Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL ID

PUL0058

PubMed

8145641, Mol Microbiol. 1994 Jan;11(1):203-18. doi: 10.1111/j.1365-2958.1994.tb00301.x.

Characterization method

enzyme activity assay

Genomic accession number

Z25795.1

Nucelotide position range

463-8375

Substrate

glycogen

Loci

glgBCDAP

Species

Bacillus subtilis/1423

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

glgB - CAA81040.1 463 - 2347 (+) Z25795.1:926-2810 2.4.1.18
glgC - CAA81041.1 2343 - 3486 (+) Z25795.1:2806-3949 2.7.7.27
glgD - CAA81042.1 3509 - 4541 (+) Z25795.1:3972-5004 -
glgA - CAA81043.1 4537 - 5992 (+) Z25795.1:5000-6455 2.4.1.21
glgP - CAA81044.1 5978 - 8375 (+) Z25795.1:6441-8838 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 464 - 2347 (+) CAZyme: GH13|CBM48|GH13_9 No
- 2344 - 3486 (+) CDS No
- 3510 - 4541 (+) CDS No
- 4538 - 5992 (+) CAZyme: GT5 No
- 5979 - 8375 (+) CAZyme: GT35 No

PUL ID

PUL0058

PubMed

8145641, Mol Microbiol. 1994 Jan;11(1):203-18. doi: 10.1111/j.1365-2958.1994.tb00301.x.

Title

Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation.

Author

Kiel JA, Boels JM, Beldman G, Venema G

Abstract

Although it has never been reported that Bacillus subtilis is capable of accumulating glycogen, we have isolated a region from the chromosome of B. subtilis containing a glycogen operon. The operon is located directly downstream from trnB, which maps at 275 degrees on the B. subtilis chromosome. It encodes five polypeptides with extensive similarity to enzymes involved in glycogen and starch metabolism in both prokaryotes and eukaryotes. The operon is presumably expressed by an E sigma E-controlled promoter, which was previously identified downstream from trnB. We have observed glycogen biosynthesis in B. subtilis exclusively on media containing carbon sources that allow efficient sporulation. Sporulation-independent synthesis of glycogen occurred after integration of an E sigma A controlled promoter upstream of the operon.