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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0219

PubMed

17644636, Appl Environ Microbiol. 2007 Sep;73(18):5716-24. doi: 10.1128/AEM.00805-07. Epub 2007 Jul 20.

Characterization method

sugar utilization assay,enzyme activity assay

Genomic accession number

DQ396803.1

Nucelotide position range

1-10449

Substrate

fructooligosaccharide,fructan

Loci

fosABCDXE

Species

Lactobacillus paracasei/1597

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

fosR - ABD57313.1 0 - 2535 (-) DQ396803.1:1-2536 -
fosA - ABD57314.1 2818 - 3256 (+) DQ396803.1:2819-3257 -
fosB - ABD57315.1 3268 - 3763 (+) DQ396803.1:3269-3764 -
fosC - ABD57316.1 4035 - 4899 (+) DQ396803.1:4036-4900 -
fosD - ABD57317.1 4901 - 5747 (+) DQ396803.1:4902-5748 -
fosX - ABD57318.1 5780 - 6113 (+) DQ396803.1:5781-6114 -
fosE - ABD57319.1 6318 - 10449 (+) DQ396803.1:6319-10450 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2535 (-) CDS No
- 2819 - 3256 (+) CDS No
- 3269 - 3763 (+) CDS No
- 4036 - 4899 (+) CDS No
- 4902 - 5747 (+) TC: gnl|TC-DB|D0ZLR4|4.A.6.1.17 Yes
- 5781 - 6113 (+) other Yes
- 6319 - 10449 (+) CAZyme: GH32 Yes

PUL ID

PUL0219

PubMed

17644636, Appl Environ Microbiol. 2007 Sep;73(18):5716-24. doi: 10.1128/AEM.00805-07. Epub 2007 Jul 20.

Title

Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195.

Author

Goh YJ, Lee JH, Hutkins RW

Abstract

The fosABCDXE operon encodes components of a putative fructose/mannose phosphoenolpyruvate-dependent phosphotransferase system and a beta-fructosidase precursor (FosE) that are involved in the fructooligosaccharide (FOS) utilization pathway of Lactobacillus paracasei 1195. The presence of an N-terminal signal peptide sequence and an LPQAG cell wall anchor motif in the C-terminal region of the deduced FosE precursor amino acid sequence predicted that the enzyme is cell wall associated, indicating that FOS may be hydrolyzed extracellularly. In this study, cell fractionation experiments demonstrated that the FOS hydrolysis activity was present exclusively in the cell wall extract of L. paracasei previously grown on FOS. In contrast, no measurable FOS hydrolysis activity was detected in the cell wall extract from the isogenic fosE mutant. Induction of beta-fructosidase activity was observed when cells were grown on FOS, inulin, sucrose, or fructose but not when cells were grown on glucose. A diauxic growth pattern was observed when cells were grown on FOS in the presence of limiting glucose (0.1%). Analysis of the culture supernatant revealed that glucose was consumed first, followed by the longer-chain FOS species. Transcription analysis further showed that the fos operon was expressed only after glucose was depleted in the medium. Expression of fosE in a non-FOS-fermenting strain, Lactobacillus rhamnosus GG, enabled the recombinant strain to metabolize FOS, inulin, sucrose, and levan.