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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0649

PubMed

33667610, Res Microbiol. 2021 Apr-May;172(3):103815. doi: 10.1016/j.resmic.2021.103815. Epub 2021 Mar 3.

Characterization method

Smith degradation,NMR

Genomic accession number

MN958100.1

Nucelotide position range

1-25980

Substrate

capsule polysaccharide

Loci

wzc-wzb-wza-gna-mIB-mID-mIA-mIC-gtr164-wzx-gtr165-wzy-orf-gtr166-itrA4-wzi-galU-ugd-gpi-gne1-pgt1-pgm

Species

Acinetobacter baumannii B8300/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzc - QPD01053.1 0 - 2196 (-) MN958100.1:1-2197 -
wzb - QPD01054.1 2217 - 2646 (-) MN958100.1:2218-2647 -
wza - QPD01055.1 2647 - 3829 (-) MN958100.1:2648-3830 -
gna - QPD01056.1 3952 - 5230 (+) MN958100.1:3953-5231 -
rmlB - QPD01057.1 5252 - 6329 (+) MN958100.1:5253-6330 -
rmlD - QPD01058.1 6345 - 7254 (+) MN958100.1:6346-7255 -
rmlA - QPD01059.1 7250 - 8141 (+) MN958100.1:7251-8142 -
rmlC - QPD01060.1 8209 - 8785 (+) MN958100.1:8210-8786 -
gtr164 - QPD01061.1 8793 - 9690 (+) MN958100.1:8794-9691 -
wzx - QPD01062.1 9686 - 10973 (+) MN958100.1:9687-10974 -
gtr165 - QPD01063.1 10983 - 11883 (+) MN958100.1:10984-11884 -
wzy - QPD01064.1 11907 - 13170 (+) MN958100.1:11908-13171 -
- - QPD01065.1 13163 - 14420 (-) MN958100.1:13164-14421 -
gtr166 - QPD01066.1 14431 - 15268 (-) MN958100.1:14432-15269 -
itrA4 - QPD01067.1 15269 - 15968 (-) MN958100.1:15270-15969 -
wzi_KL - QPD01068.1 16181 - 17630 (+) MN958100.1:16182-17631 -
galU - QPD01069.1 17749 - 18637 (+) MN958100.1:17750-18638 -
ugd - QPD01070.1 18652 - 19918 (+) MN958100.1:18653-19919 -
gpi - QPD01071.1 19914 - 21588 (+) MN958100.1:19915-21589 -
gne1 - QPD01072.1 21580 - 22600 (+) MN958100.1:21581-22601 -
pgt1 - QPD01073.1 22740 - 24582 (+) MN958100.1:22741-24583 -
pgm - QPD01074.1 24609 - 25980 (-) MN958100.1:24610-25981 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2196 (-) TC: gnl|TC-DB|Q45409|8.A.3.3.3 Yes
- 2218 - 2646 (-) other Yes
- 2648 - 3829 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 3953 - 5230 (+) other Yes
- 5253 - 6329 (+) other Yes
- 6346 - 7254 (+) other Yes
- 7251 - 8141 (+) other Yes
- 8210 - 8785 (+) other Yes
- 8794 - 9690 (+) CAZyme: GT2|GT2 Yes
- 9687 - 10973 (+) TC: gnl|TC-DB|P37746|2.A.66.2.1 Yes
- 10984 - 11883 (+) CAZyme: GT2 Yes
- 11908 - 13170 (+) other Yes
- 13164 - 14420 (-) other Yes
- 14432 - 15268 (-) CAZyme: GT2 Yes
- 15270 - 15968 (-) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 16182 - 17630 (+) TC: gnl|TC-DB|Q5MQC2|1.B.73.1.1 Yes
- 17750 - 18637 (+) CDS No
- 18653 - 19918 (+) CDS No
- 19915 - 21588 (+) CDS No
- 21581 - 22600 (+) CDS No
- 22741 - 24582 (+) CDS No
- 24610 - 25980 (-) CDS No

PUL ID

PUL0649

PubMed

33667610, Res Microbiol. 2021 Apr-May;172(3):103815. doi: 10.1016/j.resmic.2021.103815. Epub 2021 Mar 3.

Title

A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.

Author

Senchenkova SN, Shashkov AS, Shneider MM, Popova AV, Balaji V, Biswas I, Knirel YA, Kenyon JJ

Abstract

The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional (1)H and (13)C NMR spectroscopy. The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar. Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier. However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier. The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus. This module also includes an unknown open reading frame (orf(KL92)), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface. Investigation into the origins of orf(KL92)-gtr166-itrA4-wzi(KL92) revealed it might have originated from Acinetobacter junii.