Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0375

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Characterization method

microarray,qPCR

Genomic accession number

AE015928.1

Nucelotide position range

3254829-3288563

Substrate

alpha-mannan

Loci

BT_2615-BT_2633

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_2615 AAO77722.1 0 - 1293 (-) AE015928.1:3254829-3256122 -
- BT_2616 AAO77723.1 1616 - 1892 (+) AE015928.1:3256445-3256721 -
- BT_2617 AAO77724.1 1867 - 3274 (-) AE015928.1:3256696-3258103 -
- BT_2618 AAO77725.1 3740 - 5021 (-) AE015928.1:3258569-3259850 -
- BT_2619 AAO77726.1 5025 - 7398 (-) AE015928.1:3259854-3262227 -
- BT_2620 AAO77727.1 8079 - 10029 (-) AE015928.1:3262908-3264858 -
- BT_2621 AAO77728.1 10054 - 11365 (-) AE015928.1:3264883-3266194 -
- BT_2622 AAO77729.1 11373 - 13542 (-) AE015928.1:3266202-3268371 -
- BT_2623 AAO77730.1 13638 - 15192 (-) AE015928.1:3268467-3270021 -
- BT_2624 AAO77731.1 15207 - 16173 (-) AE015928.1:3270036-3271002 -
- BT_2625 AAO77732.1 16184 - 18167 (-) AE015928.1:3271013-3272996 -
- BT_2626 AAO77733.1 18177 - 21282 (-) AE015928.1:3273006-3276111 -
- BT_2627 AAO77734.1 21315 - 22620 (-) AE015928.1:3276144-3277449 -
- BT_2628 AAO77735.1 23110 - 27148 (-) AE015928.1:3277939-3281977 -
- BT_2629 AAO77736.1 27358 - 29641 (+) AE015928.1:3282187-3284470 -
- BT_2630 AAO77737.1 29694 - 30645 (+) AE015928.1:3284523-3285474 -
- BT_2631 AAO77738.1 30697 - 31855 (+) AE015928.1:3285526-3286684 -
- BT_2632 AAO77739.1 31914 - 33360 (+) AE015928.1:3286743-3288189 -
- BT_2633 AAO77740.1 33468 - 33735 (+) AE015928.1:3288297-3288564 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1293 (-) CDS No
- 1617 - 1892 (+) CDS No
- 1868 - 3274 (-) CDS No
- 3741 - 5021 (-) TF: DBD-Pfam|HTH_8,DBD-SUPERFAMILY|0037176 No
- 5026 - 7398 (-) STP: STP|HisKA,STP|HATPase_c,STP|Response_reg No
- 8080 - 10029 (-) CAZyme: GH97 Yes
- 10055 - 11365 (-) other Yes
- 11374 - 13542 (-) CAZyme: GH67 Yes
- 13639 - 15192 (-) CAZyme: GH76 Yes
- 15208 - 16173 (-) other Yes
- 16185 - 18167 (-) other Yes
- 18178 - 21282 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 21316 - 22620 (-) other Yes
- 23111 - 27148 (-) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 Yes
- 27359 - 29641 (+) CAZyme: GH92 Yes
- 29695 - 30645 (+) other Yes
- 30698 - 31855 (+) CAZyme: GH76 Yes
- 31915 - 33360 (+) CAZyme: GH125 Yes
- 33469 - 33735 (+) CDS No

PUL ID

PUL0375

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Title

Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Author

Martens EC, Chiang HC, Gordon JI

Abstract

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profiling and genetic tests were used to define the mechanisms underlying host glycan foraging in vivo and in vitro. PULs that target all major classes of host glycans were identified. However, mucin O-glycans are the principal host substrate foraged in vivo. Simultaneous deletion of five genes encoding ECF-sigma transcription factors, which activate mucin O-glycan utilization, produces defects in bacterial persistence in the gut and in mother-to-offspring transmission. Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology.