| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G123633_T01 |
| Family | GH32 |
| Protein Properties | Length: 591 Molecular Weight: 66666.6 Isoelectric Point: 9.028 |
| Chromosome | Chromosome/Scaffold: 10 Start: 114288173 End: 114290565 |
| Description | cell wall invertase 2 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GH32 | 50 | 376 | 0 |
| HFQPPKHWINDPNGPMYYKGLYHLFYQYNPKGAVWGNIVWAHSVSTDLIDWTALAPGIYPSRQFDVKGCWSGSATVLPSGVPVVMYTGIDPDEHQVQNVA YPKNLSDPFLREWVKPDYNPIIAPDSGVNASAFRDPTTAWYGPDRHWRLLVGSKVGGKGLAVLYRSRDFRRWVKAHHPLHSGLTGMWECPDFFPVAVHGG GRHYRRGVDTAELHDRALAEEVKYVLKVSLDLTRYEYYTVGTYDHATDRYTPDAGFRDNDYGLRYDYGDFYASKSFYDPAKRRRVLWGWANESDTVPDDR RKGWAGIQAIPRKLWLSPRGKQLIQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 591 Download |
| MGMAALLLVW ALLHCTGTAV TMVRASHAVY PELQSLEAEK VDETSRTGYH FQPPKHWIND 60 PNGPMYYKGL YHLFYQYNPK GAVWGNIVWA HSVSTDLIDW TALAPGIYPS RQFDVKGCWS 120 GSATVLPSGV PVVMYTGIDP DEHQVQNVAY PKNLSDPFLR EWVKPDYNPI IAPDSGVNAS 180 AFRDPTTAWY GPDRHWRLLV GSKVGGKGLA VLYRSRDFRR WVKAHHPLHS GLTGMWECPD 240 FFPVAVHGGG RHYRRGVDTA ELHDRALAEE VKYVLKVSLD LTRYEYYTVG TYDHATDRYT 300 PDAGFRDNDY GLRYDYGDFY ASKSFYDPAK RRRVLWGWAN ESDTVPDDRR KGWAGIQAIP 360 RKLWLSPRGK QLIQWPVEEV KALRAKHVNV SDKVVKSGQY FEVTGFKSVQ SDVEVEFAIG 420 DLSKAERFNP RWLTDPQALC KKRGARETGE VGPFGLWVLA AGDLTERTAV FFRVFRANSN 480 SSSSRLVVLM CNDPTNSTFE AQVYRPTFAS FVNVDIARTK TIALRTLIDH SVVESFGAGG 540 RTCILTRVYP KKALGDNAHL FVFNHGEVDV KVTRLDAWEM RTPKMNAPAQ * 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-46 | 46 | 390 | 357 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-60 | 18 | 403 | 396 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-86 | 56 | 379 | 337 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-143 | 50 | 376 | 336 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 4.0e-175 | 50 | 540 | 501 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAQ24870.1 | 0 | 30 | 587 | 31 | 584 | cell wall invertase 3 [Oryza sativa Indica Group] |
| EMBL | CAD40590.2 | 0 | 30 | 587 | 31 | 581 | OJ000126_13.7 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001052747.1 | 0 | 30 | 587 | 31 | 584 | Os04g0413200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001104898.1 | 0 | 1 | 590 | 1 | 586 | invertase cell wall3 [Zea mays] |
| RefSeq | XP_002489109.1 | 0 | 14 | 590 | 28 | 598 | hypothetical protein SORBIDRAFT_0067s002010 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 41 | 586 | 4 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 42 | 586 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 42 | 586 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 42 | 586 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 42 | 586 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
