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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0272

PubMed

23663691, BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.

Characterization method

RT-qPCR

Genomic accession number

CP001515.1

Nucelotide position range

565133-571261

Substrate

beta-galactooligosaccharide

Loci

balac_0475-balac_0477

Species

Bifidobacterium animalis subsp. lactis/302911

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

lacS Balac_0475 ACS45854.1 0 - 1518 (-) CP001515.1:565133-566651 -
lacZ Balac_0476 ACS45855.1 1827 - 5031 (+) CP001515.1:566960-570164 -
- Balac_0477 ACS45856.1 5133 - 6129 (-) CP001515.1:570266-571262 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

lacS 1 - 1518 (-) TC: gnl|TC-DB|Q9X761|2.A.2.2.3 Yes
lacZ 1828 - 5031 (+) CAZyme: GH2 Yes
- 5134 - 6129 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0044558 No

PUL ID

PUL0272

PubMed

23663691, BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.

Title

Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04.

Author

Andersen JM, Barrangou R, Abou Hachem M, Lahtinen SJ, Goh YJ, Svensson B, Klaenhammer TR

Abstract

BACKGROUND: Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of alpha- and beta-linked hexoses, and beta-xylosides. RESULTS: The overall transcriptome was modulated dependent on the type of glycoside (galactosides, glucosides or xylosides) utilized. Carbohydrate transporters of the major facilitator superfamily (induced by gentiobiose and beta-galacto-oligosaccharides (GOS)) and ATP-binding cassette (ABC) transporters (upregulated by cellobiose, GOS, isomaltose, maltotriose, melibiose, panose, raffinose, stachyose, xylobiose and beta-xylo-oligosaccharides) were differentially upregulated, together with glycoside hydrolases from families 1, 2, 13, 36, 42, 43 and 77. Sequence analysis of the identified solute-binding proteins that determine the specificity of ABC transporters revealed similarities in the breadth and selectivity of prebiotic utilization by bifidobacteria. CONCLUSION: This study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. Results provide insights into the ability of this probiotic microbe to utilize indigestible carbohydrates in the human gastrointestinal tract.