dbCAN-PUL

PUL ID	PUL0213
PubMed	25483279, Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.
Characterization method	qRT-PCR
Genomic accession number	NC_008618.1
Nucelotide position range	2044437-2051250
Substrate	galactooligosaccharide
Loci	BAD_RS08450-BAD_RS08465
Species	Bifidobacterium adolescentis/1680
Degradation or Biosynthesis	degradation

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 1527 (-)	TC: gnl\|TC-DB\|Q9X761\|2.A.2.2.3	Yes
-	1853 - 4924 (+)	CAZyme: GH2	Yes
-	5219 - 6238 (-)	TF: DBD-Pfam\|LacI,DBD-SUPERFAMILY\|0044558	No
-	6320 - 6814 (+)	TF: DBD-Pfam\|HTH_AraC,DBD-Pfam\|HTH_AraC,DBD-Pfam\|HTH_AraC,DBD-SUPERFAMILY\|0036286,DBD-SUPERFAMILY\|0035607	No

PUL ID	PUL0213
PubMed	25483279, Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.
Title	Diverse galactooligosaccharides consumption by bifidobacteria: implications of beta-galactosidase--LacS operon.
Author	Akiyama T, Kimura K, Hatano H
Abstract	Galactooligosaccharides (GOS) possess prebiotic properties that specifically increase the number of bifidobacteria in the human intestine, thus giving health benefits to the host. Although the bifidogenic effect of GOS has been demonstrated in numerous studies, the utilization of GOS by specific bifidobacteria remains unclear. The goal of our study was to elucidate GOS consumption by specific bifidobacteria and gain insights into the mechanism. First, we examined GOS consumption by 14 bifidobacterial strains belonging to seven different species by comparing growth rate, carbohydrate consumption, and acid production. We then performed a transcription analysis in the case of one strong GOS consumer, Bifidobacterium adolescentis YIT 4011(T), to predict the operon contributing to GOS use. The study indicated the contribution of an operon consisted of LacS symporter and beta-galactosidase to bifidobacterial GOS consumption.