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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0590

PubMed

23951303, PLoS One. 2013 Aug 7;8(8):e72285. doi: 10.1371/journal.pone.0072285. eCollection 2013.

Characterization method

qRT-PCR,gene deletion mutant and growth assay,microarray

Genomic accession number

NZ_ABQJ01000017.1

Nucelotide position range

21176-27433

Substrate

maltose,maltodextrin

Loci

EFME1162_RS11430-EFME1162_RS11450

Species

Enterococcus faecium/1352

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- EFME1162_RS11430 WP_002295923.1 0 - 816 (-) NZ_ABQJ01000017.1:21176-21992 -
- EFME1162_RS11435 WP_002288156.1 828 - 1680 (-) NZ_ABQJ01000017.1:22004-22856 -
- EFME1162_RS11440 WP_002288157.1 1682 - 2984 (-) NZ_ABQJ01000017.1:22858-24160 -
- EFME1162_RS11445 WP_002288159.1 3130 - 4390 (-) NZ_ABQJ01000017.1:24306-25566 -
- EFME1162_RS11450 WP_002288160.1 4491 - 6258 (-) NZ_ABQJ01000017.1:25667-27434 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 816 (-) CDS No
- 829 - 1680 (-) TC: gnl|TC-DB|O06991|3.A.1.1.26 Yes
- 1683 - 2984 (-) TC: gnl|TC-DB|O06990|3.A.1.1.26 Yes
- 3131 - 4390 (-) TC: gnl|TC-DB|O06989|3.A.1.1.26 Yes
- 4492 - 6258 (-) CAZyme: CBM34|GH13_20 Yes

PUL ID

PUL0590

PubMed

23951303, PLoS One. 2013 Aug 7;8(8):e72285. doi: 10.1371/journal.pone.0072285. eCollection 2013.

Title

A LacI-family regulator activates maltodextrin metabolism of Enterococcus faecium.

Author

Zhang X, Rogers M, Bierschenk D, Bonten MJ, Willems RJ, van Schaik W

Abstract

Enterococcus faecium is a gut commensal of humans and animals. In the intestinal tract, E. faecium will have access to a wide variety of carbohydrates, including maltodextrins and maltose, which are the sugars that result from the enzymatic digestion of starch by host-derived and microbial amylases. In this study, we identified the genetic determinants for maltodextrin utilization of E. faecium E1162. We generated a deletion mutant of the mdxABCD-pulA gene cluster that is homologous to maltodextrin uptake genes in other Gram-positive bacteria, and a deletion mutant of the mdxR gene, which is predicted to encode a LacI family regulator of mdxABCD-pulA. Both mutations impaired growth on maltodextrins but had no effect on the growth on maltose and glucose. Comparative transcriptome analysis showed that eight genes (including mdxABCD-pulA) were expressed at significantly lower levels in the isogenic DeltamdxR mutant strain compared to the parental strain when grown on maltose. Quantitative real-time RT-PCR confirmed the results of transcriptome analysis and showed that the transcription of a putative maltose utilization gene cluster is induced in a semi-defined medium supplemented with maltose but is not regulated by MdxR. Understanding the maltodextrin metabolism of E. faecium could yield novel insights into the underlying mechanisms that contribute to the gut commensal lifestyle of E. faecium.