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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0386

PubMed

20013800, Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311.

Characterization method

ion trap liquid chromatography,mass spectrometry,target decoy database analysis,high performance anion exchange chromatography

Genomic accession number

NC_011898.1

Nucelotide position range

1505662-1529139

Substrate

hemicellulose

Loci

CCEL_RS06160-CCEL_RS06210

Species

Ruminiclostridium cellulolyticum/1521

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- CCEL_RS06160 WP_015924734.1 0 - 1608 (+) NC_011898.1:1505662-1507270 -
- CCEL_RS06165 WP_015924735.1 1713 - 3339 (+) NC_011898.1:1507375-1509001 -
- CCEL_RS06170 WP_015924736.1 3406 - 4981 (+) NC_011898.1:1509068-1510643 -
- CCEL_RS06175 WP_015924737.1 5003 - 6476 (+) NC_011898.1:1510665-1512138 -
- CCEL_RS06180 WP_015924738.1 6551 - 8792 (+) NC_011898.1:1512213-1514454 -
- CCEL_RS06185 WP_015924739.1 8875 - 10486 (+) NC_011898.1:1514537-1516148 -
- CCEL_RS06190 WP_015924740.1 10560 - 12090 (+) NC_011898.1:1516222-1517752 -
- CCEL_RS06195 WP_015924741.1 12178 - 15046 (+) NC_011898.1:1517840-1520708 -
- CCEL_RS06200 WP_015924742.1 15154 - 16969 (+) NC_011898.1:1520816-1522631 -
- CCEL_RS06205 WP_015924743.1 16998 - 20367 (+) NC_011898.1:1522660-1526029 -
- CCEL_RS06210 WP_015924744.1 20430 - 23478 (+) NC_011898.1:1526092-1529140 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1608 (+) CAZyme: GH43|CBM6|GH43_16 No
- 1714 - 3339 (+) CAZyme: GH10|CBM6 No
- 3407 - 4981 (+) CAZyme: GH43_29|GH43|CBM6 No
- 5004 - 6476 (+) CAZyme: CE1|CBM6 No
- 6552 - 8792 (+) CAZyme: GH43_10|CBM6 No
- 8876 - 10486 (+) CAZyme: GH62|CBM6 No
- 10561 - 12090 (+) CAZyme: GH43_29|GH43|CBM6 No
- 12179 - 15046 (+) CAZyme: GH146|CBM22 No
- 15155 - 16969 (+) CAZyme: GH27|CBM6 No
- 16999 - 20367 (+) CAZyme: GH59|CBM6 No
- 20431 - 23478 (+) CAZyme: GH2|CBM6 No

PUL ID

PUL0386

PubMed

20013800, Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311.

Title

Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses.

Author

Blouzard JC, Coutinho PM, Fierobe HP, Henrissat B, Lignon S, Tardif C, Pages S, de Philip P

Abstract

Clostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate-active enzymes were identified, and their modular structures and activities were predicted. Among them, 62 dockerin-containing proteins bear catalytic modules from numerous carbohydrate-active enzymes' families and whose diversity reflects the chemical and structural complexity of the plant carbohydrate. The composition of the cellulosomes produced by C. cellulolyticum upon growth on different substrates (cellulose, xylan, and wheat straw) was investigated by LC MS/MS. The majority of the proteins encoded by the cip-cel operon, essential for cellulose degradation, were detected in all cellulosome preparations. In the presence of wheat straw, the natural and most complex of the substrates studied, additional proteins predicted to be involved in hemicellulose degradation were produced. A 32-kb gene cluster encodes the majority of these proteins, all harbouring carbohydrate-binding module 6 or carbohydrate-binding module 22 xylan-binding modules along dockerins. This newly identified xyl-doc gene cluster, specialised in hemicellulose degradation, comes in addition of the cip-cel operon for plant cell wall degradation. Hydrolysis efficiencies determined on the different substrates corroborates the finding that cellulosome composition is adapted to the growth substrate.