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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0246

PubMed

24333485, J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.

Characterization method

enzyme activity assay,gene deletion mutant and growth assay,Western Blot

Genomic accession number

AAZZ01000003.1

Nucelotide position range

96120-112614

Substrate

fucose

Loci

CGSSp3BS71_10393-CGSSp3BS71_10443

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- CGSSp3BS71_10393 EDK74347.1 0 - 1767 (-) AAZZ01000003.1:96120-97887 -
- CGSSp3BS71_10398 EDK74348.1 1779 - 2508 (-) AAZZ01000003.1:97899-98628 -
- CGSSp3BS71_10403 EDK74349.1 2522 - 5540 (-) AAZZ01000003.1:98642-101660 -
- CGSSp3BS71_10408 EDK74350.1 5550 - 7767 (-) AAZZ01000003.1:101670-103887 -
- CGSSp3BS71_10413 EDK74351.1 8006 - 9659 (-) AAZZ01000003.1:104126-105779 -
- CGSSp3BS71_10418 EDK74352.1 9664 - 10972 (-) AAZZ01000003.1:105784-107092 -
- CGSSp3BS71_10423 EDK74353.1 10981 - 11827 (-) AAZZ01000003.1:107101-107947 -
- CGSSp3BS71_10428 EDK74354.1 11823 - 12753 (-) AAZZ01000003.1:107943-108873 -
- CGSSp3BS71_10433 EDK74355.1 12839 - 14132 (-) AAZZ01000003.1:108959-110252 -
- CGSSp3BS71_10438 EDK74356.1 14160 - 15564 (-) AAZZ01000003.1:110280-111684 -
- CGSSp3BS71_10443 EDK74357.1 15739 - 16495 (+) AAZZ01000003.1:111859-112615 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1767 (-) CDS No
- 1780 - 2508 (-) CDS No
- 2523 - 5540 (-) CAZyme: GH98|CBM51 Yes
- 5551 - 7767 (-) CAZyme: GH36 Yes
- 8007 - 9659 (-) CAZyme: GH36 Yes
- 9665 - 10972 (-) CAZyme: GH29 Yes
- 10982 - 11827 (-) TC: gnl|TC-DB|A5LBQ4|3.A.1.1.47 Yes
- 11824 - 12753 (-) TC: gnl|TC-DB|A5LBQ5|3.A.1.1.47 Yes
- 12840 - 14132 (-) TC: gnl|TC-DB|A5LBQ6|3.A.1.1.47 Yes
- 14161 - 15564 (-) CDS No
- 15740 - 16495 (+) TF: DBD-Pfam|HTH_DeoR,DBD-SUPERFAMILY|0043758 No

PUL ID

PUL0246

PubMed

24333485, J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.

Title

Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae.

Author

Higgins MA, Suits MD, Marsters C, Boraston AB

Abstract

Fucose metabolism pathways are present in many bacterial species and typically contain the central fucose-processing enzymes fucose isomerase (FcsI), fuculose kinase (FcsK), and fuculose-1-phosphate aldolase (FcsA). Fucose initially undergoes isomerization by FcsI producing fuculose, which is then phosphorylated by FcsK. FcsA cleaves the fuculose-1-phosphate product into lactaldehyde and dihydroxyacetone phosphate, which can be incorporated into central metabolism allowing the bacterium to use fucose as an energy source. Streptococcus pneumoniae has fucose-processing operons containing homologs of FcsI, FcsK, and FcsA; however, this bacterium appears unable to utilize fucose as an energy source. To investigate this contradiction, we performed biochemical and structural studies of the S. pneumoniae fucose-processing enzymes SpFcsI, SpFcsK, and SpFcsA. These enzymes are demonstrated to act in a sequential manner to ultimately produce dihydroxyacetone phosphate and have structural features entirely consistent with their observed biochemical activities. Analogous to the regulation of the Escherichia coli fucose utilization operon, fuculose-1-phosphate appears to act as an inducing molecule for activation of the S. pneumoniae fucose operon. Despite our evidence that S. pneumoniae appears to have the appropriate regulatory and biochemical machinery for fucose metabolism, we confirmed the inability of the S. pneumoniae TIGR4 strain to grow on fucose or on the H-disaccharide, which is the probable substrate of the transporter for the pathway. On the basis of these observations, we postulate that the S. pneumoniae fucose-processing pathway has a non-metabolic role in the interaction of this bacterium with its human host.