LOCUS       CR626927               17455 bp    DNA     linear   BCT 06-FEB-2015
DEFINITION  Bacteroides fragilis NCTC 9343, complete genome.
ACCESSION   CR626927
VERSION     CR626927.1
DBLINK      BioProject: PRJNA46
            BioSample: SAMEA1705957
KEYWORDS    complete genome.
SOURCE      Bacteroides fragilis NCTC 9343
  ORGANISM  Bacteroides fragilis NCTC 9343
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 17455)
  AUTHORS   Cerdeno-Tarraga,A.M., Patrick,S., Crossman,L.C., Blakely,G.,
            Abratt,V., Lennard,N., Poxton,I., Duerden,B., Harris,B., Quail,M.A.,
            Barron,A., Clark,L., Corton,C., Doggett,J., Holden,M.T., Larke,N.,
            Line,A., Lord,A., Norbertczak,H., Ormond,D., Price,C.,
            Rabbinowitsch,E., Woodward,J., Barrell,B. and Parkhill,J.
  TITLE     Extensive DNA inversions in the B. fragilis genome control variable
            gene expression
  JOURNAL   Science 307 (5714), 1463-1465 (2005)
   PUBMED   15746427
REFERENCE   2  (bases 1 to 17455)
  AUTHORS   Cerdeno-Tarraga,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2004) Cerdeno-Tarraga A.M., submitted on behalf of
            the Pathogen Sequencing Unit, Sanger Institute, Wellcome Trust
            Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: amct@sanger.ac.uk
COMMENT     Accession number for plasmid pBF9343 in this genome project:
            CR626928.
FEATURES             Location/Qualifiers
     source          1..17455
                     /organism="Bacteroides fragilis NCTC 9343"
                     /mol_type="genomic DNA"
                     /culture_collection="ATCC:25285"
                     /culture_collection="NCTC:9343"
                     /type_material="type strain of Bacteroides fragilis"
                     /db_xref="taxon:272559"
     gene            complement(<1..1143)
                     /locus_tag="BF9343_1964"
     CDS             complement(<1..1143)
                     /locus_tag="BF9343_1964"
                     /old_locus_tag="BF2048"
                     /note="Similar to Staphylococcus aureus intercellular
                     adhesin molecule IcaA SWALL:Q9RQP9 (EMBL:AF086783) (412 aa)
                     fasta scores: E(): 1.3e-09, 22.3% id in 417 aa, and to
                     N-terminus of Bacteroides thetaiotaomicron two-component
                     system sensor histidine kinase/response regulator, hybrid
                     BT1183 SWALL:AAO76290 (EMBL:AE016930) (1336 aa) fasta
                     scores: E(): 8.7e-124, 77.83% id in 397 aa, and to entire
                     protein of Vibrio vulnificus glycosyltransferase VV12674
                     SWALL:Q8D9C9 (EMBL:AE016806) (409 aa) fasta scores: E():
                     1.1e-28, 28.97% id in 421 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glyco_tranf_2_3"
                     /protein_id="CAH07745.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1964"
                     /db_xref="EnsemblGenomes-Tr:CAH07745"
                     /db_xref="GOA:Q5LDR2"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q5LDR2"
                     /translation="MMTTLQILFWLSLFLVFYTHLGYGILLYMLVKLKELFVKPVRRQL
                     PPDKRLPDVTLFITAFNEEEVVDEKMRNCLGLDYPADKLHIVWTTDGSNDSTNQRLEDW
                     PQATVHFQPLRQGKTAAMTRGMMLVDTPLVVFTDANTMLNREAIREIVRAFEDPKVGCV
                     AGEKRIAVQEKDGAAAGGEGIYWKYESTLKALDARLYSAVGAAGELFAVRRELFTVMEP
                     DTLLDDFILSLRIAMQGYKIAYCTQAYAIESGSADIREEQKRKVRIAAGGLQSIWRLRE
                     LLNPFRYGVLTFQYVSHRVLRWSLAPVLLFALLPLNIAILLAGGSPVCYGTILALQLLF
                     YIMGGWGYYLSTRQVKNKLLFIPYYFLFMNINVMKGVNYLRKKKGTGAWEKAKRTKTES
                     LNQ"
     misc_feature    complement(order(1..69,97..165,169..237,1057..1125))
                     /locus_tag="BF9343_1964"
                     /note="4 probable transmembrane helices predicted for
                     BF2048 by TMHMM2.0 at aa 7-29, 303-325, 327-349 and
                     359-381"
     misc_feature    complement(490..981)
                     /locus_tag="BF9343_1964"
                     /note="Pfam match to entry PF00535 Glycos_transf_2,
                     Glycosyl transferase, score 50.1, E-value 4.1e-12"
     gene            complement(1152..2564)
                     /locus_tag="BF9343_1965"
     CDS             complement(1152..2564)
                     /locus_tag="BF9343_1965"
                     /old_locus_tag="BF2049"
                     /note="Similar to Bacteroides thetaiotaomicron hypothetical
                     protein BT1182 SWALL:AAO76289 (EMBL:AE016930) (500 aa)
                     fasta scores: E(): 2.3e-74, 46.18% id in 498 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="conserved hypothetical protein"
                     /protein_id="CAH07746.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1965"
                     /db_xref="EnsemblGenomes-Tr:CAH07746"
                     /db_xref="UniProtKB/TrEMBL:Q5LDR1"
                     /translation="MKIYIKTIAIFLLILLAASCIKEVDLYKGGSLREPAYLYPFDQEN
                     KNVTAEITIKTNSTINLELLDVYFAPLKYNKHLLIMLTQDDCKPSIYPRTWASINGKPL
                     SGQYYYHYEQLKQDDLPPDIYYLGKTLGCTDGTGKEVRFSFTATLAADDNYMAEESIIN
                     LGYSKDNYRFYGRNGILWEDVIDIVNYGNSIAFHNVNTKEIHNIDSIQKHYLIGQDSIQ
                     KRLSGRKCKTLSEPDGNIDYTTAAINLDDIKTITAERGEKVYPFHNLTNLENHTLNRVF
                     HDSPDDFKQVIEQERSIPTVDRCAINIGVHSTDAFWTDFLLWLNDTYGKDGEDCIWMPS
                     QEEYYEYNYYRMHGKIEKSADGSTLKLIINLPSQEYFYYPSVTINLKGLKKEDIKSIES
                     NSAVTGLSYGNYQDGVMLNIDCRRFLVEHATHFVEQYEKDKTNQSNKADALYFVNMLKE
                     SSKKAELLNRIK"
     misc_feature    complement(2499..2564)
                     /locus_tag="BF9343_1965"
                     /note="Signal peptide predicted for BF2049 by SignalP 2.0
                     HMM (Signal peptide probability 0.968) with cleavage site
                     probability 0.652 between residues 22 and 23"
     misc_feature    complement(2505..2537)
                     /locus_tag="BF9343_1965"
                     /note="PS00013 Prokaryotic membrane lipoprotein lipid
                     attachment site."
     gene            complement(2561..3727)
                     /locus_tag="BF9343_1966"
     CDS             complement(2561..3727)
                     /locus_tag="BF9343_1966"
                     /old_locus_tag="BF2050"
                     /note="Similar to Bacteroides thetaiotaomicron
                     acyltransferase BT1546 SWALL:AAO76653 (EMBL:AE016932) (335
                     aa) fasta scores: E(): 9.9e-09, 25.59% id in 336 aa, and to
                     Pseudomonas aeruginosa domain of unknown function Duf33
                     SWALL:Q8KIR7 (EMBL:AF498413) (629 aa) fasta scores: E():
                     1.2e-08, 26.19% id in 313 aa, and to Pseudomonas aeruginosa
                     domain of unknown function Duf33 SWALL:Q8KI23
                     (EMBL:AF498410) (629 aa) fasta scores: E(): 2.1e-08, 26.19%
                     id in 313 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transmembrane acyltransferase"
                     /protein_id="CAH07747.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1966"
                     /db_xref="EnsemblGenomes-Tr:CAH07747"
                     /db_xref="GOA:Q5LDR0"
                     /db_xref="InterPro:IPR002656"
                     /db_xref="UniProtKB/TrEMBL:Q5LDR0"
                     /translation="MKEIFEKLHRPINPAGYITEIDGFRFFAIFTVCLLHLNNFFGRSI
                     GYDYYQGVKDINSWSWFINRCGLGVELFFAISGFVIALPFFRHYLEGSKKPSLKQYFYR
                     RLTRLEVPFILSCVIIYLAYAITSQTNILADINHLFASITYTHTLIYGVWPPFNPVIWS
                     LEVEIQFYVLAPWLILFLFSGSGKYSWYIRITILFCFSLILKGFFYNELKQLHLHLSIL
                     NHLHYFLVGFTIAYLFLRQKELLYRKYFIWDIVGSICFYLLFYFIWNPNQLYFCIALFL
                     LFLSVFKGYFINWICRRKVLYIIGGMCYTIYLLHYPLFHFTGKLTKKILPFDFYYSNYL
                     IQATLLLPIVFTICCCYYILIEKPCMNKNWPQTLNAKIKRITYFKIKS"
     misc_feature    complement(2624..3682)
                     /locus_tag="BF9343_1966"
                     /note="Pfam match to entry PF01757 Acyl_transf_3,
                     Acyltransferase family, score 44.1, E-value 2.6e-10"
     misc_feature    complement(order(2645..2713,2771..2830,2843..2911,
                     2924..2992,3011..3079,3092..3160,3179..3247,3329..3397,
                     3458..3526,3593..3661))
                     /locus_tag="BF9343_1966"
                     /note="10 probable transmembrane helices predicted for
                     BF2050 by TMHMM2.0 at aa 23-45, 68-90, 111-133, 161-183,
                     190-212, 217-239, 246-268, 273-295, 300-319 and 339-361"
     gene            complement(3724..4575)
                     /locus_tag="BF9343_1967"
     CDS             complement(3724..4575)
                     /locus_tag="BF9343_1967"
                     /old_locus_tag="BF2051"
                     /note="Similar to Bacteroides thetaiotaomicron
                     glycosyltransferase involved in cell wall biogenesis BT1181
                     SWALL:AAO76288 (EMBL:AE016930) (283 aa) fasta scores: E():
                     3.6e-57, 51.41% id in 282 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="CAH07748.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1967"
                     /db_xref="EnsemblGenomes-Tr:CAH07748"
                     /db_xref="GOA:Q5LDQ9"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ9"
                     /translation="MKWYTKYLQVYEKPFSEAPQEIVEEVRNKLKLLQSDNPLVTVSLI
                     GYNEEKHLLASLWSLSEMKCKYPIEIIGTDNDSKDQTATIYEATGIPYVIEHQHSCGHA
                     RLCGLKRAKGKYHINIDSDTMYPPHYVETMVDALERPGIVAVSSLWSYIPDKEHSWVGL
                     KIYEFIRDIHLWIQSIKRPELSVRGLVFAYRTDYARQVGIRTDIRRGEDGSLALRLKEY
                     GNIYFIRKRKARAITGYGTISADGSLFNSFKVRAIYALKNIGRVFTRKKEYKDEDDNLI
                     KS"
     misc_feature    complement(3970..4455)
                     /locus_tag="BF9343_1967"
                     /note="Pfam match to entry PF00535 Glycos_transf_2,
                     Glycosyl transferase, score 56.0, E-value 6.9e-14"
     gene            complement(4588..5739)
                     /locus_tag="BF9343_1968"
     CDS             complement(4588..5739)
                     /locus_tag="BF9343_1968"
                     /old_locus_tag="BF2052"
                     /note="Similar to Yersinia enterocolitica
                     lipopolysaccharide biosynthesis WbcM protein SWALL:Q56916
                     (EMBL:Z47767) (358 aa) fasta scores: E(): 4.7e-17, 28.53%
                     id in 382 aa, and to Bacteroides thetaiotaomicron putative
                     glycosyltransferase BT1180 SWALL:AAO76287 (EMBL:AE016930)
                     (384 aa) fasta scores: E(): 4.3e-72, 49.6% id in 383 aa,
                     and to Bacteroides thetaiotaomicron glycosyltransferase
                     BT0053 SWALL:AAO75160 (EMBL:AE016926) (374 aa) fasta
                     scores: E(): 4.6e-50, 38.99% id in 377 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="CAH07749.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1968"
                     /db_xref="EnsemblGenomes-Tr:CAH07749"
                     /db_xref="GOA:Q5LDQ8"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ8"
                     /translation="MKNNIKIAYCIPSLYYPSGMERVLTLKANYFTEFFGYEIHIILTD
                     GENRPPYYELHPNITIHQLGINYDHLYGTPIYRRILEYFKKQRVFRRRLNECLNEIKPN
                     ITISLLRRDINFINKMTDGSIKLGEIHFNKSNYREFNPPGLPNFIKKLIKRFWMKQLIC
                     KLRSLERFIVLSHEDATEWTELNNVTVIHNPLSFIPESHSDCSRKQVIAVGRYMPQKGF
                     DRLISAWKIVSQKHPDWILRIYGDGMREKLQKQIDTLDIGKTCILEHSVRNIIEKYCES
                     SIFVLSSHFEGFGMVITEAMVCGVPPIAFACPCGPRDIIENNIDGILVRNGDIIGLADK
                     ICFLMENESVRKEMGIRARHNVERFKIENIALQWKNLFESVTN"
     misc_feature    complement(4657..5163)
                     /locus_tag="BF9343_1968"
                     /note="Pfam match to entry PF00534 Glycos_transf_1,
                     Glycosyl transferases group 1, score 151.0, E-value
                     1.8e-42"
     misc_feature    complement(4804..4836)
                     /locus_tag="BF9343_1968"
                     /note="PS00013 Prokaryotic membrane lipoprotein lipid
                     attachment site."
     gene            complement(5736..6770)
                     /locus_tag="BF9343_1969"
     CDS             complement(5736..6770)
                     /locus_tag="BF9343_1969"
                     /old_locus_tag="BF2053"
                     /note="C-terminus has limited similarity to C-terminus of
                     Bacteroides thetaiotaomicron putative glycosyltransferase
                     BT2938 SWALL:AAO78044 (EMBL:AE016938) (408 aa) fasta
                     scores: E(): 0.0028, 32.6% id in 92 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="CAH07750.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1969"
                     /db_xref="EnsemblGenomes-Tr:CAH07750"
                     /db_xref="GOA:Q5LDQ7"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ7"
                     /translation="MKRVLFVNNYDMIKSRESYNNGNSASHHQFGTNELLETGEYAIDY
                     MLITPKNHKNKIFKLVSLLPLWFKLYWKARNYDIVYGGADFTVDFLGILKQIKLFRPKL
                     IAIFYHPPFTIRLKLEQYDHLIFISQFAHQAMCKELPQKANMLEFMQWGPDHKFYERLA
                     PILNFQRQYENIIFISNGKTRRDHETLVSAAEKTASHTIIVSDNQSIPTNYKEGKYTQI
                     HIQEKPDDTRMVPLLNSCSVLVIPTPPYEHPLAAIGMTSFVDALALGMPIIAADNTAYR
                     DIIIENNMGKIYKAGDVDDLANAMNYFKKSPERIVECGKNAWLFGKKNDINHFGQKLRK
                     LFES"
     misc_feature    complement(5796..6299)
                     /locus_tag="BF9343_1969"
                     /note="Pfam match to entry PF00534 Glycos_transf_1,
                     Glycosyl transferases group 1, score 25.2, E-value 7.8e-07"
     gene            complement(6791..7909)
                     /locus_tag="BF9343_1970"
     CDS             complement(6791..7909)
                     /locus_tag="BF9343_1970"
                     /old_locus_tag="BF2054"
                     /note="Similar to Bacteroides thetaiotaomicron conserved
                     hypothetical protein BT1178 SWALL:AAO76285 (EMBL:AE016930)
                     (376 aa) fasta scores: E(): 2.9e-74, 52.09% id in 382 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="conserved hypothetical protein"
                     /protein_id="CAH07751.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1970"
                     /db_xref="EnsemblGenomes-Tr:CAH07751"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ6"
                     /translation="MKALFLVFHGFDEANGISKKIQHQIKALKECGLDVCTCYYEQTSD
                     GHWKWIADNNILVDFGHSTWGKIRKRICFKPIRDFIISEGISFIYIRSFHNANPFTIQL
                     VSQLKKHDVKVVMEIPTYPYDQEYITFKMKLDLLVDKCFRHHLAKLLDGIITFSDAKTI
                     FGGPTIRISNGIDFDSILPKKQINDTTYELHLIGVAEIHYWHGYDRIIKGLAEYYLTKP
                     QYKVYFHIVGELSGERERQEILPIIKQNALEPYVILYGNMHGNDLDQLFSKADMGIGSL
                     GRHRSGITHIKTLKNREYAARGIPFVYSEIDADFDHCNYVLKVPANETPINIHELISFY
                     HSQSWNINVIRHSVENLSWKMQMQKVINETYK"
     gene            complement(7906..9360)
                     /locus_tag="BF9343_1971"
     CDS             complement(7906..9360)
                     /locus_tag="BF9343_1971"
                     /old_locus_tag="BF2055"
                     /note="Similar to Shigella boydii O-antigen flippase Wzx
                     SWALL:Q93CS0 (EMBL:AF402314) (470 aa) fasta scores: E():
                     6.6e-35, 33.33% id in 462 aa, and to Bacteroides
                     thetaiotaomicron putative polysaccharide biosynthesis
                     protein BT1177 SWALL:AAO76284 (EMBL:AE016930) (491 aa)
                     fasta scores: E(): 6.4e-143, 75.88% id in 481 aa, and to
                     Streptococcus pneumoniae putative Cps repeating unit
                     transporter Wzx SWALL:Q8KWP3 (EMBL:AF402095) (474 aa) fasta
                     scores: E(): 3.6e-58, 33.76% id in 459 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|B0RRI5|2.A.66.2.23"
                     /protein_id="CAH07752.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1971"
                     /db_xref="EnsemblGenomes-Tr:CAH07752"
                     /db_xref="GOA:Q5LDQ5"
                     /db_xref="InterPro:IPR002797"
                     /db_xref="InterPro:IPR029303"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ5"
                     /translation="MTSLKHQLFSGVFYTALAKYSGILVSLVVAGVLARLISPDDFGVM
                     AVATVIIAFFNLFTDVGLSPAIIQHKSLTKENLSGLFSFTVWTGIGLAFLFTVASWPIA
                     AYYNREILRILCQLLAINLFFASATIVPNALFYRNKEFKFIALRSFVIQIATGIAAVIA
                     ALCGAGLYALIIGPILSGILIFVVSIHHYPQRLKFTLGLDVLRQIFSYSAYQFLFNIIN
                     YFSRNLDKLLIGKYMGMSPLGYYEKSYRLMMLPLQNITQVITPVMHPIFSDYQNDLERL
                     ASGYERIVRFLAFIGLPLSVLLYFTASEVTLIIFGDQWIPSIPIFQILTLSVGIQIILS
                     SSGSIFQAAGDTKSLFVCGLFSSALNVTGILLGIFWIGTLEAVASCITLTFTVNFIQCY
                     WIMYQVTLHRSLRNFVAQLISPLVISILLTAVLYPLSLLTEGGNIFLTLIVKSIIFFII
                     FGCYIQLTGEYDIIGKTKSIINKNKE"
     misc_feature    complement(order(7966..8034,8062..8121,8158..8217,
                     8230..8298,8323..8391,8419..8487,8794..8862,8872..8931,
                     8950..9018,9061..9129,9163..9231,9259..9327))
                     /locus_tag="BF9343_1971"
                     /note="12 probable transmembrane helices predicted for
                     BF2055 by TMHMM2.0 at aa 12-34, 44-66, 78-100, 115-137,
                     144-163, 167-189, 292-314, 324-346, 355-377, 382-401,
                     414-433 and 443-465"
     misc_feature    complement(8536..9339)
                     /locus_tag="BF9343_1971"
                     /note="Pfam match to entry PF01943 Polysacc_synt,
                     Polysaccharide biosynthesis protein, score 126.0, E-value
                     5.8e-35"
     gene            complement(9442..10395)
                     /locus_tag="BF9343_1972"
     CDS             complement(9442..10395)
                     /locus_tag="BF9343_1972"
                     /old_locus_tag="BF2056"
                     /note="Similar to Streptococcus agalactiae putative
                     capsular polysaccharide protein CpsI SWALL:Q9RPC3
                     (EMBL:AF163833) (322 aa) fasta scores: E(): 3.7e-19, 29.11%
                     id in 316 aa, and to Bacteroides thetaiotaomicron putative
                     glycosyltransferase BT1176 SWALL:AAO76283 (EMBL:AE016930)
                     (286 aa) fasta scores: E(): 2.3e-52, 51.23% id in 283 aa,
                     and to Clostridium perfringens capsular polysaccharide
                     biosynthesis protein CPE0501 SWALL:Q8XN37 (EMBL:AP003187)
                     (326 aa) fasta scores: E(): 1.5e-32, 40.96% id in 249 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P75905|4.D.1.1.3"
                     /protein_id="CAH07753.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1972"
                     /db_xref="EnsemblGenomes-Tr:CAH07753"
                     /db_xref="GOA:Q5LDQ4"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ4"
                     /translation="MEQCNERPKVSVIIPVYNTCNYVQEALESICQQTLEELEIIVIDD
                     GSTDCSRAIVEEIAIKDTRIQVYKQSNQGLSITRNQGLKFSTGEYIYFMDSDDFLEPDA
                     LELCYRKCTKEALDFVFFDAETFTIEPNIPESSFDYQRTLDLPEKICSGIDMLKTQLKT
                     YKFKSSVCLNLFHREFICEERLSFYPGILHEDQLFMIQAYLHASKTGFIRRSFFHRRIR
                     TNSIMTKKFTWKNMEGYLTVTNELLRELPIYPKTYHHTIFFFLRQMLDAAVWQAHTLSL
                     PQRIKLFSLCLKYYRPYVTMRTLMVLLLKTSIHPRQ"
     misc_feature    complement(9898..10365)
                     /locus_tag="BF9343_1972"
                     /note="Pfam match to entry PF00535 Glycos_transf_2,
                     Glycosyl transferase, score 132.5, E-value 6.7e-37"
     gene            10476..10649
                     /locus_tag="BF9343_1973"
                     /pseudo=""
     CDS             10476..10649
                     /locus_tag="BF9343_1973"
                     /old_locus_tag="BF2057"
                     /note="Pseudogene. Similar to Bacteroides thetaiotaomicron
                     N-acetylmuramoyl-L-alanine amidase BT1168 SWALL:Q8A8J9
                     (EMBL:AE016930) (147 aa) fasta scores: E(): 9.6e-09, 62.5%
                     id in 48 aa. Note This CDS contains a stop codon at residue
                     50"
                     /pseudo=""
                     /protein_id="Q8A8J9"
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmuramoyl-L-alanine amidase
                     (pseudogene)"
     gene            complement(10785..12005)
                     /locus_tag="BF9343_1974"
     CDS             complement(10785..12005)
                     /locus_tag="BF9343_1974"
                     /old_locus_tag="BF2058"
                     /note="Similar to Salmonella enterica O-antigen WbbF
                     protein SWALL:Q52257 (EMBL:L39794) (459 aa) fasta scores:
                     E(): 2e-10, 23.36% id in 351 aa, and to Bacteroides
                     thetaiotaomicron conserved hypothetical protein BT1167
                     SWALL:AAO76274 (EMBL:AE016930) (395 aa) fasta scores: E():
                     2.2e-66, 60.96% id in 415 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glyco_tranf_2_3"
                     /protein_id="CAH07755.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1974"
                     /db_xref="EnsemblGenomes-Tr:CAH07755"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ3"
                     /translation="MILILTLIDWILFVPLALCVAYLLVYAIASKFYRAPHYPEADKLH
                     RIAVFFPAYREDKVIIDSVRSFLEQDYPKEMYDVYVISDHMQDSTNKALSRLPIRLLVA
                     TYEDSSKAKALLLGISTIEEDGKAKGLGNRQLAFMYDIAVVMDADNVTVPGFLSEVNRA
                     YSAGVQAMQAHRTGKNLNTDIALLDGVSEEINNGFFRSGHNALGLSAGLAGSGMAFDYF
                     WYYDAVQSLETAGEDKELELTLLECRMHTVYLENLPVYDEKTQKKENIKNQRRRWMAAQ
                     FGILCEGLSFIKSVKQMEGWWRWWPSFDLVDKIIQWMLPPRLVQLVAVFGFTLLTTLVH
                     RPVASKWWILSAAQVAAMFIPVPARLLNGRLLKALMQVPSLALGTIASLFHLKGANKKF
                     IHTEHGE"
     misc_feature    complement(11340..11864)
                     /locus_tag="BF9343_1974"
                     /note="Pfam match to entry PF00535 Glycos_transf_2,
                     Glycosyl transferase, score 8.8, E-value 0.00084"
     misc_feature    complement(11919..11987)
                     /locus_tag="BF9343_1974"
                     /note="1 probable transmembrane helix predicted for BF2058
                     by TMHMM2.0 at aa 7-29"
     gene            complement(12219..13109)
                     /locus_tag="BF9343_1975"
     CDS             complement(12219..13109)
                     /locus_tag="BF9343_1975"
                     /old_locus_tag="BF2059"
                     /note="Similar to Sphingomonas sp. S88 glycosyl transferase
                     SpsQ SWALL:P74817 (EMBL:U51197) (315 aa) fasta scores: E():
                     4.3e-13, 24.75% id in 307 aa, and to Bacteroides
                     thetaiotaomicron putative glycosyltransferase BT1166
                     SWALL:AAO76273 (EMBL:AE016930) (299 aa) fasta scores: E():
                     7.5e-81, 69.01% id in 284 aa, and to Clostridium
                     acetobutylicum predicted glycosyltransferase CAC3069
                     SWALL:Q97EN9 (EMBL:AE007804) (299 aa) fasta scores: E():
                     3.1e-29, 34.23% id in 260 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="CAH07756.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1975"
                     /db_xref="EnsemblGenomes-Tr:CAH07756"
                     /db_xref="GOA:Q5LDQ2"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ2"
                     /translation="MKQTPPLISFITICYNGLDDTCMLIESLRDTISSVSYEIIVVDNA
                     SRQDEASLIQERYPFVRTLRSEKNLGFSGGNNLGIQIAQGKYLFLINNDTYLTEDGLPA
                     LIERLESDPRIGAVSPKIRFAFPPQSIQFAGYTKLSRYTMRNKALGMGCPDDGTFDTPR
                     PSAYLHGAALMLKREVIWKAGLMPEIYFLYYEELDWCTSMTRAGYQLWYEPRCTIFHKE
                     SQSTGRQSPLRTFYMMRNRMLYAWRNLPGMERCLAIAYQMLIVAPKDSLCFVLKGEPKL
                     AKAVWHGVKGFWKLC"
     misc_feature    complement(12558..13085)
                     /locus_tag="BF9343_1975"
                     /note="Pfam match to entry PF00535 Glycos_transf_2,
                     Glycosyl transferase, score 90.1, E-value 3.9e-24"
     gene            complement(13152..14486)
                     /locus_tag="BF9343_1976"
     CDS             complement(13152..14486)
                     /locus_tag="BF9343_1976"
                     /old_locus_tag="BF2060"
                     /note="Similar to Salmonella muenchen O-antigen polymerase
                     Rfc SWALL:RFC_SALMU (SWALL:Q00474) (399 aa) fasta scores:
                     E(): 3.5e-05, 23.96% id in 338 aa, and to Bacteroides
                     thetaiotaomicron hypothetical protein BT1165 SWALL:AAO76272
                     (EMBL:AE016930) (455 aa) fasta scores: E(): 1.1e-116, 62.2%
                     id in 426 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transmembrane surface-related protein"
                     /protein_id="CAH07757.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1976"
                     /db_xref="EnsemblGenomes-Tr:CAH07757"
                     /db_xref="GOA:Q5LDQ1"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ1"
                     /translation="MNNEEEIYNRDPHQSSRSFYVLFALQFLPILASIFIEVKLGIATL
                     AIIVPMTFITLIACSNRQTDWSHSRNIILLLFFIWGIYCLLEISNPNNVQEAWNISIGH
                     YLFIPIICAVLVPISIRDRKGIESLLFIWGIFILIVTIKGYWQKNHGFTDKELYFLYTL
                     GGARTHIIWSGIRYFSCFSDAANYGVHSAMAASAFGIAMFYVQKRWMKVFFAFIMICGI
                     YGIGISGTRAAVAVPIGAILMYVLISKNTKSIITSSVILIGVFSFFYFTNIGNGNQYIR
                     KMRSAFRPTEDASYQVRVENRKKMKVLMDERPMGYGLGLSKGGRFNPKEQMPYPPDSWL
                     VSVWVESGIIGLTLYLLIHGALFAWCSWILMFKIMNQRLRGLLAAWLCTNAGFFIAAYA
                     NDVMIYPNPFIIYTGFALCITATNIDPVMQKEDDEMNREKKEIDY"
     misc_feature    complement(order(13215..13283,13293..13352,13371..13439,
                     13668..13727,13746..13844,13872..13931,13950..14018,
                     14061..14114,14133..14192,14220..14273,14310..14369,
                     14379..14432))
                     /locus_tag="BF9343_1976"
                     /note="12 probable transmembrane helices predicted for
                     BF2060 by TMHMM2.0 at aa 19-36, 40-59, 72-89, 99-118,
                     125-142, 157-179, 186-205, 215-247, 254-273, 350-372,
                     379-398 and 402-424"
     misc_feature    complement(14310..14342)
                     /locus_tag="BF9343_1976"
                     /note="PS00013 Prokaryotic membrane lipoprotein lipid
                     attachment site."
     gene            complement(14479..16695)
                     /locus_tag="BF9343_1977"
     CDS             complement(14479..16695)
                     /locus_tag="BF9343_1977"
                     /old_locus_tag="BF2061"
                     /note="Similar to Bacteroides thetaiotaomicron conserved
                     hypothetical protein BT1164 SWALL:AAO76271 (EMBL:AE016930)
                     (742 aa) fasta scores: E(): 3.9e-156, 58.35% id in 742 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transmembrane protein"
                     /protein_id="CAH07758.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1977"
                     /db_xref="EnsemblGenomes-Tr:CAH07758"
                     /db_xref="GOA:Q5LDQ0"
                     /db_xref="UniProtKB/TrEMBL:Q5LDQ0"
                     /translation="MDYILYLFRSLYRVKWWILLGTLIISAIAYYLTGNMKGGYNVEAT
                     LYTGVVSGYSIEENTKVDWALAQNSMDNLINIIQAESTLKRVSLRLFSRVLVKGDPNKD
                     NEGITSACYNFTYNHMKNSKDGKTLISLIDRSSEDKTMENFQKYERPDMNNYIYGLFYF
                     QHIYYSYKALKNIKVDRKGSSDLLRVSYQSGDPGIAYNTVEILMKEFVNEYQALRYGGT
                     DKVIEYFRSELKRIGKELTNYEDDLTQYNVTNRIINYYDETKEIAAINKEFELREQDVL
                     FNYNSSKAMLNELEKQMDSNIKRVIHNVQLVDKINQASDLTGKITQMETISTSSEDSGR
                     QLLEYKNKLLQTRRDLSEISNQYVAGQYTKEGLNKGTIVEQWLDQLLLFEKAKAELKIV
                     QKSRNELNAKYLHFAPVGTTIKRKERIINFTEQNYLTNLKSYNDALLRKKSLEMTAAIL
                     KVLNPPAYPINSESTNRRKIVMMIGVGSFIGLIAFFLLIEFIDRTLRDSVRTRKQTGSP
                     VLGAYPAPMKFSPISKQCEDIATRYMSSSILRFFTQKKEGMPYILNMLSTEDGSGKTYL
                     ANRLKAYWEGIGLHVRMLSDGTDFDSNSSKYALANTITDLYIPQEEDILIIEQPSLNRA
                     NIPVSILQDAQLNLVIASADHGWKDIDKMLLQKLKAQLGKAPYLYLNWAPKYEVETYTG
                     MLPPYTFLHKQLYRLSQLALTESFIRWKKNSRKNYQDDDNDDDE"
     misc_feature    complement(order(15196..15264,16594..16662))
                     /locus_tag="BF9343_1977"
                     /note="2 probable transmembrane helices predicted for
                     BF2061 by TMHMM2.0 at aa 12-34 and 478-500"
     gene            complement(16706..17455)
                     /locus_tag="BF9343_1978"
     CDS             complement(16706..17455)
                     /locus_tag="BF9343_1978"
                     /old_locus_tag="BF2062"
                     /note="Similar to Bacteroides thetaiotaomicron conserved
                     hypothetical protein BT1163 SWALL:AAO76270 (EMBL:AE016930)
                     (249 aa) fasta scores: E(): 1.3e-58, 66.53% id in 248 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative outer membrane protein"
                     /protein_id="CAH07759.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1978"
                     /db_xref="EnsemblGenomes-Tr:CAH07759"
                     /db_xref="GOA:Q5LDP9"
                     /db_xref="InterPro:IPR003423"
                     /db_xref="UniProtKB/TrEMBL:Q5LDP9"
                     /translation="MKRVLFILCSIPLLLFSPVSFANAQETSDFSKLNPEDYTNISLPP
                     LDLLFENAKGGPIYELASVKEQIERKLLAKERRSVLSFFNIRGSYQWGKFGNDYTFTDV
                     STPILYNYSTSKQQTYTVGAAVNIPLDELFDLAPRVRRQKLNVKTAALEREAKFEDMKK
                     EIIELYATATSQLSVLTLRAEALELATVQYSIAEKDFANGTIESSTLSVEKGRQSTALE
                     AFEKSRFELTKSLMILEVITRTPILRK"
     misc_feature    complement(16841..17119)
                     /locus_tag="BF9343_1978"
                     /note="Pfam match to entry PF02321 OEP, Outer membrane
                     efflux protein, score 19.5, E-value 1.2e-05"
     misc_feature    complement(17384..17455)
                     /locus_tag="BF9343_1978"
                     /note="Signal peptide predicted for BF2062 by SignalP 2.0
                     HMM (Signal peptide probability 1.000) with cleavage site
                     probability 0.545 between residues 24 and 25"
ORIGIN
        1 acgaaggtag ttaacacctt tcattacgtt aatgttcata aacaggaaat aatacgggat
       61 gaagagcagc ttgtttttca cttgccgggt agaaaggtag tagccccatc ctcccatgat
      121 gtagaatagg agctggagtg ccaggatggt gccgtaacag acaggggagc cccccgccag
      181 tagaatggcg atgttgagtg gaagcagcgc gaacagcagc accggtgcca gagaccacct
      241 cagcactcta tgcgatacgt attgaaacgt cagcactccg taacggaacg gattgagcag
      301 ttctctcagc cgccagatag attgcagtcc accggctgcg atacgcactt tccgcttctg
      361 ctcctcgcgt atgtcggccg atccgctttc gatggcatac gcctgcgtgc aatatgctat
      421 cttgtatccc tgcatggcga tgcgcaacga caggatgaag tcgtccagca gcgtgtccgg
      481 ttccatcact gtgaagagct cccggcgtac ggcaaatagt tcgcctgcgg ctcccacggc
      541 ggagtagagg cgtgcatcca gtgctttcag tgtcgattca tatttccagt atatgccttc
      601 tcctccggcc gccgcaccgt ctttttcttg cacggctatc cttttctcac cggcaacgca
      661 tcccaccttc gggtcttcaa aagcccggac tatttcgcgt attgcttcgc ggttgagcat
      721 cgtattggca tcggtaaaca ctacgagcgg cgtgtccacc agcatcatgc cccgtgtcat
      781 ggcggccgtt tttccttgcc gcaggggttg gaagtggacg gtggcctgcg gccagtcttc
      841 cagtcgctgg ttggtagagt cgttgcttcc atcggtggtc cacacgatgt gcagcttgtc
      901 cgccgggtag tccagcccga gacagttgcg catcttctcg tccaccacct cttcttcgtt
      961 aaaggctgtg atgaagagtg tcacgtccgg cagtcttttg tccgggggga gctgtcgccg
     1021 gacaggtttc acaaagagtt ccttcagttt gaccagcatg taaagcagta ttccgtatcc
     1081 caggtgggta tagaatacga ggaagaggga gagccagaaa aggatttgca gtgttgtcat
     1141 catagtatct cttattttat tcggttcaat aattcggctt tcttacttga ttctttaagc
     1201 atgtttacaa agtagagtgc gtctgctttg ttcgattggt ttgttttgtc tttttcatat
     1261 tgctctacaa agtgcgtagc gtgttcaacc aggaaccgtc ggcagtcaat gttcagcatg
     1321 actccgtcct gataatttcc gtaagacagt ccggtgacag cactgttact ttctatcgac
     1381 ttgatgtctt ctttcttcag tcctttcagg ttgatggtga ctgaagggta ataaaaatat
     1441 tcctggctcg gcaagttgat gatcaatttc aatgtcgatc cgtctgcgct cttttcaatc
     1501 tttccatgca tgcgataata gttgtattca taatattctt cttgactagg catccatata
     1561 caatcttcac catctttgcc ataagtgtcg ttcagccaaa gaagaaagtc agtccagaag
     1621 gcatctgtag aatgtacacc gatgttaata gcgcaccgat ccacagtggg tatagatcgt
     1681 tcttgctcta tcacctgttt aaagtcatca ggactatcat gaaagactct atttaatgta
     1741 tgattttcta gattagtaag attatgaaat ggatatacct tttcaccacg ttctgctgtg
     1801 atagttttta tatcatctaa gttaattgca gcagtagtat aatcaatgtt tccatcaggt
     1861 tctgaaagcg ttttacattt cctgcccgat aatctttttt gtatgctatc ttgcccaata
     1921 agataatgtt tttgtatgct atctatgtta tgtatttcct tagtatttac gttatgaaac
     1981 gctatactat tgccataatt aactatatca attacatctt cccataaaat gccatttctt
     2041 ccataaaaac gataattgtc tttgctatat cccaaattta ttattgattc ttcggccatg
     2101 tagttatcgt ctgcagccaa tgtagctgta aatgaaaatc taacttcttt gcctgttcca
     2161 tcagtacatc ctaaggtttt tcctaaatag taaatatctg gtggaagatc atcttgcttt
     2221 aattgttcat aatgatagta atattgtccc gaaagaggct ttccattgat acttgcccat
     2281 gttcgtggat agatactagg cttacaatcg tcttgggtaa gcataattag caaatgtttg
     2341 ttatatttta gaggtgcaaa ataaacatct aacaattcta aattgatagt tgaattggtt
     2401 ttgattgtaa tctcggctgt cacattttta ttttcttgat caaaagggta taaatatgcc
     2461 ggttctctta aacttcctcc tttatacaag tcaacttctt tgatacatga agctgctaat
     2521 agtatcaaaa ggaatattgc tatggtttta atgtatattt tcatgacttt attttaaaat
     2581 aggtaatacg tttaattttt gcattgagtg tttgtggcca atttttgttc atacaaggct
     2641 tctctattaa tatatagtaa caacaacata ttgtaaatac aattggaagt aataatgtag
     2701 cttgtattaa atagttcgaa tagtaaaagt caaatgggag tattttttta gttagtttgc
     2761 ctgtaaaatg aaataatgga taatgtagta aataaattgt gtagcacatt cctcctatta
     2821 tgtataatac ttttcttcta catatccaat ttataaaata tcctttgaag acacttagaa
     2881 ataacaaaaa caatgctata caaaagtata gttgattagg attccatata aaatagaaga
     2941 gtaaataaaa gcagatggat ccaacaatgt cccatatgaa gtattttcta tataacagtt
     3001 ctttctgtcg taagaataaa tatgcaatgg taaaccctac aagaaaataa tgaagatggt
     3061 ttaatataga taggtgtaaa tgtaattgtt tcaattcatt ataaaagaaa ccttttaata
     3121 ttaatgaaaa acaaaataat atagtgatgc gaatatacca tgaatatttt cctgaaccgg
     3181 aaaaaagaaa taaaattaac catggagcca atacgtaaaa ctgaatttcc acttcaagac
     3241 tccatatgac tggattaaaa ggaggccata caccataaat tagagtgtgc gtataggtta
     3301 ttgatgcgaa taaatggttt atatccgcaa gtatatttgt ttgagaagtt atggcatatg
     3361 ccaaatatat gatgacacat gataaaataa aaggtacttc taagcgagtt aaccttctat
     3421 agaaatattg ttttaaagaa ggttttttac tgccttctaa ataatgacgg aaaaaaggta
     3481 atgctatgac aaatccacta attgcaaaaa aaagctcaac acctaaacca catcgattaa
     3541 tgaaccaact ccatgaattg atatctttga ctccttggta ataatcatat ccgatacttc
     3601 tgccaaaaaa attattgaga tgcaacaggc agactgtaaa gatagcaaag aaacggaatc
     3661 catctatttc agtgatatac ccagcggggt taattggacg atgtaatttc tcgaatattt
     3721 ctttcatgat tttattaaat tatcatcttc atccttatat tctttctttc gcgtaaatac
     3781 tctacctata tttttgaggg cgtaaatagc ccgtacctta aaactgttga ataacgagcc
     3841 gtcagcgctg atggtaccat atcccgtgat tgcacgtgcc tttcttttgc gtataaaata
     3901 tatatttccg tattctttga gtcgtaaagc taaagagcca tcctcccccc gtctaatatc
     3961 tgtgcggata cccacttgac gagcgtaatc tgtacggtat gcaaatacca gtcctcgtac
     4021 acttaattca ggacgtttga tcgattgtat ccataaatgg atgtctctaa tgaattcata
     4081 tatttttaat ccgacccacg aatgttcttt atcgggaata tagctccata atgaacttac
     4141 tgctacaatt ccaggacgtt ctaatgcatc aaccattgtt tcaacataat gtgggggata
     4201 catagtgtcg gagtcaatat taatgtgata ttttcctttg gcacgcttta atccgcagag
     4261 gcgagcatgt ccacaactat gttgatgctc tattacatat ggaatgccgg ttgcttcata
     4321 gattgttgct gtttggtctt tggaatcgtt atctgtacca ataatttcta ttggatattt
     4381 acatttcatt tcactcagag accataaact cgctagtaga tgtttttctt cattatatcc
     4441 tataagagag actgttacaa gcggattgtc agattgtagt aatttcagtt tatttcgaac
     4501 ttcttcaacg atctcttgtg gagcttcaga aaaaggcttc tcgtatactt gtaaatattt
     4561 tgtgtaccat ttcataatat ttttctttta attagtaact gattcaaata agtttttcca
     4621 ttgaagtgct atgttttcta ttttgaagcg ttctacgttg tggcgtgcac gaattcccat
     4681 ttctttacgt acactctcat tctccataag gaaacagatt ttatctgcta atcctattat
     4741 atctccattt cttactaata taccatcaat attattttca atgatatcac gtggtccaca
     4801 tggacaagca aatgctatag gaggtactcc acataccatt gcttctgtga tgaccattcc
     4861 aaatccttcg aaatgtgaag atagaacaaa tatagaactt tcacaatatt tctctatgat
     4921 attccgtaca ctgtgttcta atatacaggt tttgccaata tctaacgtat caatttgttt
     4981 ttggagtttt tcgcgcatac catcaccata tatgcgcaat atccagtccg gatgtttttg
     5041 ggatacaatt ttccatgcag atattagacg atcaaatcct ttttgtggca tatatcttcc
     5101 aactgcaatc acttgttttc tgctgcagtc agaatgtgac tctggaataa atgaaagagg
     5161 gttgtgaata acggttacat tatttaattc tgtccactct gttgcatctt catggctaag
     5221 aacaataaac cgctctaatg aacgtaactt acatataagt tgtttcatcc aaaaacgttt
     5281 gattaatttt tttataaagt tgggaagtcc aggaggatta aattcgcgat agtttgattt
     5341 attaaaatgt atctccccta attttatgct tccgtctgtc atcttattga taaaattaat
     5401 atctcgtctc aataatgata ttgtaatgtt gggttttatt tcatttagac attcgttcaa
     5461 gcgtcttcga aagactctct gttttttaaa gtattctaag atgcgcctat agataggtgt
     5521 tccatataga tgatcgtaat tgattccaag ttgatggatt gtaatattgg gatgcaactc
     5581 atagtatgga ggtctatttt ctccatccgt taaaatgata tgtatctcat agccaaagaa
     5641 ctcagtaaag taattggcct ttaatgttaa aacccgttcc ataccacttg gatagtataa
     5701 agacggaatg caataagcta ttttgatatt atttttcatg attcaaataa cttacgtagt
     5761 ttttgtccga aatgatttat gtcattcttt ttgccaaata gccatgcatt ttttccacat
     5821 tcaacaattc tttctggtga ttttttaaaa taattcatgg catttgctaa atcatcaaca
     5881 tcccctgctt tgtagatctt tcccatgttg ttctcaatga taatatctct gtatgctgta
     5941 ttatcagctg cgataatagg catacccaag gctaatgcat cgacaaaact agtcattcct
     6001 atagctgcaa gtggatgctc atatggggga gttggaataa ctaaaacgga acagctattg
     6061 agtaagggca ccattctagt gtcatcgggt ttctcttgaa tatggatttg agtatatttg
     6121 ccttctttgt aatttgtagg aatactttgg ttgtcactaa cgataattgt atggcttgct
     6181 gtcttctcag cagcactcac taatgtctcg tggtcacgtc gggttttacc attactaatg
     6241 aatatgatgt tctcatattg tctttggaaa ttaagtatag gggctaggcg ttcataaaac
     6301 ttatgatctg gtccccattg cataaattct agcatattag ctttttgggg cagctcctta
     6361 cacatggctt gatgagcaaa ttggctgatg aaaataagat gatcatattg ctctaatttc
     6421 agccttatgg tgaatggagg gtgataaaat atggcaataa gttttggacg aaataatttt
     6481 atttgtttta atattcccaa aaagtctact gtaaaatccg ctcctccata tacaatatca
     6541 taattgcgcg ctttccaata gagtttaaac cataaaggta gaagtgacac taatttgaat
     6601 attttatttt tgtgattttt aggagtaatc agcatatagt cgatagcgta ttcaccagtt
     6661 tcaagaagct cattagttcc gaattgatga tgacttgcag agttcccatt attgtatgat
     6721 tctctggatt ttatcatgtc gtaattatta acaaataaaa cgcgtttcat gtttttatta
     6781 agtatttaat ttatttataa gtttcattga taactttttg catttgcatt ttccatgata
     6841 aattttctac ggaatgacga ataacattta tgttccatga ttggctatga taaaaagata
     6901 tcaattcatg tatattaatt ggagtttcat tggctggtac tttaagtaca taattgcagt
     6961 ggtcaaagtc tgcatcaatt tctgaataga caaacgggat acctcgggct gcatattctc
     7021 tgttttttag agtctttata tgagtaattc cacttcggtg ccgtcctaag ctacctatgc
     7081 ccatatctgc cttagaaaat agttggtcga ggtcgtttcc atgcatgttt ccatataaaa
     7141 taacataagg ctctaaggca ttctgtttga ttattggcaa tatttcttga cgttcacgtt
     7201 ctccgcttaa ttcgcctaca atgtgaaaat atactttgta ttgaggtttt gtaagataat
     7261 attctgccaa tcctttgata atacgatcat atccatgcca ataatgtatt tctgccactc
     7321 caattaaatg tagttcataa gttgtatcat ttatttgttt tttaggtagt attgaatcaa
     7381 aatcaattcc atttgagata cgaatagtag gtcctccaaa gatggttttg gcatctgaaa
     7441 atgtgatgat cccatcaagt aattttgcta aatgatggcg aaaacattta tcaaccaata
     7501 ggtcaagttt catcttgaaa gttatatatt cctgatcata agggtatgtt ggaatttcca
     7561 ttactacttt tacatcatgt ttcttaagct gactgaccaa ttgtattgta aatggattag
     7621 cattatgaaa agaacggata tatataaagc tgattccttc agaaattata aaatctctta
     7681 tgggcttaaa acatatacgt tttcttattt ttccccatgt gctatgtccg aaatcgacta
     7741 aaatgttatt gtcagctatc cacttccaat gtccatctga tgtttgttca taatagcagg
     7801 tacatacgtc taaaccgcac tcttttagtg cttttatttg gtgttggatt tttttgctga
     7861 ttccgtttgc ttcgtcgaat ccgtgaaata caagaaacag tgctttcatt ctttgttttt
     7921 gtttatgatg cttttggtct taccgatgat gtcgtattca ccggttaact gtatgtagca
     7981 cccaaatata atgaaaaata taatactctt tacaattaag gtcagaaaaa tgtttcctcc
     8041 ttctgttagt agactgagtg gatagagtac ggcagtcagc aagatgctga taacaagcgg
     8101 cgatatcagt tgagctacaa aattgcgcag gctgcgatgt agtgttactt gatacattat
     8161 ccaataacat tgtatgaaat taacagtaaa tgtaagtgtg atacatgatg ctacggcttc
     8221 aagtgtgcca atccagaaaa tacccagtaa gataccagtt acattgagcg cagatgaaaa
     8281 caaaccacac acaaataggc tttttgtatc gccagctgct tgaaaaatcg aaccagagga
     8341 agacagaata atctgaatgc ctaccgataa tgtgagtatt tgaaatatag ggattgatgg
     8401 tatccactgg tcgccaaaaa taatcaatgt tacttcgctg gctgtaaagt agagtaatac
     8461 acttaatggc agtccaatga aagccaagaa acgtacaatg cgttcgtagc ctgaagccaa
     8521 gcgttcaaga tcattttgat agtcgctgaa gatgggatgc attacgggag tgattacttg
     8581 tgtgatgttc tgtaagggta gcatcatcag gcgatacgat ttttcatagt aacctaaagg
     8641 agacatcccc atgtatttac cgattaatag tttatccagg ttacggctaa agtagttaat
     8701 aatgttaaac aggaattgat aggcagaata agagaagatt tggcgcaata catccagccc
     8761 aagtgtaaat ttcagacgtt gtggataatg atgtatggaa accacaaata ttaggatacc
     8821 cgataaaata ggacctataa ttagtgcata aagcccggct ccacataggg ctgctatgac
     8881 agctgctata ccagtggcta tctgtataac gaaacttctc aaggcaataa atttaaattc
     8941 tttattgcga taaaacaaag cattgggaac aatagtagct gaagcaaaaa ataggtttat
     9001 agctagtaat tgacatagta tgcgcaggat ttcccgatta tagtaagccg ctatgggcca
     9061 tgaggcaact gtgaaaagaa atgccagacc gatacccgtc cagactgtga aagagaagag
     9121 tcctgataaa ttctctttgg ttagactttt gtgttggata atggcaggtg atagtcctac
     9181 atcggtgaat agattgaaaa aggcaatgat tactgttgct acagccatta caccaaagtc
     9241 gtctggtgaa attaggcgtg ccaatacgcc tgctacgaca agtgatacga gtatgccact
     9301 gtatttggca agggcggtat agaatacccc cgagaatagt tggtgcttga gtgaagtcat
     9361 aaatttattt ttaaagctac tctgcttttc caatggagaa ggtgagggcg ttggatttat
     9421 aattagaaat atgttgtttc tttattgtct gggatggatg gatgttttta gtaataatac
     9481 cattagcgtt cgcatagtta catatggcct ataatatttg agacagaggc tgaatagttt
     9541 tatacgttga ggtaaagata atgtatgtgc ttgccataca gctgcatcca gcatctgtcg
     9601 taaaaaaaag aatattgtat ggtgatatgt ttttggataa attgggagtt ctcttaataa
     9661 ttcgttagtt acagtaagat atccttccat atttttccat gtgaattttt tagtcatgat
     9721 agagtttgtt ctgatacgcc tatggaagaa agaacgacga atgaatcctg ttttagaagc
     9781 atgcaaataa gcttgtatca tgaagagttg atcttcgtgt aatataccgg gataaaatga
     9841 aagtctctct tcacaaatga actctcggtg aaataagttt aggcaaaccg atgatttgaa
     9901 cttgtatgtt tttagttgtg tttttaacat gtctatgcct gaacatatct tttcaggtag
     9961 atctaaagtt ctttggtagt cgaaggagct ttcgggtatg ttcggttcaa ttgtgaacgt
    10021 ttcagcatcg aagaaaacaa aatcaagagc ttcttttgtg catttacgat aacataattc
    10081 aagtgcatca ggctctaaaa agtcatcact atccatgaaa taaatatatt ccccagtaga
    10141 aaatttcagt ccctgattac gtgttataga aagtccttga ttagattgtt tataaacttg
    10201 aatccgtgtg tccttgattg caatttcttc cacgattgct ctactacaat cggtagagcc
    10261 gtcatcaatt acgataattt ccaattcttc cagtgtctgc tgacaaatac tttccagtgc
    10321 ttcctgtacg taattacagg tgttatatac agggataata acgctaactt taggacgttc
    10381 attgcattgt tccattcttt ttgagtgtga tgatgtttca atctagcaaa gatacataaa
    10441 aagatttaag gaaaacggtt attttctcgt aaaatatgga aaagttgaga ggtaataatg
    10501 tgttgattta ctcgctgcag gtactggtgc gtatgctcct ggtagtttat cctggttacg
    10561 aaatgggtgc ccattgtgat ttgaaccccg atcagaacgg cgatggagag atagaacccg
    10621 aatagtggat caaggcgtgt ccttgtttta aggttttgct gcgggtagtg tacggttgac
    10681 ggggggtacc acagatttac actggattat acagaggatt tattttcttc gggatacccg
    10741 tgaacgagaa accgaaactc cgtgtaaatc tgtggtaacc cgtatcactc accgtgttcg
    10801 gtatgtataa atttcttatt ggcccctttg aggtggaaca ggctggctat ggtccccaat
    10861 gccagcgaag gcacctgcat tagtgctttt agtagcctac cgttgagcag gcgggctgga
    10921 acagggataa acatggcggc aacctgtgct gccgacaata tccaccattt agacgctacc
    10981 ggacggtgga ccagtgtggt aagcagagtg aatccgaaaa cggctaccag ttgcaccagg
    11041 cgcggaggga gcatccattg gatgatttta tcgaccagat cgaaagaggg ccaccaccgc
    11101 caccatcctt ccatctgctt gacgcttttt atgaaggaca gtccctcaca caagatgccg
    11161 aattgcgctg ccatccagcg ccggcgctga ttcttgatgt tctctttttt ttgcgttttt
    11221 tcgtcgtaga caggtaggtt ttccagatag acggtgtgca tcctgcactc aagtagcgta
    11281 agttccagct ctttgtcctc tcctgcggtt tccaggcttt gcacggcatc gtaataccag
    11341 aagtaatcaa aagccatgcc cgagcctgcc agtccggcag acaatccgag ggcgttgtgt
    11401 ccgctcctga aaaatccatt gttgatctcc tcgctcactc cgtcgagcaa ggcaatgtcc
    11461 gtattcaggt ttttgcctgt gcggtgggct tgcatcgcct gcactccggc ggagtaggca
    11521 cggttcacct cggaaaggaa tccgggtacg gtcacgttgt cggcatccat caccacggca
    11581 atgtcataca taaaggccaa ttggcggttg cctagtcctt tcgcctttcc gtcttcctcg
    11641 atggtgctga tgcccagtag gagggctttg gccttggaac tgtcttcgta ggtagccacc
    11701 agcaggcgga tagggagtcg gctcagggct ttgttggtgg agtcctgcat gtggtcggag
    11761 atgacgtata catcgtacat ttctttgggg tagtcctgct caaggaaaga gcgtacggag
    11821 tcaatgatga ctttgtcttc ccggtaggcc gggaagaaga cggcgatgcg gtgcaatttg
    11881 tccgcttccg gatagtgggg cgcacgatag aatttggacg caatagcgta cactagcaga
    11941 taggctacgc aaagtgccaa cggaacgaac aggatccagt cgatcaaggt tagtatgagt
    12001 atcataagtc ttaatgtagg ttatgggtcg atatatttgt gtaaatcggt tcagctgcgg
    12061 ttcaccacag attacacaga ggatcatttc ggcagagttt tacagaatcc tatagaatct
    12121 cagagagttc cacaggattt cactttctcg tttatggata tccccaaaaa aatgattcct
    12181 ctgtgtgcta atttgtgtaa tctgtggtgg actgtcactc aacacagctt ccagaaaccc
    12241 ttcactccgt gccagacggc ttttgccagc ttgggctctc ctttcaggac gaagcacagg
    12301 ctgtctttgg gagctacgat cagcatttgg taagcgatag ccaagcaacg ttccattccc
    12361 gggaggttgc gccaggcgta aagcattcgg tttcgcatca tataaaaggt gcggagaggg
    12421 ctttgccgcc cggtactttg gctctctttg tggaagatgg tgcagcgcgg ctcgtaccac
    12481 agctgatagc cggcacgcgt catgctggta caccagtcca gctcctcgta atagagaaaa
    12541 tagatctccg gcatcagtcc ggctttccag atgacctccc gtttcagcat cagcgcagct
    12601 ccgtgaaggt aggcgctggg gcgtggagtg tcgaaagtcc cgtcgtccgg acatcccatg
    12661 cccaatgctt tattgcgcat cgtataccgg ctgagcttgg tatatccggc aaattgaatg
    12721 ctttgcggcg gaaaggcaaa acggatttta ggtgatacag ccccgatgcg gggatcgctc
    12781 tccaaccgtt ctatcagggc gggcagtccg tcttcggtca ggtaggtgtc gttattgatc
    12841 aggaacaggt attttccttg tgctatctgt attccgagat tgtttcctcc cgagaatccg
    12901 aggtttttct cgctgcggag agtccgtaca aagggatacc gctcctgtat cagggaggct
    12961 tcatcctggc gggatgcgtt gtccaccaca atgatttcat aactgacgga actgatcgtg
    13021 tcgcgtagcg attctatcaa catgcaagtg tcgtccagcc cattgtaaca gatggtgata
    13081 aaagatatca aaggaggcgt ttgtttcatg aatgtttgtt tttgtgataa atcggatcca
    13141 tttttatttc gttaataatc aatctctttt ttctctctat tcatctcatc atcttctttt
    13201 tgcatgacag gatcaatatt tgtagcagtt atgcagagtg caaaacctgt ataaataata
    13261 aaaggatttg gatatatcat gacgtcattg gcgtatgcag caataaaaaa gcctgcattt
    13321 gtacaaagcc atgctgctaa taatcctctt aaacgttggt tcatgatttt aaacatcagt
    13381 atccaagagc accaggcaaa aagagctcca tgaatcagta aatataatgt caatccaata
    13441 attccacttt ctacccaaac acttactaac caagaatccg gtggataggg catttgttct
    13501 ttggggttaa atcttccacc tttggatagt cctaaaccat atcccattgg tctttcatcc
    13561 attaatacct tcattttttt tcgattctcc actcgtactt gataagaagc atcttctgta
    13621 ggacgaaaag ccgaacgcat tttacgtata tactggttgc cattaccaat attggtaaag
    13681 tagaaaaagg aaaagacacc tattaagatg acgctacttg ttataatgga tttagtgttt
    13741 ttcgatatta aaacatacat aagaatagcg ccaattggaa ctgcaactgc agcacgtgta
    13801 ccggagattc cgataccata tataccgcaa atcatgataa atgcaaaaaa aactttcatc
    13861 catctttttt gaacataaaa cattgcgata ccgaaagcac tggctgccat agccgagtga
    13921 actccataat tggccgcatc agagaaacaa gaaaaataac ggataccgga ccatattata
    13981 tgtgtacggg caccacctaa tgtataaagg aaataaagtt ctttatcggt aaaaccatga
    14041 ttcttttgcc aatagccttt gatggtaact atcaatataa agattcccca aatgaataat
    14101 aaagattcaa tacctttacg gtcacggata gaaatcggta cgagaactgc acatataata
    14161 ggaatgaata aataatgtcc aatactaata ttccaggctt cctgtacgtt attaggattt
    14221 gatatttcca ataaacaata tatgccccat atgaaaaaaa gtaataatat tatattgcga
    14281 gaatgcgacc aatctgtctg acgattacta caggcaatta aagtaataaa agtcatgggc
    14341 actattattg ccaaagtagc tatcccaagc ttcacttcaa tgaatatgct tgccaatatt
    14401 ggcaaaaatt gaagagcgaa tagaacataa aaactacgac tggattgatg tgggtctcta
    14461 ttgtatattt cttcttcgtt attcatcgtc atcgttatcg tcgtcctgat aattttttct
    14521 tgaattcttt ttccaacgga tgaaactttc tgtcagagct aattgtgata aacgatacaa
    14581 ttgtttatgt aagaatgtat atgggggcaa cattcccgta taagtttcaa cttcgtactt
    14641 aggggcccaa ttaagataga gatatggcgc tttgcctaat tgagctttta atttttgtaa
    14701 caacattttg tctatatctt tccagccatg atctgcagac gctattacta aatttaattg
    14761 tgcgtcttgt agtatagata caggtatgtt ggcacgattc aaacttggtt gctcaataat
    14821 taaaatatct tcttcttgtg gtatatacag gtctgttatt gtgtttgcca aagcatattt
    14881 cgaagaatta gaatcaaaat cagtcccgtc actcagcata cgtacatgta gacctatacc
    14941 ttcccaatat gcttttagtc tgttggctaa ataggttttc ccacttccat cttctgtact
    15001 gagcatattc aaaatgtatg gcattccttc tttcttttgc gtaaagaatc gaagaataga
    15061 actactcata tatcgagtag caatgtcttc gcattgttta ctgatgggac tgaacttcat
    15121 aggagcaggg tacgcaccta atacaggact tcctgtttgt tttcgcgtac gaaccgaatc
    15181 tcttagagta cggtcgataa attctatcag taagaaaaag gcaattaacc ctataaaact
    15241 acctacgcca atcatcataa caattttgcg acgattagtc gactctgaat taattgggta
    15301 agcaggagga ttaagtactt ttagaatggc ggctgtcatt tctaagcttt ttttacgcaa
    15361 taatgcatca ttgtagcttt ttagattggt caggtagttt tgttctgtaa agttgataat
    15421 tctttctttt cttttaatag tagtacccac aggagcgaaa tgcaagtatt ttgcatttaa
    15481 ttcattgcgg cttttttgta ctatttttag ttcagctttt gctttttcaa ataataaaag
    15541 ctgatctaac cattgttcta caatagtacc tttgtttaga ccttcctttg tatattgacc
    15601 agctacgtat tggttggaaa tctctgatag atctcgtctg gtttgtaata atttgttttt
    15661 gtattctaag agttgtctac cactgtcctc acttgaggtg gaaattgttt ccatctgagt
    15721 gattttgcct gttagatcag aggcttggtt tattttgtcc actaattgaa cgttgtgaat
    15781 gacccgttta atgttactgt ccatttgctt ttcaagttcg ttcagcattg ctttggaact
    15841 gttgtagttg aatagtacat cttgctctct cagttcgaac tctttgttga tagctgctat
    15901 ttcttttgtt tcatcataat agtttataat acgattagtg acattatatt gtgtaagatc
    15961 gtcttcgtaa tttgtcaatt ctttaccgat tcgttttaat tctgatctaa aatattctat
    16021 aactttgtca gtacctccat aacgtaatgc ctgatattca ttgacgaact ctttcatgag
    16081 aatttcaaca gtgttatatg caataccagg atcaccggat tgatagctta ctcttaataa
    16141 atcgctgctt cctttgcggt ctaccttgat gttttttagg gctttatagc tataataaat
    16201 atgttgaaaa tagaacagcc cataaatgta gttgttcata tccggacgtt catacttttg
    16261 gaagttctcc atcgttttat cttcactact tctatcaata agagagataa gagttttacc
    16321 atccttacta tttttcatat gattgtaagt gaagttgtaa caggcagagg ttatgccttc
    16381 gttgtcttta tttgggtctc ctttgaccaa aacacgtgaa aagagtcgta aagatacacg
    16441 ttttaaagtg ctctcagcct gtattatatt gataaggtta tccatggaat tttgagctaa
    16501 ggcccagtct actttagtat tttcttcgat actatagcca gacacaactc ctgtgtagag
    16561 agttgcctct acattatagc cacccttcat atttcctgtc aagtaatagg caatagcaga
    16621 tatgatgaga gttcctaata gaatccacca tttgacacgg tataatgatc ggaaaagata
    16681 gagtatatag tccataatgt tatgtttatt ttcgtagaat tggtgtacgg gttataactt
    16741 caaggatcat tagacttttg gttaactcaa accggctctt ttcaaaagct tctaaagctg
    16801 ttgattgtct tcctttttct acagataaag tgcttgattc tattgttcca tttgcaaaat
    16861 ctttttcggc aatactgtat tgaacagtag ctaattctaa agcttccgca cgtaatgtca
    16921 aaacacttaa ttgggatgtc gctgttgcgt aaagctcaat aatttctttt ttcatatctt
    16981 cgaatttggc ttcgcgctct aaagctgctg ttttcacatt tagcttctgg cgccttacac
    17041 gtggagcaag gtcgaataac tcgtcaagag gaatattcac ggccgctcct acagtgtatg
    17101 tctgctgttt ggaagtagaa taattatata aaataggtgt tgaaacatcg gtaaatgtat
    17161 agtcgttacc gaactttccc cattgataac ttccccgtat attaaaaaag ctaagtaccg
    17221 accgtctttc ttttgccagc agtttgcgtt cgatctgctc ttttacactg gcaagttcat
    17281 agatcggtcc tcctttggcg ttttcgaata acagatcgag gggaggaagt gaaatgttgg
    17341 tgtaatcttc cgggtttagt tttgagaaat cactggtttc ctgggcattt gcgaaagaaa
    17401 ccggtgaaaa tagaagtagg ggtatactac atagtatgaa taaaactctt ttcat
//
