LOCUS       BSU07818                5026 bp    DNA     linear   BCT 31-MAR-1994
DEFINITION  Bacillus stearothermophilus XL-65-6 PTS regulatory protein (celR')
            gene, partial cds, and cellobiose phosphotransferase system operon
            (celA, celB, celC, and celD) genes, complete cds.
ACCESSION   U07818
VERSION     U07818.1
KEYWORDS    Cellobiose PTS operon.
SOURCE      Geobacillus stearothermophilus
  ORGANISM  Geobacillus stearothermophilus
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Lai,X. and Ingram,L.O.
  TITLE     Cloning and sequencing of a cellobiose phosphotransferase system
            operon from Bacillus stearothermophilus XL-65-6 and functional
            expression in Escherichia coli
  JOURNAL   J. Bacteriol. 175 (20), 6441-6450 (1993)
   PUBMED   8407820
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Lai,X. and Ingram,L.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-1994) Xiaokuang Lai and Lonnie O. Ingram, Dept. of
            Microbiology and Cell Science, University of Florida, 3103 McCarty
            Hall, Gainesville, FL 32611-0330, USA
COMMENT     On Mar 30, 1994 this sequence version replaced gi:433021.
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Geobacillus stearothermophilus"
                     /mol_type="unassigned DNA"
                     /strain="XL-65-6"
                     /db_xref="taxon:1422"
                     /clone_lib="genomic"
                     /note="isolated from rotting wood"
     gene            1..1393
                     /gene="celR'"
     CDS             <1..1393
                     /gene="celR'"
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory protein"
                     /protein_id="AAA17388.1"
                     /translation="PDVLELTEAMVVYAEKKLGRRLAEKVMYALAMHIQTAINRLRAGT
                     IVSHPKLNEVRAAYKQEFAVALDCLQLMEERTNIDFPIDEAGFLTMFFAFHEEQAEERE
                     ERVAIVVVMHGNGVASAMADVVNQLLAAACVHAVDMPLDADPKRIYEQVKAVLQPVASK
                     KGALLLVDMGSLVSFSNFLEKELAVPVRVISAASTPHVLEAARKAMLGYALQEIYEEVK
                     AAAPFYIRGPLWEEEAAEQDMLAIVTACFTGKGSALALKHILETYLQLDERMWRIIPIQ
                     MADAEEARQTLSNVAKHFRIVCIVSHLCLDERIPHFSLEDVLSLRAMKEIQALADVEEI
                     HMHMARELTNHLRHLAPARAIPAIRAALAAIGRELGLEADGRDLVGLVFHLCCLLDRLL
                     SGERTSGDRGAAKACGHEDEDGALYRAVKEGLFPLEQQYGVCIDEDELCHIVHFFRSLQ
                     GKRDG"
     regulatory      1482..1513
                     /regulatory_class="promoter"
                     /note="putative"
     gene            1524..1843
                     /gene="celA"
     regulatory      1524..1531
                     /regulatory_class="ribosome_binding_site"
                     /gene="celA"
                     /note="putative"
     CDS             1541..1843
                     /gene="celA"
                     /note="cellobiose PTS enzyme II'"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P46318|4.A.3.2.2"
                     /protein_id="AAA17389.1"
                     /translation="MNILLICAAGMSTSLLVTKMKEAAKQKGIEANIWAVSADEAKSHL
                     DQADVVLIGPQIRYKLAAFKKEGEARGIPVDVINPADYGRVNGAGVLDFALRLKK"
     gene            1842..3216
                     /gene="celB"
     regulatory      1842..1851
                     /regulatory_class="ribosome_binding_site"
                     /gene="celB"
                     /note="putative"
     CDS             1861..3216
                     /gene="celB"
                     /note="cellobiose PTS enzyme II''"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P46317|4.A.3.2.2"
                     /protein_id="AAA17390.1"
                     /translation="MDRFIRMLEDRVMPVAGKIAEQRHLQAIRDGIILSMPLLIIGSLF
                     LIVGFLPIPGYNEWMAKWFGEHWLDKLLYPVGATFDIMALVVSFGVAYRLAEKYKVDAL
                     SAGAISLAAFLLATPYQVPFTPEGAKETIMVSGGIPVQWVGSKGLFVAMILAIVSTEIY
                     RKIIQKNIVIKLPDGVPPAVARSFVALIPGAAVLVVVWVARLILEMTPFESFHNIVSVL
                     LNKPLSVLGGSVFGAIVAVLLVQLLWSTGLHGAAIVGGVMGPIWLSLMDENRMVFQQNP
                     NAELPNVITQQFFDLWIYIGGSGATLALALTMMFRARSRQLKSLGRLAIAPGIFNINEP
                     ITFGMPIVMNPLLIIPFILVPVVLVVVSYAAMATGLVAKPSGVAVPWTTPIVISGYLAT
                     GGKISGSILQIVNFFIAFAIYYPFFSIWDKQKAAEEQADPTISSGAGATHSL"
     gene            3215..3968
                     /gene="celC"
     regulatory      3215..3221
                     /regulatory_class="ribosome_binding_site"
                     /gene="celC"
                     /note="putative"
     CDS             3231..3968
                     /gene="celC"
                     /note="putative phospho-beta-glucosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="CE0"
                     /protein_id="AAA17391.1"
                     /translation="MPRYCIVNADDFGYSKGVNYGILEAFQNGVVTSATLMANMPAAEH
                     AARLAKDHPELGVGIHFVLTCGRPLADVPSLVNENGEFPRRGEALVGARRGDIERELCA
                     QLERFFSFGLTPTHIDSHHHVHEHPNVFPVVEQLAERYRLPIRPVRTARPHRLPTVDVF
                     FPDFYGDGLTKDRFISLIDRIGDGQTAEVMCHPAYIDVPLASGSSYCQQRVEELAVLTD
                     PTLVAEMAERGVQLITYREFYKL"
     gene            3967..4306
                     /gene="celD"
     regulatory      3967..3975
                     /regulatory_class="ribosome_binding_site"
                     /gene="celD"
                     /note="putative"
     CDS             3980..4306
                     /gene="celD"
                     /note="cellobiose PTS enzyme III"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P46319|4.A.3.2.2"
                     /protein_id="AAA17392.1"
                     /translation="MQTYEQTVFQLILHGGNGRSYAMEAITAAKKGEFAEARRLLEQAG
                     AELQAAHGLQTALLQQEASGGQPVVTLLMVHAQDHLMTAITVKDLAAEFVELYEALKRQ
                     TTES"
ORIGIN
        1 tcccgacgtc cttgagttga ccgaagcgat ggtcgtctat gcggagaaaa aacttgggcg
       61 ccgcctcgcc gagaaggtga tgtatgcgct cgccatgcac attcaaacgg cgatcaaccg
      121 cctgcgggcc gggacgatcg tttcccatcc gaaactgaat gaagttcgcg ccgcctataa
      181 gcaggaattc gccgtcgcgc tcgactgcct tcagctgatg gaagaaagga cgaatatcga
      241 cttcccgatc gatgaagcgg gatttttgac gatgtttttt gcgttccacg aggagcaagc
      301 ggaagagcgg gaggaacggg tggcgatcgt ggttgtgatg catggcaacg gtgtggcttc
      361 ggcgatggcg gacgtcgtga atcagctgtt ggccgccgcg tgtgtgcatg cggtcgatat
      421 gccgcttgat gccgacccga aacgaattta cgagcaggtg aaagcggtgc tacagcccgt
      481 cgcatcgaaa aaaggggcgc tgttgcttgt tgacatggga tcgctcgtgt cgttttcgaa
      541 ctttttggaa aaagagctcg ctgttccggt gcgggtgatt tccgctgcca gcacgccgca
      601 cgtgctcgaa gcggcccgca aagcgatgct cggctatgcg cttcaggaaa tttacgaaga
      661 agtgaaagcc gcagcgccgt tttacattcg cgggccgctt tgggaggagg aggctgcgga
      721 gcaggacatg ctggcgatcg tcaccgcctg ttttactgga aaaggaagcg cgctggcgct
      781 aaagcatata ttggaaacgt atttgcagct cgatgagcgc atgtggcgca ttattcccat
      841 tcaaatggca gatgcggaag aagcgcggca aaccttgtcc aacgtcgcga aacatttccg
      901 catcgtctgt atcgtcagcc atctttgcct tgatgagcga atcccgcact tttctcttga
      961 ggacgtgctt agcttgaggg cgatgaaaga aatccaagcg ttagccgacg ttgaggaaat
     1021 tcatatgcat atggctagag agctgaccaa tcatcttcgc cacctcgcac cggcgcgggc
     1081 cattcccgcc attcgcgctg cgctggcggc gattggtcgg gaactcggcc ttgaagcgga
     1141 tggccgcgat ctggtggggc ttgtctttca tctttgctgc ttgctcgatc gcctgctgtc
     1201 cggagagaga acgagcggtg accgaggagc agcgaaagct tgcgggcatg aagatgaaga
     1261 tggcgcactg tacagggcgg tcaaggaggg gttgtttccg cttgaacagc agtacggtgt
     1321 ctgcatcgat gaagatgaac tgtgtcatat cgttcacttt tttcgctccc tgcaaggaaa
     1381 acgagatgga taaggtgcga taattggaaa cgcttccatt acacagtttc cctccgttaa
     1441 cacacaaccg ttccatccgc ttcagcgctg tcttttcggc gttggcatgg aatttgcttg
     1501 aacaataatg aatcattgca aaaaaaggag gaggaagcgg atgaacattt tgctcatttg
     1561 cgctgccggc atgtcgacga gtttgcttgt gacgaagatg aaggaggcgg caaagcaaaa
     1621 agggatcgag gcgaacattt gggctgtgtc agctgatgaa gcgaaaagtc atctcgacca
     1681 ggcggatgtt gtgctgattg gtccgcaaat ccgttataag ctcgccgcct tcaaaaaaga
     1741 gggagaggcg cgcggcattc cggttgacgt catcaatcca gctgactatg ggcgggtcaa
     1801 cggcgccggc gtgttggact ttgcgctgcg gttaaaaaaa taaacagggg gtagaagcgg
     1861 atggaccggt ttattcggat gttggaagac cgcgtgatgc ctgtcgccgg caagattgcc
     1921 gaacagcgcc atttgcaggc gattcgtgac gggattattt tgtcgatgcc attgttgatt
     1981 atcgggtctt tatttttaat cgttggcttt ttgccgattc ccggttacaa cgaatggatg
     2041 gcgaaatggt tcggcgagca ttggcttgat aagcttctct atccggttgg agcgacgttc
     2101 gatattatgg cgcttgttgt cagcttcgga gtcgcctatc ggctggcgga aaagtataaa
     2161 gtggatgcgc tgtccgccgg cgcgatttca ctggctgctt ttttgctcgc aaccccgtat
     2221 caagtgccgt tcacgccgga aggagcgaaa gaaaccatta tggtcagcgg tggcatcccg
     2281 gtgcaatggg tcggcagcaa ggggttgttt gttgccatga ttttggcgat tgtgtcaacc
     2341 gaaatttacc ggaaaatcat tcaaaaaaat attgtcatta agctgccgga cggggtgccg
     2401 cctgctgtgg cccgctcctt tgttgctttg atcccgggag ccgccgttct cgtcgttgtc
     2461 tgggtagccc ggctgatttt ggaaatgaca ccgtttgaaa gtttccataa cattgtatct
     2521 gtcctcctga acaaaccgct cagtgtgctc ggcggcagtg tatttggcgc cattgtcgcc
     2581 gtgctgcttg tccagctgct atggtcgacc ggactgcacg gagcggccat cgtcggcggg
     2641 gtcatggggc cgatctggct gtcgctgatg gatgaaaatc gaatggtgtt ccagcaaaat
     2701 ccgaatgccg aactgcccaa cgtcattacg caacagtttt ttgatctttg gatttacatc
     2761 ggcggttcag gagcgacatt ggcgttggcg ttgacgatga tgtttcgggc ccgcagccgg
     2821 cagctgaaaa gcttaggtcg gctggcgatc gcgcccggca ttttcaatat taatgagccg
     2881 atcacgttcg gcatgccgat cgtcatgaac ccattgctga tcattccgtt cattctcgtg
     2941 cctgtcgtgc ttgtggttgt ttcctacgcc gcgatggcga ccgggcttgt cgccaaacca
     3001 agcggggtgg ccgtgccatg gacgacaccg atcgtgatca gcggctattt agcgacggga
     3061 ggcaaaattt ccgggagtat tttgcaaatc gttaacttct tcatcgcgtt tgccatctac
     3121 tatccatttt tctcgatttg ggacaaacaa aaagcggccg aagagcaggc cgatccaacg
     3181 atttcaagcg gagcgggggc aacgcactcg ctgtaaagga gaggaacgaa atgccgcgct
     3241 attgcatcgt caacgccgat gatttcggtt actcgaaagg ggtcaactac gggattttgg
     3301 aagcgtttca gaacggtgtc gtcacgtcgg cgacgctgat ggcgaatatg ccagcggccg
     3361 aacacgccgc ccggctggcg aaggaccatc cggaactcgg cgttggcatt cattttgtgc
     3421 tgacgtgcgg ccggccgctg gccgatgttc catcgctggt gaacgagaat ggggagtttc
     3481 cgcggcgcgg ggaggcgctt gtcggcgcta ggcgcggcga tatcgagcgg gagctttgcg
     3541 cccaattgga gcgttttttc tcgttcgggc tcactccgac gcatattgac agccatcatc
     3601 acgttcatga gcacccgaat gtgtttccgg ttgtggaaca attggccgaa cgctatcggc
     3661 tgccgatccg cccggtgcgg acggcacggc cgcatcggct gccgaccgtc gacgtctttt
     3721 ttccggattt ttacggcgat ggactgacga aagaccgttt tatctcgctg atcgaccgaa
     3781 ttggcgacgg gcagacggcg gaagtgatgt gccacccggc gtatatcgat gttccgctcg
     3841 cgtcaggaag ttcctattgt caacaacgag tcgaagagct ggcggtgctg accgacccaa
     3901 cgctcgttgc cgagatggcc gagcgcggtg ttcagctgat cacgtaccgc gaattctata
     3961 aactatagga gagggcgtta tgcaaacgta tgaacaaact gtattccaac tgattcttca
     4021 tggcggaaac ggccgcagtt atgcaatgga ggcgattacg gcggcgaaaa aaggggaatt
     4081 tgccgaggcg cgcaggctgc ttgaacaggc gggagcagaa ctgcaggcgg cccatgggct
     4141 gcagaccgcg ttgctgcaac aagaagcgag cggcgggcag ccggtggtga cgcttctgat
     4201 ggtgcatgcc caagatcatt taatgacggc gatcacggtc aaggatttag ccgctgaatt
     4261 cgtggagctg tatgaagcgc tcaagcggca aacaaccgaa tcataattgt cgtcggccgg
     4321 cttgttgacc gggtgaggat gattcgcttc aacgggaatg aacccaaggc gggcggcaac
     4381 gggctgttct agcgaccgcc cgctttgttg attttacaat aattttatta tgtgaacata
     4441 agggcgccag ccgttcagcc ctcccggctg ccgccggtct cggctgaacg gagaaggaaa
     4501 actccgcttg tttgcgtcac atatcgtggc ttgtgttgtg cttttgccgc gtgtgattgc
     4561 ggttcttcac ttttttcgaa ccgtcaagcg gctccggctg cccaggattg tcgccttttg
     4621 gggcgttttt gcgcatatct ttgccgtcgt ttttgttcgt catttgttgt ccctcctttg
     4681 tccgttagca tgcggcgttg tgcttttgct tattcggggc ataatttgcg ccggtttgaa
     4741 acgataagta gtggatgacc gcgatgactg cgaaggagga acgagccatg gtttatcata
     4801 aaacgaagca agacgcgttt caggcgcggc aaaaggcgac gatggaggca aaagagtggc
     4861 atgaccatct cgtccgcgat caagccgatt acggccatca gttagcccat ttgcggcagg
     4921 aagtgaacga agcgtttgca caaattgaaa acgcgcttga agtcgcctcg gaaacgcagc
     4981 gcatgcagct tgagaaattc cgcagcgatt tgcaggcgat ccccgg
//
