PUL ID

PUL0004

PubMed

26827771, Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15.

Characterization method

enzyme activity assay,substrate binding assay

Genomic accession number

KM624528.1

Nucelotide position range

1-3311

Substrate

glucose,cellobiose,maltose

Loci

glu1392,glu1923

Species

uncultured bacterium/77133

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

bgl1391 - AKN80695.1 18 - 1923 (+) KM624528.1:19-1924 -
bgl1392 - AKN80694.1 1919 - 3311 (+) KM624528.1:1920-3312 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 19 - 1923 (+) TC: gnl|TC-DB|P08722|4.A.1.2.2 Yes
- 1920 - 3311 (+) CAZyme: GH1 Yes

PUL ID

PUL0004

PubMed

26827771, Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15.

Title

A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase.

Author

Gao G, Wang A, Gong BL, Li QQ, Liu YH, He ZM, Li G

Abstract

Functional screening of a metagenomic library of termite hindgut identified an overlapping gene cluster encoding the phosphotransferase system (PTS) components, which consisted of a glucoside specific PTS enzyme II gene (glu1923) and a glycoside hydrolase gene (glu1392). Hydrolytic experiments revealed that the combined effect of Glu1923 and Glu1392 was responsible for the utilization of glucosidic substrates in recombinant Escherichia coli (E. coli) strains. Yeast two hybrid analysis suggested that there was an interaction between Glu1923 and Glu1392, and the domain EIIA of Glu1923 played an important role for the interaction. In addition, the hydrolase Glu1392 displayed hydrolysis ability toward cellobiose and maltose, and had a very high tolerance to glucose with a Ki value of 2.25M. These properties make Glu1392 an interesting candidate in biotechnology applications after further study.