Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10001266m |
Family | GH13 |
Protein Properties | Length: 425 Molecular Weight: 46914.9 Isoelectric Point: 5.2672 |
Chromosome | Chromosome/Scaffold: 5 Start: 40192464 End: 40194246 |
Description | alpha-amylase-like |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 33 | 343 | 1.4013e-45 |
ESSNKAGGWYNSLKNSIPDLSNAGITHVWLPPPSQSVAPQGYMPGRLYDLDASKYGSQADLKSLIQAFRQKGIKCLADMVINHRTAERKDGRGIYCIFEG GTSDDRLDWGPSFICRGDKEYSDGQGNDDTGEDFQPAPDIDHLNPRVQKELSDWMNWLKTEIGFDGWRFDFVKGYAPSITKVYMENTSPDFAVGEKWDSL SYGPDGKPDANQDGHRGALKDWVQAAGGAVAAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGILPQNAVTFIDNHDTGSTQRLWPFPSDKVMLGYAYI LTHPGTPCIFY |
Full Sequence |
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Protein Sequence Length: 425 Download |
MSTLKSFCFL SFLLAIFLPF TSPALLFQGF NWESSNKAGG WYNSLKNSIP DLSNAGITHV 60 WLPPPSQSVA PQGYMPGRLY DLDASKYGSQ ADLKSLIQAF RQKGIKCLAD MVINHRTAER 120 KDGRGIYCIF EGGTSDDRLD WGPSFICRGD KEYSDGQGND DTGEDFQPAP DIDHLNPRVQ 180 KELSDWMNWL KTEIGFDGWR FDFVKGYAPS ITKVYMENTS PDFAVGEKWD SLSYGPDGKP 240 DANQDGHRGA LKDWVQAAGG AVAAFDFTTK GILQAAVQGE LWRLKDSNGK PPGFIGILPQ 300 NAVTFIDNHD TGSTQRLWPF PSDKVMLGYA YILTHPGTPC IFYDHFFDWG LKEAISKLAA 360 VRNRNGINTA SRVNILASDA DVYIAAIGDR VIMKIGPKMD IGNLIPSDFK VAADGTDYAV 420 WEKN* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 2.0e-52 | 26 | 364 | 411 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN02784 | PLN02784 | 3.0e-145 | 25 | 423 | 400 | + alpha-amylase | ||
PLN02361 | PLN02361 | 5.0e-149 | 25 | 423 | 406 | + alpha-amylase | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-164 | 26 | 373 | 351 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN00196 | PLN00196 | 0 | 5 | 424 | 425 | + alpha-amylase; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAC76729.1 | 0 | 1 | 422 | 4 | 421 | alpha-amylase [Vigna angularis] |
Swiss-Prot | P17859 | 0 | 1 | 422 | 4 | 421 | AMYA_VIGMU RecName: Full=Alpha-amylase; AltName: Full=1,4-alpha-D-glucan glucanohydrolase; Flags: Precursor |
RefSeq | XP_002301187.1 | 0 | 24 | 423 | 4 | 403 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002327139.1 | 0 | 3 | 423 | 2 | 423 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002510218.1 | 0 | 7 | 423 | 1 | 420 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1bg9_A | 0 | 25 | 423 | 2 | 402 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
PDB | 1ava_B | 0 | 25 | 423 | 2 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1ava_A | 0 | 25 | 423 | 2 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1amy_A | 0 | 25 | 423 | 2 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 2qpu_C | 0 | 25 | 424 | 3 | 405 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |