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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0574

PubMed

16822234, Biochem J. 2006 Oct 15;399(2):241-7. doi: 10.1042/BJ20060307.

Characterization method

enzyme activity assay

Genomic accession number

NC_002737.2

Nucelotide position range

1312429-1324390

Substrate

alpha-mannoside,N-glycan

Loci

spy_1593-spy_1604

Species

Streptococcus pyogenes/1314

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- SPY_RS06615 WP_002988998.1 0 - 927 (-) NC_002737.2:1312429-1313356 -
- SPY_RS06620 WP_011285651.1 936 - 1887 (-) NC_002737.2:1313365-1314316 -
- SPY_RS06625 WP_011285652.1 2082 - 2961 (+) NC_002737.2:1314511-1315390 -
- SPY_RS06630 WP_010922527.1 3571 - 5014 (-) NC_002737.2:1316000-1317443 -
- SPY_RS06635 WP_010922528.1 5037 - 6732 (-) NC_002737.2:1317466-1319161 -
- SPY_RS06640 WP_002983707.1 6782 - 7823 (-) NC_002737.2:1319211-1320252 -
- SPY_RS06645 WP_011285655.1 7955 - 9242 (+) NC_002737.2:1320384-1321671 -
- SPY_RS06650 WP_010922530.1 9256 - 11962 (+) NC_002737.2:1321685-1324391 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 927 (-) TC: gnl|TC-DB|A9QDR8|3.A.1.1.29 Yes
- 937 - 1887 (-) TC: gnl|TC-DB|Q09LY7|3.A.1.1.9 Yes
- 2083 - 2961 (+) other Yes
- 3572 - 5014 (-) CAZyme: GH1 Yes
- 5038 - 6732 (-) CAZyme: GH84 Yes
- 6783 - 7823 (-) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0037767 Yes
- 7956 - 9242 (+) CAZyme: GH125 Yes
- 9257 - 11962 (+) CAZyme: GH38 Yes

PUL ID

PUL0574

PubMed

16822234, Biochem J. 2006 Oct 15;399(2):241-7. doi: 10.1042/BJ20060307.

Title

Functional analysis of a group A streptococcal glycoside hydrolase Spy1600 from family 84 reveals it is a beta-N-acetylglucosaminidase and not a hyaluronidase.

Author

Sheldon WL, Macauley MS, Taylor EJ, Robinson CE, Charnock SJ, Davies GJ, Vocadlo DJ, Black GW

Abstract

Group A streptococcus (Streptococcus pyogenes) is the causative agent of severe invasive infections such as necrotizing fasciitis (the so-called 'flesh eating disease') and toxic-shock syndrome. Spy1600, a glycoside hydrolase from family 84 of the large superfamily of glycoside hydrolases, has been proposed to be a virulence factor. In the present study we show that Spy1600 has no activity toward galactosaminides or hyaluronan, but does remove beta-O-linked N-acetylglucosamine from mammalian glycoproteins--an observation consistent with the inclusion of eukaryotic O-glycoprotein 2-acetamido-2-deoxy-beta-D-glucopyranosidases within glycoside hydrolase family 84. Proton NMR studies, structure-reactivity studies for a series of fluorinated analogues and analysis of 1,2-dideoxy-2'-methyl-alpha-D-glucopyranoso-[2,1-d]-Delta2'-thiazoline as a competitive inhibitor reveals that Spy1600 uses a double-displacement mechanism involving substrate-assisted catalysis. Family 84 glycoside hydrolases are therefore comprised of both prokaryotic and eukaryotic beta-N-acetylglucosaminidases using a conserved catalytic mechanism involving substrate-assisted catalysis. Since these enzymes do not have detectable hyaluronidase activity, many family 84 glycoside hydrolases are most likely incorrectly annotated as hyaluronidases.