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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0451

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Characterization method

sequence homology analysis,sugar utilization assay,NMR,gas chromatography and mass spectrometry

Genomic accession number

CR931683.1

Nucelotide position range

1204-19971

Substrate

O-antigen

Loci

SPC23A_0001-SPC23A_0023

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzg SPC23A_0004 CAI33811.1 1203 - 2649 (+) CR931683.1:2407-3853 -
wzh SPC23A_0005 CAI33812.1 2650 - 3382 (+) CR931683.1:3854-4586 3.1.3.48
wzd SPC23A_0006 CAI33813.1 3390 - 4083 (+) CR931683.1:4594-5287 -
wze SPC23A_0007 CAI33814.1 4092 - 4776 (+) CR931683.1:5296-5980 2.7.10.1
wchA SPC23A_0008 CAI33815.1 4791 - 6159 (+) CR931683.1:5995-7363 -
wchF SPC23A_0009 CAI33816.1 6189 - 7368 (+) CR931683.1:7393-8572 -
wzy SPC23A_0010 CAI33817.1 7577 - 8984 (+) CR931683.1:8781-10188 -
wchV SPC23A_0011 CAI33818.1 8983 - 9955 (+) CR931683.1:10187-11159 -
wchW SPC23A_0012 CAI33819.1 9961 - 11194 (+) CR931683.1:11165-12398 -
wzx SPC23A_0013 CAI33820.1 11195 - 12581 (+) CR931683.1:12399-13785 -
wchX SPC23A_0014 CAI33821.1 12588 - 13737 (+) CR931683.1:13792-14941 -
gtp1 SPC23A_0015 CAI33822.1 13772 - 14801 (+) CR931683.1:14976-16005 -
gtp2 SPC23A_0016 CAI33823.1 14816 - 15521 (+) CR931683.1:16020-16725 -
gtp3 SPC23A_0017 CAI33824.1 15535 - 16369 (+) CR931683.1:16739-17573 -
rmlA SPC23A_0018 CAI33825.1 16528 - 17398 (+) CR931683.1:17732-18602 2.7.7.24
rmlC SPC23A_0019 CAI33826.1 17398 - 17992 (+) CR931683.1:18602-19196 5.1.3.13
rmlB SPC23A_0020 CAI33827.1 18004 - 19054 (+) CR931683.1:19208-20258 4.2.1.46
rmlD SPC23A_0021 CAI33828.1 19119 - 19971 (+) CR931683.1:20323-21175 1.1.1.133

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

wzg 1204 - 2649 (+) CDS No
wzh 2651 - 3382 (+) CDS No
wzd 3391 - 4083 (+) TC: gnl|TC-DB|Q93E03|8.A.3.2.3 Yes
wze 4093 - 4776 (+) TC: gnl|TC-DB|Q93NW3|8.A.3.2.2 Yes
wchA 4792 - 6159 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
wchF 6190 - 7368 (+) CAZyme: GT4 Yes
wzy 7578 - 8984 (+) other Yes
wchV 8984 - 9955 (+) TC: gnl|TC-DB|P75905|4.D.1.1.3 Yes
wchW 9962 - 11194 (+) CDS No
wzx 11196 - 12581 (+) CDS No
wchX 12589 - 13737 (+) CDS No
gtp1 13773 - 14801 (+) CDS No
gtp2 14817 - 15521 (+) CDS No
gtp3 15536 - 16369 (+) CDS No
rmlA 16529 - 17398 (+) CDS No
rmlC 17399 - 17992 (+) CDS No
rmlB 18005 - 19054 (+) CDS No
rmlD 19120 - 19971 (+) CDS No

PUL ID

PUL0451

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Title

Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.

Author

Ravenscroft N, Omar A, Hlozek J, Edmonds-Smith C, Follador R, Serventi F, Lipowsky G, Kuttel MM, Cescutti P, Faridmoayer A

Abstract

Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized. Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B. Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A). The structure of the serotype 23A capsular polysaccharide is: -->4)-beta-D-Glcp-(1-->3)-[[alpha-L-Rhap-(1-->2)]-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)]-beta-L-Rhap-(1-->. This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted beta-Gal is linked to beta-Rha as a side chain. This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus. Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-beta-Gal to resonate in the alpha-anomeric region. The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal alpha-Rha: -->4)-beta-D-Glcp-(1-->4)-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)-beta-L-Rhap-(1-->. The immunodominant terminal alpha-Rha of 23F is more sterically crowded in 23A and absent in 23B. This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.