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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0062

PubMed

31421354, Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8.

Characterization method

NMR,sequence homology analysis

Genomic accession number

NZ_KB849308.1

Nucelotide position range

571525-595000

Substrate

capsule polysaccharide

Loci

wzc- wzb- wza- gna+ wzx+ gtr75+ gtr76+ wzy+ gtr77+ gtr9+ itrA2+ gaIU+ ugd+ gpi+ gen1+ pgt1+ pgm-

Species

Acinetobacter baumannii/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- F979_RS06965 WP_000030410.1 0 - 723 (-) NZ_KB849308.1:571525-572248 -
- F979_RS06970 WP_004735643.1 916 - 3112 (-) NZ_KB849308.1:572441-574637 2.7.10.2
- F979_RS06975 WP_002050525.1 3133 - 3562 (-) NZ_KB849308.1:574658-575087 -
- F979_RS06980 WP_025469400.1 3563 - 4664 (-) NZ_KB849308.1:575088-576189 -
tviB F979_RS06985 WP_004735649.1 4868 - 6146 (+) NZ_KB849308.1:576393-577671 -
- F979_RS06990 WP_002123321.1 6148 - 7438 (+) NZ_KB849308.1:577673-578963 -
- F979_RS06995 WP_004735653.1 7437 - 8385 (+) NZ_KB849308.1:578962-579910 -
- F979_RS07000 WP_002123295.1 8534 - 9542 (+) NZ_KB849308.1:580059-581067 -
- F979_RS07005 WP_002123290.1 9548 - 10589 (+) NZ_KB849308.1:581073-582114 -
- F979_RS07010 WP_004735655.1 10602 - 11637 (+) NZ_KB849308.1:582127-583162 -
- F979_RS07015 WP_002123301.1 11643 - 12471 (+) NZ_KB849308.1:583168-583996 -
- F979_RS07020 WP_004735659.1 12483 - 13104 (+) NZ_KB849308.1:584008-584629 -
galU F979_RS07025 WP_004735661.1 13128 - 14004 (+) NZ_KB849308.1:584653-585529 2.7.7.9
- F979_RS07030 WP_000686130.1 14119 - 15382 (+) NZ_KB849308.1:585644-586907 -
- F979_RS07035 WP_004735663.1 15378 - 17049 (+) NZ_KB849308.1:586903-588574 5.3.1.9
galE F979_RS07040 WP_004735665.1 17041 - 18061 (+) NZ_KB849308.1:588566-589586 5.1.3.2
- F979_RS07045 WP_114889769.1 18376 - 20038 (+) NZ_KB849308.1:589901-591563 -
- F979_RS07050 WP_000209962.1 20064 - 21435 (-) NZ_KB849308.1:591589-592960 -
lldP F979_RS07055 WP_170248999.1 21814 - 23476 (+) NZ_KB849308.1:593339-595001 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 723 (-) CDS No
- 917 - 3112 (-) TC: gnl|TC-DB|Q45409|8.A.3.3.3 Yes
- 3134 - 3562 (-) other Yes
- 3564 - 4664 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
tviB 4869 - 6146 (+) other Yes
- 6149 - 7438 (+) other Yes
- 7438 - 8385 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 8535 - 9542 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 9549 - 10589 (+) other Yes
- 10603 - 11637 (+) CAZyme: GT4 Yes
- 11644 - 12471 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 12484 - 13104 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
galU 13129 - 14004 (+) other Yes
- 14120 - 15382 (+) other Yes
- 15379 - 17049 (+) other Yes
galE 17042 - 18061 (+) other Yes
- 18377 - 20038 (+) other Yes
- 20065 - 21435 (-) other Yes
lldP 21815 - 23476 (+) TC: gnl|TC-DB|P33231|2.A.14.1.1 Yes

PUL ID

PUL0062

PubMed

31421354, Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8.

Title

Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures.

Author

Shashkov AS, Cahill SM, Arbatsky NP, Westacott AC, Kasimova AA, Shneider MM, Popova AV, Shagin DA, Shelenkov AA, Mikhailova YV, Yanushevich YG, Edelstein MV, Kenyon JJ, Knirel YA

Abstract

The genome of Acinetobacter baumannii clinical isolate, MAR-303, recovered in Russia was sequenced and found to contain a novel gene cluster at the A. baumannii K locus for capsule biosynthesis. The gene cluster, designated KL116, included four genes for glycosyltransferases (Gtrs) and a gene for a Wzy polymerase responsible for joining oligosaccharide K units into the capsular polysaccharide (CPS). The arrangement of KL116 was a hybrid of previously described A. baumannii gene clusters, with two gtr genes and the wzy gene shared by KL37 and the two other gtr genes found in KL14. The structure of the K116 CPS was established by sugar analysis and Smith degradation, along with one- and two-dimensional (1)H and (13)C NMR spectroscopy. The CPS is composed of branched pentasaccharide K units containing only neutral sugars, with three monosaccharides in the main chain and a disaccharide side chain. The K116 unit shares internal sugar linkages with the K14 and K37 units, corresponding to the presence of shared gtr genes in the gene clusters. However, the specific linkage formed by Wzy was discrepant between K116 and the previously reported K37 CPS produced by A. baumannii isolate NIPH146. The K37 structure was therefore revised in this study, and the corrected Wzy linkage found to be identical to the Wzy linkage in K116. The KL116, KL14 and KL37 gene clusters were found in genomes of a variety of A. baumannii strain backgrounds, indicating their global distribution.