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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0182

PubMed

27058293, Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.

Characterization method

NMR,sugar utilization assay

Genomic accession number

AB811608

Nucelotide position range

434-13616

Substrate

O-antigen

Loci

galF-orf1-wegS-wzx-orf4-wzy-orf6-gnd

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ00752.1 434 - 1826 (+) AB811608.1:868-2260 -
galF - BAQ00753.1 1960 - 2869 (+) AB811608.1:2394-3303 -
- - BAQ00754.1 3045 - 3873 (+) AB811608.1:3479-4307 -
- - BAQ00755.1 3895 - 4288 (+) AB811608.1:4329-4722 -
wzx - BAQ00756.1 4306 - 5566 (+) AB811608.1:4740-6000 -
- - BAQ00757.1 5547 - 6471 (+) AB811608.1:5981-6905 -
wzy - BAQ00758.1 6467 - 7688 (+) AB811608.1:6901-8122 -
- - BAQ00759.1 7680 - 8859 (+) AB811608.1:8114-9293 -
gnd - BAQ00760.1 9007 - 10414 (+) AB811608.1:9441-10848 -
ugd - BAQ00761.1 10646 - 11813 (+) AB811608.1:11080-12247 -
wzz - BAQ00762.1 11958 - 12957 (+) AB811608.1:12392-13391 -
hisI - BAQ00763.1 13016 - 13616 (-) AB811608.1:13450-14050 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 435 - 1826 (+) CDS No
- 1961 - 2869 (+) CDS No
- 3046 - 3873 (+) CDS No
- 3896 - 4288 (+) CDS No
- 4307 - 5566 (+) CAZyme: GT2|GT2_Glycos_transf_2 No
- 5548 - 6471 (+) CDS No
- 6468 - 7688 (+) CDS No
- 7681 - 8859 (+) CDS No
- 9008 - 10414 (+) CDS No
- 10647 - 11813 (+) CDS No
- 11959 - 12957 (+) CDS No
- 13017 - 13616 (-) CDS No

PUL ID

PUL0182

PubMed

27058293, Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.

Title

Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100.

Author

Shashkov AS, Yang B, Senchenkova SN, Perepelov AV, Liu B, Knirel YA

Abstract

O-polysaccharides of E. coli O28ab, O37, and O100 were found to contain glycerol 1-phosphate and the following structures of their oligosaccharide repeats were established by sugar analysis, Smith degradation (for O28ab), 1D and 2D (1)H, (13)C, and (13)P NMR spectroscopy: [Formula: see text]. Functions of putative glycosyltransferases genes in the O-antigen gene clusters of the strains studied were tentatively assigned based on similarities to genes of other E. coli O-serogroups available from GenBank and taking into account the O-polysaccharide structures established.