LOCUS       NZ_DS499729             8289 bp    DNA     linear   CON 25-APR-2021
DEFINITION  Anaerostipes caccae L1-92 strain DSM 14662 Scfld_03_10, whole
            genome shotgun sequence.
ACCESSION   NZ_DS499729 REGION: 73694..81982
VERSION     NZ_DS499729.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00627053
            Assembly: GCF_000154305.1
KEYWORDS    WGS; RefSeq.
SOURCE      Anaerostipes caccae L1-92
  ORGANISM  Anaerostipes caccae L1-92
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae;
            Anaerostipes.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Anaerostipes caccae (DSM 14662)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Anaerostipes caccae (DSM 14662)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8289)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   4  (bases 1 to 8289)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   5  (bases 1 to 8289)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            DS499729.1.
            Anaerostipes caccae (GenBank Accession Number: AJ270487) is a
            member of the division Firmicutes. It is an acetate-converting
            butyrate-producing colon bacteria that is involved in metabolic
            cross-feeding with Bifidobacterium species (Falony et. al. (2006),
            Belenguer et. al. (2006)). The sequenced strain was obtained from
            Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ)
            (DSM 14662).
            
            We have collected 9.8X coverage in plasmid end reads (pOTW13 and
            pJAZZ vectors) and 454 reads. We have performed one round of
            automated sequence improvement(pre-finishing), along with manual
            improvement that includes breaking apart any mis-assembly, and
            making manual joins where possible. Manual edits also are made
            where the consensus appears to be incorrect. All low quality data
            on the ends of contigs is removed. Contigs are ordered and oriented
            where possible.
            
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences.  454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs. These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads.  Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Anaerostipes caccae (GenBank Accession Number: AJ270487) is a
            member of the division Firmicutes. It is an acetate-converting
            butyrate-producing colon bacteria that is involved in metabolic
            cross-feeding with Bifidobacterium species (Falony et. al. (2006),
            Belenguer et. al. (2006)). The sequenced strain was obtained from
            Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ)
            (DSM 14662).
            
            We have collected 9.8X coverage in plasmid end reads (pOTW13 and
            pJAZZ vectors) and 454 reads. We have performed one round of
            automated sequence improvement(pre-finishing), along with manual
            improvement that includes breaking apart any mis-assembly, and
            making manual joins where possible. Manual edits also are made
            where the consensus appears to be incorrect. All low quality data
            on the ends of contigs is removed. Contigs are ordered and oriented
            where possible.
            
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences.  454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs. These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads.  Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community.
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were
            blasted against NCBI's non-redundant (NR) database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.0. Gene names are generated at the contig level and may not
            necessarily reflect any known order or orientation between contigs.
            This information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            
            Annotation was added to the contigs in February 2008.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Product names were updated in August 2012.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 04/25/2021 09:13:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 3,533
            CDSs (total)                      :: 3,441
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 92
            rRNAs                             :: 5, 6, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 72 of 99
            Pseudo Genes (incomplete)         :: 23 of 99
            Pseudo Genes (internal stop)      :: 12 of 99
            Pseudo Genes (multiple problems)  :: 8 of 99
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Anaerostipes caccae L1-92"
                     /mol_type="genomic DNA"
                     /strain="DSM 14662"
                     /isolation_source="biological product [ENVO:02000043]"
                     /host="Homo sapiens"
                     /type_material="type strain of Anaerostipes caccae"
                     /db_xref="taxon:411490"
     gene            complement(<1..8)
                     /locus_tag="ANACAC_RS03030"
                     /old_locus_tag="ANACAC_00492"
     CDS             complement(<1..8)
                     /locus_tag="ANACAC_RS03030"
                     /old_locus_tag="ANACAC_00492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="WP_006566000.1"
                     /translation="MSKEEFLGSLNRLLKSLGKSEREKSLSYYNEIIDDYMEDGYTEE
                     QAVEQIGNPGLIAQEILEEQQSQMKAPMSRGTKVLVAVLLVLGFPLWGSLMLAGFCIV
                     LSAVLLVLSAYIVIWCIPLCTGAASVAGLILSVVSMGGAAVIVFQNSAAGVIQLGVGM
                     LSAGIFILTGLLTWVLGKYFVKVTANFSKWLAGTVTRIRRKNV"
     gene            complement(1..318)
                     /locus_tag="ANACAC_RS03035"
                     /old_locus_tag="ANACAC_00493"
     CDS             complement(1..318)
                     /locus_tag="ANACAC_RS03035"
                     /old_locus_tag="ANACAC_00493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_006566001.1"
                     /translation="MNTQLKKGFLEFCILAALCEDDSYGYQIIKDVSGCIKISESTLY
                     PILKRLESSGYLDTYTVEHNSRLRKYYRITEKGRDHIREFLDEWQQIMGIYEFVKGAA
                     EHE"
     gene            complement(477..1448)
                     /locus_tag="ANACAC_RS03040"
                     /old_locus_tag="ANACAC_00494"
     CDS             complement(477..1448)
                     /locus_tag="ANACAC_RS03040"
                     /old_locus_tag="ANACAC_00494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="WP_006566002.1"
                     /translation="MTRKLCGIGEALIDFIPEVKGQRLKDVPSFTRVAGGAPANVAGA
                     VTKLGIPSKFLTKLGDDPFGDYIVEVLDEAGIDTSHIKRDKEAETALAFVSLASDGNR
                     DFKFYRKNSADLRYSVYDIEPDVLDDCGMIHFCSVDLVNSPMKDAHKKLIDMANGKDV
                     LVSFDPNLRFSLWDDLQELKETVNEFLPFADIIKISDEELEFITGHTDIREAVPDLLN
                     GRTKYVIYTKGKDGAEIYTKHGMTEAPGYSIDVRDTTGAGDSFIGAFLYQLLRDNVTD
                     LEAVNFGTLKEYLDFANAYAAYTTTREGALAAMADAREFADFRKNLQ"
     gene            complement(1445..2908)
                     /locus_tag="ANACAC_RS03045"
                     /old_locus_tag="ANACAC_00495"
     CDS             complement(1445..2908)
                     /locus_tag="ANACAC_RS03045"
                     /old_locus_tag="ANACAC_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_006566003.1"
                     /translation="MFKKYNKVLKEDYEKWYAEHESDREETAKDPMRLKFHLMPKTGW
                     LNDPNGLCQFHGTYHIYYQYTPFEPTGELKLWGHYTTKDFISYIDEGPVLFPDSEFDA
                     HGVYSGSAFVKGDTIHYFYTGNIKCFDRDDYDYINSGRGSNTIHVASKDGYHFSEKDL
                     LMTTDDYPEDMSCHVRDPKIIRRGDDYYMVQGARDIDSHGLILMFHSKDLSHWEYFDR
                     IVPKEPFGYMWECPDLFELDGQMMLITCPQGVEPKGIDYQNVHQCTAVKMDYDFENKS
                     YEICDNSEFQQLDRGFDFYAPQTFEDEKGRRILIGWMGIPDADYTNPTVEHGWQHALT
                     IPRELHIKDGRIIQRPIKEMEQMRKGELICTFSEQGRMESNLKLYEAEVRFRHCADMV
                     LTLKEGVTLSYDSGLLTLDLGDFGEGRTTRCAALDELKDLRIFADTTSLEIFVNGGEE
                     VFTTRVYSSQGKLSIDGDCSGTMKIYCLRPLEIGERE"
     gene            complement(2919..4832)
                     /locus_tag="ANACAC_RS03050"
                     /old_locus_tag="ANACAC_00496"
     CDS             complement(2919..4832)
                     /locus_tag="ANACAC_RS03050"
                     /old_locus_tag="ANACAC_00496"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="WP_006566004.1"
                     /translation="MDYAKIASQVIANVGGKENIKSVAHCATRLRFQLKNNDLRNEEA
                     ISDLDGVKGVFLTQSQFQIIFGSGLVNLICDEVQKQIGDVSSAAGEEEKEEKQGSVVQ
                     RFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAGLFDGRSIIELYPQWKGLTQAVNTFA
                     NAPFTFLPVLIGFSATKKFGGNPFLGAAMGMIMVHPDLLNAYQIGIGQAPVWDIFGFN
                     IQAIGYQGTVLPVLAISWILAQIEKRLHKVTPTWLDNLTTPLLSIIITAFLTFICVGP
                     VLREAGNLLADGITWVYNTLGAVGGGLFGLVYAPITMTGLHHSFIAIETQLLAAKAVT
                     GGSFIFTTASMNNVAQGAAVLAVLFLTKDQKQKSICSASGVSALLGITEPAMFGVTLK
                     LKYPFYAAIIGSAVGSAYAAATKTLAQALGAAGLPGFISMKPDHFLHFGIGLILSMVI
                     SAGLTVVFWKKYNLGGNEKTGKPVQSSEETAEIPEQAETAETVLYSPMNGEVLPITES
                     EDEAFASKAMGDGVAVNPSEGTVYAPCDGTITMIFPTKHAIAIQAETGKSFLIHAGID
                     TVKMEGEGFETFAEAGTKVKQGDKILTFDIDLVKEKGYSPQTMMMLAEGDGQVEVIPK
                     TDADTKTQVMIVR"
     gene            complement(5003..6037)
                     /locus_tag="ANACAC_RS03055"
                     /old_locus_tag="ANACAC_00497"
     CDS             complement(5003..6037)
                     /locus_tag="ANACAC_RS03055"
                     /old_locus_tag="ANACAC_00497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006566005.1"
                     /translation="MNIYRILNKIKTKIPESERDLEDQEMTDQMKTMARKIAGKQKRR
                     QAFFKKAAAAAIVVIIGGGTAVAASGGWGNLIPKLFNSTLIGKGTETDLEEYSGSDKV
                     MAEAESKNIKVKVVQSAASKGMIYILLKTELPENQTFKEDQIFKYSLLKTGAKDDPKK
                     KEMTGMNADRLLLVQKKENIGWFILQAERSGHDFKNQKIMLSLEGFTSTHTTAEDLIF
                     ENPKGSWKLTWKVENSKMETAEVHVDKEYHFQNGKEIIKKIEITPFYFRVYHGKKGSS
                     EDNSNLPECSLRIKDGTVKKLHWSYSQGADRSANGYAFHQSDFTELINPDNVEAVIIG
                     GNEISVSKNK"
     gene            complement(6034..6588)
                     /locus_tag="ANACAC_RS03060"
                     /old_locus_tag="ANACAC_00498"
     CDS             complement(6034..6588)
                     /locus_tag="ANACAC_RS03060"
                     /old_locus_tag="ANACAC_00498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_039946252.1"
                     /translation="MKATEKNFINLIRKRREEGLLYAVETYGWVIQSVVRKHLFLLPD
                     LQQECMNDVLMAVWQHIGDFDPNTGSFQNWAAGIARFKAIDCKRNYLRRSRKYVDIED
                     QFIEDEHAGRQMLKVELDQEIKELFEQLSQPEREIFQKHFIEEATIEEISAEMGLNES
                     TIYQRISRGRKKLRKHKEIREAGQ"
     gene            complement(6716..7705)
                     /locus_tag="ANACAC_RS03065"
                     /old_locus_tag="ANACAC_00499"
     CDS             complement(6716..7705)
                     /locus_tag="ANACAC_RS03065"
                     /old_locus_tag="ANACAC_00499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_182483054.1"
                     /translation="MPKKITMADVAEMAGVTKSTVSRYFNGGYVKKATREKIQKVIEE
                     YNYAPNTFARLKAKESNVIGVVVPTLNSKVTSRVITSIDRYLRDRNYTTLIKNSDHDI
                     DLELLNIQKLIHLNVDGILISSIAVTEDHRDIFEQTQIPIVVLAQDYEDGISIIDDDY
                     NAGKCVGEYIGKTGHKNVGYIGVYETDEAVGIRRRQGVLDGLAAFGIHDPLIKKSDYS
                     FLGGQKNTREILEEMKPDAIVCATDRLAFGAYKVLQEKGLRIPEDISVVGFGGYDESS
                     LLTPELTTLKFDSYGMGYLGAETILKMIKEEPVSKKQIVEYKFIEGKSVKNRT"
     gene            7846..8289
                     /locus_tag="ANACAC_RS03070"
                     /old_locus_tag="ANACAC_00500"
     CDS             7846..8289
                     /locus_tag="ANACAC_RS03070"
                     /old_locus_tag="ANACAC_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_006566008.1"
                     /translation="MKLEDYDSRHLLFAALFMTSNRLQSIGDRFYEEITSKQWFLLAA
                     LNTFEYAPTFNELSSLMGSSHQNVKQIALKLQQKGYAEIETDPDDRRKSRVRLGRKAE
                     SLQSAYALKEEAFMDLLYKDISETQLKTALETLNTMMKNMEECNI"
     gene            8286..>8289
                     /locus_tag="ANACAC_RS03075"
                     /old_locus_tag="ANACAC_00501"
     CDS             8286..>8289
                     /locus_tag="ANACAC_RS03075"
                     /old_locus_tag="ANACAC_00501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="WP_006566009.1"
                     /translation="MKKTAVVYGSVYGSTKQYAQWIADSLSADLYENKNLDLSVLEPY
                     DVIIYGGGLYAGGVTGISLISRHPEFIQKKKVFVFTCGIADPALPENVEHILKNLDSV
                     FPGELKQQISFYHLRGWMDYSRFSRKHRVMMWMMKKMLSKKKLEDLTEEDQGILETYG
                     KHVDFTDRDSILPLVQDVRQYI"
ORIGIN      
        1 ttactcatgt tctgccgctc ctttcacaaa ctcataaata cccatgatct gctgccactc
       61 atccaaaaat tctctgatat ggtctcttcc cttttctgtt atcctgtaat actttctgag
      121 acggctgttg tgttccaccg tgtaagtatc cagatatccg ctgctttcca gacgtttcag
      181 aatcggatac agcgttgact cggaaatctt aatacatccc gatacatctt tgatgatctg
      241 gtaaccatag gagtcatcct cacagagggc agccagaata caaaactcta aaaaaccttt
      301 tttcaactgt gtattcaatc tatcgcctcc ttatgcatta tactatatat tacatagtat
      361 aatgtgtcaa gatgtatttt caatttcatc ctagatctat tcgtaaaaaa tagaaaaaag
      421 ccagcgaatc cttttttcaa agactcattg gcttttcacg ttttatatat tttaatttac
      481 tgcaggttct ttctgaaatc ggcaaattcc ctggcgtctg ccatggctgc cagcgctcct
      541 tccctggttg tagtataagc cgcataggcg ttggcaaaat ccagatactc ctttaaagtc
      601 ccgaaattca ccgcttccag atccgtcaca ttatccctca gaagctggta taaaaatgct
      661 ccgataaagg aatcaccggc tccggtcgta tctcttacgt caatggaata tcccggtgct
      721 tccgtcattc catgtttcgt atagatctcc gcaccgtctt ttccttttgt atagatgaca
      781 tacttcgtcc ttccgttcag caggtcaggc actgcctccc ttatatccgt atgtcctgtg
      841 ataaactcca gttcttcatc tgagattttt ataatatccg caaaaggcag aaattcattg
      901 actgtctctt tcagttcctg taagtcatcc cacagtgaaa aacggaggtt tggatcaaat
      961 gatacaagca cgtctttgcc gtttgccatg tcaattaatt tcttatgtgc atctttcatg
     1021 ggtgaattca ccaagtctac gctgcagaaa tggatcatcc cgcagtcatc cagaacatcc
     1081 ggctcaatgt catagacact gtagcggaga tctgcgctgt ttttgcggta aaacttaaaa
     1141 tccctgtttc cgtctgatgc cagagagaca aaggccagag ccgtctctgc ttctttgtcc
     1201 cgtttaatat gacttgtgtc aattccagcc tcatcaagta cttcaactat ataatcaccg
     1261 aagggatcgt ctccaagttt tgtcaaaaac ttggacggaa tcccaagttt cgtcaccgca
     1321 ccggctacat tggcaggagc ccctcctgct actctggtaa aggacggtac atcctttaat
     1381 ctctgtcctt tcacttccgg aataaaatca atcagtgcct ctccgattcc gcataatttt
     1441 ctggtcattc tctttctcct atctccagag gccgcaggca atagatcttc atggtacccg
     1501 aacagtcccc gtctatgcta agctttccct ggctgctgta taccctggtg gtaaagactt
     1561 cttcaccgcc gtttacaaaa atttcaagag aagtagtgtc tgcgaaaatc cgcaggtctt
     1621 ttaattcatc caatgcggca catcttgtcg tcctgccctc accaaaatcc cccagatcaa
     1681 gggtgagcag tccgctgtca taggataagg taacgccttc ctttaacgtc agaaccatat
     1741 cagcgcaatg tctgaatcga acctctgctt catacagttt cagattgctc tccatcctgc
     1801 cctgttcaga aaatgtacag atcagttctc cttttctcat ctgttccatc tcctttatag
     1861 gccgctgaat gatccttcca tcctttatgt gcagttccct tgggattgtc agtgcgtgct
     1921 gccatccatg ctcaacggtc ggattcgtgt aatcggcatc cggaatcccc atccatccga
     1981 tcaggatacg gcgtcccttc tcgtcctcaa aggtctgggg tgcgtaaaaa tcaaagcccc
     2041 tgtctaactg ctgaaactct gaattatcgc agatctcata tgatttgttc tcaaagtcat
     2101 aatccatctt caccgctgta cactgatgaa cattctgata gtcaatgcct ttcggttcta
     2161 ccccctgagg gcatgtgatc agcatcatct gtccgtccag ttcgaacaag tccggacact
     2221 cccacatata accgaacggt tctttcggaa cgatccggtc aaaatactcc caatgagaca
     2281 gatcctttga atgaaacatc aggatcagtc catgactgtc tatatccctg gctccctgga
     2341 ccatatagta atcatccccc cgccggatga tcttcgggtc ccgcacatgg cagctcatat
     2401 cctcaggata atcgtccgtg gtcattaaaa ggtctttttc agaaaaatga tatccgtctt
     2461 tgctggccac atgaatcgtg ttgctgcccc gccctgagtt gatgtaatcg taatcatccc
     2521 tgtcaaaaca cttgatattc cctgtataga aatagtgaat cgtatctcct tttacaaagg
     2581 cggaaccgga gtagacgccg tgagcgtcaa actccgaatc cggaaataat accggtcctt
     2641 catctatata agaaataaaa tcttttgtcg tataatgccc ccaaagcttc agttctccgg
     2701 tcggttcaaa cggggtgtac tgataataga tatggtaggt gccgtgaaac tggcacagtc
     2761 cgttgggatc gttgagccat ccggtcttag gcatcaggtg aaatttaagc cgcatcggat
     2821 ctttcgctgt ctcttctctg tcactttcat gttcagcata ccacttttca tagtcttctt
     2881 tcaatacttt attatacttc ttaaacatga tttccctctt atctaacgat cataacctgt
     2941 gttttcgtat ctgcatccgt ctttggaatg acttccacct gtccgtctcc ctctgccagc
     3001 atcatcatag tctgaggact gtaacccttt tctttcacga gatctatatc aaacgtaagg
     3061 atcttatctc cctgttttac cttcgttccg gcttctgcga acgtctcaaa gccttcccct
     3121 tccatcttta cggtgtcaat tcccgcatga atcagaaaac tctttcccgt ttctgcctga
     3181 atcgcaatcg catgttttgt cggaaagatc atcgttatcg taccgtcaca cggcgcatac
     3241 actgtgccct cagaaggatt gacggccacg ccgtctccca tggctttcga tgcgaatgct
     3301 tcatcttcgg actccgtgat gggaagcact tccccattca tcggggagta aagcactgtt
     3361 tccgctgttt ctgcctgttc cggaatttct gctgtttcct cagaagactg taccggcttt
     3421 cctgtttttt catttccgcc aaggttatac tttttccaga acacaacggt gagccccgct
     3481 gaaatcacca tggagaggat caatccgatt ccaaagtgaa gaaaatgatc cggtttcatg
     3541 gatataaatc cgggaagtcc cgctgctccc aatgcctgtg ccagtgtttt ggtagctgct
     3601 gcgtatgcac ttcccactgc ggaaccaatg atcgccgcgt agaaaggata tttcaatttc
     3661 agtgtcacgc caaacatcgc cggctccgta attcccaata atgcggagac tccggatgca
     3721 gaacagatag acttctgttt ctggtctttt gtgaggaaga ggactgctaa aactgccgct
     3781 ccctgtgcaa cattgttcat acttgccgtt gtaaagatga agcttcctcc ggtcactgcc
     3841 tttgcggcca gaagctgagt ttcgatggca ataaagctgt ggtgaagccc tgtcattgtg
     3901 atcggtgcgt atacaaggcc aaagagtccg cctcccacag cgccgagagt attgtaaacc
     3961 catgtgatgc cgtctgctaa taagttgcct gcttctctta ataccggtcc cacacagatg
     4021 aatgttaaaa atgcagttat aatgatagaa agcaatggtg tcgtaagatt gtcaagccag
     4081 gtaggtgtta ctttgtgaag ccttttttca atctgagcca gaatccagga aattgccagt
     4141 acaggaagta ctgttccctg ataaccgatc gcctgaatgt tgaatccgaa gatatcccac
     4201 accggtgcct gcccgatgcc gatctgatag gcatttaaaa gatccgggtg aaccatgatc
     4261 atacccatgg ccgcacctaa aaacggattg cctccaaact tcttcgtggc gctgaatccg
     4321 atcagtactg ggagaaaggt aaacggtgcg ttggcgaatg tattgaccgc ctgagtcaac
     4381 cccttccact ggggatataa ttcaatgatc gatctgccgt cgaataatcc cgccgtcaga
     4441 atattattaa gtcccatcag aagaccgcct gccacaatgg ccgggataat cgggacaaaa
     4501 atatcactca gcatctttac aaaacgctgt acaacagagc cctgtttttc ttctttctcc
     4561 tcttctcctg cggctgagga aacatccccg atctgtttct gtacttcgtc acagatgaga
     4621 tttacgagtc ctgatccaaa aataatctgg aactgtgact gggttaaaaa gacacctttt
     4681 accccatcca ggtcactgat ggcctcttca tttctcaaat cattattctt cagctgaaaa
     4741 cgcagcctgg tcgcacagtg cgccacagat ttgatattct cttttccgcc tacgttagcg
     4801 atgacctggc ttgcaatctt tgcataatcc atagttttct cttatcctcc ttacgatatt
     4861 tgtggtatcg gttccatatt tgtattatag gacgattctt tctctacgtc aacacttttc
     4921 tggtatcggt tccataaaaa attgcacaaa atagaagact cctgtttggg agtcttctac
     4981 aaagtcttaa tctatttatc gattatttat tttttgaaac cgatatttca ttgcctccta
     5041 taatgaccgc ctctacgttg tcaggattta tcagctctgt aaaatcactt tgatgaaaag
     5101 catatccgtt tgccgacctg tcagccccct gactgtaaga ccagtgaagc tttttgaccg
     5161 ttccatcctt gatccttaaa gaacattccg gtaaattgga attatcctcc gaactgccct
     5221 ttttgccatg ataaaccctg aaataaaatg gagtaatctc tatcttcttt atgatttcct
     5281 ttccgttttg gaaatgatat tctttatcca catgaacctc tgccgtttcc attttgctgt
     5341 tttccacttt ccaggtcagc ttccatgagc ctttgggatt ctcaaaaatc aaatcttctg
     5401 ccgttgtatg ggtagatgta aatccctcca gtgacaacat gatcttctga tttttaaaat
     5461 catgaccaga tctctctgcc tgtaatataa accatccgat attctctttt ttctgaacca
     5521 acagcagacg gtccgcgttc atccctgtca tttctttctt ctttgggtca tcttttgctc
     5581 cggtctttaa aagactgtat ttgaaaatct gatcttcctt aaatgtctgg ttctccggaa
     5641 gttctgtttt cagcagtata taaatcatac ctttggaggc tgccgactgt accactttca
     5701 ctttaatatt tttactttca gcctctgcca ttactttgtc acttccgctg tactcctcca
     5761 gatctgtctc tgtgcctttt ccgatgagcg tgctgttaaa taatttgggt atcagatttc
     5821 cccagcctcc ggaagctgcc acggcagttc ctcctccaat aatcacaaca atggccgctg
     5881 ccgctgcctt cttaaaaaaa gcctgccgcc gtttctgttt ccctgcaatc tttcttgcca
     5941 tcgtcttcat ttggtctgtc atttcctgat cttccagatc tctctcgctc tccggaatct
     6001 ttgtttttat cttatttaag atgcgatata tattcattgt cccgcctccc tgatctcttt
     6061 atgttttctg agcttttttc ttcctcttga tattctctga tagatcgtac tttcatttaa
     6121 tcccatttcc gctgatatct cttcgatggt ggcttcctca ataaaatgct tctgaaaaat
     6181 ttcacgctcc ggctgggaca gctgttcaaa taattctttt atttcttggt ccaattctac
     6241 cttcagcatc tgtcttccgg catgttcatc ttcaatgaac tggtcttcta tatccacata
     6301 ttttctgctc cgtctcaaat agttccgttt acaatcgatt gccttaaatc tggcaattcc
     6361 cgctgcccag ttttgaaaac tgcctgtatt cggatcaaaa tcaccgatgt gctgccagac
     6421 tgccatcaaa acatcattca tacactcctg ctgcagatcc ggcagcaaaa acaaatgctt
     6481 cctaaccaca gactgaatga cccatccgta ggtttccacg gcatagagca gcccttcctc
     6541 cctccttttc ctgatcagat ttataaaatt tttctccgtt gctttcataa ctttgtctcc
     6601 tgtcccctga acagttcccg ccttcactct atatttcgta tacagacaga catttctgac
     6661 agaaaaaaag tcataaaatc cagcttaatc cggatcttat ggcctttttg cagtcctatg
     6721 ttcgattttt tacgctctta ccttctataa atttatactc tacgatttgt ttcttggata
     6781 ccggttcttc ttttatcatc ttgagaatcg tctccgcccc gagatatccc attccatagg
     6841 aatcaaattt cagtgtcgta agctccggcg ttaaaagact gctctcgtca tatccgccaa
     6901 atcccaccac cgaaatatct tccgggatgc gaagcccttt ctcctgcaaa actttatagg
     6961 ctccaaacgc cagacggtca gtggcgcaca caatagcgtc cggcttcatt tcctccagga
     7021 tctctctcgt atttttctgt cctccgagaa aactgtaatc actctttttt atcagcggat
     7081 catgaattcc aaatgccgca agtccgtcca gaactccctg acgccttcgg atacctacgg
     7141 cctcatcggt ctcataaaca ccaatatatc caacgttctt atgacctgtt tttccgatat
     7201 attctcccac acattttcca gcattgtaat catcatcgat gatagaaatc ccgtcctcat
     7261 aatcctgagc caggacaaca atgggtatct gtgtctgctc aaaaatatca cggtgatcct
     7321 ctgtgactgc gatagaactg atcaaaatcc catccacatt cagatgaatc agcttttgta
     7381 tatttaaaag ctccagatca atatcatgat ctgagttttt gatcagtgtt gtgtaattcc
     7441 tgtcccttaa ataccggtca atgcttgtga tgacacggct ggtaaccttg gaattcagcg
     7501 taggcactac gaccccgatc acattgcttt cctttgcctt taaccttgca aatgtattcg
     7561 gtgcataatt atattcttcg attactttct gaatcttttc cctcgtagcc tttttgacat
     7621 atcccccatt aaaataacgg gaaaccgtgc tttttgtcac tcccgccatc tctgccacat
     7681 cagccattgt tatttttttt ggcattgtgt atcctgcctt tctctcatac ttgattttat
     7741 tgtaagatga tttcccggat tgtgcaaccg gaatccggtt tctttccatt ttctttgaca
     7801 tttcataatt tatgtaatat attacatata ggaggtattt cgaccatgaa attagaagat
     7861 tacgacagca gacacctgct gtttgctgca ttatttatga ccagtaaccg gctccagtca
     7921 atcggagacc gcttctatga agagatcacg tcaaaacaat ggtttcttct ggctgctctc
     7981 aatacctttg agtatgcccc aactttcaat gaactttcct cccttatggg cagttcccac
     8041 caaaatgtaa agcagatcgc tttaaagctt cagcagaaag gttatgcgga aatagaaact
     8101 gacccggatg accgccgcaa aagccgggtg cgccttggca gaaaagccga atcactgcag
     8161 tctgcgtatg ccttaaagga agaagctttt atggatcttt tatacaagga tatctcagag
     8221 acacagctga agaccgcact ggaaaccctg aatactatga tgaaaaacat ggaggaatgt
     8281 aatatatga
//

