dbCAN-PUL

PUL ID	PUL0243
PubMed	24448980, J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
Characterization method	fosmid library screen,sequence homology analysis
Genomic accession number	JX424628
Nucelotide position range	13465-32307
Substrate	cellulose
Loci	AGH14126.1-AGH14137.1
Species	Prevotella sp. Sc00066/1231731
Degradation or Biosynthesis	degradation

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 2691 (+)	TF: DBD-Pfam\|HTH_AraC,DBD-Pfam\|HTH_AraC,DBD-SUPERFAMILY\|0036286,DBD-SUPERFAMILY\|0035607	No
-	2879 - 4060 (+)	CAZyme: GH5_38	Yes
-	4139 - 4906 (+)	other	Yes
-	4903 - 5262 (+)	other	Yes
-	5366 - 7906 (+)	CAZyme: GH94	Yes
-	7911 - 9251 (+)	TC: gnl\|TC-DB\|P31435\|2.A.2.3.4	Yes
-	9286 - 12459 (+)	TC: gnl\|TC-DB\|Q93TH9\|1.B.14.6.2	Yes
-	12474 - 14225 (+)	TC: gnl\|TC-DB\|Q8A0N7\|8.A.46.2.2	Yes
-	14266 - 15588 (+)	other	Yes
-	15751 - 16254 (+)	other	Yes
-	16279 - 16518 (+)	other	Yes
-	16678 - 18843 (+)	CAZyme: GH36	Yes

PUL ID	PUL0243
PubMed	24448980, J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
Title	Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes.
Author	Rosewarne CP, Pope PB, Cheung JL, Morrison M
Abstract	Several unique Sus-like polysaccharide utilization loci (PULs) were identified from bacteria resident in bovine rumen microbiomes through functional screening of a fosmid library. The loci were phylogenetically assigned to the genus Prevotella within the phylum Bacteroidetes. These findings were augmented by a bioinformatic re-evaluation of ruminal Prevotella genomes, revealing additional loci not previously reported in the literature. Analysis of Bacteroidales-affiliated genomes reconstructed from a bovine rumen metagenome in a previous study further expanded the diversity of Sus-like PULs resident in this microbiome. Our findings suggest that Sus-like systems represent an important mechanism for degradation of a range of plant-derived glycans in ruminants.