggatggtatt atgaagatgt
    10801 tgcattaatc ccgttattat tagatagagc aaaacgttta taccatatta acagcgtttg
    10861 ttatttttat cgacagagac aaggagcaat aacttttttt aatgataata aatatcttga
    10921 tattttaaaa ggagtatctt ttttatatgg caggtcacaa tccacattta ttaaaactgt
    10981 gattataaat cagttcttta cgttaacttt gttatcttta agactaccaa ctagtaatta
    11041 ttttatgaat atgaggtata ttgttgatac ctattgtgaa aaatttgaat tggactcttt
    11101 tgaacctgag ctgatattaa aacatattcc ttttttatta ttaaaaaaat tgaagtcact
    11161 atgtgtttat ccgttgtatt tatttaaacc agcggtttat tttcataaac aacttaaaca
    11221 ttttagaggg agattaaagt gaagtatgat tatataattg ttggtagcgg tctttttgga
    11281 agtgtttttg cacatgaatt aggaaaaata aacaaaaaga tcaaggttat tgagcgtcgt
    11341 gaacatgttg gtggtaatgt atattgtgag aatattgaag ggatcaatgt tcataaatat
    11401 ggcgctcata tattccacac taacgataaa gaaatatggg attatgtaaa tcaatttgta
    11461 gagtttaacc aatacataaa ttcaccatta gctttttcta aaggtaaaat ttttaattta
    11521 ccttttaaca tgaatacctt taatcaactc tgggagacaa taactcctca acaggccaaa
    11581 gagattataa aaaaacaaag cagtgaaata agtggaaaca aaccaagaaa tctcgaagaa
    11641 caagcaattt ctttagtggg aaaagatatt tataattgtt tgattaagga atacactgag
    11701 aaacaatggg ggcgaccttg cactgaattg ccaccattta ttataaagcg gctccctgtt
    11761 aggtttacat acgacaataa ttattttaat gaccggtatc aaggaatacc agtcggcggt
    11821 tataataaat taatcgatgc cttattaaat ggaattgaag tcgaaacatg cactgactat
    11881 tttgagaaaa agaaatattg ggattctgtt gcaaaaaaaa ttatttatac agggcctatt
    11941 gataagtttt ttgactacaa atttggggct ttggattatc gttcattaag atttgaacat
    12001 gaattactgg atgttgataa ttttcaaggg aatgctgtcg taaattatat cgataaaaaa
    12061 tacttattca ctcgcattat agaacataaa ttttttgaat ttggtgacca gtgtaataca
    12121 gtcattactc gagagtaccc aaaagaattc gaagaaggag atgaatacta ttatcctata
    12181 aatgatgaaa aaaacatgca aacctataaa atgtattata atctttctaa acagaaaaaa
    12241 aatgtgattt ttggtggacg tcttgcggaa tataaatatt atgacatgca tcaagttatc
    12301 aaatcagctt taaatgcatt ccgaaaagag gtaggttcag gtgaataaag aggtaatcgc
    12361 aataattgta acctataata gaaaagaatt attgctgaaa gttatagatg ctgttttgaa
    12421 ccaaagttat ccactaaaaa aaatattgat aatagataat aatagtacag atggtacgga
    12481 gatgtttatt tcgagcagac tctctgaatt ggtggtgtat gaaaatacag gggataattt
    12541 aggtggggct ggaggatttt atcgagggtt catagaggcg gaaaaatatg gttatgatta
    12601 tctttggtta atggatgatg atttgatgcc tacagtgaat tgtcttgaaa tattaatatc
    12661 aaataatata gatggtattg tccaacctgt tagatataac ttagatgaat cttgtgcaga
    12721 actttcgccg ttaacttatg atttatccaa tccatttaag ttaaatccta aaggaacacc
    12781 attgaaaaat tacttagata caattggtaa taagtcaggg aaagtagaca ttgaagctat
    12841 tccatttgaa ggtccattga tttcgaggaa ggttgttgaa aaaataggct atccagagcc
    12901 taagttcttt attttttgtg atgatattga atacgcgatt aaagctaaaa ggaatggaat
    12961 tcagatccag tgcgatcttc aagccaaagc ataccgcctt ttggttaaca atcaagccaa
    13021 tgatcttctt tcctggaaag gatattatat gcttagaaat gttttctatc tccataaaac
    13081 atatggaacc aatttctttg ttaggcaaaa accgattgtt ttgggattag gatatgcatt
    13141 gttgtgtgta ttaaaatgca atttctctca actccccgtt atatggcgtg ctttttggga
    13201 ttcatcttca ttaagaaata ctgaaaaatt caggccaaaa atgagaaaga aataatttgt
    13261 ttctgtgctc tttgggtttt taatgtcaat tttctaaatc actaaatcac taaatcacta
    13321 aatcactaaa tcacttaaca cgatatttat ccctgacagg agtaaacaat gtcaaagcaa
    13381 cagatcggcg tcgtcggtat ggcagtgatg gggcgcaacc ttgccctcaa catcgaaaac
    13441 cgtggttata ccgtctctat tttcaaccgt tcccgtgaaa agacggagga agtgattgcc
    13501 gaaaatccag gcaagaaact ggttccttac tatacggtga aagagtttgt tgaatctctt
    13561 gaaacgcctc gtcgcatcct gttaatggtg aaagcaggtg caggcacgga tgctgctatt
    13621 gattccctta agccatacct tgaaaaaggc gacatcatca ttgatggcgg taacaccttc
    13681 ttccaggaca ccattcgtcg taaccgtgag ctttctgctg aaggttttaa cttcattggt
    13741 acgggggttt ccggtggtga agagggcgcg ctgaaaggtc cttctatcat gcctggcggt
    13801 caaaaagaag cctatgaact ggttgcgcct atcctgacca aaattgctgc cgtagctgaa
    13861 gatggcgaac cgtgcgttac ctatattggt gcagacggcg caggtcacta tgtgaagatg
    13921 gttcacaacg gtattgaata cggtgatatg cagttgattg ctgaagccta ttcactgctg
    13981 aaaggggggc tgaatctctc caacgaagag ctggcacaga cctttaccga gtggaataac
    14041 ggtgagctta gcagctacct gattgatatc accaaagata tcttcaccaa aaaagatgaa
    14101 gacggtaact acctggttga tgtgatcctc gatgaagcgg ccaacaaagg cactggtaaa
    14161 tggaccagcc agagcgcgct ggatctcggt gagccactgt cgttgattac cgagtctgtg
    14221 tttgctcgtt atatctcttc tctgaaagag cagcgtgttg ccgcgtctaa agttctgtct
    14281 ggtcctcagg cacagccagt aggcgacaaa gcggagttca tcgagaaagt tcgccgtgcg
    14341 ctgtatcttg gcaaaatcgt ttcttatgct cagggcttct ctcaactgcg tgcagcgtct
    14401 gaagagtaca actgggatct gaactacggt gaaatcgcga agattttccg tgctggttgc
    14461 attattcgtg cgcagttctt gcagaaaatc accgatgcct atgccgaaaa tccaaaaatc
    14521 gctaacctgc tgttagctcc gtatttcaag aaaatcgccg atgactacca gcaggcactg
    14581 cgtgatgtcg tcgcttatgc agtgcaaaac ggtatcccag ttccaacctt ctctgcggcg
    14641 gttgcttatt acgacagcta tcgtgccgct gtcctgcctg cgaacctgat ccaggcacag
    14701 cgtgactatt ttggtgcgca tacttataag cgtattgata aagaaggtgt gttccatacc
    14761 gaatggctgg attaatctaa tttaatcagc aataaattga ggcccggcat atattgccgg
    14821 gttttttttg ccaataattt tacagattaa atttcattgc ttaatattaa cttaataaat
    14881 atcaactatt cttataagta aagtctgatt ttgttgttac gttgatgctg ggagcagcgt
    14941 agcgctaaaa attttaactt ctatcaggat gctaaaacat catgattcac agttaagtta
    15001 attctgagag catgaaatga aaattaccat ttccggtacc ggctatgtgg gcctgtcgaa
    15061 tgggcttcta atcgcacaaa accatgaggt tgttgcatta gatattttgc cttcacgtgt
    15121 tgcaatgctg aatgatcgga tatctcctgt cgttgataaa gagattcaaa attttttgca
    15181 gtcagatgaa attcgattta acgccacgtt agataaaaaa gaagcctatc ggaatgcaga
    15241 ttacgtaatc atcgcaacgc ctacagacta cgatccaaaa acaaattatt tcaatacgtc
    15301 tagtgtggaa tctgtcattc aggatgttat tgaaataaac ccatttgcag ttatggttat
    15361 caagtccact gtcccggttg gttttaccgc ggcaatgcag acgaaatacc atactgaaaa
    15421 cattattttt tcccccgaat ttctccgcga aggcaaagca ctttacgata accttcatcc
    15481 atcccgcatt gttattggtg agcgttctga acgtgcagag cgttttgctg cactattaca
    15541 ggaaggggcc attaagcaga atattccgac cttgttcacc gactcaaccg aagcagaagc
    15601 gattaaactg tttgccaata cctatctggc aatgcgtgta gcgtacttta acgaactgga
    15661 tagctatgca gaaagtttag gtctcaatac gcgtcagatt atcgaaggtg tttgccttga
    15721 cccgcgtatt ggcaaccatt acaacaatcc gtcatttggc tatggtggct actgcctgcc
    15781 gaaagatact aagcaactac tggcgaatta tcagtctgtg ccgaataacc tgatctcagc
    15841 aattgtcgat gctaaccgta cgcgtaaaga ctttattgca gatgccattt tgtcacgtaa
    15901 gccgcaagtg gtaggcattt atcgtttgct tatgaagagt ggttcagata atttccgtgc
    15961 gtcttctatt cagggaatta tgaagcgtat caaggcgaaa ggcgtagaag taatcatcta
    16021 tgaaccagta atgaaggaag agtcattttt caactcgcgt ctggagagcg atctcgtcac
    16081 cttcaaacaa caagccgata tcattatctc caaccgtatg gcagaagagc ttaaagatgt
    16141 cgctgataaa gtctacaccc gcgatctctt tggtagcgat taa
//
