dbCAN-PUL

PUL ID	PUL0238
PubMed	18177310, FEMS Microbiol Lett. 2008 Feb;279(1):103-9. doi: 10.1111/j.1574-6968.2007.01018.x.
Characterization method	Northern Blot
Genomic accession number	NC_000964.3
Nucelotide position range	626622-633862
Substrate	glucomannan
Loci	gmuBACDREFG
Species	Bacillus subtilis/1423
Degradation or Biosynthesis	degradation

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
gmuB	1 - 312 (+)	TC: gnl\|TC-DB\|Q9KSH5\|4.A.3.2.6	Yes
gmuA	312 - 644 (+)	TC: gnl\|TC-DB\|Q72XQ1\|4.A.3.2.8	Yes
gmuC	663 - 1991 (+)	TC: gnl\|TC-DB\|Q72XQ0\|4.A.3.2.8	Yes
gmuD	2009 - 3406 (+)	TC: gnl\|TC-DB\|Q86Z14\|8.A.49.1.2	Yes
gmuR	3549 - 4262 (+)	TF: DBD-Pfam\|GntR,DBD-SUPERFAMILY\|0037767	Yes
gmuE	4291 - 5190 (+)	other	Yes
gmuF	5187 - 6134 (+)	other	Yes
gmuG	6153 - 7241 (+)	CAZyme: GH26	Yes

PUL ID	PUL0238
PubMed	18177310, FEMS Microbiol Lett. 2008 Feb;279(1):103-9. doi: 10.1111/j.1574-6968.2007.01018.x.
Title	Glucomannan utilization operon of Bacillus subtilis.
Author	Sadaie Y, Nakadate H, Fukui R, Yee LM, Asai K
Abstract	This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong inducers of transcription. It is shown that an internal regulator gene (gmuR) encodes a repressor of the operon, as disruption of this gene enhances transcription of the operon in the absence of inducers. The expression of the glucomannan utilizing operon of B. subtilis is thus induced by degraded glucomannan products, and repressed by an internal repressor.