Basic Information | |
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Species | Ricinus communis |
Cazyme ID | 28493.m000029 |
Family | GH43 |
Protein Properties | Length: 333 Molecular Weight: 37670.9 Isoelectric Point: 7.0261 |
Chromosome | Chromosome/Scaffold: 28493 Start: 915 End: 1916 |
Description | |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH43 | 27 | 314 | 4.2e-28 |
YRDPVFDGAADVSIVYDRAAKLWKMFYTNRRATMKLPDPKDVEWVHGTAIGIAASDDGVHWRYQGTAEFPKACTDVTLWAPEIYYENGTYHMWLTIVPGI FHRWGGPGADGRIEHLTSTDLSTWKCEGTVKLDTGRMIDPTVIKLGQGYRMWYKDERAGSKILAADSQDLRTWKKVSDEPVNPTTGEGPKVFRFKGYYWM VVDVWKGLMVLRSDDARTWTEQKGYILGEPGHKATDRAKGQHADVVVDGDRAFIYYFVHQENEPEAATDARWHQRTVIQVAELVFKDG |
Full Sequence |
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Protein Sequence Length: 333 Download |
MKSILAALAL ALPLMASAAD VAPKPVYRDP VFDGAADVSI VYDRAAKLWK MFYTNRRATM 60 KLPDPKDVEW VHGTAIGIAA SDDGVHWRYQ GTAEFPKACT DVTLWAPEIY YENGTYHMWL 120 TIVPGIFHRW GGPGADGRIE HLTSTDLSTW KCEGTVKLDT GRMIDPTVIK LGQGYRMWYK 180 DERAGSKILA ADSQDLRTWK KVSDEPVNPT TGEGPKVFRF KGYYWMVVDV WKGLMVLRSD 240 DARTWTEQKG YILGEPGHKA TDRAKGQHAD VVVDGDRAFI YYFVHQENEP EAATDARWHQ 300 RTVIQVAELV FKDGRLEVDR DRDVSFRLKA PSL |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG3507 | XynB | 1.0e-7 | 39 | 290 | 295 | + Beta-xylosidase [Carbohydrate transport and metabolism] | ||
cd08979 | GH_J | 4.0e-8 | 33 | 199 | 182 | + Glycosyl hydrolase families 32 and 68, which for the clan GH-J. This glycosyl hydrolase family clan J (according to carbohydrate-active enzymes database (CAZY)) includes family 32 (GH32) and 68 (GH68). The overall sequence homology between the two families is low (<15% identity), but common sequence motifs have been identified. GH32 enzymes are invertases that also include other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). GH32 enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
cd08979 | GH_J | 3.0e-12 | 37 | 228 | 222 | + Glycosyl hydrolase families 32 and 68, which for the clan GH-J. This glycosyl hydrolase family clan J (according to carbohydrate-active enzymes database (CAZY)) includes family 32 (GH32) and 68 (GH68). The overall sequence homology between the two families is low (<15% identity), but common sequence motifs have been identified. GH32 enzymes are invertases that also include other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). GH32 enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
cd08978 | GH_F | 2.0e-40 | 36 | 308 | 303 | + Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
cd08984 | GH43_5 | 1.0e-148 | 25 | 320 | 302 | + Glycosyl hydrolase family 43. This glycosyl hydrolase family 43 (GH43) includes enzymes with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_002535874.1 | 0 | 1 | 333 | 1 | 333 | conserved hypothetical protein [Ricinus communis] |
RefSeq | ZP_01302746.1 | 0 | 5 | 328 | 1 | 321 | hypothetical protein SKA58_03620 [Sphingomonas sp. SKA58] |
RefSeq | ZP_04770435.1 | 0 | 14 | 331 | 21 | 334 | Glycosyl hydrolase family 32 domain protein [Asticcacaulis excentricus CB 48] |
RefSeq | ZP_05059045.1 | 0 | 10 | 328 | 327 | 647 | glycosyl hydrolase, family 43 [Verrucomicrobiae bacterium DG1235] |
RefSeq | ZP_06172071.1 | 0 | 26 | 331 | 20 | 320 | Glycosyl hydrolase family 32 domain protein [Brevundimonas subvibrioides ATCC 15264] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1wl7_A | 0.005 | 73 | 228 | 42 | 215 | A Chain A, Structure Of The Thermostable Arabinanase |
Signal Peptide | ||||
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Cleavage Site | ||||
18 |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EX976536 | 148 | 25 | 153 | 0.00000000004 |
FG121500 | 80 | 81 | 160 | 2.1 |