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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0519

PubMed

8244973, J Biol Chem. 1993 Dec 5;268(34):25402-8.

Characterization method

gene deletion mutant and growth assay

Genomic accession number

NC_003098.1

Nucelotide position range

1895736-1905654

Substrate

maltose

Loci

MalRADCXMP

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

malP spr1916 NP_359507.1 0 - 2259 (-) NC_003098.1:1895736-1897995 2.4.1.1
malM spr1917 NP_359508.1 2284 - 3802 (-) NC_003098.1:1898020-1899538 2.4.1.25
malX spr1918 NP_359509.1 4351 - 5623 (+) NC_003098.1:1900087-1901359 -
malC spr1919 NP_359510.1 5718 - 7026 (+) NC_003098.1:1901454-1902762 -
malD spr1920 NP_359511.1 7006 - 7870 (+) NC_003098.1:1902742-1903606 -
malA spr1921 NP_359512.1 8122 - 8923 (+) NC_003098.1:1903858-1904659 -
malR spr1922 NP_359513.1 8932 - 9919 (+) NC_003098.1:1904668-1905655 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

malP 1 - 2259 (-) CAZyme: GT35 Yes
malM 2285 - 3802 (-) CAZyme: GH77 Yes
malX 4352 - 5623 (+) TC: gnl|TC-DB|O06989|3.A.1.1.26 Yes
malC 5719 - 7026 (+) TC: gnl|TC-DB|O06990|3.A.1.1.26 Yes
malD 7007 - 7870 (+) TC: gnl|TC-DB|O06991|3.A.1.1.26 Yes
malA 8123 - 8923 (+) CDS No
malR 8933 - 9919 (+) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No

PUL ID

PUL0519

PubMed

8244973, J Biol Chem. 1993 Dec 5;268(34):25402-8.

Title

Characterization of the Streptococcus pneumoniae maltosaccharide regulator MalR, a member of the LacI-GalR family of repressors displaying distinctive genetic features.

Author

Puyet A, Ibanez AM, Espinosa M

Abstract

The gene encoding a transcriptional repressor of the maltosaccharide utilization operons of the Gram-positive bacterium Streptococcus pneumoniae (malR) has been cloned and sequenced. The genetic structure of the locus reveals the presence of an upstream gene necessary for growth on maltotetraose medium (malA). The phenotype of malR- and malA- mutants obtained by interruption of the coding regions suggests that both genes could belong to the same transcription unit. Two copies of a DNA motif consisting of three conserved regions of 59, 42, and 49 nucleotides were found located upstream and downstream of the malA-malR putative operon. These DNA structures are almost identical to the reported Box sequences associated with several genes of S. pneumoniae. The protein encoded by malR was visualized and partially purified after selective expression in Escherichia coli, whereas the product of malA was identified in vitro. The amino acid sequence of MalR displays similarities with the Lac and Gal family of repressors. The highest similarities were found when comparing MalR with the E. coli MalI repressor, which is related with an indirect induction pathway of the maltose regulon. The significance of these similarities is discussed in terms of the possible evolutionary pathways followed by structural and regulatory genes of sugar utilization systems in bacteria.