LOCUS       NZ_AP022379            11900 bp    DNA     linear   CON 13-APR-2021
DEFINITION  Bifidobacterium longum subsp. longum strain JCM 7052 chromosome,
            complete genome.
ACCESSION   NZ_AP022379 REGION: 35557..47456
VERSION     NZ_AP022379.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00200813
            Sequence Read Archive: DRR266978
            Assembly: GCF_015100215.1
KEYWORDS    RefSeq.
SOURCE      Bifidobacterium longum subsp. longum
  ORGANISM  Bifidobacterium longum subsp. longum
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1
  AUTHORS   Yamamoto,I., Ueno,Y., Geshi,M., Inagaki,Y., Odamaki,T. and
            Fujita,K.
  TITLE     Complete genome sequence of Bifidobacterium longum subsp. longum
            JCM7052
  JOURNAL   Microbiol Resour Announc 10, e01193-01120 (2021)
  REMARK    DOI:10.1128/MRA.01193-20
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   Ueno,Y., Geshi,M., Inagaki,Y., Odamaki,T., Fujita,K. and
            Yamamoto,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2020) Contact:Kiyotaka Fujita Kagoshima
            university, Faculty of Agriculture; Korimoto 1-21-24, Kagoshima,
            Kagoshima 890-0065, Japan
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            AP022379.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Velvet v. 1.2.10; Gapcloser v. 1.10
            Genome Coverage       :: 2,500X
            Sequencing Technology :: Illumina HiSeq2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 04/13/2021 19:53:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 73
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 23 of 57
            Pseudo Genes (incomplete)         :: 34 of 57
            Pseudo Genes (internal stop)      :: 5 of 57
            Pseudo Genes (multiple problems)  :: 5 of 57
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Bifidobacterium longum subsp. longum"
                     /mol_type="genomic DNA"
                     /strain="JCM 7052"
                     /isolation_source="human feces"
                     /host="Homo sapiens"
                     /sub_species="longum"
                     /db_xref="taxon:1679"
                     /country="Japan"
                     /collection_date="1987"
     gene            complement(1..1014)
                     /locus_tag="BLGA_RS00155"
                     /old_locus_tag="BLGA_00290"
     CDS             complement(1..1014)
                     /locus_tag="BLGA_RS00155"
                     /old_locus_tag="BLGA_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="WP_101014018.1"
                     /translation="MEASSTEKKRRATRADVARLAGVSTAVVSYVFNNGPRPVAADTA
                     QQVREAAEKLHYRPNSVARALSTGSSKTLGIVVPDLSNPFFSGVYSALETAAARNGYS
                     TLFMASHQNPEKEMDRISQLIAREVDAIIIASAQPTSALAGVPRKECPFIFMDQTQPV
                     PGAKCVSTDFQAAVSLAVRHLLGHGYTNIAMLSGKADDGLSDKRIQGWYQAHKETEIP
                     VGPVVQAHFTRQGGYDATLALLDSDHRPDAIFADSDLEAIGALRALHERHVRIPEDIA
                     IVSFDGTVDSQFSWPALTVVQQDATAIAERMFQAALDPQNTPDLQLIDTTLVPRQSCG
                     CLK"
     gene            1353..2612
                     /locus_tag="BLGA_RS00160"
                     /old_locus_tag="BLGA_00300"
     CDS             1353..2612
                     /locus_tag="BLGA_RS00160"
                     /old_locus_tag="BLGA_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="WP_077381867.1"
                     /translation="MKSIIKGIAAVAACAMTVTGLAACGSSSASGEKKLDFITGMATG
                     SVHLKTLQSITDAFEKENPGVKINLIPSSQDFTQDIKVRLAARNAPDLWNTHGWSRDR
                     YADFLEPLQNRSWAGKMKDLTKEAFMEDDGTFYALPLDIQVTGVLYNKDVLDKVGVDP
                     NSLNTWDEFNDACAKVKEAGLTCVVAGGKDTAIAGDLADLTASAWYDDAELKNLQSGK
                     FDSSVYEKESGLIEGWKNNGYFNKDYTSATQDDIEKLMANGQAAFYFRSNLHSAQIET
                     FNPDVNLGFMATPTESGDRYVTIGEDWAVGASKTGKNKDIALKYIDFLAEPENMTKLT
                     KMTNNDSALDGIDVNLGKINDTYDYWVNEKQTRTVPFFDRIYLPDGMWDTLCKSTDGV
                     ITGQLDAKGAADQMSNSFTTMWAKKQS"
     gene            2791..3717
                     /locus_tag="BLGA_RS00165"
                     /old_locus_tag="BLGA_00310"
     CDS             2791..3717
                     /locus_tag="BLGA_RS00165"
                     /old_locus_tag="BLGA_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_077381933.1"
                     /translation="MSNKTLSSRGRQEAQSKRAGRFRGSSINLFYLPAVILLAVFIVY
                     PLFSGIGLSLTNWDGYSPEKTFIGLTNFAQMFKDANFRLILINTLIYGVGSTAIQQVL
                     GLGLALLLNSKIRGRNVFRAIIYLPALVAPVIMGTMYYFIFQYQQGALNTIMTGLGFE
                     KVAWFNNPAVSVAIITIVNSIQFVGVSMIIYLAGLQTLDQSVMEAASLDGASGWKKFW
                     NITLPMLQPAFTTSVVLNLIGGLKLFDIIKVLTNGGPGYATNSISTYISSMYFDAQNA
                     GYASALGVFLFILIAIITYLLNTGLAKLNWEA"
     gene            3719..4582
                     /locus_tag="BLGA_RS00170"
                     /old_locus_tag="BLGA_00320"
     CDS             3719..4582
                     /locus_tag="BLGA_RS00170"
                     /old_locus_tag="BLGA_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_077381865.1"
                     /translation="MSTTKRINQKKVDQIGYQRGRWAIYLFLAIVVVCQLLPFYLAIN
                     ASMKPQTDMSSTLMPRLHDIAWDNWTAAITDGQILHSVVNSVIVTVCTTLIVCVVGAA
                     AAYPLARRATRFNKVISALILATMMVPPLSILVPLYTMLVNLGGANTYWGIVLVLAAI
                     NMPLSVFLYTAFIRSIPVSVDEAGMIDGANRFTIFVRLILPMLKPVTATVIIMTGTTV
                     WNDYALSVYLLTDADKQTIAPRVASFFAANSSNLGTAAAAALIAAAPMVIAYLFLQKY
                     FIAGMIAGAEK"
     gene            4613..6763
                     /locus_tag="BLGA_RS00175"
                     /old_locus_tag="BLGA_00330"
     CDS             4613..6763
                     /locus_tag="BLGA_RS00175"
                     /old_locus_tag="BLGA_00330"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="WP_077381863.1"
                     /translation="MLFPSVSVPGELTRIALSDVHAGDDLPNVNALLSTDDPTAYADA
                     PESTPRAVPLLTEHALNTFTRPGLRGFRITVDDAEDEASAADFAALMQLGGGWTPRFA
                     IAQEPEVSDRRLAYRAVDESAGLELGTELESLAGGSLRIRHTLTNRADSPYVVQGLDV
                     SVPLADNQTELLDFTGRHERERQPQRHTIADGIWSREFRWGKTGFEGPLFVAGTPGFD
                     FAHGSVLMVQPAWSGNNELYVQRDMAQIATVSAGELLEPGEIVLRQGESYATPWIMVT
                     ASNSGLDGAAASLHQWERSLPAHPRRQPVVLNVWEGVMFDHNLDTLLEIARRAAAIGV
                     ERYVLDDGWFHLRRDDHAGLGDWWVDPDVWPEGLHPLVDFVHEQGMQFGLWFEPEMVN
                     PDSDLYREHPDWVLQAAPRTPILQRHQLVLDLANPEAFDHVYRAIHDVLSQYRIDYIK
                     WDHNRYLLEAGSNLHGGAASIHNQTVAYYRLLDKLRADFPDIEWESCASGGGRIDTGV
                     IEHVQRCWTSDMTDALSRQCIQRWTVQNIAPEYLGAHISQPTSQQTGRTYSVAFRAAT
                     AVFHSFGIEWDITKASDADLQELASWIVWYKANRDFLHSGRFVRLDVADPAVLAHGVV
                     AADGSRALIAHVQYEESSSNRGVWLRVPGLDADARYALRWVGPEPARASLEPIDPLGP
                     VGSRSVSGAWLASPGVRVPRCRPETVRLVEIVKV"
     gene            7101..10388
                     /locus_tag="BLGA_RS00180"
                     /old_locus_tag="BLGA_00340"
     CDS             7101..10388
                     /locus_tag="BLGA_RS00180"
                     /old_locus_tag="BLGA_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="WP_157821655.1"
                     /translation="MQTLNVDYGTTTGDFYGGSSGMLYGLGDEGSPTDAILDGARVQV
                     TSQKPADGLQHPSADVLAVENQFFNNGGEELVVNLQDWYPDWSYNGGKRPGDTRSYKL
                     DVDPNDAQYGTYTDNAPNDRWDFDEVLEVVMNKILANTSHPDNIVFMPFNEPDGGNWY
                     ASGDNASADIYKQFLTDWNDAYATIQKVWNQYKSGEKTNVNGVKPTADHALIAGPGDS
                     VWRANRTKALLESSKAANTLPDVIVWHELGNGSLKNYRSHYNQYRSFEQELGITPRAI
                     NISEFGELRDMSVPGQLIQWMSMFESTKVQAQTAYWNYAGNLNDNMARANSANGGWWL
                     YKWYGDLRGTQTVKVTSEHPDSVDNLQGVAAIDTKNRKATVLYGGANDATKIGANIPV
                     TVHMTGLDQSVFGESVDVQVRENAYTGPDGVAATPRVVNVLSDQKLVNGTLDVTTTSI
                     DRYAGYQLVVTPHQDVATSLDNAENGRSLQAIEAEDTTLSDGAKAYTKSDNTGSWGNF
                     MFSGNGDVGNFKNGAKMTWNVDVPADGAYRLQLITAPAGFPGVNHVYVDGKSVGDLNV
                     PAELALKEAAKWKYRGSAEIVLDGLTKGKHAITVEATDLDNESDKLLLYQVSRGDGTP
                     VDQVTYPASDMRLDSGAVLAYDGNGTNGFANLNGGRADVFAHAWEAGYQNVTIAYNAA
                     KGAHFVLSVNGQSAATVTAQSDGAQTSIVRVAFSEGINQLTLSGAAGVRVASVTTARA
                     SESDSKAIRFEAEDAQLSGGAKVQKDDASDASGKSFVAGLGNQFVTNESGKAGYGDQT
                     RVEADANHVPTVAQNNKGTMTVTGVPAGAYNMVVRFSNDAFIGSHSYNPQVVDLGLQV
                     REGDQEIARGSFRYTYTEKHFMNRSVTVTTHGGALTLGNWDEVGSRKAAVSWGVAPNV
                     DSITFYPITDGNVIDSVSGVTVSGEGVSADGKASLKVGETLSLNASVTPDSVADKTVQ
                     WTSSDEQVATVDEHGVVKGVKAGTVTITATSVADSSRSGSVEVTVAEDSEQKPSGGDG
                     DNNGEQTGKPDGNTGGQTSDSDAGADSGNNQKHMPLTGAAVAAVAGVAVLLAGAGLLL
                     KRLRSRS"
     gene            10590..11900
                     /locus_tag="BLGA_RS00185"
                     /old_locus_tag="BLGA_00350"
     CDS             10590..11900
                     /locus_tag="BLGA_RS00185"
                     /old_locus_tag="BLGA_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007055811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_198545768.1"
                     /translation="MTMDRTAMKQLEAWKTSRNRKPLIIRGARQTGKTWLSEEFGRTR
                     YEAVARIDLMNNERARSFFDGDLDVSRILRNISLETGVPITADTLVLLDEIQECPRAL
                     TALKYFCEDARAYHVIATGSYMGIARHEDTSYPVGKVDTLTLRPMDFTEYLRAIGQGM
                     MADAMSDGASIADLDSGFRDRLKDWLKEYMVVGGMPAVVADFAEHHDFNEARKLQREI
                     LDDYDGDFSKHAPLRILERIRMVWASLPSQLAKENRKFIYGALRPGARAREFEESIQW
                     LRDYGAITKVPAVTAIRTPLAAYEDSAAFKLFAVDCGLLGALARLSPSTILEGEKLFT
                     EFKGAFTEQYVCQQLVAQRLSPYYWSSPTGRAEVDFVVDHPDTVLPIEVKAQENLQAK
                     SLRVAVGHFDLHKAVRTSLAGYRDNDWLLNVPLWAINRLAALPL"
ORIGIN      
        1 ctatttcagg catccgcagg actgacgggg aaccagcgtg gtgtcgatga gctgtaggtc
       61 gggggtgttt tgggggtcaa gcgcggcttg gaacatgcgc tcggctatgg cggtggcatc
      121 ctgctgcacg acggtaaggg caggccagct gaactgtgag tcgacggtgc cgtcgaagga
      181 gacgatggcg atgtcctcgg ggatgcgtac gtgccgttca tgcagcgctc gcaatgcgcc
      241 gatcgcctca agatcggaat ccgcgaaaat cgcatcgggt cggtgatccg aatccaagag
      301 cgcaagggtg gcgtcataac cgccttgacg agtgaagtgg gcctgtacga cgggcccgac
      361 gggaatctca gtctccttgt gcgcttggta ccaaccttgt atgcgtttgt cactcaagcc
      421 atcatcggct tttccggaca gcatggcgat gttggtatac ccatgcccca gcaggtgccg
      481 taccgctagg cttaccgccg cttgaaaatc cgtcgatacg catttcgcgc cgggaaccgg
      541 ctgggtctga tccatgaaga tgaacggaca ctcctttcgt ggcactcccg ccagtgccga
      601 agtgggctga gccgaggcga tgatgatggc atccacctct cgcgcgatca gctgggagat
      661 gcggtccatc tccttttcgg gattctgatg agatgccatg aacagggtcg agtagccatt
      721 gcgcgccgcg gcggtttcca gtgcgctgta gacgccggag aagaacgggt tggacaaatc
      781 gggcacgacg atgccgaggg tcttcgatga cccggtgctc agggcgcgtg caaccgaatt
      841 ggggcgataa tgcagctttt cagctgcctc gcggacctgt tgggcggtat ccgccgcaac
      901 cggacgcggt ccgttgttga acacatagct gactaccgct gtggagacgc ctgcgaggcg
      961 ggcgacatcg gccctggtcg ccctacgttt cttctcggtt gatgacgctt ccataactcc
     1021 tcgttcctgt gcaatgcgtg tagtcgtgtg gaacatctaa cgacgtaact catagatagt
     1081 ctacggttga ttctacgcgc gtcatttttg tagacacgcg gtggttgcgg tattcgttac
     1141 tggtagaagg tggaagtatc cttgtgaaat attgaaaaat aaaggttttt gcaaattgcg
     1201 gagcgatatg acgcgccgcg caactccgta gacttgcgat tgagttctac ccgcgtagaa
     1261 tcagtaatca ctgatcggcc ggcacgccgg acgaaaggcg cagcagcctt tcggatcagc
     1321 atccgaaaag gaaggaacag cgaggtttga acatgaagag catcatcaaa ggtatcgcgg
     1381 cggtcgccgc atgtgccatg acagtcactg gactcgccgc ctgcggaagc agctccgcca
     1441 gcggggaaaa gaagctggac ttcatcaccg gcatggccac cggatccgtg catctcaaga
     1501 cgctgcagtc catcactgat gcatttgaga aggagaatcc aggcgtcaag atcaatctga
     1561 ttccgtcttc ccaggacttc acccaggaca tcaaggtgcg tctggcggca aggaacgcgc
     1621 cggatctgtg gaacacccac ggttggtcgc gcgatcgcta cgccgacttc cttgagccgc
     1681 tgcagaaccg cagctgggcc ggcaagatga aggacctcac caaggaggcc ttcatggagg
     1741 acgacggtac gttctatgcg ctgccgcttg atatccaggt caccggcgtc ctctacaaca
     1801 aggacgttct cgacaaggtc ggtgtagatc cgaactccct gaatacatgg gacgaattca
     1861 acgacgcatg cgccaaggtt aaggaagccg gtctgacctg cgtggtcgcg ggtggcaagg
     1921 acaccgccat cgccggcgat ctcgccgacc ttaccgcctc tgcttggtac gacgatgccg
     1981 aactcaagaa cctgcagtcc ggtaagttcg attcttccgt ctacgaaaag gaaagcggcc
     2041 tgattgaagg ttggaagaat aacggctact tcaacaagga ctacacctcc gccacccagg
     2101 acgacatcga gaagctcatg gccaacggtc aggcggcctt ctacttccgc tccaacctgc
     2161 actccgccca gatcgagacc ttcaacccgg acgtgaacct cggcttcatg gccacgccga
     2221 cggaaagcgg cgatcgctac gtcaccattg gcgaggactg ggcagtcggc gcatccaaga
     2281 ccggcaagaa caaggacatc gcgttgaagt acatcgactt cctcgctgag ccggagaaca
     2341 tgaccaagct caccaagatg accaacaacg actccgccct ggacggcatc gatgtgaacc
     2401 tcggcaagat caacgacacc tacgactact gggtcaacga gaagcagacg cgtaccgtgc
     2461 cgttcttcga ccgcatctac ctgcctgacg gcatgtggga taccctgtgc aagtccaccg
     2521 atggtgtgat caccggtcag ctcgacgcca agggcgcggc cgatcagatg agcaacagct
     2581 tcaccaccat gtgggccaag aagcaaagct gaccaatgcc ggttcgaacc gatcgatgac
     2641 gtccaggtct tccggaaccg tcgctcccct cccctcccct gaaggagcgg aacggcggtt
     2701 ccgggcggat gtcatcgcat gaaccgcaag ggattgccgt caatccgcca aatcatcaca
     2761 tcgaatcatc gaatcggaag catatccatc atgagcaata agacgttatc gtcgcgcggc
     2821 cgtcaagagg cgcaaagcaa acgcgcaggc cgattccgcg gctcctccat caacctgttc
     2881 tatctgccgg cagtgattct gctcgccgtc tttatcgtct atccactgtt ttccggcatc
     2941 ggactgtccc tgacgaactg ggatggttac agccccgaga agacgttcat cggactgaca
     3001 aacttcgcgc agatgttcaa agacgcgaac ttccggctca ttctcattaa cacactgatc
     3061 tatggcgtcg gatcgaccgc catccagcag gtcctcggcc tcggtctggc gctgctgctg
     3121 aactcgaaga tccgcgggcg caacgtgttc cgcgccatca tctaccttcc cgcacttgtg
     3181 gccccggtca tcatgggtac gatgtactac ttcatcttcc agtaccagca gggcgcgctg
     3241 aacacgatca tgaccggcct cggcttcgag aaggtggcgt ggttcaacaa cccggccgtc
     3301 tccgtggcga tcatcaccat cgtcaattcc atccagttcg taggcgtgtc gatgatcatc
     3361 tatctggcag gtctgcagac cctcgaccag tcggtgatgg aggccgccag cctcgatggc
     3421 gccagcgggt ggaagaaatt ctggaacatc acgctgccca tgctgcagcc cgccttcacc
     3481 acgtccgtcg tgctgaacct catcggcggc ctcaagctgt tcgacatcat caaggtgctc
     3541 accaacggcg gcccgggata tgcgacgaac tcgatctcca cctacatcag ctcgatgtac
     3601 ttcgatgcgc agaacgccgg atacgcgtct gcactgggtg tgttcctgtt catccttatc
     3661 gcgatcatca cctacctgct caatacgggt ctggcgaagc tcaactggga ggcctgagat
     3721 gtccaccacc aagagaatca atcagaagaa agtcgatcag atcggctacc agcgtggacg
     3781 ttgggccatc tacctgttcc tcgccatcgt ggtggtatgc cagctgctgc cgttctactt
     3841 ggcgatcaac gcctcaatga agcctcagac ggacatgtcg tccacgctga tgccgcgcct
     3901 gcatgacatc gcatgggaca attggaccgc cgccatcacc gatggtcaga tccttcattc
     3961 agtggtcaac agcgtcatcg ttactgtatg caccacactg attgtctgcg tggtcggtgc
     4021 cgctgcggcc tatccgctcg cccgtcgagc cacgcgcttc aataaggtca tctccgcgct
     4081 gattctggcc acgatgatgg tgccgccgct gagtattctt gtgccgctgt acacgatgct
     4141 tgtcaatctg ggtggcgcta acacctactg gggcattgtt ctggtgctgg ccgccatcaa
     4201 catgccgttg tcggtattcc tgtataccgc gttcatccgt tcgattcccg tgtccgtgga
     4261 tgaggctggc atgatcgatg gtgcgaaccg cttcacaatc ttcgtgaggc tgattctgcc
     4321 gatgctcaag cccgtcactg cgactgtcat catcatgacc ggcacgaccg tctggaacga
     4381 ctatgcgctg tcggtctacc tgctcaccga cgccgacaag cagacaatcg caccgcgcgt
     4441 cgcctccttc ttcgctgcga acagcagcaa cctcggtacc gctgcggctg ccgcactgat
     4501 cgccgccgct ccgatggtca tcgcctacct gttcctgcag aagtacttca tcgccggcat
     4561 gatcgccggt gcggagaagt gacgttcgga atcaaacctg tagggagaag tcatgttgtt
     4621 tccttccgta tcggttcccg gggaactgac cagaatcgct ctgtccgacg tccatgccgg
     4681 tgacgatctg cccaacgtca acgcactgtt gagcacggat gatccgaccg cgtacgccga
     4741 cgcccctgaa tcgactccgc gtgcggtacc gctgctcacc gagcatgcgc tgaatacctt
     4801 cacccgtccg ggattgcgcg gtttccgcat tacagtcgat gatgccgagg atgaagccag
     4861 tgctgcggac ttcgcggctc tgatgcaact cggtggaggc tggaccccac gattcgccat
     4921 cgcgcaagaa cccgaagtca gcgatcgtcg gttggcatat cgcgctgtcg atgagtccgc
     4981 aggactcgaa ctcggcactg aactcgaatc cttggctggt ggttctctgc gcatacggca
     5041 cacgctgacc aatcgtgcgg attctccgta cgtggtgcag ggccttgacg tcagcgtgcc
     5101 attggccgat aaccagaccg agctgctgga cttcaccggc cgccacgaac gcgaacgcca
     5161 gccacagcgc cacaccattg ccgacggcat atggtcgcgg gaattccgat ggggcaaaac
     5221 cggcttcgaa ggcccgttgt tcgtggccgg cacgccagga ttcgatttcg ctcacggttc
     5281 ggtgctcatg gtacagcctg catggagcgg caacaacgaa ctgtatgtgc agcgcgacat
     5341 ggcgcaaatc gccactgtca gcgccggcga actgctggag ccgggcgaga tcgtgctgcg
     5401 ccaaggggag agctatgcca cgccgtggat catggtcacg gcatccaact ccggtcttga
     5461 tggtgccgcc gccagcctgc atcagtggga gcgctcgcta cctgcgcatc cgcgccgcca
     5521 accggtggtg ctcaacgtgt gggaaggcgt gatgttcgac cacaatctgg atacgctact
     5581 ggagattgcc cgtcgtgccg ccgctatcgg ggtggagcga tacgtgctcg acgacggttg
     5641 gttccatctg cgccgtgacg accatgccgg cctcggcgat tggtgggtgg accccgatgt
     5701 gtggcctgaa ggattgcatc cgctcgttga tttcgtgcat gaacaaggaa tgcaattcgg
     5761 cctgtggttc gagccggaga tggtcaatcc tgattccgac ctataccgcg aacaccctga
     5821 ttgggtgttg caggcggcgc ctcggacccc gattctccag cgtcaccagc tcgtgctcga
     5881 ccttgccaat cctgaagcat tcgatcatgt gtatcgcgcc attcacgatg tgctgtccca
     5941 ataccgcatc gattacatca agtgggacca caaccggtac ctgcttgagg cgggctcgaa
     6001 ccttcatggg ggagcggcgt cgatccataa ccagaccgtg gcctattacc gtctgctgga
     6061 caagctccgc gctgatttcc ctgatatcga atgggaatcc tgcgcatccg gcggcggccg
     6121 catcgatact ggcgttatcg aacacgtaca gcgctgctgg acttcggaca tgactgacgc
     6181 gctcagccgc caatgcatcc agcggtggac cgtacagaac atcgcgcccg aatacttggg
     6241 cgcgcatatc tctcagccta cttcgcagca gaccggccgt acgtattcgg ttgcgttccg
     6301 tgcagcaacg gccgtgttcc acagcttcgg tatcgaatgg gacatcacca aagccagcga
     6361 cgcggatcta caggagcttg cctcttggat cgtttggtac aaggccaatc gtgacttcct
     6421 gcattccggt cggttcgtgc ggctcgatgt ggcggacccg gccgtgctcg cacatggcgt
     6481 ggtggctgcc gatggctcgc gtgcgcttat cgcgcacgtg cagtacgagg aatcgtcctc
     6541 gaaccgcggc gtatggctgc gcgtgcctgg actcgacgcc gatgcgcgtt atgcgctgcg
     6601 ctgggtgggc cccgagccgg cgcgggcatc gttggagccg atcgacccgc tcgggccggt
     6661 ggggtcgcgc tcggtatccg gagcgtggct ggcgtccccg ggcgtgcgtg tcccgcgatg
     6721 ccggcccgaa acggtgagac tggttgaaat cgtcaaagtc tgacgtttcc acaagcgctc
     6781 gcatcacact gtaggagttt cacttgcgcg atgggcgcga tgcggactcc cgcaaatccc
     6841 agacttcttt ccttgcggtt ttctccttct cgtatggcag gaagcccggg ttttcatatg
     6901 gcaagaccgc gtgtaaagcg catgctgtat tggtctgcca ccgtttcaat gaagaaaggt
     6961 acaggaaggt acacactatg ggtatatcca gaaataggct ggtgccaggg ctggtaggct
     7021 tggctgccag cgcggcgatt gttctgccgc ttggcatagg aatgccagtc agctcggcga
     7081 cggcggagga gaatgcgcca gtgcaaacgt tgaatgttga ttacggcacg acgaccggcg
     7141 atttctatgg cggctcgtcc ggcatgctgt acggcttggg cgatgagggt tcgcccacgg
     7201 acgccatcct cgatggcgct cgcgtgcaag taacttcgca gaagcccgcc gatggtctgc
     7261 agcaccccag cgccgacgtg ctggcggtgg agaaccagtt cttcaacaac ggcggcgagg
     7321 agctggtggt caaccttcag gattggtacc cggactggtc ctacaacggc ggcaaacgcc
     7381 ccggcgatac gcgctcctac aagttggacg tggatcccaa tgatgcgcag tatggcacct
     7441 acacggacaa cgcacccaac gatcgctggg acttcgacga agtgctcgaa gtggtgatga
     7501 acaagattct ggccaacacc tcccaccccg acaatattgt attcatgccg ttcaacgagc
     7561 cggatggcgg caactggtac gcttccggcg acaatgccag cgccgatatc tacaagcaat
     7621 tcctcaccga ctggaatgat gcctacgcca cgattcagaa ggtgtggaat cagtacaagt
     7681 ccggcgagaa aaccaatgtg aacggcgtca agccaaccgc cgaccatgcg ctgatcgccg
     7741 ggcccggcga cagtgtgtgg cgtgcgaacc gcaccaaggc tttgctggaa agctccaagg
     7801 ccgccaacac cttgcccgat gtgatcgtct ggcacgaact cggcaacggc tcgctgaaga
     7861 actatcgcag ccactacaac cagtaccgca gcttcgaaca ggaacttggc atcacgccgc
     7921 gtgccatcaa catctccgaa ttcggcgaac tgcgtgacat gtccgtgccc ggccagctga
     7981 tccaatggat gagcatgttc gagagcacca aggtgcaggc gcagaccgcg tactggaact
     8041 acgccggcaa cctcaacgac aatatggccc gcgccaactc ggccaacggc ggttggtggc
     8101 tgtacaagtg gtatggcgat ctgcgcggca cgcagaccgt caaggtgacc agtgagcatc
     8161 cggatagcgt agataacctg cagggtgtgg ctgctatcga caccaagaac cgcaaggcca
     8221 ccgtgctgta cggcggcgcc aacgatgcca ccaagatcgg cgcgaatatt ccggtcaccg
     8281 tgcacatgac cggactggac cagtcggtgt ttggcgagag cgtggatgtc caggtgcgtg
     8341 agaacgccta caccggtccg gacggcgtcg ccgcaacccc gcgagtggta aacgtgctga
     8401 gcgatcagaa gctcgtcaat ggcacgcttg acgtgaccac caccagcatc gatcgctacg
     8461 caggctatca gcttgtggtg acgcctcatc aggatgttgc cacttcgctg gacaacgccg
     8521 aaaacggtcg ctcgctgcag gccatcgaag ccgaggacac cacgctgtct gacggtgcca
     8581 aggcatatac gaagagcgac aataccggtt cctggggcaa cttcatgttc tcgggcaatg
     8641 gggatgtcgg caacttcaag aacggtgcca agatgacgtg gaatgtggac gtgccggctg
     8701 acggcgccta ccgtctgcag ctcattaccg ctccggccgg cttcccgggc gtcaatcatg
     8761 tgtacgtcga cggcaaatcg gttggtgatc tgaatgtgcc tgccgagctg gcgctgaagg
     8821 aggccgccaa gtggaagtat cgcggcagtg cggaaatcgt gctggacggt ctgaccaagg
     8881 gcaagcacgc cattacggtg gaagccactg atctggacaa tgaatcggac aagctgctgc
     8941 tctaccaggt ctcccgtggt gacggtacgc cggtcgacca ggtgacctac ccggcctcag
     9001 acatgcgctt ggattccggc gcggtactgg cttatgacgg caacggtacg aacggattcg
     9061 ccaacctcaa tggcggccgt gcggatgtct tcgctcatgc gtgggaggcc ggctaccaga
     9121 atgtgacgat tgcctacaac gccgccaagg gcgcccactt cgtcttgtct gtgaatggac
     9181 agagtgcggc tactgtcacc gcccaatcag acggtgcgca gacctcgatt gtgcgcgtgg
     9241 cgttctctga aggtatcaac cagctcaccc tgtccggtgc ggcaggggtg cgtgtggcga
     9301 gtgtcaccac cgcgcgtgcg tcggagtcgg attccaaggc gattcgcttc gaggccgagg
     9361 atgcgcagtt gagcggcggg gccaaggtcc agaaggatga tgcctcagac gccagcggca
     9421 agtcgttcgt cgccggattg ggcaaccagt tcgtgaccaa cgagagcggc aaggccggct
     9481 acggcgacca gactcgtgtt gaagccgatg ccaaccacgt gcccaccgtc gcgcagaaca
     9541 acaagggcac gatgaccgtg accggtgtac cggccggcgc atacaacatg gtggtgcgct
     9601 tctccaacga tgcgttcatc ggcagccact cctacaaccc gcaggtcgtg gatctagggc
     9661 tccaggtgcg cgagggtgat caggagattg ctcgtggctc gttccgatac acctacaccg
     9721 agaagcactt catgaaccgt tcggtgaccg tgaccactca tggtggcgcg ctgaccctcg
     9781 gcaactggga cgaagtcggc tcgcgcaagg cggcggtctc gtggggcgtc gcgccgaacg
     9841 tggattccat cacgttctac ccgattacgg acggtaatgt gatcgactcc gtgagcggtg
     9901 tgacggtgag cggcgaaggc gtttccgccg atggcaaggc ctcgctcaag gtgggggaga
     9961 cgctgtcact gaacgcctcg gtcactcccg attccgttgc agacaagacc gtgcagtgga
    10021 cttcgtccga cgagcaggtt gcgacggttg acgagcatgg cgtggtcaag ggcgtgaagg
    10081 ccggcacggt gaccatcacc gccacttccg tggccgattc ctcgcgcagt ggctccgttg
    10141 aggtgacggt tgccgaggat tcggaacaga agccctcggg cggcgacggt gacaacaacg
    10201 gcgagcagac cggcaagcca gatggaaaca ccggcggtca gacctctgat tccgatgcgg
    10261 gcgccgactc tggcaataac cagaagcata tgccgctcac cggtgctgcc gtggcagctg
    10321 tggcaggtgt tgccgtgctg ctggctggcg cggggctgct gctcaagagg ctgcgcagcc
    10381 gtagctgatt gcagattttg gccggggctg ttttctccca gtcagcctgc gcccgacagc
    10441 tcccctcaag gggagctgtc gttttttgct ctgtattatg cggtagcgtt ggtccttccg
    10501 ctataactag cgcggaacaa tggtttgact cacacatttc ttgtacataa aatgtgtttt
    10561 actcgcacat tttttgtagg agattgaggt tgactatgga tcgtacggct atgaagcagc
    10621 ttgaggcctg gaagacatct cgaaatagaa agccgctcat tatccgaggt gcccgtcaga
    10681 cgggcaaaac gtggctgtcg gaggagttcg ggcgtacacg atatgaggca gtggcgcgta
    10741 tcgacctcat gaacaatgag cgtgcgagga gtttctttga cggcgattta gatgtgagca
    10801 ggatattgcg taacatcagc ctcgaaacag gtgttcctat cacggcagat accttggtgc
    10861 tccttgatga gattcaagaa tgtccgcgcg cattgactgc gctgaagtat ttctgcgaag
    10921 atgctcgtgc atatcacgtt attgcgactg gctcatacat gggtattgcc agacatgaag
    10981 acacttcgta cccggttggc aaagtcgata cgttgacatt gcgtccaatg gatttcacgg
    11041 agtaccttcg cgccatcggc caaggaatga tggctgatgc catgagcgac ggcgcaagta
    11101 ttgcagattt ggattccgga ttccgggatc ggcttaagga ctggctgaag gaatatatgg
    11161 ttgttggcgg aatgccggca gtagttgcgg atttcgcgga acatcacgat ttcaacgaag
    11221 cccgtaaatt gcagcgtgag attcttgatg attatgacgg ggacttctcg aaacatgcgc
    11281 cgctacgtat ccttgagaga attcgtatgg tttgggcttc tctaccatcg cagctggcaa
    11341 aggagaatcg aaagttcata tacggtgcct tgcggcccgg cgcacgcgct agggaattcg
    11401 aagaaagcat tcagtggctt cgcgattatg gagccattac aaaagttcca gcggtaacgg
    11461 ctattcgcac gccattagcg gcgtatgagg actccgctgc gttcaagttg ttcgctgtgg
    11521 actgtggtct attgggggcg ctggctcgcc ttagtccatc gacgatcctt gagggagaga
    11581 aactatttac tgagttcaag ggagccttca ctgagcaata cgtatgtcag cagctcgtgg
    11641 cgcaaaggct atccccgtat tattggtcgt cgccaacggg tcgtgcggaa gttgattttg
    11701 tcgttgacca tccagatacg gttctcccca ttgaagtaaa ggcgcaagag aaccttcaag
    11761 ccaagagcct gcgtgtggct gttggccatt tcgacctgca taaagcagtg cgcaccagtc
    11821 ttgctgggta tcgtgacaat gactggctgc tgaatgtgcc attatgggcg attaatcggc
    11881 ttgctgcatt gccgctatag
//

