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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0290

PubMed

29524727, Carbohydr Res. 2018 May 2;460:51-56. doi: 10.1016/j.carres.2018.02.014. Epub 2018 Feb 28.

Characterization method

NMR,mass spectrometry,sugar utilization assay

Genomic accession number

MWTO01000004.1

Nucelotide position range

48471-60952

Substrate

O-antigen

Loci

B4916_03530-B4916_03600

Species

Yersinia intermedia/631

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- B4916_03530 OWF92357.1 0 - 1101 (-) MWTO01000004.1:48471-49572 -
- B4916_03535 WP_014609409.1 1332 - 1910 (-) MWTO01000004.1:49803-50381 -
- B4916_03540 WP_076611473.1 1846 - 2349 (-) MWTO01000004.1:50317-50820 -
- B4916_03545 OWF92358.1 2466 - 3483 (-) MWTO01000004.1:50937-51954 -
- B4916_03550 OWF92359.1 3931 - 5065 (-) MWTO01000004.1:52402-53536 -
- B4916_03555 OWF92360.1 5068 - 6154 (-) MWTO01000004.1:53539-54625 -
- B4916_03560 OWF92361.1 6163 - 7153 (-) MWTO01000004.1:54634-55624 -
- B4916_03565 OWF92362.1 7301 - 8000 (-) MWTO01000004.1:55772-56471 -
- B4916_03570 OWF92363.1 7990 - 8194 (-) MWTO01000004.1:56461-56665 -
- B4916_03575 OWF92364.1 8667 - 8985 (-) MWTO01000004.1:57138-57456 -
- B4916_03580 OWF92365.1 9032 - 9194 (-) MWTO01000004.1:57503-57665 -
- B4916_03585 WP_005182329.1 9186 - 10408 (-) MWTO01000004.1:57657-58879 -
- B4916_03590 OWF92366.1 10440 - 11547 (-) MWTO01000004.1:58911-60018 -
- B4916_03595 OWF92367.1 11548 - 12085 (-) MWTO01000004.1:60019-60556 -
- B4916_03600 OWF92368.1 12077 - 12482 (-) MWTO01000004.1:60548-60953 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1101 (-) CDS No
- 2467 - 3483 (-) CDS No
- 3932 - 5065 (-) CAZyme: GT4 Yes
- 5069 - 6154 (-) CAZyme: GT4 Yes
- 6164 - 7153 (-) other Yes
- 7302 - 8000 (-) other Yes
- 7991 - 8194 (-) other Yes
- 8668 - 8985 (-) other Yes
- 9033 - 9194 (-) TC: gnl|TC-DB|P75905|4.D.1.1.3 Yes
- 10441 - 11547 (-) CDS No
- 11549 - 12085 (-) CDS No
- 12078 - 12482 (-) CDS No

PUL ID

PUL0290

PubMed

29524727, Carbohydr Res. 2018 May 2;460:51-56. doi: 10.1016/j.carres.2018.02.014. Epub 2018 Feb 28.

Title

Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17).

Author

Sizova OV, Shashkov AS, Kondakova AN, Knirel YA, Shaikhutdinova RZ, Ivanov SA, Kislichkina AA, Kadnikova LA, Bogun AG, Dentovskaya SV

Abstract

Lipopolysaccharide was isolated from bacteria Yersinia intermedia H9-36/83 (O:17) and degraded with mild acid to give an O-specific polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar analysis and 1D and 2D NMR spectroscopy. The polysaccharide was found to contain 3-deoxy-3-[(R)-3-hydroxybutanoylamino]-d-fucose (d-Fuc3NR3Hb) and the following structure of the heptasaccharide repeating unit was established: The structure established is consistent with the gene content of the O-antigen gene cluster. The O-polysaccharide structure and gene cluster of Y. intermedia are related to those of Hafnia alvei 1211 and Escherichia coli O:103.