Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0461

PubMed

31220629, Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6.

Characterization method

sequence homology analysis,sugar utilization assay,NMR

Genomic accession number

NZ_PGLT01000029.1,NZ_PGLT01000002.1

Nucelotide position range

25798-37533,403-1479

Substrate

O-antigen

Loci

CU276_RS20415-CU276_RS20465+CU276_RS02105

Species

Yersinia kristensenii/28152

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

fepE CU276_RS20415 WP_050096215.1 25797 - 26937 (-) NZ_PGLT01000029.1:25798-26937 -
- CU276_RS20420 WP_050096216.1 27090 - 27582 (-) NZ_PGLT01000029.1:27091-27582 -
- CU276_RS20425 WP_050096217.1 27602 - 28736 (-) NZ_PGLT01000029.1:27603-28736 5.1.3.14
- CU276_RS20430 WP_050096218.1 28725 - 29844 (-) NZ_PGLT01000029.1:28726-29844 -
- CU276_RS20435 WP_050096219.1 29846 - 30881 (-) NZ_PGLT01000029.1:29847-30881 -
- CU276_RS20440 WP_100191386.1 30883 - 32092 (-) NZ_PGLT01000029.1:30884-32092 -
- CU276_RS20445 WP_050096221.1 32081 - 33182 (-) NZ_PGLT01000029.1:32082-33182 -
- CU276_RS20450 WP_100191387.1 33178 - 34333 (-) NZ_PGLT01000029.1:33179-34333 -
- CU276_RS20455 WP_050096223.1 34334 - 35729 (-) NZ_PGLT01000029.1:34335-35729 -
- CU276_RS20460 WP_050096224.1 35731 - 36778 (-) NZ_PGLT01000029.1:35732-36778 -
- CU276_RS20465 WP_100191388.1 36774 - 37533 (-) NZ_PGLT01000029.1:36775-37533 -
ompA CU276_RS02105 WP_050094375.1 37970 - 39047 (+) NZ_PGLT01000002.1:403-1479 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 25798 - 26937 (-) CDS No
- 27091 - 27582 (-) CDS No
- 27603 - 28736 (-) CDS No
- 28726 - 29844 (-) CDS No
- 29847 - 30881 (-) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 30884 - 32092 (-) CAZyme: GT4 Yes
- 32082 - 33182 (-) other Yes
- 33179 - 34333 (-) other Yes
- 34335 - 35729 (-) other Yes
- 35732 - 36778 (-) CAZyme: GT4 Yes
- 36775 - 37533 (-) CAZyme: GT4 Yes
- 37971 - 39047 (+) TC: gnl|TC-DB|P0A910|1.B.6.1.1 No

PUL ID

PUL0461

PubMed

31220629, Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6.

Title

Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134.

Author

Sizova OV, Shashkov AS, Toukach PV, Knirel YA, Shaikhutdinova RZ, Ivanov SA, Kislichkina AA, Dentovskaya SV

Abstract

Mild acid degradation of the lipopolysaccharide of Yersinia kristensenii C-134 afforded a glycerol teichoic acid-like O-polysaccharide, which was studied by sugar analysis, O-deacetylation and dephosphorylation along with 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: This structure is related to those of other Y. kristensenii O-polysaccharides studied earlier. The O-antigen gene cluster of Y. kristensenii capital ES, Cyrillic-134 was analyzed and found to be consistent with the O-polysaccharide structure established.