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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0581

PubMed

22286980, Appl Environ Microbiol. 2012 Apr;78(7):2493-5. doi: 10.1128/AEM.07442-11. Epub 2012 Jan 27.

Characterization method

SDS-PAGE,enzyme activity assay

Genomic accession number

AB669415

Nucelotide position range

174-4818

Substrate

inulin

Loci

sacA-pfk-inu

Species

Microbulbifer sp. JAM-3301/1081041

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

sacA - BAL70276.1 0 - 1458 (+) AB669415.1:174-1632 -
pfk - BAL70277.1 1468 - 2422 (+) AB669415.1:1642-2596 2.7.1.11
inu - BAL70278.1 2425 - 4645 (+) AB669415.1:2599-4819 3.2.1.7

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1458 (+) CAZyme: GH32|CBM38 No
- 1469 - 2422 (+) STP: STP|PfkB No
- 2426 - 4645 (+) CAZyme: GH32 No

PUL ID

PUL0581

PubMed

22286980, Appl Environ Microbiol. 2012 Apr;78(7):2493-5. doi: 10.1128/AEM.07442-11. Epub 2012 Jan 27.

Title

Cloning and sequencing of inulinase and beta-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes.

Author

Kobayashi T, Uchimura K, Deguchi S, Horikoshi K

Abstract

An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a beta-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.