Basic Information | |
---|---|
Species | Selaginella moellendorffii |
Cazyme ID | 79855 |
Family | GH31 |
Protein Properties | Length: 872 Molecular Weight: 97312.4 Isoelectric Point: 6.4756 |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH31 | 233 | 726 | 0 |
FYFFAGPSPLSVVQQYTAHVGKPAAMPYWSLGFHQCRWGYKNVSQLEHVVAEYKKANLPLEVMWNDIDHMDVYKDFTLDPVNYPAEQLRAFVEKLHKNGQ RYVLIVDPGLKPEKNYETYRRAKEMDVFIKDVQGKPYLGQVWPGPVHFPDFLHPRALEFWTGEVSRFHKEVPFDGLWIDMNEASNFCQGVTCTLPANVTC PIPGSFTQCCLVCSNDLATKWDNPPYAINNAGTHRSLGGKTIPTSATHYNGTLEYNAHNLYGLAEAIVINSALKTVVKKRPFVLSRSTFAGSGRVTAHWL GDNRASWNDLKYSISGILGAGLAGIPMVGADICGFSGNTTEELCNRWIQLGAFYPFSRDHNDFFSSPQELFVWKSVTRSARKALELRYKLLPYLYTLSFE AHTLGSPVARPLFFTFPNDRATLDIDKQFLLGRGILVSPVLTPNATTVKAYFPQGTWYSLFDYTKYVTSPSGSYQQLDAPWDTINVHVHEGSII |
Full Sequence |
---|
Protein Sequence Length: 872 Download |
MIAAVSREYR VLSVIEQANA GGGSAILVEI ADRIDDFGPD IEHLLISVSY ETDERLHLMI 60 TDADSPRWEI PFKLIPRSMD GNSSSARFSQ EVRKITSPKL QLSYTVNPFS FTVTRVSNGE 120 ILFDSPSSSR FVFKDQYLEI STRIPAQAAL YGLGESTRSD GFRILPNSTY TLWAADTGAD 180 NTDVNLYGSH PFYMDVRSGG QAYGVLLLNS NGMDVNYEGE FLTYKVLGGV FDFYFFAGPS 240 PLSVVQQYTA HVGKPAAMPY WSLGFHQCRW GYKNVSQLEH VVAEYKKANL PLEVMWNDID 300 HMDVYKDFTL DPVNYPAEQL RAFVEKLHKN GQRYVLIVDP GLKPEKNYET YRRAKEMDVF 360 IKDVQGKPYL GQVWPGPVHF PDFLHPRALE FWTGEVSRFH KEVPFDGLWI DMNEASNFCQ 420 GVTCTLPANV TCPIPGSFTQ CCLVCSNDLA TKWDNPPYAI NNAGTHRSLG GKTIPTSATH 480 YNGTLEYNAH NLYGLAEAIV INSALKTVVK KRPFVLSRST FAGSGRVTAH WLGDNRASWN 540 DLKYSISGIL GAGLAGIPMV GADICGFSGN TTEELCNRWI QLGAFYPFSR DHNDFFSSPQ 600 ELFVWKSVTR SARKALELRY KLLPYLYTLS FEAHTLGSPV ARPLFFTFPN DRATLDIDKQ 660 FLLGRGILVS PVLTPNATTV KAYFPQGTWY SLFDYTKYVT SPSGSYQQLD APWDTINVHV 720 HEGSIIPMQE FALTTRLARK TPFTLVVAFS STDSENSTAS GELFLDDDDA LEMKVAEGKS 780 SFIKFAASSI GNRLSVKSRV SYGEFALQQK WIISKVVVLG LGSFALDKQQ QELVKGKILL 840 PQSADHVEEV NELNLPLGKP FHISLKMASY Q* |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06602 | GH31_MGAM_SI_GAA | 5.0e-90 | 487 | 655 | 170 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
cd06602 | GH31_MGAM_SI_GAA | 3.0e-93 | 253 | 418 | 169 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
cd06604 | GH31_glucosidase_II_MalA | 2.0e-107 | 253 | 637 | 389 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
COG1501 | COG1501 | 9.0e-133 | 29 | 800 | 784 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
pfam01055 | Glyco_hydro_31 | 0 | 234 | 726 | 497 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAL40352.1 | 0 | 5 | 864 | 28 | 907 | AF448201_1 putative alpha-xylosidase [Pinus pinaster] |
GenBank | ABR16166.1 | 0 | 9 | 869 | 29 | 908 | unknown [Picea sitchensis] |
RefSeq | XP_001753937.1 | 0 | 9 | 823 | 9 | 827 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001766654.1 | 0 | 10 | 863 | 34 | 889 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001769951.1 | 0 | 5 | 820 | 29 | 844 | predicted protein [Physcomitrella patens subsp. patens] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 4 | 866 | 40 | 907 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
PDB | 3w37_A | 0 | 4 | 866 | 40 | 907 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
PDB | 3ctt_A | 0 | 37 | 820 | 78 | 820 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
PDB | 2qmj_A | 0 | 37 | 820 | 78 | 820 | X Chain X, Crystal Structure Of The Full-Length Autotransporter Esta From Pseudomonas Aeruginosa |
PDB | 2qly_A | 0 | 37 | 820 | 78 | 820 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |
Metabolic Pathways | |||
---|---|---|---|
Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
HO795307 | 671 | 217 | 868 | 0 |
HO777477 | 525 | 5 | 515 | 0 |
FE500728 | 248 | 388 | 635 | 0 |
FE513699 | 248 | 498 | 745 | 0 |
DR736759 | 362 | 230 | 591 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|