LOCUS       NC_008255               8764 bp    DNA     linear   CON 05-OCT-2022
DEFINITION  Cytophaga hutchinsonii ATCC 33406, complete sequence.
ACCESSION   NC_008255 REGION: 1051545..1060308
VERSION     NC_008255.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02598536
            Assembly: GCF_000014145.1
KEYWORDS    RefSeq.
SOURCE      Cytophaga hutchinsonii ATCC 33406
  ORGANISM  Cytophaga hutchinsonii ATCC 33406
            Bacteria; Bacteroidota; Cytophagia; Cytophagales; Cytophagaceae;
            Cytophaga.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Xie,G., Bruce,D.C., Challacombe,J.F., Chertkov,O., Detter,J.C.,
            Gilna,P., Han,C.S., Lucas,S., Misra,M., Myers,G.L., Richardson,P.,
            Tapia,R., Thayer,N., Thompson,L.S., Brettin,T.S., Henrissat,B.,
            Wilson,D.B. and McBride,M.J.
  TITLE     Genome sequence of the cellulolytic gliding bacterium Cytophaga
            hutchinsonii
  JOURNAL   Appl Environ Microbiol 73 (11), 3536-3546 (2007)
   PUBMED   17400776
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
            Brettin,T., Bruce,D., Challacombe,J.F., Chertkov,O., Han,S.,
            Tapia,R., McBride,M.J., Misra,M., Myers,G.L., Thayer,N.N.,
            Thompson,L.S., Xie,G., Gilna,P., Schmutz,J., Larimer,F., Land,M.,
            Hauser,L., Kyrpides,N., Lykidis,A., Ivanova,N., Mavrommatis,K.,
            Rubin,E. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Cytophaga hutchinsonii ATCC 33406
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8764)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
            Brettin,T., Bruce,D., Challacombe,J.F., Chertkov,O., Han,S.,
            Tapia,R., McBride,M.J., Misra,M., Myers,G.L., Thayer,N.N.,
            Thompson,L.S., Xie,G., Gilna,P., Schmutz,J., Larimer,F., Land,M.,
            Hauser,L., Kyrpides,N., Lykidis,A., Ivanova,N., Mavrommatis,K.,
            Rubin,E. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2006) US DOE Joint Genome Institute, 2800
            Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP000383.1.
            URL -- http://www.jgi.doe.gov
                   JGI Project ID: 2351473
                   Source DNA and bacteria available from Mark McBride
                   Contacts: Mark McBride (mcbride@uwm.edu)
                             Paul Richardson (microbes@cuba.jgi-psf.org)
            Plasmid and fosmid libraries were prepared at the Joint Genome
            Institute (JGI) Production Genomics Facility (JGI-PGF) in Walnut
            Creek, CA. Shotgun sequencing was performed at the JGI-PGF to a
            coverage of 15x. Large (40 kb), medium (8 kb) and small (3 kb)
            insert random libraries were partially sequenced with average
            success rate of 90% and average high-quality read lengths of 634
            nucleotides. Finishing was performed at JGI-LANL starting with 29
            contigs and 2 scaffolds. Repetitive regions were identified,
            assembled and finished by manually checking paired reads close to
            each repeat in the assembly with consed and then making a
            subassembly for each repetitive region. 27 gaps were closed with
            primer walks and 2 by PCR. Gene predictions were obtained using
            Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis
            of the gene predictions was performed by comparing coding sequences
            against the PFam, BLOCKS and Prodom databases. Gene definitions and
            functional classes were added manually by a team of annotators at
            JGI-LANL, using BLAST results in addition to information from the
            basic analysis. A total of 3793 features have been annotated on the
            sequence record.
             The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/05/2022 08:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,674
            CDSs (with protein)               :: 3,674
            Genes (RNA)                       :: 51
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Cytophaga hutchinsonii ATCC 33406"
                     /mol_type="genomic DNA"
                     /strain="ATCC 33406"
                     /culture_collection="ATCC:33406"
                     /type_material="type strain of Cytophaga hutchinsonii"
                     /db_xref="taxon:269798"
     gene            complement(1..1617)
                     /locus_tag="CHU_RS04470"
                     /old_locus_tag="CHU_0919"
     CDS             complement(1..1617)
                     /locus_tag="CHU_RS04470"
                     /old_locus_tag="CHU_0919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011584315.1"
                     /translation="MHFRLTGILFFLAIFSTAAQSRFSPLERQYLLSEEAQIHSKENN
                     LHTSIQPYFINETKSLFNMDSLCEGVHYTIKRKNFINKVADNFSNNYLVPVDSGIVSI
                     RATPLFDLELSYDPSKKQQYYRNTRGVLIYGDFGQKFSFYTGFYENQASFPGYINTFI
                     DSNLVVPGQGLTKSFKGDKGQDFGLPFGYVCYNPNRYLTVQFGQDKNFIGNGYRSLLR
                     SDNTMNNPFFKLQTSFWHIKYMVLYEQFMDVHAEQITVLGKGYPKKYSTTHYLSWKVS
                     KSFEFGLFESIVWQAADSNGVRGFDWAYLNPVIFLRPTEFYNGSADNALMGINLKYQI
                     LHNMYAYGQIILDDLKFDELKKKNSKYPQKYGFQLGARYFDIAKIKGLAVQGEFNYVM
                     PYTYSHRLSLQNYSHYNQSLAHPQNANFIEWIGIIDYNRKRWSFSNRLVYCNYGADKT
                     AATNYGHNIFADISQYPNYYQGNQIVQGYKNTLIYNTLTAAYIINPKVNMRLEASFTY
                     RSQKNTDENIVDKLIGFSFKTALFNKYYDF"
     gene            complement(1607..2143)
                     /locus_tag="CHU_RS18795"
     CDS             complement(1607..2143)
                     /locus_tag="CHU_RS18795"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="WP_177254166.1"
                     /translation="MLCSWLFPLLAIIIKLSSPGPVFFIQKRSGRNNEEFDCYKFRSM
                     SVNKDSDKVQATVNDSRVTAIGKFLRKSNLDELPQFINVLIGNMSVVGPRPHMLKHTA
                     DYSAIIDKFMVRHLIKPGITGWAQVNGFRGATIQPRYMMKRVRYDLWYIENWTFLLDL
                     QIIVMTAYTMIRGDKNAF"
     gene            complement(2046..2852)
                     /locus_tag="CHU_RS04475"
     CDS             complement(2046..2852)
                     /locus_tag="CHU_RS04475"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_177254165.1"
                     /translation="MSWIILAVSFNIYIYSTVRRRKLELVVWNLIKIVALHVLIIFTF
                     IGALKESYYSRQMLLMTYLILGISLFAWRLLFILFLNWYRKTGSNFRNVLIIGAGPVG
                     LQVMRYIISKDNSGYRFLGFLDDATQHVKHKELVLGNVDALKEICESRFKIDEIFCTL
                     PFTSSKKIRSIIEYGDNHLIRVHLVPDFRGFLNKKVDLEFYDHVPVLNIRKEPLESIF
                     SRMLKRIFDYCIFTFSIDIAVFMVIPITCYYNKIKFTGAGILYTEKKRPK"
     gene            complement(3084..5276)
                     /locus_tag="CHU_RS04480"
                     /old_locus_tag="CHU_0922"
     CDS             complement(3084..5276)
                     /locus_tag="CHU_RS04480"
                     /old_locus_tag="CHU_0922"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="WP_011584316.1"
                     /translation="MKKLFFPCLLFVLIGSMPVLAQVSIVPLTQYNVGETAEYSASDA
                     RTAALTQTDTLKLPYIEDFSGPQLPIDTIKIVAFTSTENIYEIKQLKLHPLLSGDSIR
                     ILNAFGSGEKFDTTAYNILGKRFVKVLDKYTFQLFNDRLLTVPTTVNADPLAQMQYCN
                     WYKLGINGYSTKPDVLGFLDDSGGVYINDNMSLNPVSIGVASFDAIKYTGFPYSNANV
                     NGYADKLTSLPFNLSSYKAKDSVYFSFYWQSKSFGDTPESSAYLILEFKNRNNVWQEV
                     WRKYGDPTQTIDTFRVVNIPLKDTLYRHKGFQYRFRNYGLLSGRFNVWNIDYIYINSK
                     RTVNAAQTGDLCIVNTSRSILTEYTSIPYKHFISLSSSDQADLINQDLFFTLRDLRFG
                     GPALSSKNNLLVRDNLGNTISNDNHADITTGVISRWSFPSPVLDPLKMDTPYVVKQEY
                     SYNIVDYKAPIDLSFNNFKAIETYFYDYYAYDDGIPEDAFEAYQRNGEGILCANKFDI
                     LKEDSLTHIDFCFIKNFGPSVENSIITMAVWKDGTAIEEQLSQQITVKYSTIVNGFVR
                     YAVTPSDKAKLAAGTYYFGFKHFIAGSLFVGYDRNNDNLDKIVTSSSTNTTWTAFNTN
                     ANALTGTLMIRPVFKGKPLLVTDIEDQAASETSNTFILYPNPSAGELHFTGAPEYISI
                     YDLTGKLIQTTHVKETDLLDISALNNGLYVVVLQKADYSETKRLAVQK"
     gene            complement(5293..6069)
                     /locus_tag="CHU_RS04485"
                     /old_locus_tag="CHU_0923"
     CDS             complement(5293..6069)
                     /locus_tag="CHU_RS04485"
                     /old_locus_tag="CHU_0923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="WP_011584317.1"
                     /translation="MKLFKADSLKSVFIHAGIIVSIFVGLILIFFYWYLPSVTKHGEY
                     ISVPKLEGLSISQVEQKLNDLGLRYEISDSTFKPGITPLTVLTQHPLPGNEVKENRRI
                     YLSIAAQHPPNIKMPKLIDESPRGAEMILKSLGLQMGEIKTAPHPYPTVLGQFINGAP
                     VKEGTSIPKGSKIDLLVGTGRGETDVEIPNLVNMSLDEAKSTISSMGLVVGLVNYDTN
                     SKLAEGTVCNQKPAYQSGQKLRSGDIIDLVVAGSDPVIPQ"
     gene            complement(6071..8764)
                     /locus_tag="CHU_RS04490"
                     /old_locus_tag="CHU_0924"
     CDS             complement(6071..8764)
                     /locus_tag="CHU_RS04490"
                     /old_locus_tag="CHU_0924"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_011584318.1"
                     /translation="MRIGIIFGGQSREREISFAGGRTVYDNLNKDIFTTVPIFVDSLG
                     QFILLDWQYIYKGTIRDFYPPFEFLPKSIHGFQVYVESLGDLPKEERDAMIQKVGRKI
                     EPSDLKELIDFAFLALHGPYGEDGAIQGMLDWNNIPYSGSGILPSAIGINKYIQKKLV
                     AQASFNGPNYVTYKKSTWLKGADTKALLAHFESSVGFPFVVKAPSQGSSIGITVISER
                     NEEKLKQALNRSFFLQEITAADWNTKTEHAKIKWVATLTDIRDGIGMPVNDVLQGKTI
                     YHPEELLAHLNQSLSSKERVLLESLDNEEEIIIESFIKGQEFSCIVIQDEDGNPIALP
                     PTGIVKGNDFFDYRSKYLPGMSRKITPIDIPGEQVELIRSECCRLYSTLQFNVYARID
                     GFISPEGEVFLNDPNTTSGMMPSSFFFHQAAEIGLNPSQFLTYIIRTSIQERIKDGKR
                     SLRYKQLLATLDQAISSEKNSSKEKIKIAVVMGGYSSERHISVESGRNIFEKLSSSTK
                     YQPLPVFLTGSDEQHRLFILPINILLKDNADDIREKVINFKGHHPIIEHIAKETESIS
                     SRYVSNAIKDPKEISYKELSELVDGVFIALHGRPGEDGSMQKELEKYQLPYNGSGIQS
                     SQITINKFETNRILREHGISVANHRMVYKQEWLDAGEKLLSEIESGFIYPIIAKPSDD
                     GCSSAVKKIKNRDELRAYTRLMFGEEAIQGSVSSSVLGLKYNEEFPQKDYFLIEDLVS
                     KGNAKHFLEVTGGLMTRFNDKGNVEYEIFEPSESLATGDVLSLEEKFLAGEGQNITPA
                     RYTTNKADYEQVASEVRATLKRVAEVLNIQGYARIDAFVRVFENNRSETIIIEVNSLP
                     GMTPATCIFHQTAINGYKPYDFIDNILTFGIEREKKKLAKI"
ORIGIN      
        1 ctaaaaatcg tagtatttat taaatagcgc tgttttgaaa ctgaatccga taagtttgtc
       61 aacgatattt tcatctgtat ttttctgcga acggtaggta aaggaggctt ctaaacgcat
      121 attaactttg ggattaatga tatatgctgc tgttagcgta ttatatataa gcgtgttttt
      181 gtatccctga acaatctggt tcccctggta ataatttgga tattggctta tatctgcaaa
      241 tatattgtgg ccgtaattcg tggcagctgt tttgtctgcc ccataattac aataaaccag
      301 tctattcgaa aaagaccatc tttttctgtt gtaatcaatg atgcctatcc attcaataaa
      361 attcgcattt tgcggatgcg ccagcgattg attataatga gaatagtttt gtaatgaaag
      421 acggtgcgaa taggtatacg gcataacgta attgaattct ccctgaacag ccagaccttt
      481 gatctttgca atgtcaaaat atcttgcacc taattggaag ccatattttt gagggtattt
      541 agagtttttc ttttttaact catcaaactt caaatcatcc aggataattt gtccatacgc
      601 atacatatta tgcaatatct gatatttcag attgatgccc attaatgcat tatcagcgga
      661 gccattataa aactcagtag gccgtaaaaa aattacagga tttaagtatg cccagtcaaa
      721 tccgcgtaca ccatttgaat cagcggcttg ccagacaata gattcaaaaa gcccaaattc
      781 aaatgatttt gatactttcc agcttaaata atgagtcgtg gaatattttt tcggatagcc
      841 tttccctaaa acagtaatct gttcggcatg cacatccata aattgttcat acaaaaccat
      901 gtatttaata tgccagaaag atgtttgaag tttaaaaaaa ggattattca tcgtgttgtc
      961 agaccgtaat aaggaacggt atccattacc aataaagttt ttgtcctgcc cgaattgtac
     1021 cgtcaggtat ctgttgggat tatagcaaac ataaccaaaa ggcaaaccaa aatcctgtcc
     1081 tttatctcct ttaaagcttt ttgttaatcc ctgtccggga actaccagat tgctatcaat
     1141 gaatgtattt atgtatccgg ggaacgaggc ctggttttca taaaagcctg tatagaatga
     1201 aaacttttgt ccaaaatccc catagattaa aacaccacgg gtatttctat aatattgctg
     1261 ttttttgctg gggtcatatg ataattctaa atcaaacagg ggagtagcac ggatagaaac
     1321 aatgcccgaa tctacaggaa ccagataatt attgctgaaa ttatctgcga ctttatttat
     1381 aaaatttttt ctttttatag tataatgaac accctcgcac agactatcca tattgaataa
     1441 tgattttgtt tcattaataa aatatggctg gatagatgtg tgcagattgt tttctttaga
     1501 atgaatctgc gcctcttcac ttaacagata ttgtcgctca agcggactga acctgctttg
     1561 cgctgccgtt gaaaaaatgg caaggaaaaa aagtataccc gttagtctaa aatgcatttt
     1621 tgtcgcctcg tatcattgta taagcagtca ttactataat ttgcaggtcc agcaaaaagg
     1681 tccagttttc aatataccat aaatcgtaac gaacacgctt catcatatat ctcggttgga
     1741 tggtagcacc tctaaagcca tttacctgtg cccagcctgt tatgccgggt ttaattaaat
     1801 gcctaaccat aaatttatca ataatagcag aataatcagc cgtatgtttc agcatgtgcg
     1861 gacgcggccc gacaacagac atattcccga tcaatacatt tataaactgt ggcagttcat
     1921 ccagatttga ttttcggaga aatttaccaa tagcagttac ccgtgaatca tttaccgttg
     1981 cctgaacttt atcagaatct ttattcacac tcatgcttct gaatttataa caatcaaatt
     2041 cttcattatt tcggccgctt cttttctgta taaagaatac cggccccggt gaacttaatt
     2101 ttattataat agcaagtaat gggaataacc atgaacacag caatatcaat actaaaagtg
     2161 aaaatacaat aatcaaatat ccgttttaac attcggctaa agatactttc taacggttct
     2221 ttccgaatgt ttaataccgg tacatgatca taaaattcaa gatctacttt tttatttaaa
     2281 aagcctctga agtcaggaac caggtgcaca cggatcaaat ggttatcgcc atattcaata
     2341 atggaacgga tttttttaga cgatgtaaat ggaagtgtac agaagatctc atcaatttta
     2401 aacctgcttt cacaaatttc ttttaacgca tcaacattac ctaaaaccaa ttctttgtgt
     2461 tttacatgtt gcgtggcatc atccagaaat cctaaaaatc tatatcccga attgtctttc
     2521 gaaatgatat agcgcattac ctgtaaacca accggtcctg caccaataat caatacattt
     2581 ctaaaattag aaccagtttt acggtaccaa ttaaggaaca gaataaaaag cagacgccat
     2641 gcaaaaagac tgatgcctaa aatcagatac gtcattaaga gcatttgtct ggaataatac
     2701 gactctttta aggcgccgat aaaagtaaat ataataagta catgcaaggc aacaattttt
     2761 atcagattcc atacaactaa ttctaatttc cgccgccgga cagttgaata tatatagatg
     2821 ttaaagctaa ccgcaagaat aatccacgac atattaaaaa taatatgcag ggtaatatag
     2881 tggtccgtaa tgtgggaaaa aggtggtgtt tgatcagaaa ataaatagga aataaaaaat
     2941 gcgatattta ataatgatac atctcctata aattgtatta accataagaa cttactgtat
     3001 ctggaattca taattttgta ttggtaattt acatgtagac gaaattacga aaagtaatta
     3061 catcttttga attgagaata attttatttt tgaacggcaa gacgtttagt ttctgaataa
     3121 tcggcctttt gcagaactac tacatataaa ccattgttca gcgcggaaat atcaagcaaa
     3181 tctgtttctt tcacatgagt tgtttgaatt aatttacccg ttaagtcata aatcgatata
     3241 tattcaggtg caccggtaaa atgtaactct ccggcagacg gattgggata aagaataaat
     3301 gtattggatg tttcggatgc tgcttgatct tcaatatcag taacaagcaa tggttttcct
     3361 ttaaataccg gtcttatcat caacgtacct gttaacgcat tcgcatttgt attgaaagca
     3421 gtccaggtag tattcgtact gctgctggta actattttat ccaggttatc attatttcta
     3481 tcataaccta caaataagct tccggcaata aaatgtttaa aaccaaaata ataggtgccc
     3541 gcagctaact tcgctttatc tgaaggtgtt actgcataac gtacaaatcc attaacaatc
     3601 gtgctgtatt ttacggttat ttgctgactt agttgttctt caatggctgt tccatctttc
     3661 caaacagcca ttgtaatgat tgagttttcc acactggggc cgaagttttt gataaagcag
     3721 aagtctatgt gcgtaagcga atcctctttt aaaatgtcga atttattggc gcataagatt
     3781 ccttcaccgt ttcgctgata tgcttcaaat gcatcttccg gtatgccgtc atcataggca
     3841 tagtaatcgt agaaatatgt ttctatggcc ttaaagttat tgaaagataa atcaatcgga
     3901 gctttataat ctacaatgtt gtaggaatac tcttgtttta cgacatatgg agtatccatt
     3961 tttagaggat ctaataccgg agaagggaaa gaccatcttg atataacacc cgtagttata
     4021 tcagcatgat tatcattcga aatagtattg cctaaattat cccggacaag aagattgttt
     4081 ttagaagata gggcagggcc gccaaatctt aaatctctta atgtgaaaaa aagatcctga
     4141 ttgatgagat ctgcttgatc agatgaggac aatgaaatga aatgtttata aggtatagat
     4201 gtgtactccg taagaatact tctgcttgta tttacaatac acagatctcc tgtttgagct
     4261 gcatttaccg tgcgttttga attgatataa atgtaatcga tgttccacac attaaaccgt
     4321 ccgcttaaca aaccataatt tctgaaccgg tattgaaacc ctttgtgccg gtaaagagtg
     4381 tcttttaaag gtatattaac aacacggaag gtatcaattg tttgtgtcgg gtcgccatat
     4441 ttacgccata cttcctgcca tacgttattg cggtttttaa attccagaat taaataggca
     4501 gaactttccg gtgtatctcc gaagcttttt gattgccagt aaaagctgaa ataaacagag
     4561 tcttttgctt tataggaaga taaattaaag ggcaatgagg ttaatttatc agcatacccg
     4621 ttaacattag catttgaata cggaaatccc gtatacttta tcgcatcaaa agaggcaacc
     4681 ccgatgctta caggatttaa agacatgttg tcattaatat acacaccacc gctatcgtca
     4741 agaaaaccta acacatcggg ttttgtactg tagccattaa tacccagttt ataccagttg
     4801 caatattgca tctgagctaa cgggtcagca ttaacagtgg ttggaaccgt taaaagcctg
     4861 tcattaaata gctgaaaggt atatttatca agaactttta caaatcgttt acctaaaata
     4921 ttataagctg tagtatcaaa tttttccccg cttccgaatg catttaaaat acggatacta
     4981 tctccgctaa gcagcggatg aagctttaat tgtttgatct cgtatatatt ttctgttgaa
     5041 gtaaaggcaa ctattttaat ggtatcaata ggtaattgtg gccctgaaaa atcttcaata
     5101 tagggaagtt ttaacgtatc tgtttgagtg agtgctgcag tacgtgcatc ggaggctgaa
     5161 tattcagctg tttcaccaac attatattgg gtcaatggta caatggatac ctgtgccaat
     5221 acaggcatag aaccaatcaa cacaaaaagc agacacggaa aaaaaagttt tttcatacgc
     5281 tggataaatg aattattgag gaattaccgg atcgcttccc gctacaacaa gatcgattat
     5341 atctccagaa cgaagttttt gccccgattg gtatgcaggt ttttgattgc atactgttcc
     5401 ttctgctaat tttgaatttg tatcataatt aacaagccca accaccaggc ccatactgct
     5461 gatggtggat ttcgcctcat ctaaagacat atttaccaaa ttgggaattt caacatccgt
     5521 ttcacctctg ccggtaccta ccaacaggtc aattttagag cctttgggaa tagaagtacc
     5581 ttcttttacg ggagcaccat ttataaactg ccctaaaaca gtagggtatg gatgtggagc
     5641 tgttttaatt tcgcccatct gtaaacctag gcttttaaga atcatttcag ctcctctggg
     5701 agattcatca atcagtttag gcattttaat attgggcggg tgctgagctg caatactcaa
     5761 atagatcctt ctgttttctt ttacttcatt gcccggcagc ggatgttgtg ttaaaacagt
     5821 taatggtgta atacccggct taaatgttga atcactgatt tcataacgca aacccaggtc
     5881 atttaatttc tgttcaacct ggctgatcga caggccttct aatttaggta cactgatgta
     5941 ttctccgtgc ttggttacag acggtaaata ccagtaaaag aagatgagta ttaaacctac
     6001 aaaaatactt actataattc ctgcatgaat gaatacactt tttaatgaat ctgctttaaa
     6061 taatttcata ttatatttta gctaattttt tcttttctct ttctatgcca aatgttaaaa
     6121 tgttatcaat gaagtcgtat ggcttatatc cattaatagc cgtctggtga aatatacagg
     6181 ttgcaggcgt catgcccggc agcgagttta cttcaatgat aatggtttca gatctattgt
     6241 tttcaaagac acgtacaaac gcatcgatcc gtgcataccc ctgaatattc aatacttcag
     6301 ctacgcgttt taatgttgca cgtacttctg aagccacttg ctcataatct gctttgttgg
     6361 ttgtataacg cgccggggtt atgttctgtc cttcaccggc aaggaatttt tcttccaggg
     6421 ataaaacatc accggtagcc aacgattcag aaggttcaaa tatttcatat tcaacgttgc
     6481 ctttatcgtt gaaacgggtc attaatccgc ccgtaacttc caggaaatgt tttgcattgc
     6541 ctttagaaac cagatcttca attaagaaat aatctttctg cggaaattct tcgttatatt
     6601 tcaatcccaa tacggaagaa ctgacagatc cctgaatggc ttcttcacca aacattaatc
     6661 gggtataggc acgaagctcg tctctgtttt ttatcttttt aaccgcagag ctgcatccat
     6721 cgtcagatgg tttagcaatg atgggatata taaaaccgga ttcaatttcg gataacaatt
     6781 tttcgcctgc gtctaaccat tcctgcttgt aaaccattct gtggtttgca acagaaatac
     6841 catgctcccg aagaatgcgg tttgtctcaa atttattgat cgtgatctga gaactttgta
     6901 tacctgaacc gttatacggc aactgatatt tctcaagctc tttctgcatg ctgccatctt
     6961 ctcccggccg gccgtgcagg gcaatgaaga cgccatctac cagctcagaa agttctttgt
     7021 aggaaatctc tttcggatct ttaattgcat tgctcacata ccggctgctg atcgattccg
     7081 tttcttttgc aatatgttca atgatcgggt ggtgcccttt aaagttgatt actttttcac
     7141 gtatatcatc tgcattgtct ttcaataaaa tattgatcgg taagatgaat aaacggtgct
     7201 gctcgtcact gcctgttaag aacacaggaa gcggctggta ttttgtactg gaggaaagtt
     7261 tttcaaagat atttcttccg ctttcaacgg agatgtggcg ttcagaagaa tagccaccca
     7321 ttaccacagc aattttaatt ttttctttgg aagaattctt ttcagatgaa attgcctgat
     7381 ccagtgttgc cagcaattgt ttgtagcgca aacttctttt gccgtcttta atacgctcct
     7441 gaatagacgt acggataatg taggtaagga attgagacgg atttaagccg atctctgctg
     7501 cctgatgaaa aaagaaggac gatggcatca taccagaagt cgtattcggg tcgtttaaga
     7561 agacctctcc ttcaggagaa ataaaaccat caatacgtgc atatacattg aactgtaagg
     7621 ttgaatataa gcggcagcat tcgctgcgga tcaattcaac ttgttcgcca ggaatatcaa
     7681 taggtgtgat ctttcttgac atgcccggca gatattttga acggtaatca aagaaatcat
     7741 ttcctttaac aatacccgta ggaggcagcg ctattggatt accatcttca tcctgtataa
     7801 caatgcagga gaattcctgg cctttaataa aggattcaat gatgatttct tcctcattat
     7861 ctaaactttc caataaaaca cgttctttag aagaaagcga ttgatttaaa tgtgctaata
     7921 attcttccgg atgataaatg gttttgcctt gtaatacatc atttacgggc atgccaatac
     7981 catcgcggat atcggtaagg gttgccaccc attttatttt cgcatgttct gtttttgtgt
     8041 tccagtctgc agctgtaatt tcctgaagaa aaaagctacg gtttagcgct tgctttaatt
     8101 tttcttcatt tcgctcactg ataaccgtaa taccaatgga agagccttgt gacggtgctt
     8161 ttacaacaaa aggaaaacct acgcttgatt caaaatgtgc aagcagtgct tttgtatcag
     8221 caccttttag ccaggtagat tttttgtagg taacatagtt aggtccgttg aaactggctt
     8281 gtgctactaa tttcttctga atgtatttat tgataccaat agctgaaggc agaatgcctg
     8341 aacctgaata gggaatatta ttccagtcca gcataccttg tatggcacca tcttctccat
     8401 aaggaccatg taaagcaagg aaggcaaagt caattaattc tttcaggtca gaaggctcaa
     8461 tttttctgcc tactttttga atcatggcat cccgttcttc ttttggaagg tcaccaagtg
     8521 attcaacata gacctgaaag ccatgaatgc ttttaggaag aaattcaaaa ggagggtaga
     8581 aatcacgaat ggttcccttg tagatatatt gccagtctaa taaaatgaac tggccaaggc
     8641 tatcaacaaa gatgggaacg gtagtgaaaa tatctttatt taaattatcg taaacagttc
     8701 ttcctccagc aaatgaaatt tctcgttctc tagactgtcc accaaagatg attcctattc
     8761 tcat
//
