dbCAN-PUL

PUL ID	PUL0434
PubMed	10613891, J Bacteriol. 2000 Jan;182(1):244-7. doi: 10.1128/JB.182.1.244-247.2000.
Characterization method	SDS-PAGE,Western Blot
Genomic accession number	AF132735.2
Nucelotide position range	91-14184
Substrate	mannooligosaccharide
Loci	engK-hbpA-engL-manA-engM
Species	Clostridium cellulovorans/1493
Degradation or Biosynthesis	degradation

PUL ID	PUL0434
PubMed	10613891, J Bacteriol. 2000 Jan;182(1):244-7. doi: 10.1128/JB.182.1.244-247.2000.
Title	The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA.
Author	Tamaru Y, Doi RH
Abstract	A five-gene cluster around the gene in Clostridium cellulovorans that encodes endoglucanase EngL, which is involved in plant cell wall degradation, has been cloned and sequenced. As a result, a mannanase gene, manA, has been found downstream of engL. The manA gene consists of an open reading frame with 1,275 nucleotides encoding a protein with 425 amino acids and a molecular weight of 47, 156. ManA has a signal peptide followed by a duplicated sequence (DS, or dockerin) at its N terminus and a catalytic domain which belongs to family 5 of the glycosyl hydrolases and shows high sequence similarity with fungal mannanases, such as Agaricus bisporus Cel4 (17.3% identity), Aspergillus aculeatus Man1 (23.7% identity), and Trichoderma reesei Man1 (22.7% identity). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and N-terminal amino acid sequence analyses of the purified recombinant ManA (rManA) indicated that the N-terminal region of the rManA contained a DS and was truncated in Escherichia coli cells. Furthermore, Western blot analysis indicated that ManA is one of the cellulosomal subunits. ManA production is repressed by cellobiose.