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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0446

PubMed

28494314, Carbohydr Res. 2017 Jun 29;446-447:28-31. doi: 10.1016/j.carres.2017.04.024. Epub 2017 May 2.

Characterization method

sequence homology analysis,sugar utilization assay,NMR

Genomic accession number

KY574558.1

Nucelotide position range

1-18810

Substrate

O-antigen

Loci

ARO72880.1-ARO72896.1

Species

Escherichia albertii/208962

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - ARO72880.1 0 - 789 (+) KY574558.1:1-790 -
- - ARO72881.1 1201 - 1582 (+) KY574558.1:1202-1583 -
- - ARO72882.1 1631 - 2456 (+) KY574558.1:1632-2457 -
- - ARO72883.1 2566 - 3607 (+) KY574558.1:2567-3608 -
- - ARO72884.1 3616 - 4891 (+) KY574558.1:3617-4892 -
- - ARO72885.1 4968 - 5547 (+) KY574558.1:4969-5548 -
- - ARO72886.1 5570 - 6656 (+) KY574558.1:5571-6657 -
- - ARO72887.1 6971 - 7223 (+) KY574558.1:6972-7224 -
- - ARO72888.1 7395 - 7749 (+) KY574558.1:7396-7750 -
- - ARO72889.1 7787 - 9071 (+) KY574558.1:7788-9072 -
- - ARO72890.1 9169 - 10369 (+) KY574558.1:9170-10370 -
- - ARO72891.1 10358 - 11477 (+) KY574558.1:10359-11478 -
- - ARO72892.1 11488 - 12553 (+) KY574558.1:11489-12554 -
- - ARO72893.1 12562 - 14392 (+) KY574558.1:12563-14393 -
- - ARO72894.1 14384 - 15620 (+) KY574558.1:14385-15621 -
- - ARO72895.1 15995 - 17402 (+) KY574558.1:15996-17403 -
- - ARO72896.1 17643 - 18810 (+) KY574558.1:17644-18811 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 789 (+) CDS No
- 1202 - 1582 (+) CDS No
- 1632 - 2456 (+) CDS No
- 2567 - 3607 (+) CDS No
- 3617 - 4891 (+) CDS No
- 4969 - 5547 (+) CDS No
- 5571 - 6656 (+) STP: STP|Aminotran_1_2 No
- 6972 - 7223 (+) CDS No
- 7396 - 7749 (+) CDS No
- 7788 - 9071 (+) TC: gnl|TC-DB|G3XD19|2.A.66.2.15 Yes
- 9170 - 10369 (+) other Yes
- 10359 - 11477 (+) CAZyme: GT4 Yes
- 11489 - 12553 (+) CDS No
- 12563 - 14392 (+) CDS No
- 14385 - 15620 (+) CDS No
- 15996 - 17402 (+) CDS No
- 17644 - 18810 (+) CDS No

PUL ID

PUL0446

PubMed

28494314, Carbohydr Res. 2017 Jun 29;446-447:28-31. doi: 10.1016/j.carres.2017.04.024. Epub 2017 May 2.

Title

Structure and gene cluster of the O-antigen of Escherichia albertii O1 resembling the O-antigen of Pseudomonas aeruginosa O5.

Author

Zheng H, Shashkov AS, Xiong Y, Naumenko OI, Wang H, Senchenkova SN, Wang J, Knirel YA

Abstract

The O-specific polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Escherichia albertii serotype O1 strain SP20140089 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure was established for the trisaccharide repeating unit of the O-polysaccharide: -->4)-beta-d-ManpNAc3NAcA-(1 --> 4)-beta-d-GlcpNAm3NAcA-(1 --> 3)-alpha-d-GlcpNAc-(1--> where ManNAc3NAcA and GlcNAm3NAcA indicate 2,3-diacetamido-2,3-dideoxymannuronic acid and 2-acetimidoylamino-3-acetamido-2,3-dideoxyglucuronic acid, respectively. While showing some similarity with O-polysaccharide structures of a group of Pseudomonas aeruginosa serotypes (O2, O5, O16, O18, and O20), that of E. albertii O1 is unique among known bacterial polysaccharide structures. The gene cluster for biosynthesis of the O1-antigen was sequenced and functions of the genes were predicted by comparison with sequences in the available databases, including those involved in the synthesis of nucleotide precursors of 2,3-diamino-2,3-dideoxyhexuronic acid derivatives in P. aeruginosa O5.