Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0179

PubMed

27288925, FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.

Characterization method

enzyme activity assay,enzyme specificity assay,substrate specificity assay

Genomic accession number

JH724241.1

Nucelotide position range

777587-798381

Substrate

galactomannan

Loci

HMPREF1070_00547-HMPREF1070_00560

Species

Bacteroides ovatus/28116

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HMPREF1070_00547 EIY71129.1 0 - 840 (-) JH724241.1:777587-778427 -
- HMPREF1070_00548 EIY71130.1 867 - 2058 (-) JH724241.1:778454-779645 -
- HMPREF1070_00549 EIY71131.1 2071 - 3355 (-) JH724241.1:779658-780942 -
- HMPREF1070_00550 EIY71132.1 3362 - 4730 (-) JH724241.1:780949-782317 -
- HMPREF1070_00551 EIY71133.1 4726 - 5905 (-) JH724241.1:782313-783492 -
- HMPREF1070_00552 EIY71134.1 5923 - 6994 (-) JH724241.1:783510-784581 -
- HMPREF1070_00553 EIY71135.1 7029 - 8229 (-) JH724241.1:784616-785816 -
- HMPREF1070_00554 EIY71136.1 8468 - 9260 (-) JH724241.1:786055-786847 -
- HMPREF1070_00555 EIY71137.1 9325 - 10498 (-) JH724241.1:786912-788085 -
- HMPREF1070_00556 EIY71138.1 10531 - 12328 (-) JH724241.1:788118-789915 -
- HMPREF1070_00557 EIY71139.1 12351 - 15597 (-) JH724241.1:789938-793184 -
- HMPREF1070_00558 EIY71140.1 15652 - 16093 (-) JH724241.1:793239-793680 -
- HMPREF1070_00559 EIY71141.1 16125 - 16566 (-) JH724241.1:793712-794153 -
- HMPREF1070_00560 EIY71142.1 16802 - 20795 (+) JH724241.1:794389-798382 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 840 (-) CDS No
- 868 - 2058 (-) CDS No
- 2072 - 3355 (-) CAZyme: CE7 Yes
- 3363 - 4730 (-) TC: gnl|TC-DB|A1S5F2|2.A.2.3.6 Yes
- 4727 - 5905 (-) CAZyme: GH130 Yes
- 5924 - 6994 (-) CAZyme: GH26 Yes
- 7030 - 8229 (-) CAZyme: GH26 Yes
- 8469 - 9260 (-) other Yes
- 9326 - 10498 (-) other Yes
- 10532 - 12328 (-) other Yes
- 12352 - 15597 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 15653 - 16093 (-) CDS No
- 16126 - 16566 (-) CDS No
- 16803 - 20795 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 No
- 20792 - 20795 (-) CDS No

PUL ID

PUL0179

PubMed

27288925, FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.

Title

A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans.

Author

Reddy SK, Bagenholm V, Pudlo NA, Bouraoui H, Koropatkin NM, Martens EC, Stalbrand H

Abstract

The Bacova_02091 gene in the beta-mannan utilization locus of Bacteroides ovatus encodes a family GH36 alpha-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterization of recombinant BoGal36A reveals unique properties compared to other GH36 alpha-galactosidases, which preferentially hydrolyse terminal alpha-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (> 90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone.