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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0274

PubMed

23663691, BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.

Characterization method

RT-qPCR

Genomic accession number

CP001515.1

Nucelotide position range

604574-622423

Substrate

xylobiose,xylooligosaccharide

Loci

balac_0511-balac_0522

Species

Bifidobacterium animalis subsp. lactis/302911

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Balac_0511 ACS45886.1 0 - 1353 (+) CP001515.1:604574-605927 5.3.1.5
- Balac_0512 ACS45887.1 1757 - 3440 (+) CP001515.1:606331-608014 -
- Balac_0513 ACS45888.1 3452 - 4457 (-) CP001515.1:608026-609031 -
- Balac_0514 ACS45889.1 4787 - 6065 (+) CP001515.1:609361-610639 -
- Balac_0515 ACS45890.1 6173 - 7232 (+) CP001515.1:610747-611806 -
- Balac_0516 ACS45891.1 7233 - 8103 (+) CP001515.1:611807-612677 -
- Balac_0517 ACS45892.1 8180 - 9806 (+) CP001515.1:612754-614380 -
- Balac_0518 ACS45893.1 9911 - 11837 (+) CP001515.1:614485-616411 -
- Balac_0519 ACS45894.1 12073 - 13237 (+) CP001515.1:616647-617811 -
- Balac_0520 ACS45895.1 13249 - 14809 (-) CP001515.1:617823-619383 -
- Balac_0521 ACS45896.1 14898 - 16428 (-) CP001515.1:619472-621002 -
- Balac_0522 ACS45897.1 16614 - 17850 (+) CP001515.1:621188-622424 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1353 (+) CDS No
- 1758 - 3440 (+) CAZyme: GH43|GH43_12 Yes
- 3453 - 4457 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 Yes
- 4788 - 6065 (+) STP: STP|SBP_bac_1 Yes
- 6174 - 7232 (+) TC: gnl|TC-DB|O51924|3.A.1.1.7 Yes
- 7234 - 8103 (+) TC: gnl|TC-DB|Q8RJU8|3.A.1.1.18 Yes
- 8181 - 9806 (+) CAZyme: GH43_11|GH43 Yes
- 9912 - 11837 (+) other Yes
- 12074 - 13237 (+) other Yes
- 13250 - 14809 (-) CAZyme: GH43_10 Yes
- 14899 - 16428 (-) CDS No
- 16615 - 17850 (+) STP: STP|HTH_IclR No

PUL ID

PUL0274

PubMed

23663691, BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.

Title

Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04.

Author

Andersen JM, Barrangou R, Abou Hachem M, Lahtinen SJ, Goh YJ, Svensson B, Klaenhammer TR

Abstract

BACKGROUND: Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of alpha- and beta-linked hexoses, and beta-xylosides. RESULTS: The overall transcriptome was modulated dependent on the type of glycoside (galactosides, glucosides or xylosides) utilized. Carbohydrate transporters of the major facilitator superfamily (induced by gentiobiose and beta-galacto-oligosaccharides (GOS)) and ATP-binding cassette (ABC) transporters (upregulated by cellobiose, GOS, isomaltose, maltotriose, melibiose, panose, raffinose, stachyose, xylobiose and beta-xylo-oligosaccharides) were differentially upregulated, together with glycoside hydrolases from families 1, 2, 13, 36, 42, 43 and 77. Sequence analysis of the identified solute-binding proteins that determine the specificity of ABC transporters revealed similarities in the breadth and selectivity of prebiotic utilization by bifidobacteria. CONCLUSION: This study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. Results provide insights into the ability of this probiotic microbe to utilize indigestible carbohydrates in the human gastrointestinal tract.