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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0183

PubMed

27058293, Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.

Characterization method

NMR,sugar utilization assay

Genomic accession number

AB811614

Nucelotide position range

1-19104

Substrate

O-antigen

Loci

BAQ00839.1-BAQ00853.1

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ00839.1 0 - 901 (+) AB811614.1:1-902 -
gne - BAQ00840.1 1058 - 2054 (+) AB811614.1:1059-2055 -
galF - BAQ00841.1 2296 - 3190 (+) AB811614.1:2297-3191 -
- - BAQ00842.1 6721 - 7777 (+) AB811614.1:6722-7778 -
- - BAQ00843.1 8300 - 8693 (+) AB811614.1:8301-8694 -
wzx - BAQ00844.1 8694 - 9927 (+) AB811614.1:8695-9928 -
- - BAQ00845.1 9926 - 10655 (+) AB811614.1:9927-10656 -
wzy - BAQ00846.1 10657 - 11791 (+) AB811614.1:10658-11792 -
- - BAQ00847.1 11777 - 12668 (+) AB811614.1:11778-12669 -
- - BAQ00848.1 12664 - 13732 (+) AB811614.1:12665-13733 -
- - BAQ00849.1 13703 - 14540 (+) AB811614.1:13704-14541 -
gnd - BAQ00850.1 14654 - 16061 (+) AB811614.1:14655-16062 -
ugd - BAQ00851.1 16309 - 17476 (+) AB811614.1:16310-17477 -
wzz - BAQ00852.1 17565 - 18600 (+) AB811614.1:17566-18601 -
hisI - BAQ00853.1 18771 - 19104 (-) AB811614.1:18772-19105 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 901 (+) CDS No
- 1059 - 2054 (+) CDS No
- 2297 - 3190 (+) CDS No
- 6722 - 7777 (+) CDS No
- 8301 - 8693 (+) CDS No
- 8695 - 9927 (+) CDS No
- 9927 - 10655 (+) CDS No
- 10658 - 11791 (+) CAZyme: GT2_Glycos_transf_2|GT2 No
- 11778 - 12668 (+) CDS No
- 12665 - 13732 (+) CDS No
- 13704 - 14540 (+) CDS No
- 14655 - 16061 (+) CDS No
- 16310 - 17476 (+) CDS No
- 17566 - 18600 (+) CDS No
- 18772 - 19104 (-) CDS No

PUL ID

PUL0183

PubMed

27058293, Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.

Title

Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100.

Author

Shashkov AS, Yang B, Senchenkova SN, Perepelov AV, Liu B, Knirel YA

Abstract

O-polysaccharides of E. coli O28ab, O37, and O100 were found to contain glycerol 1-phosphate and the following structures of their oligosaccharide repeats were established by sugar analysis, Smith degradation (for O28ab), 1D and 2D (1)H, (13)C, and (13)P NMR spectroscopy: [Formula: see text]. Functions of putative glycosyltransferases genes in the O-antigen gene clusters of the strains studied were tentatively assigned based on similarities to genes of other E. coli O-serogroups available from GenBank and taking into account the O-polysaccharide structures established.