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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0025

PubMed

32093600, Biochemistry (Mosc). 2020 Feb;85(2):241-247. doi: 10.1134/S000629792002011X.

Characterization method

sugar utilization assay,NMR,sequence homology analysis

Genomic accession number

KC526897.2

Nucelotide position range

1-21226

Substrate

capsule polysaccharide

Loci

QDM55371.1-AHB32294.2

Species

Acinetobacter baumannii/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzc - QDM55371.1 0 - 2187 (-) KC526897.2:1-2188 -
wzb - QDM55372.1 2206 - 2635 (-) KC526897.2:2207-2636 -
wza - QDM55373.1 2639 - 3758 (-) KC526897.2:2640-3759 -
gna - AHB32279.1 4094 - 5369 (+) KC526897.2:4095-5370 -
wzx - AHB32280.1 5370 - 6633 (+) KC526897.2:5371-6634 -
gtr67 - AHB32281.1 6634 - 7546 (+) KC526897.2:6635-7547 -
gtr68 - AHB32282.2 7542 - 8652 (+) KC526897.2:7543-8653 -
wzy - AHB32283.1 8648 - 9746 (+) KC526897.2:8649-9747 -
gtr69 - GT0|GT2 9742 - 10513 (+) KC526897.2:9743-10514 -
gtr70 - AHB32285.1 10509 - 11283 (+) KC526897.2:10510-11284 -
ugd3 - AHB32286.1 11301 - 12474 (+) KC526897.2:11302-12475 -
atr9 - AHB32287.2 12501 - 12879 (-) KC526897.2:12502-12880 -
orf - AHB32288.2 13154 - 14018 (+) KC526897.2:13155-14019 -
itrA2 - AHB32289.2 14195 - 14858 (+) KC526897.2:14196-14859 -
galU - AHB32290.1 14882 - 15758 (+) KC526897.2:14883-15759 -
ugd - AHB32291.1 15873 - 17136 (+) KC526897.2:15874-17137 -
gpi - AHB32292.1 17132 - 18803 (+) KC526897.2:17133-18804 -
gne1 - AHB32293.1 18795 - 19812 (+) KC526897.2:18796-19813 -
pgm - AHB32294.2 19855 - 21226 (-) KC526897.2:19856-21227 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2187 (-) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 2207 - 2635 (-) other Yes
- 2640 - 3758 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 4095 - 5369 (+) other Yes
- 5371 - 6633 (+) other Yes
- 6635 - 7546 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 7543 - 8652 (+) CAZyme: GT4 Yes
- 8649 - 9746 (+) other Yes
- 9743 - 10513 (+) CAZyme: GT0|GT2_Glycos_transf_2 Yes
- 10510 - 11283 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 11302 - 12474 (+) other Yes
- 12502 - 12879 (-) other Yes
- 13155 - 14018 (+) other Yes
- 14196 - 14858 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 14883 - 15758 (+) CDS No
- 15874 - 17136 (+) CDS No
- 17133 - 18803 (+) CDS No
- 18796 - 19812 (+) CDS No
- 19856 - 21226 (-) CDS No

PUL ID

PUL0025

PubMed

32093600, Biochemistry (Mosc). 2020 Feb;85(2):241-247. doi: 10.1134/S000629792002011X.

Title

Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549.

Author

Cahill SM, Arbatsky NP, Shashkov AS, Shneider MM, Popova AV, Hall RM, Kenyon JJ, Knirel YA

Abstract

Capsular polysaccharide (CPS), isolated from Acinetobacter baumannii LUH5549 carrying the KL32 capsule biosynthesis gene cluster, was studied by sugar analysis, Smith degradation, and one- and two-dimensional (1)H and (13)C NMR spectroscopy. The K32 CPS was found to be composed of branched pentasaccharide repeats (K units) containing two residues of beta-D-GalpNAc and one residue of beta-D-GlcpA (beta-D-glucuronic acid) in the main chain and one residue each of beta-D-Glcp and alpha-D-GlcpNAc in the disaccharide side chain. Consistent with the established CPS structure, the KL32 gene cluster includes genes for a UDP-glucose 6-dehydrogenase (Ugd3) responsible for D-GlcA synthesis and four glycosyltransferases that were assigned to specific linkages. Genes encoding an acetyltransferase and an unknown protein product were not involved in CPS biosynthesis. Whilst the KL32 gene cluster has previously been found in the global clone 2 (GC2) lineage, LUH5549 belongs to the sequence type ST354, thus demonstrating horizontal gene transfer between these lineages.