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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0117

PubMed

31160824, Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.

Characterization method

recombinant protein expression,RNA-Seq,differential gene expression

Genomic accession number

AE015928.1

Nucelotide position range

1300094-1337010

Substrate

N-glycan

Loci

BT_1032-BT_1053

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_1032 AAO76139.1 0 - 2271 (-) AE015928.1:1300094-1302365 -
- BT_1033 AAO76140.1 2278 - 3247 (-) AE015928.1:1302372-1303341 -
- BT_1034 AAO76141.1 3299 - 4631 (-) AE015928.1:1303393-1304725 -
- BT_1035 AAO76142.1 4729 - 6934 (-) AE015928.1:1304823-1307028 -
- BT_1036 AAO76143.1 7100 - 8450 (-) AE015928.1:1307194-1308544 -
- BT_1037 AAO76144.1 8466 - 9654 (-) AE015928.1:1308560-1309748 -
- BT_1038 AAO76145.1 9697 - 10720 (-) AE015928.1:1309791-1310814 -
- BT_1039 AAO76146.1 10746 - 12330 (-) AE015928.1:1310840-1312424 -
- BT_1040 AAO76147.1 12338 - 15101 (-) AE015928.1:1312432-1315195 -
- BT_1041 AAO76148.1 15901 - 17143 (-) AE015928.1:1315995-1317237 -
- BT_1042 AAO76149.1 17511 - 20805 (+) AE015928.1:1317605-1320899 -
- BT_1043 AAO76150.1 20825 - 22466 (+) AE015928.1:1320919-1322560 -
- BT_1044 AAO76151.1 22497 - 23592 (+) AE015928.1:1322591-1323686 -
- BT_1045 AAO76152.1 23601 - 24792 (+) AE015928.1:1323695-1324886 -
- BT_1046 AAO76153.1 24981 - 27804 (+) AE015928.1:1325075-1327898 -
- BT_1047 AAO76154.1 27822 - 29367 (+) AE015928.1:1327916-1329461 -
- BT_1048 AAO76155.1 29391 - 30519 (+) AE015928.1:1329485-1330613 -
- BT_1049 AAO76156.1 30525 - 31707 (+) AE015928.1:1330619-1331801 -
- BT_1050 AAO76157.1 31735 - 32641 (+) AE015928.1:1331829-1332735 -
- BT_1051 AAO76158.1 32771 - 35093 (+) AE015928.1:1332865-1335187 -
- BT_1052 AAO76159.1 35152 - 36145 (-) AE015928.1:1335246-1336239 -
- BT_1053 AAO76160.1 36308 - 36917 (+) AE015928.1:1336402-1337011 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2271 (-) CAZyme: GH92 Yes
- 2279 - 3247 (-) CAZyme: GH130 Yes
- 3300 - 4631 (-) other Yes
- 4730 - 6934 (-) CAZyme: GH163 Yes
- 7101 - 8450 (-) other Yes
- 8467 - 9654 (-) other Yes
- 9698 - 10720 (-) other Yes
- 10747 - 12330 (-) TC: gnl|TC-DB|Q8A8X0|8.A.46.2.1 Yes
- 12339 - 15101 (-) TC: gnl|TC-DB|Q8A8X1|1.B.14.6.13 Yes
- 15902 - 17143 (-) other Yes
- 17512 - 20805 (+) TC: gnl|TC-DB|Q8A8X1|1.B.14.6.13 Yes
- 20826 - 22466 (+) TC: gnl|TC-DB|Q8A0N7|8.A.46.2.2 Yes
- 22498 - 23592 (+) other Yes
- 23602 - 24792 (+) other Yes
- 24982 - 27804 (+) TC: gnl|TC-DB|Q8A8X1|1.B.14.6.13 Yes
- 27823 - 29367 (+) TC: gnl|TC-DB|Q8A8X0|8.A.46.2.1 Yes
- 29392 - 30519 (+) other Yes
- 30526 - 31707 (+) other Yes
- 31736 - 32641 (+) other Yes
- 32772 - 35093 (+) CAZyme: GH20 Yes
- 35153 - 36145 (-) STP: STP|FecR No
- 36309 - 36917 (+) TF: DBD-Pfam|GerE No

PUL ID

PUL0117

PubMed

31160824, Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.

Title

Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.

Author

Briliute J, Urbanowicz PA, Luis AS, Basle A, Paterson N, Rebello O, Hendel J, Ndeh DA, Lowe EC, Martens EC, Spencer DIR, Bolam DN, Crouch LI

Abstract

Glycans are the major carbon sources available to the human colonic microbiota. Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels. Here, we show that many mutualistic gut Bacteroides spp. have the capacity to utilize complex N-glycans (CNGs) as nutrients, including those from immunoglobulins. Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation. The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity. Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan. This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.