| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0010 | enzyme activity assay, liquid chromatography and mass spectrometry | xylan | Geobacillus thermodenitrificans | 28616644 Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14. |
2017 Aug | degradation | 42 | 9 | GH43, GH43_11, GH10, GH39, GH67, GH10, CBM22, GH52, CE4, GH51 |
| PUL0013 | Northern Blot, RT-PCR, isothermal titration calorimetry, electrophoretic mobility shift assay | arabinan | Geobacillus stearothermophilus | 21460081 The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1. |
2011 Jun | degradation | 56 | 11 | GH43_5, GH43, GH43, GH43_4, GH51, GH51, GH127, CE4, GH52, GH10, GH67, GH39, GH43_11 |
| PUL0061 | NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 31421354 Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8. |
2019 Oct 1 | biosynthesis | 35 | 4 | GT2, GT2, GT4, GT2, GT4, CE4 |
| PUL0070 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 36 | 3 | GT52, GT0, GT14, GT2, GT25, GT4, CE4 |
| PUL0071 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 38 | 4 | GT0, GT14, GT2, GT25, GT4, CE4 |
| PUL0076 | sequence homology analysis | capsule polysaccharide, outer core capsule polysaccharide | Acinetobacter baumannii | 31396168 Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019. |
2019 | biosynthesis | 7 | 7 | GT4, CE4 |
| PUL0077 | sequence homology analysis | capsule polysaccharide, outer core capsule polysaccharide | Acinetobacter baumannii | 31396168 Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019. |
2019 | biosynthesis | 11 | 7 | GT4, CE4 |
| PUL0079 | sequence homology analysis | capsule polysaccharide, outer core capsule polysaccharide | Acinetobacter baumannii | 31396168 Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019. |
2019 | biosynthesis | 16 | 6 | CE4, GT4, GT2 |
| PUL0080 | sequence homology analysis | capsule polysaccharide, outer core capsule polysaccharide | Acinetobacter baumannii | 31396168 Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019. |
2019 | biosynthesis | 12 | 6 | CE4, GT4, GT2 |
| PUL0135 | enzyme activity assay, substrate binding assay | pectin | Pseudoalteromonas sp. | 30341080 Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Appl Environ Microbiol. 2018 Dec 13;85(1):e02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1. |
2019 Jan 1 | degradation | 21 | 8 | CE12, CE4, GH28, GH105, GH105, GH43, GH43_10, CE8, PL1_2 |
| PUL0191 | RNA-Seq, RT-PCR, qPCR | pectin | Bacteroides xylanisolvens | 26920945 Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1. |
2016 Feb 27 | degradation | 11 | 5 | GH105, CE8, CE8, CE12, CE4, PL1_2, PL1_2 |
| PUL0270 | comparative genomics | exopolysaccharide, lipopolysaccharide | Nitrosospira multiformis | 18390676 Complete genome sequence of Nitrosospira multiformis, an ammonia-oxidizing bacterium from the soil environment. Appl Environ Microbiol. 2008 Jun;74(11):3559-72. doi: 10.1128/AEM.02722-07. Epub 2008 Apr 4. |
2008 Jun | biosynthesis | 15 | 3 | GT4, CE0, CE4 |
| PUL0305 | RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay | homogalacturonan | Bacteroides thetaiotaomicron | 29255254 Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18. |
2018 Feb | degradation | 17 | 7 | GH105, CE8, CE4, CE8, CE12, PL1_2, PL1, CE8, PL1_2, PL1, CE8, PL1_2, GH28 |
| PUL0334 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 23 | 5 | GH9, GH31, GH9, GH9, CE4 |
| PUL0381 | microarray, gene deletion mutant and growth assay | chitin | Vibrio cholerae | 14983042 The Vibrio cholerae chitin utilization program. Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2524-9. doi: 10.1073/pnas.0308707101. |
2004 Feb 24 | degradation | 6 | 1 | CBM12, CE4, GH4 |
| PUL0428 | transposon mutagenesis, growth assay | exopolysaccharide | Staphylococcus epidermidis | 8809760 Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol. 1996 Jun;20(5):1083-91. doi: 10.1111/j.1365-2958.1996.tb02548.x. |
1996 Jun | biosynthesis | 5 | 1 | CE4 |
| PUL0483 | growth assay | pectin | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 12 | 5 | GH2, CE12, CE4, GH28, GH117, GH106, GH105 |
| PUL0503 | sequence homology analysis, microscopy | capsule polysaccharide | Bacteroides fragilis | 20829291 Twenty-eight divergent polysaccharide loci specifying within- and amongst-strain capsule diversity in three strains of Bacteroides fragilis. Microbiology (Reading). 2010 Nov;156(Pt 11):3255-3269. doi: 10.1099/mic.0.042978-0. Epub 2010 Sep 9. |
2010 Nov | biosynthesis | 16 | 6 | GT11, GT4, GT2, GT4, CE4, CE0, GT4, GT2 |
| PUL0529 | microarray, qPCR | homogalacturonan | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 27 | 13 | GH105, CE8, CE12, CE4, CE8, PL1, CE8, PL1_2, PL1, PL1_2, PL1, PL1_2, CE8, PL1, PL1_2, PL1, CE8, PL1_2, CE8, GH28, GH3, GH43_10, CBM6 |
| PUL0542 | substrate binding assay | xylan | Geobacillus stearothermophilus | 10368143 The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6. J Bacteriol. 1999 Jun;181(12):3695-704. doi: 10.1128/JB.181.12.3695-3704.1999. |
1999 Jun | degradation | 30 | 6 | CE4, GH52, GH10, GH67, GH39, GH43_11 |
| PUL0564 | microarray, qPCR | rhamnogalacturonan | Bacteroides thetaiotaomicron | 18996345, 22205877 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2008 Nov 13,2011 Dec | degradation | 39 | 23 | GH106, GH28, GH28, CE12, GH2, GH43, GH43_18, GH42, GH28, CE4, CE0, GH28, GH2, GH27, CBM35, GH35, PL9, PL9_1, PL26, CE12, GH105, PL11, PL11_1, GH105, CE4, CE0, CE6, GH2, PL9_1 |
| PUL0602 | sequence homology analysis | xylan | Parageobacillus thermoglucosidasius | 26442136 Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park. Stand Genomic Sci. 2015 Oct 5;10:73. doi: 10.1186/s40793-015-0031-z. eCollection 2015. |
2015 | degradation | 26 | 6 | CBM22, GH10, GH39, GH67, GH10, GH52, CE4 |
Copyright 2020 © YIN LAB, UNL. All rights reserved. Designed by Catie Ausland and Jinfang Zheng. Maintained by Yanbin Yin.