PUL ID

PUL0242

PubMed

24448980, J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.

Characterization method

fosmid library screen,sequence homology analysis

Genomic accession number

JX424627

Nucelotide position range

4542-17611

Substrate

cellulose

Loci

AGH14097.1-AGH14104.1

Species

Prevotella sp. Sc00044/1231730

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - AGH14097.1 0 - 2166 (-) JX424627.1:4542-6708 -
- - AGH14098.1 2406 - 4065 (+) JX424627.1:6948-8607 -
- - AGH14099.1 4099 - 5377 (-) JX424627.1:8641-9919 -
- - AGH14100.1 5373 - 5769 (-) JX424627.1:9915-10311 -
- - AGH14101.1 5854 - 7687 (-) JX424627.1:10396-12229 -
- - AGH14102.1 7703 - 9083 (-) JX424627.1:12245-13625 -
- - AGH14103.1 9102 - 10845 (-) JX424627.1:13644-15387 -
- - AGH14104.1 10883 - 13070 (-) JX424627.1:15425-17612 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 4 (+) CDS No
- 1 - 2166 (-) CAZyme: GH31 Yes
- 2407 - 4065 (+) CAZyme: CBM72|GH5_4 Yes
- 4100 - 5377 (-) CAZyme: GH26 Yes
- 5374 - 5769 (-) other Yes
- 5855 - 7687 (-) other Yes
- 7704 - 9083 (-) other Yes
- 9103 - 10845 (-) other Yes
- 10884 - 13070 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes

PUL ID

PUL0242

PubMed

24448980, J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.

Title

Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes.

Author

Rosewarne CP, Pope PB, Cheung JL, Morrison M

Abstract

Several unique Sus-like polysaccharide utilization loci (PULs) were identified from bacteria resident in bovine rumen microbiomes through functional screening of a fosmid library. The loci were phylogenetically assigned to the genus Prevotella within the phylum Bacteroidetes. These findings were augmented by a bioinformatic re-evaluation of ruminal Prevotella genomes, revealing additional loci not previously reported in the literature. Analysis of Bacteroidales-affiliated genomes reconstructed from a bovine rumen metagenome in a previous study further expanded the diversity of Sus-like PULs resident in this microbiome. Our findings suggest that Sus-like systems represent an important mechanism for degradation of a range of plant-derived glycans in ruminants.